Leica Stereo-
Fluorescence
Systems
User Manual
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Contents
Page
Leica MZ10 F
fluorescence stereomicroscope
Description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4
Fluorescence applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6
Components, controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8
Sets of filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9
Assembly
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10
Focusing drive, microscope carrier, MZ10 F optics carrier . . . . . . . . . . . . . . . . . . . 11
UV protection screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11
Model 106Z lamp housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Mercury-vapour lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Stray-light protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Collector, heat-protection filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Supply units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Adjusting the mercury-vapour lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Operation
FLUOIII™ filter system, light stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16
Adjusting the dioptric setting, focusing, observing . . . . . . . . . . . . . . . . . . . . . . . . . . 17
Leica stereo-fluorescence module
Components, controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
Sets of filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19
Assembly
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20
Focusing drive, microscope carrier, optics carrier . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Fluorescence module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
Sets of filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21
UV protection screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22
Model 106Z lamp housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Mercury-vapour lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12
Stray-light protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Collector, heat-protection filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13
Supply units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14
Adjusting the mercury-vapour lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15
Operation
Filter changer, light stop, double-iris diaphragm . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23
Adjusting the dioptric setting, focusing, observing . . . . . . . . . . . . . . . . . . . . . . . . . . 23
3
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Description
Safety concept
Before you set up the instrument and before you fit the
fluorescence illuminator, read:
Fluorescence technique
•
this user manual, observing the notes relating to safety
Some substances fluoresce when irradiated with short-wave
light. This property is utilized in the fluorescence technique,
in which certain structures and features which do not fluo-
rescence can be tagged with a fluorescing dye. An example
is green-fluorescing protein (GFP), which is used in molecular
biology.
•
the user manual for your stereomicroscope, observing the
notes relating to safety and care.
Permitted uses
Leica stereo-fluorescence systems are equipped with a special
fluorescence illuminator. They are intended for the three-
dimensional observation of fluorescing objects. They consist of:
Stereo-fluorescence
With Leica fluorescence systems you can complete unprepared
fluorescing specimens to be non-destructively studied in three
dimensions, manipulated, sorted and recorded. The intense light
emitted by the mercury-vapour burner, used in conjunction with
selected filter sets, enables even the finest structures to be
differentiated and expands the amount of information revealed
by the incident-light fluorescence technique.
–
–
–
a stereomicroscope with stand, binocular tube and
eyepieces
an integrated FLUOIII filter system (for MZ10 F)
or a separate stereo-fluorescence module
appropriate filter sets with barrier- and excitation filters,
a light stop, and individually-selectable filters
–
–
–
a UV protection screen
Fluorescence stereomicroscope
a lamp housing with high-pressure mercury vapour burner
a supply unit with power cable.
The Leica MZ10 F with 10:1 zoom is the routine stereo-
microscope for fluorescence applications. The patented sepa-
rate beam path (TriBeam™) for the fluorescence illuminator and
the patented filter system (FLUOIII™) together produce excellent
fluorescence images.
Prohibited uses
The use of Leica stereo-fluorescence systems in a different
manner from that described in this user manual can lead to inju-
ry, malfunction and damage.
Stereo-fluorescence module
The stereo-fluorescence modul can be combined with the
Leica stereomicroscope models MS5, MZ6, MZ7.5, MZ9.5,
MZ12.5, MZ16, and with older models.
–
Do not fit different plugs or cables
–
Do not dismantle or modify components unless instructions
for doing so are given in the user manual
–
Components may only be opened by authorized personnel.
User manual
The present user manual describes only the functions of the
fluorescence illuminator, the use of the filter sets, and the fitting
of the fluorescence module. For detailed information about the
use of the stereomicroscope, its care and its safety, please refer
to those sections of the separate manual M2-105-0EN which
relate to the Leica MZ12.5 with 12.5:1 zoom.
Place of use
Leica stereo-fluorescence systems are intended for use in
closed rooms and may not be used outdoors.
Responsibilities of person in charge of instrument
–
Ensure that the Leica stereo-fluorescence systems are
operated, maintained and repaired only by authorized and
trained personnel
–
Ensure that personnel who use the instrument have read
and understood this user manual and in particular all safety
instructions.
