Leica M165 FC
Leica M205 FA
User Manual
Download from Www.Somanuals.com. All Manuals Search And Download.
Important Safety Notes
User Manual
You can combine individual system articles
with articles from external suppliers (e.g. cold
light sources, etc.). Please read the user manual
and the safety requirements of the supplier.
Special manuals
Special manuals are provided for a number of
accessories:
The individual modules of the Leica M stereo-
microscopy series include an interactive
CD-ROM with all relevant user manuals in
20 other languages. Keep it in a safe place,
and readily accessible to the user. User manu-
als and updates are also available for you
to download and print from our web site at
www.stereomicroscopy.com.
ρ
User Manual M2-216-1 for lamp housing
Before installing, operating or using the instru-
ments, read the user manuals listed above.
In particular, please observe all safety instruc-
tions.
with high-pressure mercury burner.
ρ
User Manual for ebq 100 isolated supply
unit for 100W high-pressure mercury
burners.
This User Manual describes the special func-
tions of the individual modules of the Leica M
stereomicroscopy series and contains impor-
tant instructions for their operational safety,
maintenance, and accessories.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
ρ
User Manual M2-267-1 for the motorized
focus system.
ρ
User Manual M2-166-2 for FluoCombi III™.
The "Safety concept" booklet contains addi-
tional safety information regarding the service
work, requirements and the handling of the
stereomicroscope, accessories and electrical
accessories as well as general safety instruc-
tions.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
3
Symbols Used
Warning of a danger
Danger due to hot surface
This symbol warns against touching
accessible hot surfaces, e.g. those of
light bulbs.
This symbol indicates especially impor-
tant information that must be read and
•
complied with.
Failure to comply can cause the following:
ρ
Hazards to personnel
Functional disturbances or damaged instru-
ments
Important information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
ρ
Warning of hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe.
Explanatory notes
ρ
This symbol within the text stands for addi-
tional information and explanations.
Failure to comply can cause the following:
Figures
ρ
Hazards to personnel
Functional disturbances or damaged instru-
ments
(1) Numbers in parentheses within the
descriptions relate to the figures and the
items within those figures.
ρ
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
4
Safety Instructions
Description
are outside of the scope of this manual.
Responsibilities of person in charge of
instrument
The individual modules fulfill the highest
requirements for observation and documenta-
tion of Leica stereomicroscopes of the M series.
Unauthorized alterations to the instrument or
noncompliant use shall void all rights to any
warranty claims.
▶
Refer to "Safety Concept" booklet
Ensure that:
ρ
Intended use
The
M
series stereomicroscopes and
▶
Refer to "Safety Concept" booklet
Place of use
accessories are operated, maintained and
repaired by authorized and trained person-
nel only.
▶
Refer to "Safety Concept" booklet
ρ
Non-intended use
Refer to "Safety Concept" booklet
Electrical components must be placed at
least 10 cm away from the wall and from
flammable substances.
ρ
▶
All operators have read, understood and
observe this User Manual, and particularly
the safety instructions.
Never use M series microscopes or their compo-
nents for surgical procedures (e.g. on the eye)
unless they are specifically intended for that
purpose.
ρ
ρ
Avoid large temperature fluctuations,
direct sunlight and vibrations. These condi-
tions can distort measurements and micro-
graphic images.
The instruments and accessories described in
this operating manual have been tested for
safety and potential hazards. The responsible
Leica affiliate must be consulted whenever
the instrument is altered, modified or used in
conjunction with non-Leica components that
In warm and warm-damp climatic zones,
the individual components require special
care in order to prevent the build-up of
fungus.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
5
Safety Instructions (cont'd.)
Repairs, service work
Integration in third-party products
▶ Refer to "Safety Concept" booklet
this reason, appropriate measures for reduction
of the workload must be taken:
▶
Refer to "Safety Concept" booklet
ρ
Only original Leica Microsystems spare
parts may be used.
ρ
ρ
ρ
Optimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently).
Disposal
▶
Refer to "Safety Concept" booklet
ρ
Before opening the instruments, switch off
the power and unplug the power cable.
Thorough training of the personnel, giving
consideration to ergonomic and organiza-
tional aspects.
Legal requirements
Touching the live circuit can cause
injury.
▶
Refer to "Safety Concept" booklet
The ergonomic design and construction
of the Leica M stereomicroscopy series are
intended to reduce the exertion of the user
to a minimum.
EC Declaration of Conformity
ρ
Refer to "Safety Concept" booklet
Transport
ρ
Use the original packaging for shipping or
transporting the individual modules of the
Leica M stereomicroscopy series and the
accessory components.
Health risks
Workplaces with stereomicroscopes
•
facilitate and improve the viewing task,
ρ
but they also impose high demands on the eyes
and holding muscles of the user. Depending on
the duration of uninterrupted work, asthenopia
and musculoskeletal problems may occur. For
In order to prevent damage from vibrations,
disassemble all moving parts that (accord-
ing to the user manual) can be assembled
and disassembled by the customer and
pack them separately.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
6
Safety Instructions (cont'd.)
Direct contact with eyepieces is a poten-
tial transmission method for bacterial
Light sources: Safety regulations
Protective measures of the manufacturer:
Warning
UV radiation could damage the eyes.
•
ρ
and viral infections of the eye.
UV protection screen in front of the speci-
men plane prevents the user from looking
directly into the UV rays.
•
Therefore:
ρ
ρ
ρ
ρ
The risk can be kept to a minimum by using
personal eyepieces for each individual or
detachable eyecups.
Never look into the light spot on the speci-
men plane without a UV protection screen.
ρ
Dummy filter carriers in the free positions
of the rapid filter changer prevent direct UV
radiation from reaching the eyes.
Never look into the eyepieces if no excita-
tion filter is in the beam path.
ρ
UV filters are installed in the observation
beam paths to protect the eyes.
Fill empty filter positions with dummy filter
carriers (e.g. M165 FC).
ρ
The stray-light protection on the lamp
housing prevents irradiation of the hands.
Do not select a white, strongly reflective
background for the specimen.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
7
Safety Instructions (cont'd.)
Supply unit
Always unplug the supply unit from the power
supply:
Lamp housing
Never open the lamp housing while the
lamp is switched on. Risk of explosion,
UV exposure, blinding!
Mercury lamp
ρ
Follow the user manual and safety instruc-
•
tions provided by the lamp manufacturer,
and, in particular, the section on how to
proceed if a lamp breaks and releases
mercury.
ρ
When installing and disassembling the
lamp housing
Before opening the lamp housing, allow
it to cool off for at least 15 minutes.
Danger of explosion!
ρ
Before opening the lamp housing
•
ρ
ρ
For transport, remove the mercury lamp,
transport it in its original packaging and
protect moving parts in the lamp housing
using the transport anchors.
ρ
When replacing the high-pressure mercury
lamp and other parts, such as the heat-
absorbing filter or the collector
Never cover the air duct on the lamp
housing. Danger of fire!
•
ρ
During maintenance work on the supply
When it has reached the end of its rated life
(follow the manufacturer's specifications
and the minute meter on the supply unit).
unit
ρ
ρ
To minimize the risk of explosion, replace a
discolored mercury lamp promptly.
Leica Microsystems assumes no liability
for damage caused by exploding, incor-
rectly installed or improperly used mercury
lamps.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
8
Contents
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
9
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
10
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
11
Congratulations!
Congratulations on obtaining your new Leica M series stereomicroscope.
We are convinced it will exceed your expectations, as never before have
we applied our decades of experience in the areas of optics, mechanical
engineering and ergonomics in such an uncompromising manner.
Though the reliability and robustness of Leica stereomicroscopes is
legendary, like any high-tech product, the Leica M series requires a certain
degree of care and attention. Therefore, we recommend that you read
this manual. It contains all the information you need regarding operation,
safety and maintenance. Simply observing a few guidelines will ensure
that even after years of intensive use, your stereomicroscope will continue
to work as smoothly and reliably as on the very first day.
The Leica M series embodies all the qualities you associate with the name
Leica Microsystems: excellent objectives, high-quality engineering, and
reliability. Furthermore, the modular design ensures that the M series
adapts perfectly to your needs—no matter which accessories you require
for your tasks.
We wish you the best of success in your work—after all, you are now
equipped with the best tool!
The entire imaging system, including the zoom, objective and ErgoTube®,
is apochromatically corrected with much technological effort. Contrast,
sharpness, richness in detail, resolution, image and color fidelity are opti-
mum. Inaddition, thepatentedilluminationbeampathguaranteesatevery
zoomlevelthatlightutilizationisatamaximumandthatfluorescenceimages
are intensely luminous on a jet black background.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
13
A Step Towards Infinity
Ever since their introduction by Horatio S.
Greenough, stereomicroscopes have worked
according to the optical principles based
primarily on Ernst Abbe's research. For over a
century, ingenious optics designers and engi-
neers have worked to push magnification, reso-
lution and image fidelity to the limit permitted
by optics.
Limits are made to be broken
The Leica M205 FA is the world's first fluores-
cence stereomicroscope with a zoom range
of 20.5:1. This accomplishment, however, was
not enough for Leica's engineers. With the new
FusionOptic™ in the Leica M205 FA, they have
succeeded in going yet another step beyond
previous limits. In addition to the increase in
magnification, the resolution, too, has been
increased to up to 1050 lp / mm, which corre-
sponds to a resolved structure size of 472 nm.
In doing so, they have always been constrained
by the interrelation between three factors: the
higher a microscope's resolution, the lower the
available working distance. If one increases the
distance of the optical axes, the three-dimen-
sional image seen by the observer becomes
distorted—a sphere becomes an ellipse, a flat
surface curves towards the observer.
Of course, this increase in performance benefits
your everyday work. Set up your specimens on
the microscope table with comfortable freedom
of movement and discover details in stereomi-
croscopy that you could never see before.
* Leica M205 FA with 1.0× planapochromat and 10× eyepieces
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
14
The Electronics: Comfort, Convenience and Safety for Your Experiments
Never before have electronics been used as
extensively in a Leica series as in the new M
series lineup. Optics carrier, stand, base and
illuminator are all connected using electrical
contacts—which provides a number of advan-
tages.
Reliability for your experiments
diaphragm and more and transmits the infor-
mation to the Leica LAS software. Thus you
always know the conditions under which an
image was acquired.
Exact reproducibility of results is an increas-
ingly common demand, particularly in research
and development. The continuous encoding
captures parameters such as the magnifica-
tion, the illumination, the position of the iris
Fewer cables
A large part of the cables have been routed in
the interior of the column. Data are also trans-
ferred via the interface between the column,
the optics carrier and the carrier. The direct
result is that you need fewer cables—this not
only makes your workstation neater and more
comfortable, it even makes it appear larger.
Contacts not only transmit data, but also supply
the power.
Leica Application Suite (LAS) evaluates the
transmitted data and can restore the test situa-
tion later.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
15
The Modular Design: Everything is Relative
The Leica M series provides maximum flexibil-
ity in choosing equipment, thanks primarily to
the modular configuration and the compatibil-
ity that Leica has painstakingly maintained for
decades. The optics carriers, eyepieces, bases
and more can be combined in any way you
choose, allowing you to create the microscope
that best suits your needs.
Despite this, you will notice that the controls
Have a special request? Let us know!
Leica Microsystems enjoys an exceptional repu-
tation when it comes to devising customer-
specific solutions. If you have a special request
that cannot be met with standard parts, contact
your Leica consultant. We have a solution for
every problem.
and individual components do not differ signif-
icantly. Whichever configuration you choose,
you will quickly feel right at home.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
16
Maximum Compatibility
Leica engineers were careful to ensure that the
new Leica M series—like its predecessors—
remains compatible with existing series. This
means that objectives, bases, tubes and so on
can be reused.
Tubes
The interface between the optics carrier and the
tube has remained the same, so existing tubes
fit the new M series. The new tubes are designed
for eyepieces with field number 23, while the
predecessor models were only designed for field
number 21, resulting in a smaller object field.
Objectives
All new objectives of the Leica M series are
parfocal, meaning that when used with the
objective nosepiece, they can be replaced while
keeping the specimen in perfect focus.
Eyepieces
However, the new M-series eyepieces have an
audible and tangible click to provide immedi-
ate feedback in case of accidental adjustment.
If you prefer, you can continue to use the previ-
ous Leica objective series. In this case, parfocal-
ity is no longer guaranteed.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
17
On We Go
If your new Leica microscope has already been
assembled and commissioned by your Leica
consultant, click here to skip through the instal-
lation instructions and go directly to the Quick
Start Guide on Page 63.
