Leica Microscope Magnifier 106Z User Manual

Leica Stereo-  
Fluorescence  
Systems  
User Manual  
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Contents  
Page  
Leica MZ10 F  
fluorescence stereomicroscope  
Description. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4  
Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 4  
Fluorescence applications . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 6  
Components, controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 8  
Sets of filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 9  
Assembly  
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 10  
Focusing drive, microscope carrier, MZ10 F optics carrier . . . . . . . . . . . . . . . . . . . 11  
UV protection screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 11  
Model 106Z lamp housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12  
Mercury-vapour lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12  
Stray-light protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13  
Collector, heat-protection filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13  
Supply units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14  
Adjusting the mercury-vapour lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15  
Operation  
FLUOIII™ filter system, light stop . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 16  
Adjusting the dioptric setting, focusing, observing . . . . . . . . . . . . . . . . . . . . . . . . . . 17  
Leica stereo-fluorescence module  
Components, controls . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18  
Sets of filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 19  
Assembly  
Overview . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 20  
Focusing drive, microscope carrier, optics carrier . . . . . . . . . . . . . . . . . . . . . . . . . . 21  
Fluorescence module . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21  
Sets of filters . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 21  
UV protection screen. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 22  
Model 106Z lamp housing . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12  
Mercury-vapour lamps . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 12  
Stray-light protection . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13  
Collector, heat-protection filter . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 13  
Supply units . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14  
Adjusting the mercury-vapour lamp . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 15  
Operation  
Filter changer, light stop, double-iris diaphragm . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23  
Adjusting the dioptric setting, focusing, observing . . . . . . . . . . . . . . . . . . . . . . . . . . 23  
3
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Description  
Safety concept  
Before you set up the instrument and before you fit the  
fluorescence illuminator, read:  
Fluorescence technique  
this user manual, observing the notes relating to safety  
Some substances fluoresce when irradiated with short-wave  
light. This property is utilized in the fluorescence technique,  
in which certain structures and features which do not fluo-  
rescence can be tagged with a fluorescing dye. An example  
is green-fluorescing protein (GFP), which is used in molecular  
biology.  
the user manual for your stereomicroscope, observing the  
notes relating to safety and care.  
Permitted uses  
Leica stereo-fluorescence systems are equipped with a special  
fluorescence illuminator. They are intended for the three-  
dimensional observation of fluorescing objects. They consist of:  
Stereo-fluorescence  
With Leica fluorescence systems you can complete unprepared  
fluorescing specimens to be non-destructively studied in three  
dimensions, manipulated, sorted and recorded. The intense light  
emitted by the mercury-vapour burner, used in conjunction with  
selected filter sets, enables even the finest structures to be  
differentiated and expands the amount of information revealed  
by the incident-light fluorescence technique.  
a stereomicroscope with stand, binocular tube and  
eyepieces  
an integrated FLUOIII filter system (for MZ10 F)  
or a separate stereo-fluorescence module  
appropriate filter sets with barrier- and excitation filters,  
a light stop, and individually-selectable filters  
a UV protection screen  
Fluorescence stereomicroscope  
a lamp housing with high-pressure mercury vapour burner  
a supply unit with power cable.  
The Leica MZ10 F with 10:1 zoom is the routine stereo-  
microscope for fluorescence applications. The patented sepa-  
rate beam path (TriBeam™) for the fluorescence illuminator and  
the patented filter system (FLUOIII™) together produce excellent  
fluorescence images.  
Prohibited uses  
The use of Leica stereo-fluorescence systems in a different  
manner from that described in this user manual can lead to inju-  
ry, malfunction and damage.  
Stereo-fluorescence module  
The stereo-fluorescence modul can be combined with the  
Leica stereomicroscope models MS5, MZ6, MZ7.5, MZ9.5,  
MZ12.5, MZ16, and with older models.  
Do not fit different plugs or cables  
Do not dismantle or modify components unless instructions  
for doing so are given in the user manual  
Components may only be opened by authorized personnel.  
User manual  
The present user manual describes only the functions of the  
fluorescence illuminator, the use of the filter sets, and the fitting  
of the fluorescence module. For detailed information about the  
use of the stereomicroscope, its care and its safety, please refer  
to those sections of the separate manual M2-105-0EN which  
relate to the Leica MZ12.5 with 12.5:1 zoom.  
Place of use  
Leica stereo-fluorescence systems are intended for use in  
closed rooms and may not be used outdoors.  
Responsibilities of person in charge of instrument  
Ensure that the Leica stereo-fluorescence systems are  
operated, maintained and repaired only by authorized and  
trained personnel  
Ensure that personnel who use the instrument have read  
and understood this user manual and in particular all safety  
instructions.  