4
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Supply unit
Servicing and repairs
•
Pull the power plug of the supply unit out from the power sok-
ket:
–
Repairs may only be carried out by Leica-trained service
technicians or by technical personnel authorized to do so by
the person in charge of the instrument
–
–
–
when assembling and dismantling the lamp housing
before opening the lamp housing
–
–
Only original Leica spare parts may be used
Unplug the power cable before opening the supply unit.
Touching live circuits can cause injury.
when changing the mercury-vapour burner and other
components such as the heat-protection filter or the collector
–
when servicing the supply unit
Legal requirements
Adhere to general and local regulations relating to accident
prevention and environmental protection.
Lamp housing
•
•
•
Never open the lamp housing while the burner is switched on
(risk of explosion, UV irradiation, dazzling)
Conformity with European Community directive
Leica stereo-fluorescence systems and their accessories are
constructed in accordance with the latest technologies and are
provided with a statement of conformity with EC requirements.
Always allow the lamp housing to cool for at least 15 minutes
before opening it (risk of burner exploding)
Never cover the ventilator slots on the lamp housing (risk of fire)
Mercury-vapour lamp
•
Read the user manual and safety directions provided by the
manufacturer of the mercury-vapour burner, and particularly
those relating to its breakage and to the release of mercury
•
Before transporting the equipment, remove the mercury-vapour
burner and place it in its original packaging. Use the transport
peg in place of the burner, to secure movable parts within the
lamp housing
Light source: Safety regulations
Safety measures introduced by manufacturer
•
•
When the mercury-vapour burner has reached the end of its
nominal working life as indicated by the manufacturer’s
information and the time counter on the supply unit, change the
discoloured burner (increased risk of explosion)
–
UV protection screen in front of the specimen plane prevents
direct UV radiation from reaching the eyes.
–
Dummy filter carriers in the unoccupied positions of the rapid
filter changer prevent direct UV radiation from reaching
the eyes
Leica declines all responsibility for injury and damage
resulting from exploding, incorrectly-fitted or incorrectly-used
mercury-vapour burners.
–
–
UV filters in the observation beam paths protect the eyes
against UV radiation.
Stray-light protection on the underside of the lamp housing
protects the user's hands against UV radiation.
Warning
UV can damage your eyes. Therefore:
–
–
–
–
Never look at the light spot on the specimen plane without
the UV protection screen in position
Never look into the eyepieces unless there is an excitation
filter in the beam path
Always keep dummy filter carriers in the unoccupied
positions of the rapid filter changer (for MZ10 F)
Do not place the specimen on a white or highly-reflective
background.
5
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Applications
Natural science
Application
Fluorescence filter set
Anatomy
Biology
• Monitoring of capillary flow
• GFP 1, GFP 2
• GFP 1, GFP 2
• Gene expression in chicken embryos, fruit flies,
threadworms and zebra fish
• Fish otoliths marked with alizarine red
• Genetic expression through viruses and bacteria
which infect plants and animals
• Green
• GFP 2
Biomedicine
Genetics
• Humatic seals on pacemakers
• Blue, green
• GFP 1, GFP 2
• Cellular detection and protein expression
• Sorting and dissection
• Monitoring developmental processes
Neurology
• Gap junctions on muscles and nerves
• Cell development in rats' eyes
• GFP 1, violet
• GFP 1, GFP 2
Ophthalmology
Pharmacy
• Drugs
• GFP 1, GFP 2
• Green
• GFP 2
• ELI spotting in cell structures
• Monitoring of capillary flow with FITC
Plants and seeds
• Plants, genetic expression, transgenetics
• Bacteria detection
• GFP 1, GFP 2
• GFP 3
Botany
• Plant cells, plant surfaces, soil samples
Hydrology