If, on the other hand, you are assembling
the microscope yourself, continue with the
For everything you need to know about the
correct use of fluorescence-related parts, refer to
Page 114.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
18
Assembling the Focusing Column for MDG Bases
The first step is to connect the focusing column
of the M series to the corresponding base.
Assembling the column adapter
1. Securely install the column adapter on the
column using the four included screws.
Assembling the focusing column
2. Securely screw the focusing column to the
base using the six included screws.
Tools used
ρ
Hex socket screwdriver, 3 mm
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
20
Assembling the Focusing Column with an Incident-light Base
When using an incident-light base, the focus-
ing column and motorized focus are installed
directly on the base; no extension plate is
required.
Assembly
3. Screw the base securely onto the focusing
drive.
1. Place the focusing column on the side.
Tools used
ρ
Hex socket screwdriver, 3 mm
2. Insert the four screws provided into the
outer holes of the base.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
21
Motorized Focus: Restricting the Travel Path
Depending on the work situation, it is
useful to restrict the maximum travel
path of the stereomicroscope. This prevents
injury during handling of the sample caused
by fingers or hands becoming trapped or the
specimen touching the objective or even being
damaged by it.
Restricting the bottom travel range
1. Move the motorized focus into the lowest
position you want to reach.
3. Push the limit stop to the height of the
motorized focus.
It is easiest to move the limit stop by keeping the
screwdriver inserted and moving it upwards.
2. Unscrew the screw of the limit stop on the
side of the focusing column.
4. Tighten the screw of the limit stop.
Readjusting the motorized focus
The motorized focus is factory-adjusted and
normally does not need to be readjusted—
even if the maximum travel path is changed.
Exception: If the power fails while the
•
motorized focus is moving, the position
data are lost. In this case, the calibration must
be repeated using the Leica LAS software or the
Leica SmartTouch™. To do so, please consult the
respective manual.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
22
Optics Carrier
Tools used
Assembling the optics carrier
ρ
Hex socket screwdriver, 4 mm
1. Place the optics carrier on the focusing
column so that the screw fits into the thread
provided and the lug fits into the groove.
2. Press the optics carrier backwards to the
focusing column and screw it in place using
your other hand.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
23
Tube
All intermediate tubes that fit between the
optics carrier and the binocular tube are fitted
in the same manner.
Assembling the tube
2. Push the tube (for example, the inclined
binocular tube) into the dovetail ring and
rotate it slightly in both directions until the
positioning screw meshes with the guide
groove.
3. While holding the tube only slightly, care-
fully tighten the positioning screw. It is
automatically brought to the correct posi-
tion.
Tools used
ρ
No tools required.
Preparations
1. Unscrew the positioning screw and remove
the protective cover.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
24
Eyepieces
Tools used
Preparation
Inserting the eyepieces
ρ
No tools required.
1. If you want to use an optional graticule,
3. Push the eyepieces into the tubes as far as
they will go and check to ensure that they
fit tightly and accurately.
Magnification range
You can extend the overall magnification range
using available 10×, 16×, 25× and 40× wide-
field eyepieces for persons wearing glasses.
2. Remove the plastic tube guard.
4. Securely tighten the clamping screws.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
25
Objective
Tools used
Attaching the objective
Hold the objective firmly during assem-
bly and disassembly so that it does not
fall onto the stage plate. This applies particu-
larly to the 2x planapochromatic objective,
which is very heavy. Remove all specimens from
the stage plate first.
Alternative fastening options
ρ
ρ
No tools required.
If using the objective nosepiece, read the
•
ρ
Preparation
1. Remove the protective cap on the optics
carrier by turning it.
If using the Leica FluoCombi III™, read the
2. Screw the objective clockwise into the
optics carrier.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
26
UV Protection Screen: Assembly
Tools used
Use with one objective
ρ
Allen key
1. Adjust the UV protection screen laterally
using the arm.
Intended use
2. Unscrew the hexagon-head screw.
The UV protection screen in front of the speci-
men plane prevents the user from looking
directly into the UV rays.
3. Adjust the UV protection screen using the
arm.
Safety Notes
4. Tighten the hexagon-head screw.
UV radiation can damage the eyes.
•
Therefore, it is mandatory to install the
UV filter and adjust it correctly.
5. Fasten the UV protection screen using a
hexagon-head screw to the left or right side
of the microscope carrier.
Always position the UV protection
screen so that the operator can never
look directly at the light spot.
•
Download from Www.Somanuals.com. All Manuals Search And Download.
Leica M-series
User Manual
27
Objective Nosepiece – Assembly
Preparations
2. Rotate the moving part by 90° and attach
the objective nosepiece to the drive hous-
ing from the front (!). Screw the objective
nosepiece firmly into place.
4. Screw the optics carrier onto the objective
nosepiece.
Hold the objectives firmly during
•
assembly and disassembly so that they
do not fall onto the stage plate.
5. Screw both objectives onto the objective
nosepiece. It makes no difference which
position an objective occupies.
ρ
Move the drive housing all the way upwards
and remove the optics carrier, if the carrier
has already been installed.
6. Unscrew the locking screws on both sides
of the objective nosepiece.
Assembly
1. Remove the transport anchor from the
objective nosepiece.
3. Unscrew the three Phillips screws on the
objective mount of the optics carrier and
remove the intermediate ring.
You can now adjust the parfocality (see instruc-
tions on the next page).
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
28
Objective Nosepiece – Adjusting Parfocality
The following procedure only has to be carried
out once. Afterwards, both objectives are parfo-
cal, meaning that the specimen remains in focus
when the objective nosepiece is rotated.
Adjustment
1. Rotate the 2× objective into the beam path
and set it to the lowest magnification.
7. Toggle to the 1× objective.
8. Turn the objective on the thread in both
directions until the specimen appears
absolutely sharp.
2. Focus on the specimen.
This procedure must be repeated if you replace
either of the two objectives with another.
3. Rotate the 1× objective into the beam
path.
By means of zooming, check that the behavior
of the objective is parfocal. Repeat the check
with the other objective. If it is not parfocal,
repeat the procedure.
The following example assumes the combi-
nation of the 1× and 2× planapochromats. If
you are using another objective combination,
replace the 2× objective in the description with
the objective with the stronger magnification.
4. Turn the objective on the thread in both
directions until the specimen appears
sharp.
9. Tighten the locking screws.
5. Toggle to the 2× objective.
Preparation
6. Select the strongest magnification and
refocus until the specimen appears abso-
lutely sharp.
ρ
Open the iris diaphragm.
ρ
Set the dioptric correction of the eyepieces
to "0".
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
29
Transmitted-light Base TL ST
Unpacking the base
Focusing drive and column
1. Unscrew the extension plate from the base
using the Allen key provided.
2. Attach your focusing drive column from
below using the four hexagon-head
screws.
The base is delivered with the adapter plate
installed. Make sure the instruments are
unpacked on a flat, sufficiently dimensioned,
and non-slip surface.
3. Re-attach the adapter plate to its origi-
nal position using the six hexagon-head
screws.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
30
FluoCombi III™ – Preparations
Before assembling the FluoCombi™,
you have to disconnect all instruments
2. Screw the two screws into the holes
provided for storage.
4. Screw the other planapochromatic stereo
objective into the FluoCombi III™.
from the power supply. Failure to observe these
instructions can result in damage to the micro-
scope and the connected instruments.
Preparations
1. Remove the two screws used as transport
anchors.
In this way, you can secure the FluoCombi III™
for transport whenever necessary.
3. Screw the 5× planapochromat into the
FluoCombi III™.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
31
FluoCombi III™ – Assembly
1. Guide the FluoCombi III™ in from below,
while holding it flat. Ensure that it is aligned
with the holder on the focusing column at
the left and right.
2. Hold the FluoCombi III™ securely and
screw it to the focusing column using the
provided screw.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
32
FluoCombi III™ – Assembly (cont'd.)
Assembling the optics carrier
1. Unscrew the three screws and the adapter
ring on the optics carrier.
2. Attach the optics carrier to the FluoCombi
4. Assemble the tube (see Page 24).
III and screw it into place.
5. Assemble the illuminator (see Page 51 ff.)
3. Open the iris diaphragm completely.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
33
FluoCombi III™ – Adjusting the Parfocality
Setting up the objective nosepiece
The following configuration only has to
be carried out once. It ensures that the
parfocality remains intact, and thus the speci-
men does not need to be brought into focus
again.
1. Place a specimen under the microscope.
7. Unscrew the clamping screw on the
objective.
2. Rotate the stereo planachromatic objective
under the beam path.
3. Reduce the view so that you can see the
specimen in the overview.
4. Center the specimen under the beam path.
5. Adjust the magnification to the highest
level and focus on a flat specimen.
6. Rotate the 5× planachromat under the
beam path.
8. Reduce the view so that you can see the
specimen in the overview.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
34
FluoCombi III™ – Adjusting the Parfocality (cont'd.)
9. By turning the objective ring, focus on the
specimen until the specimen appears abso-
lutely sharp.
11. Refocus.
12. Tighten the clamping screw (see Step 7).
13. Rotate the stereo planachromatic objective
under the beam path.
This causes the two objectives to be parfo-
cal. This means that from then on, you can
toggle between the two objectives without
refocusing.
10. Enlarge the view just enough for the center
of the specimen to be visible in the field of
view.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
35
FluoCombi III™ – Centering the Objectives
The following configuration only has to
be carried out once. It ensures that the
specimen remains in the optical center when
the objective is changed.
Centering the objective
1. Rotate the 5× planachromat under the
beam path.
4. Center the objective over the specimen by
loosening or tightening the screw on one
side of the objective holder while turning
the screw on the other side in the opposite
direction.
2. Focus on the midpoint of the specimen.
To attain optimum results for the follow-
ing settings, insert a graticule into the
eyepiece. This makes locating the center vastly
easier.
3. Rotate the stereo planachromatic objective
under the beam path.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
36
FluoCombi III™ – Filter and UV Protection Screen
Inserting the dichroic mirror
1. Pull the dummy filter carrier out of the
holder.
Assembling the UV protection screen
1. Screw the UV protection screen to the
holder provided on the optics carrier.
2. Insert the corresponding dichroic mirror.
2. Orient the UV protection screen so that you
cannot look directly into the UV light.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
37
Transmitted-light Base TL BFDF: Before First Use
Removing the transport anchors
Before you can use the transmitted-
•
light base for the first time, it is abso-
lutely necessary to remove the two transport
anchors.
Anchor of mirror
Anchor of switching slide
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
38
Transmitted-light Base TL BFDF
Standard delivery
The selected stage is mounted on the base
before commissioning. You can switch between
the stages at any time with just a few hand
movements.
Standard stage
1. Take the glass plate from the rectangular
gap in the standard stage.
The base is delivered with the adapter plate
installed. The selected stage (Leica IsoPro™
mechanical stage or Leica standard stage
10 447 269), and the focusing drive will have to
be mounted later.
2. Position the stage on the transmitted-light
base in such way that the four holes align
over those in the base.
The following paragraph assumes use of the
base without the stage mounted. Disassembly
is performed in reverse order of the following
steps.
Stage assembly
The Leica TL BFDF transmitted-light base can be
equipped with three different stages.
3. Attach the stage to the base with the four
supplied hexagon-head screws.
ρ
Leica IsoPro™ manual mechanical stage
Leica IsoPro™ automatic mechanical stage
4. Insert the glass plate back into the standard
stage.
ρ
ρ
Leica standard stage 10 447 269
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
39
TL RC™ / TL RCI™
The base is delivered with the adapter plate
installed. The selected stage (Leica IsoPro™
mechanical stage or Leica standard stage
10 447 269), and the focusing drive will have to
be mounted later.
Standard stage
1. Take the glass plate from the rectangular
gap in the standard stage.
2. Position the stage on the transmitted-light
base in such way that the four holes align
over those in the base.
Ensure that the instruments are unpacked on a
level, adequately sized, and nonskid underlay.
3. Attach the stage to the base with the four
supplied hexagon-head screws.
Stage assembly
The Leica TL RC™/ RCI™ transmitted-light base
can be equipped with three different Leica
stages. The selected stage is mounted on the
base before commissioning. You can switch
between the stages at any time with just a few
hand movements.
4. Insert the glass plate back into the standard
stage.
The following paragraph assumes use of the
base without the stage mounted. Disassembly
is performed in reverse order of the following
steps.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
40
IsoPro™ Manual Mechanical Stage: Assembly
Leica IsoPro™ Mechanical Stage
Before the Leica IsoPro™ mechanical stage is
Left or right operation
Before the Leica IsoPro™ mechanical stage is
mounted to the base, the axis containing the
control buttons is attached either on the left or
the right side of the mechanical stage.
mounted to the base, the axis containing the
control buttons is attached either on the left or
the right side of the mechanical stage.