4
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Supply unit  
Servicing and repairs  
Pull the power plug of the supply unit out from the power sok-  
ket:  
Repairs may only be carried out by Leica-trained service  
technicians or by technical personnel authorized to do so by  
the person in charge of the instrument  
when assembling and dismantling the lamp housing  
before opening the lamp housing  
Only original Leica spare parts may be used  
Unplug the power cable before opening the supply unit.  
Touching live circuits can cause injury.  
when changing the mercury-vapour burner and other  
components such as the heat-protection filter or the collector  
when servicing the supply unit  
Legal requirements  
Adhere to general and local regulations relating to accident  
prevention and environmental protection.  
Lamp housing  
Never open the lamp housing while the burner is switched on  
(risk of explosion, UV irradiation, dazzling)  
Conformity with European Community directive  
Leica stereo-fluorescence systems and their accessories are  
constructed in accordance with the latest technologies and are  
provided with a statement of conformity with EC requirements.  
Always allow the lamp housing to cool for at least 15 minutes  
before opening it (risk of burner exploding)  
Never cover the ventilator slots on the lamp housing (risk of fire)  
Mercury-vapour lamp  
Read the user manual and safety directions provided by the  
manufacturer of the mercury-vapour burner, and particularly  
those relating to its breakage and to the release of mercury  
Before transporting the equipment, remove the mercury-vapour  
burner and place it in its original packaging. Use the transport  
peg in place of the burner, to secure movable parts within the  
lamp housing  
Light source: Safety regulations  
Safety measures introduced by manufacturer  
When the mercury-vapour burner has reached the end of its  
nominal working life as indicated by the manufacturer’s  
information and the time counter on the supply unit, change the  
discoloured burner (increased risk of explosion)  
UV protection screen in front of the specimen plane prevents  
direct UV radiation from reaching the eyes.  
Dummy filter carriers in the unoccupied positions of the rapid  
filter changer prevent direct UV radiation from reaching  
the eyes  
Leica declines all responsibility for injury and damage  
resulting from exploding, incorrectly-fitted or incorrectly-used  
mercury-vapour burners.  
UV filters in the observation beam paths protect the eyes  
against UV radiation.  
Stray-light protection on the underside of the lamp housing  
protects the user's hands against UV radiation.  
Warning  
UV can damage your eyes. Therefore:  
Never look at the light spot on the specimen plane without  
the UV protection screen in position  
Never look into the eyepieces unless there is an excitation  
filter in the beam path  
Always keep dummy filter carriers in the unoccupied  
positions of the rapid filter changer (for MZ10 F)  
Do not place the specimen on a white or highly-reflective  
background.  
5
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Applications  
Natural science  
Application  
Fluorescence filter set  
Anatomy  
Biology  
• Monitoring of capillary flow  
• GFP 1, GFP 2  
• GFP 1, GFP 2  
• Gene expression in chicken embryos, fruit flies,  
threadworms and zebra fish  
• Fish otoliths marked with alizarine red  
• Genetic expression through viruses and bacteria  
which infect plants and animals  
• Green  
• GFP 2  
Biomedicine  
Genetics  
• Humatic seals on pacemakers  
• Blue, green  
• GFP 1, GFP 2  
• Cellular detection and protein expression  
• Sorting and dissection  
• Monitoring developmental processes  
Neurology  
• Gap junctions on muscles and nerves  
• Cell development in rats' eyes  
• GFP 1, violet  
• GFP 1, GFP 2  
Ophthalmology  
Pharmacy  
• Drugs  
• GFP 1, GFP 2  
• Green  
• GFP 2  
• ELI spotting in cell structures  
• Monitoring of capillary flow with FITC  
Plants and seeds  
• Plants, genetic expression, transgenetics  
• Bacteria detection  
• GFP 1, GFP 2  
• GFP 3  
Botany  
• Plant cells, plant surfaces, soil samples  
Hydrology  
• Water quality (bacterial and other pollutants)  
• Filtered water  
• Cell structures in and on the filter membrane  
• UV  
• GFP 1  
• GFP 1  
Parasitology  
Forestry  
• Detection of bacteria on ticks  
• GFP 1, GFP 2  