• Water quality (bacterial and other pollutants)
• Filtered water
• Cell structures in and on the filter membrane
• UV
• GFP 1
• GFP 1
Parasitology
Forestry
• Detection of bacteria on ticks
• GFP 1, GFP 2
• Development of environmentally-acceptable methods of pest control • GFP 1
(investigation of viruses on pests)
6
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Industry
Application
Fluorescence filter set
Electronics
• Solder paste on SMDs
• Epoxy resin on SMD plates
• Luminescent coating of TV monitor tubes
• Quality of polymer castings for embedding integrated circuits
• GFP 1, GFP 2
• GFP 1, GFP 2
• GFP 1, GFP 2
• Violet
Semiconductors
• Positive photo resists (OCG HPR 504)
• Negative photo resists
• GFP 1, green
• Blue
• Detection of foreign particles on masks, wafers and
• subassemblies
• UV, GFP 1, GFP 2
• Violet
Petrochemicals
Polymers
• Oil inspection
• GFP 1
• Detection of foreign particles
• Blue
• UV
• GFP 1, GFP 2
• Identification of non-polymerized parts
• Examination of beads (polymer pellets used in chemical
measurements and analyses)
Engineering
• Inspection of cemented areas on mechanical
or optical components
• GFP 1, violet
• GFP 1
Metalworking
industries
• Cracks and surface defects
• Detection of contamination on components
• Quality control of welds
• Fracture analysis
Materials science
• Cracks, fractures, welds
• Carbon bonding materials; fractures and orientation
of carbon fibres
• GFP 1
• Violet
Bitumen
• Quality control for tar and bitumen
• Cracks and pores
• GFP 1
• GFP 1
Concrete
Papermaking
• Coating of paper fibres
• Investigation of inclusions
• GFP 1
• Violet
Forensic work
• Textile fibres, fingerprints, banknotes,
forgeries, body fluids
• GFP 1, GFP 2
Art restoration
Gemmology
• Pigments, forgeries
• UV
Quality, value, inclusions
• Blue
7
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Overview
3e
4a
3c
Leica MZ10 F: Its components and controls
1. Choice of stand and of focusing drive
8
(manual or motor-driven)
2. Microscope carrier for Leica MZ10 F
3a. Leica MZ10 F optics carrier with third beam path
3b. Integrated FLUOIII filter system
3b
3d
3c. Adapter for light source
3d. Rapid filter changer for any of four sets of filters
4b
3a
2
3e. Slots for light stop and for filter slide with
individually-selectable filter
4a. Leica 106Z lamp housing for 50W or 100W
high-pressure mercury vapour burners
6
4b. Stray-light protection
5. Supply units for 106Z lamp housing (not illustrated)
6. UV protection screen with arm
7. Double-iris diaphragm (optional)
1
8. Choice of binocular tube, or video-/phototube
(optional)
8
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Filter sets for Leica MZ10 F
Filter sets
Designation
Excitation filter Barrier filter
GFP fluorescence
GFP Plus fluorescence
GFP plant fluorescence
UV fluorescence
GFP1
GFP2
GFP3
UV
425/60 nm
480/40 nm
470/40 nm
360/40 nm
425/40 nm
470/40 nm
546/10 nm
480 nm
510 nm
525/50
Filter set GFP for LEICA MZ10 F
Excitation Filter (425/60) Barrier Filter (480)
1 0 0 %
420 nm
475 nm
515 nm
590 nm
5 0 %
Violet fluorescence
Blue fluorescence
Green fluorescence
Filter carrier, empty
V
B
0
%
G
350nm 400nm
500nm
600nm
700nm Wavelength
Filter set GFP Plant for LEICA MZ10 F
Excitation Filter (470/40) Barrier Filter (525/50)
Filter set GFP Plus for LEICA MZ10 F
Excitation Filter (480/40) Barrier Filter (510)
1 0 0 %
1 0 0 %
5 0 %
5 0 %
0
%
0
%
350nm 400nm
500nm
600nm
700nm Wavelength
350nm 400nm
500nm
600nm
700nm Wavelength
Filter set UV for LEICA MZ10 F
Barrier Filter (420)
Filter set Violet for LEICA MZ10 F
Excitation Filter (425/40) Barrier Filter (475)
Excitation Filter (360/40)
1 0 0 %
1 0 0 %
5 0 %
5 0 %
0
%
0
%
350nm 400nm
500nm
600nm
700nm Wavelength
350nm 400nm
500nm
600nm
700nm Wavelength
Filter set Blue for LEICA MZ10 F
Excitation Filter (470/40) Barrier Filter (515)
Filter set Green for LEICA MZ10 F
Excitation Filter (546/10) Barrier Filter (590)
1 0 0 %
1 0 0 %
5 0 %
5 0 %
0
%
0
%
350nm 400nm
500nm
600nm
700nm Wavelength
350nm 400nm
500nm
600nm
700nm Wavelength
B
L
U
-
S
P
E
K
.