If the controls are to be mounted on the left-
hand side, the gear rod on the bottom side of
the mechanical stage must be unscrewed and
reattached in reverse.
If the controls are to be mounted on the left-
hand side, the gear rod on the bottom side of
the mechanical stage must be unscrewed and
reattached in reverse.
1. Take the glass plate from the mechanical
stage.
2. Turn the mechanical stage around and
place it onto a non-slip surface.
3. Change the gear rod from the left to the
right-hand side.
4. Skip the next two steps to install the
controls.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
41
Leica IsoPro™ Manual Mechanical Stage: Assembly (cont'd.)
1. Take the glass plate from the mechanical
stage and turn it around.
Control assembly
1. Take the glass plate from the mechanical
stage and turn it around.
Mechanical stage assembly
1. Place the mechanical stage onto the base.
2. Change the gear rod from the left to the
right-hand side.
2. Pull the upper part of the mechanical stage
carefully towards the user, fixing the lower
part onto the transmitted-light base.
2. Attach the axis with the control buttons to
the desired side. The fastener snaps into the
mechanical stage magnetically.
4. Attach the axis with the two supplied hexa-
gon-head screws.
5. Attach the cover rail to the mechanical
stage.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
42
Leica IsoPro™ Manual Mechanical Stage: Assembly (cont'd.)
3. Attach the mechanical stage evenly to the
three threaded holes.
Focusing drive and column
1. Unscrew the extension plate from the base
using the Allen key provided.
4. Now move the mechanical stage as far as it
will go in the direction of the column.
2. Attach your focusing drive column from
below using the three hexagon-head
screws.
5. Insert the glass plate into the mechanical
stage.
3. Re-attach the adapter plate to its original
position using the three hexagon-head
screws.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
43
Leica IsoPro™ Motorized Mechanical Stage: Assembly
Basics
1. Unpack the mechanical stage from the
transport packaging and position it on the
transmitted-light base.
2. Secure the motorized mechanical stage to
the base using three M4 screws.
The transmitted light bases of the LeicaTL series
(TL BFDF, TL RC™, TL RCI™) are supplied with an
installed extension plate. The selected stage
(Leica IsoPro™ mechanical stage or standard
stage 10 447 269) and the focusing drive will
have to be mounted later.
1
The motorized mechanical stage is a sensi-
tive precision instrument. During installation,
avoid subjecting the stage to impact or severe
vibrations.
2
1
2
Microscope base
Motorized mechanical stage
3× M4 screws
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
44
Leica IsoPro™ Motorized Mechanical Stage: Assembly (cont'd.)
3. Remove the two M3 screws and the holder
from the mechanical stage.
4. Remove the two M4 screws and the sleeve
from the mechanical stage.
5. Remove the four shock-absorbing cartons
from the mechanical stage.
After removal, keep all the transport
anchors in the plastic bag provided for
future transport.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
45
Leica IsoPro™ Motorized Mechanical Stage: Assembly (cont'd.)
The bases
Mechanical stage and base
Never move the sledge of the motor-
ized mechanical stage manually in the
X direction, as otherwise the mechanical system
will be damaged!
The TL BFDF, TL RC™ and RCI™ transmitted-light
bases can be equipped with three different
stages: Standard stage, manual and automated
IsoPro™ mechanical stage. The selected stage is
mounted on the base before commissioning.
You can switch between the stages at any time
with just a few hand movements.
•
1. Place the mechanical stage onto the base.
2. Pull the upper part of the mechanical stage
carefully towards the user and fasten the
lower part onto the transmitted-light base.
The following paragraph assumes use of the
base without the stage mounted. Disassembly
is performed in reverse order of the following
steps.
3. Attach the mechanical stage evenly to the
three threaded holes.
4. Now move the mechanical stage as far as it
will go in the direction of the column.
5. Insert the glass plate into the mechanical
stage.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
46
Leica IsoPro™ Motorized Mechanical Stage: Assembly (cont'd.)
Mechanical stage for X-Y stage control
module
1. Plug the CTL2 plug of the mechanical stage
motor into one of the three available CTL2
interfaces.
As soon as all instruments have been plugged
into the control box and it has been plugged
into the power supply, the mechanical stage
initializes and automatically drives to the mid
position.
2. Plug the CTL2 plug of the Leica PSC control-
ler into another CTL2 interface.
4. Connect the PC (where applicable) and
X/Y Stage DCI Module (using a suitable
USB cable).
ρ
The third CTL2 interface is available for
connecting other instruments, such as the
Leica foot switch (10 447 398) or TL RCI™
transmitted light base (10 446 352).
ρ
The 15-pin Sub-D interface is intended for
use with the Leica SmartMove™ controller
(11 501 197).
3. Plug a power cable into the socket provided
and into a grounded power socket.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
47
Cables: Connections
The new Leica M-series features extensive
encoding with which various microscope data
and settings can be read out, transferred to the
PC and reproduced later.
The terminals
The connection to the PC and to other instru-
ments is made using the terminals on the rear
side of the column:
1. 2 CTL2 terminals for auxiliary equipment
such as the ring illuminator, the base and
other accessories from the Leica product
range.
1
ρ
The interface to the optics carrier is on the
column.
3
2
2. Terminal for the 50-watt power supply
provided.
3. USB terminal for the connection to the PC.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
48
Cables: Cable Duct
The integrated cable duct in the column enables
a neat cable layout around the microscope.
For example, the USB or FireWire cables of the
camera can be stowed in the cable duct.
Feeding the cables
1. Unscrew the three screws on the cable
duct.
3. Place the cables in the cable duct and screw
the cover on tightly.
2. Remove the cover of the cable duct.
Tip: Estimate the length of the cable ends you
will need before screwing on the cover. For
thick cables, it is difficult to change the length
retroactively.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
49
Cables: Diagram
Alternative installation
Please use the Leica Application
Suite to configure the functions
of the system.
LED5000 RL
LED5000 MCI™
DCI Controlbox
TL RCI™
PC USB
Power/USB
115/230 V
Footswitch(es)
Smart Move
PSC
SmartTouch
Cross Stage
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
50
Leica LED5000 MCI™
The Leica LED5000 MCI™ (for "Multi Contrast
Illumination") is installed using two screws. For
optimum accessibility, the optics carrier should
be removed during assembly (see Page 23.)
Assembly
1. Hold the LED5000 MCI™ with one
hand and tightly screw the retain-
ing stirrups on both top holes on the drive
housing.
2. Connect the CAN-bus cable provided to
either of the two sockets. (The flat part of
the plug must be facing downwards.)
•
3. Plug the other end of the cable into one of
the two "CTL2" sockets on the column.
Constraints
The Leica LED5000 MCI™ cannot be used
together with the objective nosepiece.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
51
Leica LED5000 MCI™: Alternative Assembly
Under certain circumstances, the light source
must not be moved along with the optics
carrier. A typical example is multifocus images
in which the Z-stack changes while the angle
of incidence of the light must remain the same.
For such purposes, the Leica LED5000 MCI™ is
directly fastened to the column.
2. Screw the retaining stirrup into the column
3. Push the Leica LED5000 MCI™ onto the
retaining stirrup.
at the notch using the single screw. If you
tighten the screw, the retaining stirrup
is automatically moved into the correct
position.
Installation on the column
1. Pull the retaining stirrup out of the Leica
LED5000 MCI™.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
52
Leica LED5000 RL
The ring illuminator, the Leica LED5000 RL
("Ring Light"), is installed on the objective using
a single screw. It has been optimized for a work-
ing distance between 60 and 70 mm.
Assembly
3. Plug the other end of the cable into one of
the two "CTL2" sockets on the column.
1. Connect the CAN-bus cable to the ring illu-
minator. The flat part of the plug must be
facing upwards.
For optimum accessibility of the specimen,
the ring illuminator should be installed with
the cable facing backwards. However, it is
also possible to turn the ring illuminator side-
ways, for example if simultaneously using the
Leica LED5000 MCI™ system illumination. In this
case, the ring illuminator cannot be connected
directly to the Leica LED5000 MCI™.
2. Push the ring illuminator over the objective
as far as it will go and screw it into place.
Constraints
The Leica LED5000 RL can be used in conjunc-
tion with the planapochromat 1× and planapo-
chromat 0.63× objectives. With all other
objectives, the working distance is too low for
adequate illumination.
The ring illuminator cannot be used together
with the objective nosepiece.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
53
Leica EL6000 – Safety Notes
The manufacturer assumes no respon-
sibility or liability for any use outside of
the intended use or use outside of the specifica-
tions from Leica Microsystems Wetzlar GmbH or
any risks resulting from such use.
General safety notes
This safety class 1 device was built and tested
in accordance with
EN 61010-2-101:2002
EN 61010-1:2001,
IEC 1010-1:2001,
Safety requirements for electrical equipment
for measurement, control and laboratory use.
Unauthorized alterations to the instru-
ment or noncompliant use shall void all
rights to any warranty claims.
•
•
If any safety defects or malfunctions
of the Leica EL6000 are identified, the
•
In such cases, the Declaration of Conformity
shall be invalid.
instrument must be immediately disconnected
from the power system and secured against
additional use. For repair, the Leica EL6000
must be sent to the supplier or an authorized
representative of the supplier.
The devices and accessories described in this
operating manual have been tested for safety
and potential hazards.
This (IVD) device is not intended for use
in the patient environment defined by
•
DIN VDE 0100-710. It is also not intended to
be combined with medical devices as defined
by EN 60601-1. If a microscope is electrically
connected to a medical instrument in accor-
dance with EN 60601-1, the requirements
defined in EN 60601-1-1 shall apply.
The responsible Leica affiliate or the main plant
in Wetzlar must be consulted whenever the
device is altered, modified or used in conjunc-
tion with non-Leica components that are
outside of the scope of this manual.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
54
Leica EL6000 – Safety Notes (cont'd.)
The Leica EL6000 compact light source
generates high-energy light with invisi-
The interior of the lamp inserted into the
unit contains highly toxic substances.
Only fuses of the specified type and
the listed rated current may be used as
replacements. The use of incorrect fuses may
result in a fire hazard.
•
•
•
ble components. There is a risk of being dazzled
or blinded by light! Never look directly into the
light guide output of the instrument or into
the output of the light guide connected to the
instrument. Always ensure that the output of
the connected light guide is securely connected
to the system to be illuminated before switch-
ing on the compact light source. Furthermore,
before switching on the compact light source,
dim the light output all the way using the
intensity switch (left limit stop of the switch) to
prevent damage to the connected system.
At the end of its service life, dispose of the lamp
according to applicable regulations. The manu-
facturer of the lamp provides corresponding
information, for example in the lamp's shipping
documents. Follow the manufacturer's instruc-
tions in case the lamp bursts.
The instrument is intended exclusively
for operation in dry rooms. Do not
use the instrument in rooms with explosion
hazard.
•
The Leica EL6000 compact light source
is designed for a voltage range of
•
100 – 240 VAC, 50 – 60 Hz. Within this voltage
and frequency range, the instrument always
adapts to the connected power supply. Operat-
ing the instrument with a power supply voltage
outside this range can destroy the instrument
and the connected components!
To prevent damage to the light guide
connected to the device, the heat-
•
absorbing filter inserted into the device must
be free of damage (gaps, cracks etc.). If the
heat-absorbing filter is not inserted or is defec-
tive, the service life of the light guide will be
decreased.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
55
Leica EL6000 – Safety Notes (cont'd.)
Electrical safety
For indoor use only.
Supply voltage
Frequency
100–240 V~ ( 10%)
50–60 Hz
Power consumption
Fuses
Max. 210 VA
5×20, 2.5 A, slow-blowing, breaking capacity H, see Page 113
Ambient temperature
Relative humidity
Overvoltage category
Pollution degree
0 – 40 °C
10%–90%
II
2
Download from Www.Somanuals.com. All Manuals Search And Download.
Leica M-series
User Manual
56
Leica EL6000 – Preparations
Setting up the instrument
The lamp used in the instrument
becomes very hot during operation and
has high internal pressure when hot. Before
changing the lamp, it is mandatory to allow the
instrument to cool off for at least 20 minutes.
Inserting the lamp
Set up the Leica EL6000 so that the front
•
1. Pull the power plug out of the socket, so
that the EL6000 is disconnected from the
power system.
•
is readily accessible and visible.
ρ
The ventilation slots on the sides and back
2. Unscrew both screws of the cover using a
3 mm Allen key.
panel of the device must not be covered.
The Leica EL6000 compact light source
ρ
Maintain a free room of at least 150 mm on
the rear panel of the instrument.
•
comes with a factory-supplied lamp,
but the lamp is not installed when shipped. This
minimizes the danger of damage to the lamp
during transport.
Instructions for lamp replacement
The lamp contains mercury! Be abso-
Before transporting the Leica EL6000
•
lutely certain to follow the handling
instructions and safety notes provided with the
lamp.
•
compact light source, you first have to
remove the lamp.
3. Remove the housing cover.
Maintain cleanliness when inserting the
•
lamp. Remove any clinging packaging
materials.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
57
Leica EL6000 – Preparation (cont'd.)
4. Carefully lay the Leica EL6000 on its side so
that the opening faces you.
5. Pull the pressure bolt back towards the
front plate using the lever.
Ensure that the groove in the contact surface of
the lamp matches up with the corresponding
plug in the lamp mount.
This makes it easier to insert the lamp.
6. Insert the lamp.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
58
Leica EL6000 – Connection to the Fluorescence Lamp Housing
Always connect the light guide to the
lamp housing of the stereomicroscope
in order to prevent danger to the user from the
high-energy light.
2. Insert the adapter into the fluorescence
lamp housing and retighten the locking
screw.
4. Insert the short end of the light guide into
the lamp housing of the stereomicroscope
as far as it will go.
•
5. Securely tighten the clamping screw.
1. Unscrew the positioning screw and remove
the protective cover of the fluorescence
lamp housing.
3. Unscrew the three red protective caps on
the light guide.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
59
Leica EL6000 – Preparation (cont'd.)
7. When the lamp makes full contact with the
lamp mount, release the lever.
9. Check to make certain that a heat-absorb-
ing filter has been inserted into the shaft in
order to protect the connected light guide.
8. Connect the plug of the lamp with the
coupling in the instrument.
Ensure that no wires are touching the reflector
of the lamp.
10. Close the housing cover and insert the
screws.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
60
Leica EL6000 – Replacing the Lamp
The procedure for replacing the lamp does not
differ from the installation procedure outlined
on Page 57, except for the fact that the existing
lamp has to be removed first.
3. Remove the housing cover.
4. Carefully lay the Leica EL6000 on its side so
that the opening faces you.
Replacing the lamp
This makes it easier to insert the lamp.
The lamp used in the instrument
becomes very hot during operation and
has high internal pressure when hot. Before
changing the lamp, it is mandatory to allow the
instrument to cool off for at least 20 minutes.
5. Pull the pressure bolt back towards the
front plate using the lever.
6. Pull out the lamp and disconnect the plug
from the coupling in the instrument.
1. Pull the power plug out of the socket, so
that the EL6000 is disconnected from the
power system.
7. Install the new lamp as outlined on Page 58,
beginning with item 6.
2. Unscrew both screws of the cover using a
3 mm Allen key.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
61
Leica EL6000 – Connection to the Fluorescence Lamp Housing (cont'd.)
6. Insert the long end of the light guide into
the light output of the Leica EL6000. There
must be a noticeable click.
Only use a light guide with a light input
of the type "Storz long", as otherwise
damage to the instrument and danger to the
user can result (risk of being dazzled).
•
Before you open the shutter, the light
•
guide must be connected on both
sides. Otherwise, the escaping light can cause
injury to eyes and skin and damage to furni-
ture. Never look into the light escaping from
the light guide!
5. Connect the Leica LA6000 to the power
system using the power cable.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
62
The Fastest Route to Success
Your Leica stereomicroscope has been delivered in completely assembled
condition by your Leica partner, and naturally you want to get right to
work. Therefore, your next step should be to study the Quick Start Guide,
which outlines the most important steps at a glance.
This manual will then familiarize you with the finer details of your micro-
scope. The following pages contain important, practical information that
makes using it every day easier.
Take time to read it – it's worth it!
The M series Quick Start Guide (included with instrument).
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
64
Overview of an M Series Microscope
1
Eyepieces for spectacle wearers with dioptric correction
and eyecups
2
3
4
5
6
7
8
9
Trinocular tube
Fluorescence housing
Filter Changer
Display
Motorized Focus
Objective Nosepiece
Leica SmartTouch
UV protection screen
1
2
3
4
5
6
7
9
8
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
65
The Correct Interpupillary Distance
The interpupillary distance is correctly set if you
see a single circular image field when looking
at a specimen.
Adjusting the interpupillary distance
1. Look into the eyepieces.
3. Slowly approach the eyepieces with your
eyes until you can see the complete image
field without corner cutting.
2. Hold the eyepieces with both hands. Push
the eyepieces together or separate them
until you see a circular image.
If you are still a novice microscope user, you
may need a short time to become accustomed
to this. Not to worry—after a little while, it will
become automatic.
Reference value
The distance between eye and eyepiece
measures approx. 22 mm for 10/23B wide-field
eyepieces for persons wearing glasses.
✓
✗
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
66
Using the Eyepieces
The eyepieces form the connection between
the tube and the eye of the observer. Simply
push them into the tube and they are ready to
use.
If you do not wear glasses:
1. Hold the eyepiece firmly and rotate the
eyecups forwards counterclockwise.
If you wear glasses:
1. Hold the eyepiece firmly and rotate the
eyecups clockwise towards the rear, as
otherwise the viewing distance is too
great.
2. If an eyepiece is equipped with the inte-
Each eyepiece offers a certain magnification
factor that has a determinative effect on the
total magnification. Furthermore, all Leica
eyepieces can be equipped with practical grati-
cules that enable measuring and quantifying of
specimens.
Dioptric correction
A built-in dioptric correction is available for
eyeglass wearers. For more information, refer
to Page 77.
grated dioptric correction, turn the value to
the "0" mark.
2. If an eyepiece is equipped with the inte-
grated dioptric correction, turn the value to
the "0" mark.
By the way, one benefit of viewing with
eyeglasses is a drastically lower risk of bacte-
rial transmission (see Page 76). The soft mate-
rial of the eyecup also ensures that your glasses
will not be scratched, even if they contact the
eyepiece.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
67
Focusing
Focusing raises or lowers the stereomicroscope
using the focusing drive. The specimen detail is
brought into sharp focus as soon as it is in the
focal point of the objective.
Focusing
Coarse/fine adjustment
ρ
The inner, coarse adjustment is used for
The sharpness is adjusted using the coarse/fine
adjustment. The resolution of the coarse/fine
adjustment is 1 µm.
covering great distances.
ρ
The outer, fine adjustment is used for fine
focusing.
ρ
The focusing drive can be operated either
2. The coarse/fine adjustment carries a
left- or right-handed.
•
load of up to 15 kg.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
68
Adjusting the Resistance of the Focus Drive
Adjusting the resistance
Is the focus movement too loose or too tight?
Does the outfit tend to slide downwards? The
resistance can be adjusted individually depend-
ing on the equipment weight and personal
preferences as follows:
1. Grip the outer drive knobs with both hands
and turn them towards each other until
the desired resistance is reached during
focusing.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
69
Changing Magnification (Zoom)
All M series microscopes have an integrated
zoom. The name indicates the zoom range
covered:
Zooming
1. Look into the eyepieces.
2. Focus on the specimen.
ρ
Leica M165 C = 16.5:1
ρ
Leica M205 C = 20.5:1
3. Rotate the magnification changer until the
desired magnification is configured.
The rotary knob for the zoom can be used either
left or right-handed.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
70
Ratchet Steps and Magnification Levels
The zoom button can optionally be operated
either with or without ratchet steps. Continu-
ous zoom is possible when the ratchet steps are
disabled, which many users find convenient.
On the other hand, when the ratchet steps are
enabled, photographs, measurement results
etc. can be reproduced more accurately.
Enabling and disabling ratchet steps
1. Push the top button downwards to disable
the ratchet steps.
Magnifications and fields of view
information about the magnifications and field
of view diameters, with consideration given to
the position of the magnification changer and
the eyepiece and objective combination used.
2. Push the bottom button upwards to enable
the ratchet steps.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
71
Parfocality: More Comfort and Convenience for Your Work
All Leica stereomicroscopes are parfocally
matched, meaning that you can view a focused
specimen from the lowest to the highest
magnification without having to refocus. The
focus needs only be readjusted if you want to
see a specimen location that is located higher
or lower.
Parfocality
1. Enlarge the view to the maximum level.
2. Focus on the specimen.
You are done! Even if you select a smaller work-
ing distance, the specimen remains pin-sharp.
Requirements for parfocal work
The parfocality is maintained until you focus on
another level of the specimen.
ρ
If you are using an eyepiece with dioptric
correction, the procedure differs from this
description. For more information, refer to
Page 78.
ρ
For the procedure for adjusting the parfo-
cality for the objective nosepiece, refer to
Page 29.
ρ
For the procedure for adjusting the parfo-
cality for the Leica FluoCombi III™, refer to
Page 34.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
72
Iris Diaphragm
The iris diaphragm in the optics carrier of your
M series microscope fulfills the same purpose
as the iris diaphragm in a camera: it regulates
the available light, which changes the depth
of field. The "depth of field" (or "focus depth")
is the area of a specimen that is brought into
sharp focus.
Closing the iris diaphragm
Opening the Iris Diaphragm
ρ
ρ
Close the iris diaphragm by turning the
Open the iris diaphragm by turning the
knob to the left. The subject appears darker
and the depth of field increases.
knob to the right. The subject now appears
brighter, but the depth of field decreases.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
73
Magnification Factors of the Eyepieces
An eyepiece not only makes it possible to look passively into the micro-
scope, but also has a critical effect on the maximum magnification. The
magnification factor is between 10x and 40x.
The following eyepieces are available for the M series:
Magnification
Dioptric correction
5 diopter settings
5 diopter settings
5 diopter settings
5 diopter settings
Order number
10 450 023
10 450 024
10 450 025
10 450 026
10×
16×
25×
40×
Download from Www.Somanuals.com. All Manuals Search And Download.
Leica M-series
User Manual
75
Health Notes
Potential sources of infection
Direct contact with eyepieces is
a
•
potential transmission method for
bacterial and viral infections of the eye. The risk
can be kept to a minimum by using individual
eyepieces or detachable eyecups. Eyecups can
be ordered separately. Please contact your
Leica partner.
Separate eyecups are an effective
way of preventing infections.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
76
Dioptric Correction
All Leica eyepieces are also available with built-
in dioptric correction, allowing the microscope
to be used without glasses even by those with
Using the Dioptric Correction
1. Set the dioptric correction of both eyepieces
to the mid position ("0" diopter settings).
Note that when using dioptric correction,
the advantage of parfocality is lost—thus
you have to manually refocus each time you
change the zoom level. To also use parfocality
with dioptric correction, refer to the instruc-
vision problems. The correction comprises
diopter settings.
5
2. While wearing your glasses, look through
the eyepieces and focus on the specimen.
3. Rotate both eyepieces to the maximum
value of "+5".
4. Hold one eye closed and rotate the other
eyepiece in the "-" direction until the speci-
men appears sharp.
5. Then, open the other eye and correct the
diopter settings until the image is uniformly
sharp.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
77
Dioptric Correction and Parfocality
Leicastereomicroscopesareparfocallymatched.
The prerequisite for this is the correct setting of
the diopters and the parfocality. The following
adjustments only have to be carried out once
by each user.
Adjusting
7. Look into the eyepieces.
1. Set the dioptric correction for both
eyepieces to "0".
8. Slowly rotate each eyepiece individually
in the "–" direction until each eye sees the
object sharply imaged.
2. Select the lowest magnification and focus
on a flat specimen.
9. Select the highest magnification and
refocus if necessary.
Preparations
3. Select the highest magnification and read-
just the sharpness.
ρ
Move the lever of the video/phototube to
the "observation" position and open the
diaphragm.
Now, if you adjust the magnification from the
lowest to the highest level, the specimen is
always brought into sharp focus. If not, repeat
the process.
4. Select the lowest magnification again, but
do not look into the eyepieces.
ρ
If you are using the microscope carrier AX,
set it to stereoscopic observation.
6. Rotate the eyepieces counterclockwise
in the "+" direction as far as they will go
(+5 diopter settings).
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
78
Graticules
Use
2. Clamp the graticule on the insert, applying
moderate pressure. Ensure that the grati-
cule fits tightly.
4. You can now align the graticule by rotating
the eyepiece in the tube and then tighten-
ing it using the clamping screw.