D
R
W
9
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Leica MZ10 F
5a.Lamp housing 106Z
Assembly
5b.Stray-light protection
6. Binocular tube
1. Incident- or transmitted-light base
2a.Manual focusing drive, or
7. UV protection screen with arm
8. 4 sets of filters
2b.Motorized focusing drive
3. Microscope carrier for Leica MZ10 F
4. Leica MZ10 F optics carrier with FLUOIII™ filter system
9. Light stop
10. Filter slide for an individually-selectable filter
11. Double-iris diaphragm (optional)
12. Trinoculartube
5a
5b
12
6
9
10
4
8
2b
3
7
2a
1
10
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UV protection screen ➜ microscope carrier
Leica MZ10 F
Focusing drive ➜ base of stand
▶ Connect manually-operated or motor-driven focusing drive,
complete with column, to base, as described in user manual
M2-105-0EN.
Before using the motor focus, you must read the safety
directions in the accompanying user manual M2-267-
104.
▶ Using a hollow screw, fit the UV protection screen to the
microscope carrier on either the left or the right.
Microscope carrier ➜ focusing drive
▶ Fit microscope carrier of Leica MZ10 F to focusing drive in
Always position the UV protection screen so that
the user can never look directly at the light spot
(see page 4):
accordance with user manual M2-105-0EN.
Optics carrier ➜ microscope carrier
▶ Fit the UV protection screen
The Leica MZ10 F optics carrier and the FLUOIII filter
system form a single unit which was factory-adjusted. Do not
try to dismantle it.
with arm, at the side.
▶ Fit the Leica MZ10 F optics carrier to the microscope
carrier in accordance with the user manual M2-105-0EN.
Additional components
▶ Release the hollow screw.
▶ Fit the remaining components, such as binocular tube, eyepi-
eces and optional double-iris diaphragm, to the FLUOIII filter
system in accordance with user manual M2-105-0EN.
▶ Adjust the UV protection
screen, upwards
or downwards.
▶ Tighten the hollow screw.
Video-/phototube
To permit shorter exposure times in fluorescence photography,
we recommend you to use the video-/phototube HDV with 100%
light in the video-/photo beam path.
Double-iris diaphragm
The double-iris diaphragm is used to increase the depth of field
(see user manual M2-105-0EN).
▶ Fit the double-iris diaphragm to the FLUOIII filter system.
11
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▶ Slacken the Phillips
Assembly
screws
▶ Carefully extract the sok-
ket, complete
Lamp housing
The Leica 106Z lamp housing is equipped.
For information about the supply units for the 106Z lamp
housing, see page 14.
with burner mount and
connecting cable
Model 105Z lamp housings, if available, can also be used.
▶ Slacken the slit-headed screws
Observe the safety regulations on page 5
Notes and recommendations
▶ Remove the transport peg and keep it for subsequent
transport
▶ Dispose of old mercury-vapour burners in accordance
with environmental regulations.
•
•
•
•
•
Do not switch on and off more than necessary, because
this will shorten the burner life
Hot mercury-vapour burners cannot be ignited again until
after they have cooled
If possible, burn-in new mercury-vapour burners by running
them continuously for one or two hours
Be very careful when fitting the burner.
Do not apply pressure, as it breaks easily
Never touch the glass envelope of the burner with your fin-
gers. Fingerprints later burn in and reduce the light quality.
If necessary, carefully remove fingerprints and dust using a
cloth damped with alcohol and then wipe dry.
•
When a new mercury-vapour burner is fitted, the inscription
on the metal mount must be upright.
▶ Carefully insert the mercury-vapour burner into the lower
mount
▶ For mercury-vapour burners with seal point: Turn these so
Fitting and changing the mercury-vapour burner
that the seal point is at the side of the beam path
For the l06Z lamp housing, use the 100W mercury-vapour burner ▶ Carefully position the flexible power cable on the upper
type 307-072.057 or the 50W burner.
metal mount of the mercury-vapour burner.
The mercury-vapour burner is supplied in a separate package.
▶ Tighten the upper and
–
Units which are unmarked or are marked L1 are for lower
currents and higher voltages.
lower screws with great
care
–
Units marked L2 are for higher currents and lower voltages
(see page 14).
▶ Slacken the two Phillips
screws
▶ Pull the disconnect plug
slightly out
▶ Introduce the burner
mount, complete
without turning it
with burner, into the rail
of the lamp housing.
▶ Tighten the screws
▶ Flip open the cover
12
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▶ Using the focusing knob,
Removing the collector and the heat-protection filter
displace the collector:
The collector and the heat-protection filter can be removed
for cleaning or if defective.
•
During this movement, the
collector must not touch
the flexible power cable. If
necessary, bend the cable
away
▶ Open the lamp housing as described on page 12 and remove
the burner socket
▶ Move the reflector fully to
the right-hand stop
▶ Carefully close the lamp housing, making sure that
the disconnect plug engages the socket
▶ Press in the disconnect plug slightly
▶ Tighten the screws
▶ Push the disconnect plug fully in to the stop
without turning it.
▶ Hold the collector and pull
out the focusing knob
▶ Extract the collector
▶ Slacken the two screws
and remove the heat-
protection filter from the
collector
Lamp housing assemble
▶ Using the Allen key, release
the hollow screw
▶ Screw a new heat-
protection filter into
position
▶ Introduce the lamp housing
into the adapter of the
fluorescence module and
tighten the hollow screw.
Replacing the collector
▶ Pull out the focusing knob
Stray-light protection
▶ Carefully replace the collector and move it to the left until
There are slots in the lamp housing through which UV light can
shine on the user's hands during manipulations beneath the
housing. The stray-light protection on the underside of the lamp
housing is designed to block this light.
the focusing knob engages the guide groove
▶ Test the displacement of the collector
▶ Refit the burner socket (page 12) and close the lamp housing.
▶ Secure the stray-light
protection with two screws.
13
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Assembly
3
4
5
Supply unit for lamp housing 106Z
with 50W mercury-vapour burner
1. On/off switch
2. Running-time meter
3. Selector for L1/L2 burners
4. Fuse
5. Selector for 50Hz/60Hz
6. Connector for lamp
7. Power cable
2
1
6
7
The supply unit is intended for power supplies above 220V.
To run it on lower voltages a step-down transformer is required
(e.g. 230V to 110V).
Input type
220V/240V ±10%
220V ~50/60Hz
Hg50W L1/L2
Osram no. 0-958
Max. power 320VA
Observe the safety regulations on page 5.
▶ Connect the cable from the lamp housing to the supply unit
▶ Engage “L1” or “L2” in accordance with the inscription
on the burner socket (see page 12)
▶ Engage 50Hz or 60Hz
▶ Connect the power cable to the supply unit and to the grid
▶ Make a note of the running-time meter reading.
1
4
2 5
Supply unit for lamp housing 106Z
with 100W mercury-vapour burner
1. On/off switch
2. Pilot lamp LAMP
3
3. Pilot lamp TEMP
4. Running-time meter
5. Pilot lamp SAFETY
Observe the safety regulations on page 5
Type
ebq 100dc
Input voltage
Input frequency
Protection class
Series no.
90V to 250V AC, max 265VA
48Hz to 63 Hz
IP20
▶ Connect the cable from the lamp housing to the supply unit
▶ Secure the lamp cable.
G 34240090
▶ Connect the power cable to the supply unit and to the grid
▶ Make a note of the running-time meter reading.
Fuses
The fuse holder is secured in the holder with spring clips
on both sides.
▶ Insert a screwdriver behind the spring clips and extract
the fuse holder.
▶ Insert the fuses (2× T3,15A H250V F1/F2).
▶ Refit the fuse holder
14
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Adjusting the mercury vapour lamp
•
The discharge arc is visible in the illuminated spot and lies
at about 45° to the lines of the cross (fig. a)
This adjustment is important for obtaining a uniform light spot
and good-quality fluorescence.
▶ Look into the eyepieces and focus on the cross
▶ Using your unaided eye, observe the discharge arc on the
While adjusting the mirror image, do not project light
on to the electrodes for long periods (risk of explosion
through overheating)
paper and bring it into focus with the focusing knob (3) (fig. a)
▶ Using the positioning knobs (1 and 2), displace the discharge
arc (fig. b)
The two electrodes are imaged as an extension of the plane
of symmetry of the discharge arc, but are difficult to see.
▶ Bring the mirror image of the discharge arc into focus
with the knob (6) and use the knobs 4 and 5 to position it
symmetrically relative to the original image (fig. c)
▶ Switch on the supply unit and wait for two or three minutes
•
The 100W supply unit emits a whistle
•
For the 50W burner, the discharge-arc image and its
mirror image should touch
▶ Swing out the UV-light excluder (page 21)
•
Work without the UV-light excluder is only permitted for adju-
stment, and then only for short periods
•
For the 100W burner, the discharge-arc image and its mirror
image should be superimposed.