Leica graticules make length measurements
and counting easier, particularly for work-
stations that are not equipped with a digital
camera and LAS software.
The Leica graticules for length measurements
and numbering are fitted in mounts and are
inserted into the eyepieces.
1. Screw the insert off of the eyepiece.
3. Screw the insert and graticule firmly into
place and replace the eyepiece in the tube.
Use with the Leica AX carrier
If possible, measure with the micro-
scope carrier AX in vertical position. The
measurements are more accurate without the
convergence angle in the stereoscopic image.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
79
Photography & Video
For most microscope users, digital documen-
tation has become an invaluable part of their
work. Research results can be presented in an
attractive manner; measurements on the digital
image provide clarity and, in conjunction with
the motorized IsoPro™ mechanical stage, even
images of large specimens can be captured
step by step and automatically joined to create
a new complete image.
Leica DFC cameras
Leica Application Suite
However, if you require absolute control
over the camera and need the capability for
measurement, evaluation and more in addition
to photography, the digital Leica DFC cameras
are exactly right for you. Together with the
Leica Application Suite, they provide virtually
limitless freedom of use. For additional informa-
tion about Leica cameras, refer to the camera's
documentation.
The "Leica Application Suite", or "LAS" for short,
is, as it were, the digital extension of the Leica
M series microscopes. In addition to capturing
images, it lets you control the microscope, illu-
minator, stages, cameras and more. For addi-
tional information, refer to the LAS online help.
Adapter
If camera control using the Leica Application
Suite is not required, conventional mirror reflex
and rangefinder cameras from third-party
manufacturers can be used. For this purpose,
Leica Microsystems offers a variety of adapters
that can be used together with the 50% and
100% trinocular tubes.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
81
Photo Tubes and C-mounts
Application
If there is unwanted shading at the corners even
with a compatible C-mount adapter installed, it
can be corrected using the "Shading function"
of the camera software.
Cameras from third-party suppliers
All Leica DFC cameras are equipped with a
standardized C-mount interface. In turn, the
C-mount adapter for the respective trinocular
tube is connected to this interface. This adapter
creates a solid mechanical connection between
the microscope and camera and ensures opti-
mum rendering of the microscopic image on
the image sensor of the camera.
In addition to Leica DFC cameras with the stan-
dardized C-mount interfaces, you can connect
third-party cameras to the microscope using
a T2 bayonet adapter. To do so, instead of the
C-mount adapter, simply use the correspond-
ing SLR adapter with T2 connection. However,
these third-party cameras are not integrated
into the Leica Application Suite and have to
be operated using the corresponding software
from the camera manufacturer.
Alternatively, you can also use a C-mount
adapter with higher magnification. This primar-
ily avoids the critical border area of the field of
vision and concentrates on the center of the
field of vision.
Usually, the ideal is for the digital camera to
capture as much of the field of vision as possi-
ble, while excluding as much of the black edge
of the field of vision as possible. To do so, the
magnification factor of the C-mount adapter
must match the image format of the sensor as
closely as possible (see table).
Camera
optimal
(large
suitable
(smaller
The Leica digital cameras are detailed in a sepa-
rate user manual along with instructions for
their assembly and use.
image field)
image field)
DFC290
DFC420
DFC490
DFC500
0.5×
0.5×
0.63×
0.63×
0.8×
0.63×
0.63×
0.8×
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
82
Trinocular Video/Phototube 50%
Use
With its third beam path, the trinocular video/
phototube 50% enables you to simultaneously
view and photograph a specimen. The available
light is divided as follows:
ρ
50% is available for the two eyepieces.
ρ
50% of the light is diverted to the video/
photo beam path.
Assembly
Fasten the "trinocular tube 50%" to the optics
carrier instead of the binocular observation
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
83
Trinocular Video/Phototube 100%
Use
Switchover
ρ
With its third beam path, the trinocular video/
phototube 100% enables you to either view or
photograph a specimen. This means that 100%
of the light is available to one or the other beam
path. The other beam path remains opaque or
black.
Turn the controller on the right side of the
tube into the horizontal position in order to
guide all available light into the eyepieces.
You can now observe the specimen.
ρ
Turn the controller on the right side of the
tube into the vertical position in order to
guide all available light into the camera.
You can now photograph the specimen.
Assembly
Fasten the "trinocular tube 100%" to the optics
carrier instead of the binocular observation
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
84
The Objective Nosepiece
Use
Parfocal work
Technical constraints
The objective nosepiece cannot be
used together with the LED5000 MCI™
system illumination, as the objective nosepiece
can no longer be rotated.
The objective nosepiece enables you to switch
between two objectives using just one hand
movement, for example between a 1× and a 2×
planapochromat.
With the new M series objectives, the parfo-
cality is maintained even when objectives are
changed, meaning that the specimen remains
in sharp focus during the change.
•
Older Leica objectives can continue to be
used, but without parfocality during the
objective change.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
86
The Different Types of Objectives
To meet the various requirements regarding
imaging properties, there is a choice of high-
quality interchangeable planachromatic and
planapochromatic objectives and also lower-
priced interchangeable achromatic objectives.
Achromatic objectives
Achromatic objectives with a long focal
length
For special applications, achromatic objectives
with long working distances and focal lengths
of f=100 mm to 400 mm are available.
The 0.32×, 0.5×, 0.63×, 0.8×, 1×, 1.5×, 2×
achromatic objectives offer countless variants
for selecting the object field diameter, magni-
fication ranges and working distances (see
Page 147).
ρ
Achromatic objectives are particularly
suited for specimens with high-contrast
structures.
Planachromatic objective 1×
For the highest requirements for overall image
quality, we recommend equipping the micro-
scope with the 1× plan (flat-field) objective,
which returns sharp, contrast-rich object fields.
ρ
Flat-field (planachromatic) objectives are
particularly well suited for studying flat
objects such as wafers and thin sections.
ρ
With planapochromatic objectives, the
finest structures are visible with high
contrast. The sophisticated apochromatic
correction allows these objectives to attain
the highest color brilliance and fidelity.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
88
Leica TL ST Transmitted-light Base: Controls
1
2
1
2
3
3
4
Extension plate of the transmitted-light base TL ST
Rear side of the transmitted-light base TL ST
1
2
3
Screws for changing the halogen lamp
Power connection socket
Power switch
1
Adapter plate for easy assembly of focusing
drives
2
3
4
Removable glass plate
Controller for light intensity
Adjustment for deflection mirror
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
90
Leica TL ST Transmitted-light Base: Operation
Light intensity control
Transmitted-light control
Bright field
The left control adjusts the intensity of the
12V/20W halogen illumination.
The transmitted-light base TL BFDF has a slider
that automatically moves the deflection mirror
in the base when moved. The mirror is kept in
the correct position at all times and permits
smooth changeover between bright field and
opaque transmitted light.
Bright field is suitable for examining translu-
cent objects featuring contrasting structures.
The object is directly illuminated from below
and is seen in its natural colors against a bright
background.
1. Switch on the illumination of the base at
the power switch.
ρ
2. Focus on the specimen.
Move the slider backwards until the desired
effect is achieved.
3. Set the illumination to the desired intensity
using the left control.
Inclined transmitted light
Transmitted light that traverses the object
obliquely will provide additional resolution and
information when observing semitransparent,
opaque objects.
ρ
Slowly pull the slider towards yourself until
the desired effect is achieved.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
91
Leica TL ST Transmitted-light Base: Changing Bulbs
Changing the halogen lamp
Before you change the lamp, it is abso-
lutely necessary to unplug the power
plug from the base to prevent the risk of electric
shock!
Changing bulbs
Precautionary measures
When inserting the lamp, ensure that
the cables are inside the two metal
clamps. This prevents the cables from getting
caught during insertion.
1. Unscrew the two screws on the heat sink
and pull the heat sink out, along with the
lamp.
•
The halogen lamp becomes very hot
during operation. Therefore, to avoid
being burned, let the base cool off for approx.
10 minutes after switching it off!
Do not touch new halogen lamps with
•
your bare fingers—this drastically
reduces the service life of the lamp!
2. Carefully pull out the lamp and mount by
pulling them upwards.
3. Disconnect the lamp from the mount.
4. Insert the new lamp into the mount and
reinsert the lamp holder.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
92
Leica TL BFDF Transmitted-light Base: Controls
1
2
Adapter at the focusing drive
3
Extension plate of the Transmitted-light base TL BFDF
1
Adapter plate for easy assembly of focusing
drives
2
3
Standard stage 10 447 269
Button to toggle between bright field and
dark field
Connector for cold light sources
(light conductor active f = 10mm,
end tube f = 13mm)
Button to toggle between bright field and dark field
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
93
Leica TL BFDF Transmitted-light Base: Operation
Light intensity control
Observe the user manual—in particu-
lar, all safety regulations—from the
manufacturers of the light guide and cold light
source.
Bright field
Dark field
Bright field is suitable for examining translu-
cent objects featuring contrasting structures.
The object is directly illuminated from below
and is seen in its natural colors against a bright
background.
In dark-field illumination, a ring illuminator is
used in such a way that the direct light does
not reach the objective without a specimen.
Only the structure of semitransparent, opaque
objects disperses the light, making the object
visible against a dark background.
•
ρ
Switch on the cold light source according to
the manufacturer's user manual and adjust
the brightness.
ρ
Turn the control as far as it will go towards
ρ
"BF" ("bright field").
Turn the control as far as it will go towards
"DF" ("dark field").
Transmitted-light control
The Leica TL BFDF transmitted-light base has
a control that switches the light from "bright
field" to "dark field".
Fingertip with bright field illumination
Identical subject with dark field illumination
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
94
Leica TL RC™ / TL RCI™: Controls
1
2
1
2
3
4
5
6
7
3 4 5
6
Vertical column - transmitted-light base
adapter plate
1
2
3
4
5
6
Power switch
Power connection socket
USB socket, type B
USB socket, type A
2 CAN bus
1
Heat sink of the integrated halogen illumina-
tion (only TL RCI™)
2
3
4
5
Extension plate for focusing drives
Standard stage 10 447 269
Filter holder
Control of top and bottom flaps of the
Rottermann Contrast™
Screws for changing the halogen lamp
6
7
Button for mirror and horizontal movement
of the mirror
Transmitted-light base
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
95
Leica TL RCI™: The Deflection Mirror
Tilted mirror
Functions of the rotary knob
The built-in mirror features one flat and one
concave side and can be rotated and moved.
The concave side has been specially designed
for the optical requirements of objectives with a
high numerical aperture. The black rotary knob
on the left side of the transmitted-light base
can be used to rotate the built-in path-folding
mirror and move it forwards/back.
The rotary knob (1) fulfills the following tasks:
ρ
Turning the deflection mirror from the flat
to the concave side
ρ
Slight tilting to guide the light beam
through the specimen plane at a steeper or
flatter angle
1
ρ
The concave cutout on the handle indicates the
concave side of the mirror, allowing intuitive
operation at any time without eye contact.
Moving the deflection mirror (forwards/
back)
Inverted world?
Depending on the properties of the specimen
(refractive index of the environment) and the
perception of the viewer, it is possible that
the switches described below for positive and
inverted relief contrast are to be operated
in reverse. This means that the lower switch,
rather than the upper switch, controls inverted
the relief contrast and vice-versa.
The angle of light incidence in the specimen
plane changes depending on the tilt and posi-
tion of the mirror. As a result, switching between
transmitted light bright field, oblique illumina-
tion and dark field-like illumination is possible.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
96
Leica TL RCI™: Color Intensity and Temperature
The transmitted-light base TL RCI™ has two
electronic potentiometers that control the color
intensity (1) and color temperature (2).
Using a USB mouse (only TL RCI™)
The Leica USB mouse controls the CCIC™ and
dimming function of the TL RCI™ base. Connect
the mouse to the corresponding USB port of
the base.
The controller for the color temperature simul-
taneously serves as an electronic shutter:
ρ
The scroll wheel of the mouse is occupied
ρ
To interrupt your work, click the potentiom-
eter (2).
by default with the CCIC™ control system
and is used to control the illumination
intensity.
1
2
ρ
Click again to switch on the illumination.
ρ
The electronics returns the color tempera-
ture to the previously configured settings.
To switch the illumination on or off, briefly
click the scroll wheel.