▶ Pull out the light stop (see page 16, MZ10 F and page 22,
▶ Using the focusing knob (3), readjust the illuminated field
Fluorescence module).
•
The illuminated field should now be large, circular and
as uniform as possible
▶ MZ10 F: Turn the filter set in the beam path (page 16).
▶ Stereo-fluorescence module: Insert the excitation filter
▶ Reposition the UV-light excluder correctly (page 21).
(page 22).
▶ Mark a cross on a piece of paper and place it in the middle
of the illuminated spot
▶ Select the lowest magnification
Hg 50W
Hg 100W
3
Figs. a: Bringing the discharge arc
into focus
1
2
Figs. b: Positioning the discharge arc
relative to the cross
4
5
6
Figs. c: Focusing and positioning the
mirror image of the discharge arc
15
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Dummy filter carrier
Leica MZ10 F
In the dummy filter carrier there are two openings for the
observation beam paths instead of the barrier filters. The third
path has been closed. Use the dummy filter carrier:
FLUOIII filter system
The filter system consists of a rapid filter changer for the
barrier- and excitation filters and two slots for a light stop and
for a filter slide respectively.
–
if you are using less than four sets of filters
–
if you are working without the fluorescence illuminator for
periods of up to 15 seconds.
Rapid filter changer
•
Do not use the dummy filter carrier to block the light from the
mercury-vapour burner for more than 15 seconds, or it will
heat up. Use the light stop instead.
The rapid filter changer of the Leica MZ10 F accepts a total of
four sets of filters. These are inscribed (see table on page 9).
Each filter set consists of two barrier filters for the observation
beam paths and one excitation filter for the illumination beam
path.
To avoid fingerprints on the filters in the filter sets, try to avoid
touching them. If fingerprints do occur, remove them with a
clean lint-free cloth and pure alcohol.
Light stop
The FLUOIII filter system includes a slot for the light stop. In
phases where the object is to be examined in e.g. transmitted
light instead of under conditions of fluorescence, the light stop
is used to block the illumination beam path so that the mercury-
vapour burner does not have its life shortened by being switched
on and off more than is absolutely necessary (see page 5).
An empty filter carrier is available for individual filter
combinations.
▶ Insert a set of filters into the rapid filter changer so that the
inscription (e.g. GFP) is upright and on the right-hand side.
Make sure that the shape of the filter set follows the shape
of the instrument.
Filter slide
Next to the light stop is a slot accepting a filter slide which
can hold an individually-selectable filter, e.g. a grey filter.
▶ Turn the filter set until it
engages.
▶ Insert a total of four sets of
filters, or insert dummy
filter carriers if the number
is less.
If you use less than four filter sets, you must insert in
the free spaces the dummy filter carriers provided.
If you leave spaces unfilled, you risk eye damage from
direct UV radiation from the third beam path
(see page 5).
Working without the fluorescence illuminator
▶ Use the light stop to block
the light from the
fluorescence illuminator.
▶ Turn the dummy filter carrier in the observation beam
path so that there is no barrier filter in position which might
cause false colour.
16
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▶ Illuminate a flat test object with transmitted light
Use
or with oblique incident light.
▶ Carry out the dioptric correction as directed in the user
MZ10 F: Bringing the specimen into focus and observing it
manual for the stereomicroscope.
Observe the safety directions on page 4 and the recom-
mendations on page 5 regarding the use of the mercu-
ry-vapour lamp.
Observing fluorescence
Make sure that the UV protection screen is positioned
correctly (page 11).
Changing filters
In the rapid filter changer, all four positions must be occupied,
either with filter sets or with dummy filter carriers (see page 16).
The active filter set is always the one with its inscription on the
left.
▶ Turn the sets of filters
until the inscription on the
one required is visible on
the left.
▶ Turn the required
filter set into the beam
path (page 16).
▶ Switch on the supply unit (pages 14) and wait for two or
three minutes.
▶ Pull out the light stop
(page 16).
Dioptric correction
We recommend you to carry out the dioptric correction either in
transmitted light or in incident light:
▶ If the fluorescence illumi-
nator is switched on, use
the light stop to block the
light (page 16).
▶ Using the lowest magnification, observe the object.
•
This will give you a better overall view and you will be able to
locate features of interest more easily.
▶ Refocus slightly if necessary.
▶ Observe the details at higher magnification.
Reflexes (hot-spots)
When the 1.6× planapochromatic objective is used at zoom
positions between 0.8 and 1.6, a slight reflex (hot-spot) occurs
in the lower part of the field of view; it disappears at higher zoom
positions. This reflex does not arise in conjunction with the
1.0× and 0.5× planapochromatic objectives or the achromatic
objectives.
▶ Swing the dummy filter
carrier into the beam path
(page 16).
17
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Overview
Outfit with stereo-fluorescence module
5a Leica 106Z lamp housing for 50W or 100W mercury-vapour
burners
The components
1
Choice of stand and of focusing drive
(manual or motor-driven)
5b Stray-light protection
6
Supply unit for 50W or 100W mercury-vapour burner
(not illustrated)
2
3
Microscope carrier
Leica MS5, MZ6, MZ7.5 – 16 optics carrier
7
8
9
UV-light excluder with arm and clamp
Double-iris diaphragm (optional)
4a Leica stereo-fluorescence module with choice of barrier fil-
ter
Choice of binocular tube, or optional video-/phototube
4b Filter insert for excitation filter
4c Light stop
4d Filter holder with an individually-selectable filter
4e Adapter for light source
5a, 5b
9
4e
4a
8
4b, 4c, 4d
3
2
6
7
1
18
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Filter sets for stereo-fluorescence module
Filter sets
Excitation
filters
Dichromatic
Barrier
beam splitters filters
GFP fluorescence
GFP-Plus fluorescence
GFP-Plant
425/60nm
480/40nm
470/40nm
360/40nm
425/40nm
470/40nm
546/10nm
470nm
505nm LP
495nm
400nm
460nm
505nm
565nm
GG475
510nm LP
525/50nm
GG420
UV fluorescence
Violet fluorescence
Blue fluorescence
Green fluorescence
GG475
OG515
OG590
19
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Assembly
Stereo-fluorescence module
7 Binocular tube
8 Clamp
1 Incident- or transmitted-light base
2a Manual focusing drive, or
2b Motorized focusing drive
3 Microscope carrier
9 Arm
10 UV-light excluder
11 Filter carrier
12 Filterholder for excitation filter
13 Light stop
4 Choice of optics carrier
5 Fluorescence module
6a 106Z lamp housing
14 Filter carrier with an individually-selectable filter
15 Double-iris diaphragm (optional)
16 HU video-/phototube (optional)
6b Stray-light protection
6a
6b
7
15
16
13,14
12
11
5
4
8
9
10
20
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Stereo-fluorescence module
Focusing drive ➜ base of stand
▶ Connect manually-operated or motor-driven focusing drive,
complete with column, to base, as described in user manual
M2-105-0EN.
3
Before using the motor focus, you must read
the safety directions in the accompanying user
manual M2-267-104.
Important: With the illuminator on, insert the filterhol-
der with diaphragm into slide-in unit 3 to avoid being
suddenly exposed to excessively bright light (page 22).
▶ Remove the binocular tube.
▶ Loosen the 3 socket-head
screws.
▶ Remove the cover.
For the Leica stereomicroscope models MS5, MZ6, MZ8, MZ12,
MZAPO with manual focusing drive, fit the microscope in
the upper position on the focusing drive (refer to user manual for
stereomicroscope).
▶ Loosen the fastening
screw and take out the
filter carrier.
Fluorescence module ➜ stereomicroscope
•
Assemble in the following order without double-iris dia-
phragm: Optics carrier, fluorescence module, binocular tube
or phototube
▶ Insert the desired filter
carrier. Fit both of the
two guide pins in the
fluorescence module into
the holes on the filter
carrier.
•
Assemble in the following order with double-iris diaphragm:
Optics carrier, double-iris diaphragm, fluorescence module,
binocular tube or phototube
▶ Align the fluorescence module on the optics carrier so that
the adapter for the lamp housing points backwards at
about 45°
▶ Tighten the fastening
screw securely.
▶ Continue assembling in accordance with the user manual for
▶ Replace the cover and
tighten the 3 socket-head
screws securely.
the stereomicroscope (see section “Fitting accessory tubes”)
Video-/phototubes
The fluorescence illumination is directed along the left-hand
beam path and could cause hot-spots in the image. Therefore:
▶ Position the HU phototube over the right-hand beam path.