1
2
Potentiometer for controlling the CCIC™
(Constant Color Intensity Control) illumina-
tion intensity
Potentiometer for controlling the color
temperature
ρ
To enter or exit dimming mode, click and
hold down the scroll wheel for longer than
2 seconds.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
97
Leica TL RC™ / TL RCI™: Operation
Setting the relief contrast
Depending on the flap position, a part of
Increased contrast without relief is attained
if both diaphragms are set to 45°. A gap-like
illuminated area is created. By tilting the deflec-
tion mirror slightly, you can move the gap over
the entire field of view and quickly toggle
between positive and negative relief images.
The dynamic effect makes it easy to distinguish
phase structures from amplitude structures.
The two switches on the left side of the trans-
mitted-light base TL RC™/TL RCI™ activate two
built-in flaps. The outer switch (1) controls the
inverted relief contrast, and the inner switch (2)
adjusts the positive relief contrast.
the opening of the built-in Fresnel lenses are
covered, which results in the different contrast
effects. Phase structures typically act as spatial,
relief-type images—in the positive relief
contrast like hills, in the inverted relief contrast
like valleys.
1
2
3
1
2
3
Switch for adjusting the inverted relief
contrast
Switch for adjusting the positive relief
contrast
Deflection mirror
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
98
Leica TL RCI™: Methods in Transmitted Light
Vertical bright-field illumination
Suitable for stained amplitude specimens with
sufficient contrast.
Inclined transmitted light
Single-sided dark field
Suitable for fixed specimens and fine struc-
tures.
Suitable for semitransparent, opaque speci-
mens such as foraminifera and fish eggs. Move
the deflection mirror until the desired data are
visible.
The light beams are deflected vertically through
the specimen. This results in an accurate bright
field with maximum brightness.
The flatter the angle at which the light beams
are deflected into the specimen plane, the
darker the substrate appears. A dark field-like
transmitted light is created. Outlines, fine edges
and structures are bright, in contrast with the
dark background, through diffraction of the
light beams on the dark background.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
99
Leica TL RCI™: Relief Images
Starting position
Positive relief contrast
Negative relief contrast
Suitable for semitransparent and transparent
specimens. The phase structures look like hills.
Suitable for semitransparent and transparent
specimens. These settings result in a nega-
tive relief contrast. Phase structures look like
valleys.
1. Push the deflection mirror all the way back
towards the column.
The effect can be strengthened or weakened by
gently tilting the deflection mirror.
2. Turn the deflection mirror into the notch
position at an angle of 45°.
The effect can be strengthened or weakened by
gently tilting the deflection mirror.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
100
Leica TL RCI™: Relief Images (cont'd.)
Dynamic relief contrast
Constraints
Suitable for semitransparent and transparent
specimens.
The relief methods provide good results from
mid-zoom to high magnifications and with 1×,
1.6× and 2× objectives. In the lower zoom half
and with weaker specimens, the object field
may not be uniformly illuminated.
By tilting the deflection mirror slightly, you can
move the gaps over the entire field of view and
quickly toggle between positive and negative
relief images. The dynamic effect makes it easy
to distinguish phase structures from amplitude
structures.
We recommend using the transmitted-light
base with 1× or higher objectives, and not
objectives with a long focal length.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
101
Using Filters
Filters for Leica TL RC™ and TL RCI™
The transmitted-light bases TL RC™ and TL RCI™
can be equipped with up to three filters—
available as accessories—simultaneously. By
customer request, the filters are also available
as one-off items.
2. Take the empty filter from an available filter
slot in the filter holder.
Daylight filter for Leica TL ST
A daylight filter is also available for the LeicaTL ST
transmitted-light base.
3. Insert the desired filter.
1. Switch off the light source or click
(Leica TL RCI™) the button for the shutter.
4. Switch the light source back on.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
102
Leica IsoPro™ (Non-motorized): Controls
Operating the Leica IsoPro™ mechanical
stage
1. To move the stage in the X direction, rotate
the outer knob.
2. To move the stage in the Y direction, rotate
the inner control ring.
1
2
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
103
Leica IsoPro™ (Motorized): Controls
IsoPro
X/Y Stage DCI module
Leica PSC Controller
1
1
2
2
3
1
2
3
4
1
2
3
4
Sub-D interface for Leica SmartMove™
3 CTL2 interfaces
USB interface (type B)
1
2
3
Quick control/memory function
Fine control in X direction
Fine control in Y direction
1
2
Leica IsoPro™ motorized mechanical stage
Housing with motorization
Socket for grounded power cable
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
104
Leica LED5000 MCI™
Use
Contact with the base
If the optics carriers are accidentally
lowered too far, contact between the
base and MCI may result. A safety mechanism
in the linkage ensures that, in this case, the MCI
is automatically pushed upwards to prevent the
possibility of damage.
The Leica LED5000 MCI™ (for "Multi Contrast
Illumination") is a universal high-output illu-
minator. Three groups of 3 LEDs each can be
arranged as desired around the specimen. The
targeted control of the LEDs enables various
illumination scenarios to be implemented.
•
Preparation
Installed too high
ρ
ρ
Be sure not to place any specimens directly
beneath the MCI.
Hold the MCI with both hands and pull it down-
wards until the illuminator clicks into place on
the bottom end of the guide rods.
After the optics carrier is lifted, put the MCI
back into the original position.
In this position, you always have the same
contrast with identical illumination. This guar-
antees the reproducibility of an experiment.
Optimum height
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
106
Leica LED5000 MCI™ (cont'd.)
Using the keyboard
The illumination scenarios
ρ
Use the key to switch the illumination on
or off.
Use the " " and " " keys to toggle between the
illumination scenarios shown below.
ρ
Use the "+" and "–" keys to adjust the
Maximum brightness
brightness in 10 increments. Touch either
of the two buttons to adjust the intensity in
small increments. Hold a key to change the
intensity more quickly.
For temperature reasons it is not possible to
switch on all nine LEDs simultaneously. There-
fore, to provide bright overall illumination, the
top two rows are activated. The bottom row
primarily provides contrast.
The Leica LED5000 MCI™ can also be controlled
by the LAS (Leica Application Suite) software.
Maximum brightness
Maximum contrast
Point illumination
from the rear
Point illumination
from the left
Point illumination
from the right
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
107
Leica LED5000 RL
Use
Using the keyboard
The illumination scenarios
Use the " " and " " keys to toggle between the
illumination scenarios shown below.
ρ
The Leica LED5000 RL ("Ring Light") generates
a very bright and homogenous incident light.
It has a diameter of 80 mm and illuminates the
specimen with 48 LEDs that can be switched on
and off completely or in various combinations.
It is controlled using either the integrated or via
the Leica Application Suite (LAS).
Use the key to switch the illumination on
or off.
ρ
Use the "+" and "–" keys to adjust the
brightness in 10 increments. Touch either
of the two buttons to adjust the intensity in
small increments. Hold a key to change the
intensity more quickly.
Constraint
The Leica LED5000 RL has been designed for
use with the 1.0× and 0.63× objectives. For the
1.6× and 2.0× objectives, conventional ring
illuminators from the Leica illuminator product
range are available.
LAS enables you to create fully reproducible
illumination scenarios and automatically toggle
between them. For additional information, refer
to the LAS online help.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
108
Leica EL6000 – About the Instrument
The Leica EL6000 compact light source is
intended for biological routine and research
work, including the examination of specimens
from the human body for the purpose of gain-
ing information.
Special features:
Heat-absorbing filter
The Leica EL6000 compact light source has an
automatic beam cover. This ensures that the
light beam output of the instrument when the
light guide is detached. This prevents the user
from being harmed by the intense light beam
generated by the instrument.
When the cover is open, the holder for the heat
filter can be accessed. Here, (hardened) heat-
absorbing filters for protecting the light guide
and various conversion filters with a diameter
of 32 mm for adapting the spectrum to the
application can be inserted.
The Leica EL6000 compact light
•
source is intended exclusively for
operating mercury short-arc lamps of type
HXP-R120W/45C VIS from OSRAM in conjunc-
tion with a suitable light guide for the instru-
ment. Any other use of the instrument shall be
considered noncompliant use.
The Leica EL6000 compact light source has a
built-in shutter. It can be operated at the instru-
ment itself by means of a pushbutton or via a
remote control input. The shutter is open when
the "open" LED is illuminated.
The Leica EL6000 comes factory-equipped with
a heat-absorbing filter.
The brightness can be varied in 5 increments
(0% – 100%). For this purpose, a mechanical
dimming unit that can be operated using a
rotary switch on the front of the unit is installed
in the device.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
109
Leica EL6000 – About the Instrument (cont'd.)
Compatible light guides
Identification of the instrument
The Leica EL6000 compact light source complies
with Council Directive 98/79/EC concerning in
vitro diagnostics. It also conforms to the Coun-
cil Directives 73/23/EEC concerning electrical
apparatus and 89/336/EEC concerning electro-
magnetic compatibility for use in an industrial
environment.
The Leica EL6000 compact light source is
compatible with liquid light guides that are
compatible with a "Storz long" light inlet and
whose transmission curve fits the lamp used.
Where applicable, refer to the lamp's data sheet
for its spectral light distribution.
The nameplate of the instrument is on its
bottom. It includes the necessary information
for identifying the instrument.
When connecting the light guide to the light
source or the microscope adapter, make sure
not to kink or damage the light guide.
Your Leica consultant will be glad to provide
additional information about suitable light
guides.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
110
Leica EL 6000 – Operation
Controls
Running-time meter
Shutter control
ρ
The running-time meter measures the burn-
ing time of the lamp, not the switch-on time
of the Leica EL6000 compact light source. The
running-time meter displays its current status
in this format: hours – minutes.
In the "open" position, the shutter of the
Leica EL 6000 is open—regardless of the
signal from the remote control.
ρ
If the shutter control is in the "remote" posi-
tion, the shutter is controlled by the remote
control.
1. Press the reset button to set the running-
time meter of the lamp to "0".
5
The running-time meter should be reset after
every lamp replacement so that it always
displays the current, accurate lamp life.
Intensity switch
The intensity switch provides optical dimming
of the light in five fixed increments.
4
3
2
1
Discontinue using the built-in lamp when it
reaches the end of the life specified by the
manufacturer.
1
2
3
4
5
Power switch/power indicator
Reset button for running-time meter
Running-time meter
Shutter control
Intensity switch
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
111
Leica EL 6000 – Troubleshooting
Fault description
Possible faults
Remedy
No power supply voltage present; connecting Check power supply voltage and connecting
cable not plugged in correctly/defective
Fuses in instrument defective
cable
Power indicator is not illuminated after being
switched on, instrument shows no function
Replace fuses, use permitted replacement
type only (see Page 113)
Housing cover loose
or removed
Attach housing cover securely
Power switch is illuminated after being switched
on, instrument shows no function
Lamp worn out
Replace lamp
Shutter closed or intensity switch at left limit
stop
Open shutter or rotate intensity switch
towards the right
Target system receives too little light or none at all Light guide not inserted into light guide
output properly
Check light guide
Light guide damaged/defective
Repair or replace
If these actions do not fix the problem, send the Leica EL6000 compact
light source to Leica service with an exact description of the malfunction.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
112
Cleaning and Maintenance
Before carrying out cleaning tasks,
switch off the Leica EL6000 compact
light source.
Spare parts and accessories
Permitted replacement fuses
•
The only parts of the Leica EL6000 compact
light source that may be replaced by the user
are the instrument fuses and the lamp.
Fuse: 5×20, 2.5 A, slow-blowing, breaking capacity H
Wickmann Series 181
www.wickmann.com
Schurter Series SPT 5×20 www.schurter.ch
Use only a dry, lint-free cloth for cleaning. When
cleaning, ensure that no dust or other foreign
bodies enter the device through the ventilation
slots.
▶
The replacement of the lamp is described
on Page 61.
Repairs
Opening or working on the Leica EL6000
compact light source in any manner other than
described above (changing lamps, changing
fuses) may be carried out by the supplier of the
instrument or the supplier's authorized repre-
sentatives.
Replacing the instrument fuse
1. Disconnect the Leica EL6000 from the
power supply.
In case of damage (even external damage),
immediately stop using the Leica EL6000
compact light source and send it to service.
2. Open the drawer with the instrument fuses
located close to the power supply input.
If it will not be used for a long time, protect the
Leica EL6000 compact light source from dust
using a suitable cover (such as the plastic bag
from the original packaging).
3. Replace the instrument fuse.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
113
Filter Changer
The patented FLUOIII® filter system consists of
a filter changer for barrier and excitation filters,
a UV shutter that can be activated/deactivated,
and a filter insert for a filter holder with your
choice of filter.
If you are using fewer than four filter
holders with fluorescence filters, always
The simple filter holders also protect the
system from dust.
•
insert the included simple filter holders with
closed illumination beam path into the avail-
able positions. Otherwise, there is a risk of
danger to the eyes from direct UV radiation
from the third beam path.
Filter sets for fluorescence
The filter sets for fluorescence contain, on one
filter holder, two barrier filters to the visual
beam paths and one excitation filter to the illu-
mination beam path. The fluorescence filters
have a transponder with the specific filter data
for use with the motorized Leica M205 FA. The
filter sets are labeled (see Page 118).
The filter changer can hold a total of 4 filter sets.
The filter sets for fluorescence are labeled (see
Page 118). All filter sets are equipped with a tran-
sponder for automatic filter detection and can
also be used with the motorized Leica M205 FA.
The scope of delivery of the Leica M205 FA and
Leica M165 FC includes three simple filter hold-
ers without fluorescence for empty filter posi-
tions and transmitted or incident light observa-
tion without fluorescence. These filter holders
have two empty openings for the observation
beam paths. The third opening, to the illumina-
tion beam path, is closed.
A selection of the available filters
including simple filter holder (bottom right)
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
115
FIM – Fluorescence Intensity Manager
FIM consists of a diaphragm disk with pinhole
diaphragms of varying transmission capaci-
ties. The intensity of the excitation light can
be reduced in five fixed steps. The light can
be changed from 100% to 55%, 30% and 17%
down to 10%.
The advantage of this procedure is its absolute
reproducibility and fast switching between the
intensity levels. A separate FIM level can be
stored for every fluorescence filter. Different
intensities of fluorescence can thus be cali-
brated to one another.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
116
About Fluorescence Microscopy
Functional principle
The Leica M Series
The filter system
Fluorescent substances light up when irradi-
ated with short-wave excitation light, and fluo-
rescence microscopy takes advantage of this
property. Specimens without self-fluorescence
are stained with a fluorescent substance. One
such dye is green fluorescent protein (GFP),
which is used in molecular biology.
The Leica M series fluorescence stereomicro-
scopes allow entire fluorescent specimens to
be studied, unprepared and in three dimen-
sions. The fully apochromatic optics system and
the enormous zoom range make the Leica M
series stereomicroscopes your first choice. The
patented TripleBeam® third beam path for fluo-
rescence illumination and the patented FLUOIII®
filter system generate highest-quality fluores-
cence images. The very bright high-pressure
mercury burner and specially selected filter sets
enable you to distinguish the finest structures
and enhance the quantity of information for
incident light fluorescence.
The FLUOIII® filter system consists of a rapid
filter changer for barrier and excitation filters
and a filter insert for a filter holder with your
choice of filter. A total of four filter sets (which
are labeled) can be used in the rapid filter
changer. An empty filter cartridge is also avail-
able for individual filter combinations. Each
filter set includes two barrier filters to the visual
beam paths and an excitation filter to the illu-
mination beam path.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
117
Rapid Filter Changers and Filter Types
The rapid filter changer must be equipped with four filter holders at all times.
Filter sets
GFP
Excitation filter
Barrier filter
Designation
425/60 nm (395–455 nm)
480/40 nm (460–500 nm)
470/40 nm (450–490 nm)
360/40 nm (340–380 nm)
425/40 nm (405–445 nm)
470/40 nm (450–490 nm)
546/10 nm (541–551 nm)
436/20 nm (426–446 nm)
510/20 nm (500–520 nm)
560/40 nm (540–580 nm)
545/30 nm (510–560 nm)
620/60 nm (590–650 nm)
555/60 nm (530–580 nm)
480 LP
GFP1
GFP2
GFP3
UV
GFP Plus
GFP plants
UV
510 LP
525/50 nm (500–550 nm)
420 LP
Violet
Blue
460 LP
V
515 LP
B
Green
CFP
590 LP
G
480/40 nm (460–500 nm)
560/40 nm (540–580 nm)
610 LP
CFP
YFP
TXR
DSR
CY5
CY3
YFP
Texas Red
DsRED
CY5
620/60 nm (590–650 nm)
700/75 nm (663–737 nm)
610/75 nm (573–648 nm)
CY3
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
118
Simple filter holders
Simple filter holders
Safety Notes
If you are using fewer than four filter
holders with fluorescence filters, always
ρ
Three simple filter holders are included with
the Leica M165 FC and the Leica M205FA. These
filter holders have two empty openings for the
observation beam paths. The third opening, to
the illumination beam path, is closed.
If you want to block the mercury light for
•
longer than that, activate the UV shutter.
insert the included simple filter holders with
closed illumination beam path into the avail-
able positions. Otherwise, there is a risk of
danger to the eyes from direct UV radiation
from the third beam path.
ρ
ρ
Use the filter if you want to change to trans-
mitted light or incident light observation
without fluorescence.
ρ
Use this filter holder if you want to work
briefly (no more than 15 seconds) without
fluorescence illumination.
Always place a simple filter holder into the
empty filter changer positions in order to
protect yourself from UV radiation and
protect the system from dust.
So that the filter holder is not heated,
it must not block the mercury light for
longer than 15 seconds.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
119
Equipping the Filter Changer
When inserting the filter sets, avoid
Removing the simple filter holder
Inserting the filter
•
touching the filters in order to avoid
fingerprints. Clean dirty filters immediately
using a soft, lint-free cloth and pure alcohol.
The Leica M165 FC and the Leica M205FA are
equipped at the factory with simple filter hold-
ers as "dummies". These must be removed
before inserting a filter.
1. Place the filter into the filter changer.
Ensure that the contour of the filter holder
matches the contour of the microscope.
1. Grasp the recess of the dummy in the filter
changer and pull it out.
2. Rotate the filter changer by hand until it
latches into place.
If the filter holder has not been inserted
correctly, the filter changer does not rotate.
3. Insert a total of four filters.
Slot for filter slide
There is a slot in the filter changer system for
an individually selectable filter, for example a
neutral density filter.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
120
Observation without Fluorescence
We recommend that you first familiarize
yourself with the controls of your Leica
fluorescence microscope in transmitted light
without the presence of fluorescence illumina-
tion.
Observation without Fluorescence
Close the UV shutter when you do not want to
view the specimen with fluorescence illumina-
tion, but only with transmitted light.
The FLUOIII® filter system includes a UV shutter
that can be used to close the illumination beam
path. This feature protects the mercury lamp,
which should not be switched on and off too
frequently. If, for instance, you need to inter-
rupt your work, you do not have to switch off
the high-pressure mercury burner to preserve
sensitive specimens from being burned out or
bleached out.
After switching on the high-pressure
•
mercury burner, you must adjust the arc
lamp immediately (see User Manual M2-216-1).
We recommend that you first familiarize
yourself with the basic functions of your
Leica stereomicroscope as they are described in
the previous section of this User Manual. These
include the binocular tube, the eyepieces, the
zoom magnification changer, the focusing
drive, and others.
Switching the high-pressure mercury
burner on and off frequently decreases
its life and causes delays, as the lamp can only
be switched back on after it has cooled. Instead,
you can close the UV shutter and reopen it
when you return to your workplace.
The following describes the specific functions
of the fluorescence system only.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
121
Double Iris Aperture
The built-in double-iris diaphragm regulates
the depth of field. Note that:
ρ
The greater the depth of field, the darker
the image becomes.
ρ
In the same way, the exposure time
becomes longer for photographs.
ρ
The resolution decreases.
Adjusting the double-iris diaphragm using the
knurled ring
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
122
Commissioning the Fluorescence System
After switching on the high-pressure mercury
burner, you must adjust the arc lamp imme-
diately (see User Manual M2-216-1). Do not
switch on the supply unit until you feel confi-
dent using the Leica stereomicroscope.
For observation with fluorescence light,
always position the protection screen
correctly to protect the observer (see Page 27).
•
Follow all safety instructions when
•
handling mercury lamps and the lamp
housing!
1. Switch on the supply unit (see User Manual
M2-216-1).
2. Wait 2–3 minutes.
3. Adjust the arc lamp in accordance with User
Manual M2-216-1.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
123
Leica SmartMove
With its non-slip controllers and knobs, the
Leica SmartMove control unit offers the right
feel for handling motorized Leica instruments.
Controls
The functions of the individual controls can be
configured both directly on the Leica Smart-
Move and using the Leica Application Suite.
For a detailed description of the functions and
settings, please refer to the Leica SmartMove
manual or the Leica Application Suite help file.
Download from Www.Somanuals.com. All Manuals Search And Download.
Leica M-series
User Manual
125
Leica SmartTouch™
ρ
ρ
ρ
ith the touchscreen of the Leica SmartTouch™,
an control your experiment procedures
a few hand movements and never lose
of all your important optical parameters.
Top part of the knob = zoom
Upper part of the knob = focus
Buttons = left filter, right filter, shutter
most important control functions on the
ol unit can be adjusted to your specific
with freely programmable rotary knobs
nction buttons.
For a detailed description of the functions and
settings, please refer to the Leica SmartTouch™
manual or the Leica Application Suite help file.
ols
unctions of the individual knobs and
n elements can be configured both directly
e Leica SmartTouch™ and using the Leica
ation Suite.
andard configuration when shipped from
the factory is as follows:
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
126
Leica PSC Controller
Use
Passing over the specimen quickly
Control by LAS
ρ
The Leica PSC controller gives you precision
control of the Leica IsoPro™ motorized mechan-
ical stage.
Move the joystick in any desired direc-
The instructions for controlling the Leica IsoPro™
using the Leica Application Suite are provided
in the software's help file.
tion (including diagonally) to control the
mechanical stage quickly.
Connection
Connect the Leica PSC controller and the Leica
IsoPro™ motorized mechanical stage to the
DCI box.
Fine control of the mechanical stage
The motorized Leica IsoPro™ mechanical stage
offers an accuracy of up to 0.25 µm. To move to
a position with maximum accuracy, use the left
and right knobs on the joystick.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
127
Leica M165 FC (cont'd.)
Leica M165 C with TL RCI™ transmitted light stand, Leica IsoPro™ manual mechanical stage and manual focusing drive
292
217
154
187
497
X = +-75 mm
370
max. 370
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
130
Leica M165 FC (cont'd.)
Leica M165 C with TL RCI™ transmitted light stand and manual focus
292
217
154
154
340
356
Download from Www.Somanuals.com. All Manuals Search And Download.
Leica M-series
User Manual
131
Leica M205 FA (cont'd.)
Leica M205 FA with TL RCI™ transmitted light stand, ErgoTrinoc tube, DFC camera and motorized focusing drive
292
340
154
154
385
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
133
Leica M205 FA (cont'd.)
Dimensions of Leica M205 FA with TL RCI™ transmitted light stand, IsoPro™ mechanical stage, ErgoTrinoc tube, DFC camera and motorized
focusing drive
292
154
187
497
X = +-75 mm
370
max. 410
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
134
Leica M165 FC / Leica M205 FA
Leica M165 FC
Leica M205 FA
Optical data
Zoom
16.5 : 1 manual
20.5 : 1 automated with FusionOptics™
Data with standard optics (1×objec-
tive/10× eyepieces)
– Zoom range
7.3×–120×
max. 453 lp/mm
61.5 mm (planapochromat)
∅ 31.5 mm – 1.92 mm
7.8×–160×
max. 525 lp/mm
61.5 mm (planapochromat)
∅ 29.5 mm – 1.44 mm
– Resolution
– Working distance
– Object field
Maximum values (based on optics combi-
nation)
– Magnification
960×
1280×
906 lp / mm
551 nm
0.302
1050 lp / mm
476 nm
0.35
– Resolution
– Visible structural width
– Numerical aperture
– Object field
∅ 63 mm
∅ 59 mm
Working distances
135 mm (0.5× planachromat)
112 mm (0.8× planachromat)
67 mm (0.63× planapochromat)
61.5 mm (1× planapochromat)
30.5 mm (1.6× planapochromat)
20.1 mm (2× planapochromat)
135 mm (0.5× planachromat)
112 mm (0.8× planachromat)
67 mm (0.63× planapochromat)
61.5 mm (1× planapochromat)
30.5 mm (1.6× planapochromat)
20.1 mm (2× planapochromat)
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
139
Leica M165 FC / Leica M205 FA (cont’d.)