▶ Replace the binocular tube.
▶ Insert a suitable exciter filter (page 22).
▶ Take out the filterholder with diaphragm.
▶ Re-adjust the light source (page 15).
Changing the filter set
Removable filter sets that can hold various filters are available for
the fluorescence module. A filter set consists of a filter carrier
with barrier filter and of a filterholder with excitation filter.
If you wish to change the filter set while you are in the middle
of a task, leave the illuminator switched on so that you will
not have to wait for the lamp to cool (page 12) before continuing.
21
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Filterholder ➜ fluorescence module
Assembly
Stereo-fluorescence module
The dichromatic beam splitter and the barrier filter are built into
the fluorescence module in accordance with the configuration
(see table, page 19).
The excitation filter is supplied in a separate filterholder.
UV-light excluder ➜ side-faced column
A filterholder with diaphragm is also supplied. This diaphragm
can be slid into the illumination beam path if the object should
not be illuminated.
Always position the UV-light excluder so that the user
never looks directly at the light spot (page 5).
▶ Using the clamping screw,
secure the clamp on
the side-faced column,
beneath the focusing drive.
3
2
1
The filter slide on the fluorescence module has space
for three filterholders:
▶ Slacken the central fixing
screw.
•
•
•
with excitation filter (holder 1)
▶ Unfold the arm.
with diaphragm (holder 3)
▶ Tighten the central fixing
with individually-selectable filter, e.g. neutral filter
(holder 2).
screw.
▶ Secure the arm, com-
plete with connector, to
the clamp, on the left or
right. Tighten the clamping
screw.
Double-iris diaphragm
The double-iris diaphragm is used to increase the depth of field
(see user manual M2-105-0EN). Also, the stray reflections arising
in the left-hand beam path can be minimized with the help of
the double-iris diaphragm.
The HU video-/phototube already includes a double-iris
diaphragm.
▶ Secure the UV-light
excluder to the arm.
Tighten the clamping
screw.
Additional assembly instructions
Lamp housing
Supply units
pages 12–13
page 14
Adjusting the mercury-vapour lamp page 15
22
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Observation
Use
▶ First use the lowest magnification to observe the object
Stereo-fluorescence module
•
You will gain a better overall view and can more easily find
interesting features
Focusing and observing
▶ Refocus slightly if necessary
Observe the safety regulations on page 4 and note the
recommendations on page 5 for using the mercury-
vapour burner. Position the UV-light excluder correctly
(see page 22):
▶ Observe details at a higher magnification.
▶ Hold the arm
▶ Release the central
clamping screw
▶ Position the UV-light
excluder in front of the
objective
▶ Tighten the central
clamping screw.
Focusing
Carry out the dioptric correction and the focusing in accordance
with the user manual for the stereomicroscope, either:
in transmitted light:
▶ pushing in the filter holder with diaphragm (3) to block the
fluorescence illumination (see page 22). Pull the filter holder
with diaphragm out again before observing the fluorescence.
3
with the fluorescence illumination:
▶ pulling out the filter holder with diaphragm (3)
(see page 22)
▶ Insert the excitation filter (1) (page 22)
3
2
1
▶ Switch on the supply unit (page 14
). For the 100W lamp,
wait two or three minutes for the whistle signal.
23
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Leica Microsystems – the brand
for outstanding products
Leica Microsystems’ mission is to be the world’s first-choice provider of innova-
tive solutions to our customers’ needs for vision, measurement and analysis of
micro-structures.
The companies of the Leica Micro-
systems Group operate international-
ly in three business segments, where
we rank with the market leaders.
Leica, the leading brand for microscopes and scientific instruments, developed
from five brand names, all with a long tradition: Wild, Leitz, Reichert, Jung and
Cambridge Instruments. Yet Leica symbolizes innovation as well as tradition.
Microscopy Systems
•
Our expertise in microscopy is the
basis for all our solutions for visuali-
zation, measurement and analysis of
micro-structures in life sciences and
industry. With confocal laser techno-
logy and image analysis systems, we
provide three-dimensional viewing
facilities and offer new solutions for
cytogenetics, pathology and materi-
als sciences.
Leica Microsystems – an international company
with a strong network of customer services.
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Specimen Preparation
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We provide comprehensive systems
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