Leica M165 FC
Leica M205 FA
Optics carrier
100% apochromatic optical system
CMO (Common Main Objective) lead-free
CMO (Common Main Objective) lead-free
Specific surface resistivity
(housing)
2×1011 Ω/mm2
discharge time <2 seconds from 1000V to 100V
2×1011 Ω/mm2
discharge time <2 seconds from 1000V to 100V
Encoded/automated (M205 FA)
Zoom, iris diaphragm, objective nosepiece
Zoom, iris diaphragm, objective nosepiece,
FIM, filter
Engageable zoom notches
13 for repetitive tasks
Built-in and encoded
14 for repetitive tasks
Built-in and motorized
Double-iris diaphragm for depth of field
control
FIM ( Fluorescence Intensity Control )
–
Built-in and motorized
1× planapochromat
Accessories
Standard objective (parfocal in the objec-
tive nosepiece)
1× planapochromat
Additional objectives (parfocal in the
objective nosepiece)
5× planachromat, 2× planapochromat, 1.6×
planapochromat, 0.63× planapochromat
5× planachromat, 2× planapochromat, 1.6×
planapochromat, 0.63× planapochromat
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
140
Leica M165 FC / Leica M205 FA (cont’d.)
Leica M165 FC
Leica M205 FA
Additional objectives (non-parfocal)
Interpupillary distance
1× planachromat, 0.8× planapochromat, 0.63×
planapochromat, 0.5× planapochromat
1× planachromat, 0.8× planapochromat, 0.63×
planapochromat, 0.5× planapochromat
50 mm–105 mm
50 mm–105 mm
Wide-field eyepieces for persons wearing
glasses
10×, 16×, 25×, 40×, with replaceable
eyecups & click stop
10×, 16×, 25×, 40×, with replaceable
eyecups & click stop
Manual coarse/fine focus
Motorized focusing drive
Focusing range 130 mm,
adjustable ease of movement
Focusing range 130 mm,
adjustable ease of movement
Motorized focusing drive with 431 mm profile
column, with power supply
Motorized focusing drive with 431 mm profile
column, with power supply
Computer interface
USB
USB
Module System
Stands, illuminators
Transmitted light stands
For bright field and bright/dark field, high-performance base: Bright field, single-sided dark field,
with Rottermann Contrast and CCIC
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
141
Leica M165 FC / Leica M205 FA (cont’d.)
Leica M165 FC
Leica M205 FA
Incident light stand
Stages
Large incident light base with black/white stage plate and Antishock™ feet
Gliding stage, MATS heating stage, cup stage, rotatable polarization stage,
Leica IsoPro™ mechanical stage (manual and motorized)
Illuminators
LED5000 MCI™ and LED5000 RL, fluorescence, oblique, coaxial, cold light sources
Misc. accessories
Photography, video
– Leica DFC digital image capture system, various camera variants, Leica IC D
– Leica IC A video module
– Various adapters for commercially available CCD cameras
Image archiving, image processing
Leica AF6000: Special software for all fluorescence tasks
Leica Application Suite (LAS): consisting of basic program and various auxiliary modules
Measurement graticules
Vertical and oblique observation
Drawing tube
For length measurements and counting
45° side view around the complete object
For both left and right-handed users
For detailed information about optical accessories, binocular tubes, stands, illuminators
and additional accessory modules for various applications, refer to the brochure
"Leica M Stereomicroscopes – The Modular System", M1-105-4en.
Download from Www.Somanuals.com. All Manuals Search And Download.
Leica M-series
User Manual
142
Leica EL6000 – Specifications
Dimensions (W×H×D)
Width×height×depth
Electrical safety
in accordance with EN 61010-1:2001
Pollution degree
130 mm × 210 mm × 290 mm
Environmental conditions
Permitted ambient temperature
Permitted relative humidity
2
II
-20 to 85 °C
Overvoltage category
Power input
Design
10% to 90%,
non-condensing
Switched socket IEC320 C13
(EN 60320 C13)
Operation
Permitted ambient temperature
Permitted relative humidity
Operational data
Application area
0 to 40 °C
Power supply voltage
Power frequency
100 to 240 VAC ( 10%)
50 to 60 Hz
10% to 90%, non-condensing
Fuse protection
2x T2.5 A; Breaking capacity
H (manufacturer: Wickmann;
Series 181)
Enclosed rooms
Altitude for use
Max. 2000 m above sea level
Protective class
I
Power consumption
Max. 210 VA
Type of protection
EMC
Emitted interference
Immunity to interference
Voltage fluctuations, flicker
Harmonic currents
IP20 (EN 60529)
Lamp
Type
Mercury short-arc reflector
lamp
EN 61326 (Class B)
EN 61326 Appendix A
EN 61000-3-3
Designation
OSRAM HXP-R120W/45C VIS
120 W
Power consumption
EN 61000-3-2
Service life (manufacturer information) Average value 2000 h
The instrument fulfills the requirements of EC Directive 89/336/EEC and
the EMC law of September 18, 1998
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
143
Leica EL6000 – Specifications (cont'd.)
Light output
Protective functions
Design
Type: Storz, long
Safety circuit
If the cover is open, all components in the instru-
ment (except for the power input and power plug)
are disconnected from the power supply voltage.
In the event of overheating, the lamp supply is shut
off. After cooling off, the lamp power supply in the
Leica EL6000 compact light source is automatically
switched on again.
Special feature
Self-locking if no light guide is
plugged in
Overheating
protection
Fan
Switch-on delay
Approx. 60 s after lighting the
lamp
Interface
Protection screen If no light guide is plugged into the light output of
the instrument, the light output is automatically
closed off so that no light can escape.
Type
Remote control input for
internal shutter
Design
9-pin D-Sub socket
Remote signal level
– Shutter closed
– Shutter open
– Input current
Footswitch
–8 to +0.8 V
+2.4 to +8 V
Approx. 1 mA
– Shutter closed
Connection between
pin 2 and 4
– Current
Approx. 1.2 mA
Max. 10 Hz
Shutter frequency
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
144
Leica TL ST Transmitted-light Base
Light source
Halogen lamp 12V/20W
Quick illuminant change
Illuminated area
Power supply unit
Yes
50 mm
Input voltage 100–240 V~, frequency 50–60 Hz
energy consumption 30 W max.,
ambient temperature 10 – 40 °C
Power plug, power switch
7.4 kg
Connections
Weight
Illumination types
Bright field
Yes
Dark field
Yes (single-sided)
Oblique light
No
No
Relief Contrast System (RC™)
CCIC (Constant Color Intensity Control) No
Internal shutter/lamp control
Integrated filter holder
Coated optics for increasing the color
temperature
No
Yes
Yes
Matching of high num. aperture
Remote control options
AntiShock™ Pads
No
No
Yes
Dimensions (W×H×D)
340×430×85 mm
Download from Www.Somanuals.com. All Manuals Search And Download.
Leica M-series
User Manual
145
Leica TL BFDF Transmitted-light Base
Light source
External via cold light source
Illuminated area
Connections
40 mm
Connection for cold light guide,
active f=10mm, end tube f=13mm
5.8 kg
Weight
Illumination types
Bright field
Yes
Yes
No
No
Dark field
Oblique light
Relief Contrast System (RC™)
CCIC (Constant Color Intensity Control) No
Internal shutter/lamp control
Integrated filter holder
Coated optics for increasing the color
temperature
Yes*
No
No
Matching of high num. aperture
Remote control options
AntiShock™ Pads
Yes**
Yes***
Yes
Dimensions (W×H×D)
340×390×90 mm
*With cold light source Leica CLS150 LS **Concave mirror *** With external light source
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
146
Leica TL RC™ / TL RCI™
Leica TL RC™
Leica TL RCI™
Light source
External via cold light source
Halogen lamp 12V/20W
Yes
Quick illuminant change
Illuminated area
Power supply unit
–
35 mm
–
35 mm
100–240 V~, frequency 50–60 Hz
energy consumption 30 W max.,
ambient temperature 10 – 40 °C
1×USB type A, 1×USB type B, 2×CAN-BUS
Connections
Weight
Connection for cold light guide,
active f=10mm, end tube f=13mm
6.0 kg
7.2 kg
Illumination types
Bright field/dark field
Yes/yes
Yes/yes
No
Yes/yes
Oblique light/Relief Contrast System (RC™)
CCIC (Constant Color Intensity Control)
Internal shutter/lamp control
Integrated filter holder
Yes/yes
Yes
Yes**
Yes
Yes
Yes
Coated optics for increasing the color temperature
Matching of high num. aperture
Remote control options
Yes
Yes
Yes***
Yes****
Yes
Yes***
Yes
AntiShock™ Pads
Yes
Dimensions of base (W×H×D)
340×390×95 mm
340×440×95 mm
*Single-sided **With cold light source Leica CLS150 LS ***Concave mirror ****With external light source
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
147
Leica IsoPro™ Motorized XY Stage
Compatibility
Stage size (L ×W × H)
Travel
Leica incident light base and TL bases (TL BFDF, TL RC™, TL RCI™)
335.5 mm × 370 mm × 41.5 mm
152 mm × 102 mm (6" × 4")
Speed X,Y
30 mm/s
Motor resolution X,Y
Repeatability X,Y
Positioning accuracy X,Y
Hysteresis X, Y
Motor
0.25 µm
2 µm
20 µm over the entire positioning range
30 µm
Direct current (DC) motor with encoder
Backlash
Slack-free
Adapter
All adapters for TL bases
Acceleration
S-shaped acceleration
Computer connection
Control options
Power supply
USB
Leica PSC™, Leica Smart Move, Leica SmartTouch™, Leica LAS via PC
Input: 100–240VAC 50 / 60 Hz 1.0 A
Output: 15 VDC 2.66 A 40W max.
Download from Www.Somanuals.com. All Manuals Search And Download.
Leica M-series
User Manual
148
Calculating the Total Magnification and Field of View Diameter
Parameter
Example
MO
ME
z
Magnification of the objective
MO
ME
z
1× objective
Magnification of the eyepiece
25×/9.5 eyepiece
Magnification of the changer position
Zoom position 4
q
Tube factor, e.g. 1.5× for coaxial incident light, 1.6× for 45°
ErgoTube™
q
Coaxial reflected light 1.5×, tube factor
Factor 1.25×
r
r
Factor 1.25x if the planachromatic and planapochromatic
objectives of the MZ125/MZ16 are used on the MS5, MZ6,
MZ75 or MZ95
NFOV Field number of the eyepiece. Field numbers are printed on the
eyepieces: 10× = 21, 16× = 14, 25× = 9.5, 40× = 6.
Calculation example: Magnification in the binocular tube:
Calculation example: Field of view diameter in the specimen
MTOT VIS = MO × ME × z × q × r
or
1 × 25 × 4 × 1.5 × 1.25 = 187.5×
N
FOV
∅ OF:
M
O × z ×q ×r
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
150
Care, Maintenance, Contact Persons
We hope you enjoy using your high-perfor-
mance microscope. Leica microscopes are
renowned for their robustness and long service
life. Observing the following care and clean-
ing tips will ensure that even after years and
decades, your Leica microscope will continue to
work as well as it did on the very first day.
Contact address
Care
ρ
However, if your instrument should no longer
function properly, contact your technician,
your Leica representative or Leica Microsystems
(Switzerland) AG, CH-9435 Heerbrugg.
Protect your microscope from moisture,
fumes and acids and from alkaline, caustic
and corrosive materials and keep chemicals
away from the instruments.
ρ
Plugs, optical systems and mechanical parts
must not be disassembled or replaced,
unless doing so is specifically permitted
and described in this manual.
E-mail contact:
Warranty benefits
The warranty covers all faults in materials
and manufacture. It does not, however, cover
damage resulting from careless or improper
handling.
ρ
ρ
Protect your microscope from oil and
grease.
Do not grease guide surfaces or mechani-
cal parts.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
151
Care, Maintenance, Contact Persons (cont'd.)
Protection from dirt
Dust and dirt will affect the quality of your
results.
Cleaning polymer components
Permitted measures
ρ
Some components are made of polymer or are
polymer-coated. They are, therefore, pleasant
and convenient to handle. The use of unsuitable
cleaning agents and techniques can damage
polymers.
Clean the microscope (or parts of it) using
warm soapy water, then wipe using distilled
water.
ρ
Put a dust cover over the microscope when
ρ
it will not be used for a long time.
For stubborn dirt, you can also use ethanol
(industrial alcohol) or isopropanol. When
doing so, follow the corresponding safety
instructions.
ρ
Use dust caps to protect tube openings,
tubes without eyepieces, and eyepieces.
ρ
ρ
Keep accessories in a dust-free place when
Remove dust with a pneumatic rubber bulb
not in use.
or with a soft brush.
ρ
Clean objectives and eyepieces with
special optic cleaning cloths and with pure
alcohol.
Download from Www.Somanuals.com. All Manuals Search And Download.
User Manual
Leica M-series
152
|