• Development of environmentally-acceptable methods of pest control • GFP 1  
(investigation of viruses on pests)  
6
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Industry  
Application  
Fluorescence filter set  
Electronics  
• Solder paste on SMDs  
• Epoxy resin on SMD plates  
• Luminescent coating of TV monitor tubes  
• Quality of polymer castings for embedding integrated circuits  
• GFP 1, GFP 2  
• GFP 1, GFP 2  
• GFP 1, GFP 2  
• Violet  
Semiconductors  
• Positive photo resists (OCG HPR 504)  
• Negative photo resists  
• GFP 1, green  
• Blue  
• Detection of foreign particles on masks, wafers and  
• subassemblies  
• UV, GFP 1, GFP 2  
• Violet  
Petrochemicals  
Polymers  
• Oil inspection  
• GFP 1  
• Detection of foreign particles  
• Blue  
• UV  
• GFP 1, GFP 2  
• Identification of non-polymerized parts  
• Examination of beads (polymer pellets used in chemical  
measurements and analyses)  
Engineering  
• Inspection of cemented areas on mechanical  
or optical components  
• GFP 1, violet  
• GFP 1  
Metalworking  
industries  
• Cracks and surface defects  
• Detection of contamination on components  
• Quality control of welds  
• Fracture analysis  
Materials science  
• Cracks, fractures, welds  
• Carbon bonding materials; fractures and orientation  
of carbon fibres  
• GFP 1  
• Violet  
Bitumen  
• Quality control for tar and bitumen  
• Cracks and pores  
• GFP 1  
• GFP 1  
Concrete  
Papermaking  
• Coating of paper fibres  
• Investigation of inclusions  
• GFP 1  
• Violet  
Forensic work  
• Textile fibres, fingerprints, banknotes,  
forgeries, body fluids  
• GFP 1, GFP 2  
Art restoration  
Gemmology  
• Pigments, forgeries  
• UV  
Quality, value, inclusions  
• Blue  
7
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Overview  
3e  
4a  
3c  
Leica MZ10 F: Its components and controls  
1. Choice of stand and of focusing drive  
8
(manual or motor-driven)  
2. Microscope carrier for Leica MZ10 F  
3a. Leica MZ10 F optics carrier with third beam path  
3b. Integrated FLUOIII filter system  
3b  
3d  
3c. Adapter for light source  
3d. Rapid filter changer for any of four sets of filters  
4b  
3a  
2
3e. Slots for light stop and for filter slide with  
individually-selectable filter  
4a. Leica 106Z lamp housing for 50W or 100W  
high-pressure mercury vapour burners  
6
4b. Stray-light protection  
5. Supply units for 106Z lamp housing (not illustrated)  
6. UV protection screen with arm  
7. Double-iris diaphragm (optional)  
1
8. Choice of binocular tube, or video-/phototube  
(optional)  
8
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Filter sets for Leica MZ10 F  
Filter sets  
Designation  
Excitation filter Barrier filter  
GFP fluorescence  
GFP Plus fluorescence  
GFP plant fluorescence  
UV fluorescence  
GFP1  
GFP2  
GFP3  
UV  
425/60 nm  
480/40 nm  
470/40 nm  
360/40 nm  
425/40 nm  
470/40 nm  
546/10 nm  
480 nm  
510 nm  
525/50  
Filter set GFP for LEICA MZ10 F  
Excitation Filter (425/60) Barrier Filter (480)  
1 0 0 %  
420 nm  
475 nm  
515 nm  
590 nm  
5 0 %  
Violet fluorescence  
Blue fluorescence  
Green fluorescence  
Filter carrier, empty  
V
B
0
%
G
350nm 400nm  
500nm  
600nm  
700nm Wavelength  
Filter set GFP Plant for LEICA MZ10 F  
Excitation Filter (470/40) Barrier Filter (525/50)  
Filter set GFP Plus for LEICA MZ10 F  
Excitation Filter (480/40) Barrier Filter (510)  
1 0 0 %  
1 0 0 %  
5 0 %  
5 0 %  
0
%
0
%
350nm 400nm  
500nm  
600nm  
700nm Wavelength  
350nm 400nm  
500nm  
600nm  
700nm Wavelength  
Filter set UV for LEICA MZ10 F  
Barrier Filter (420)  
Filter set Violet for LEICA MZ10 F  
Excitation Filter (425/40) Barrier Filter (475)  
Excitation Filter (360/40)  
1 0 0 %  
1 0 0 %  
5 0 %  
5 0 %  
0
%
0
%
350nm 400nm  
500nm  
600nm  
700nm Wavelength  
350nm 400nm  
500nm  
600nm  
700nm Wavelength  
Filter set Blue for LEICA MZ10 F  
Excitation Filter (470/40) Barrier Filter (515)  
Filter set Green for LEICA MZ10 F  
Excitation Filter (546/10) Barrier Filter (590)  
1 0 0 %  
1 0 0 %  
5 0 %  
5 0 %  
0
%
0
%
350nm 400nm  
500nm  
600nm  
700nm Wavelength  
350nm 400nm  
500nm  
600nm  
700nm Wavelength  
B
L
U
-
S
P
E
K
.
D
R
W
9
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Leica MZ10 F  
5a.Lamp housing 106Z  
Assembly  
5b.Stray-light protection  
6. Binocular tube  
1. Incident- or transmitted-light base  
2a.Manual focusing drive, or  
7. UV protection screen with arm  
8. 4 sets of filters  
2b.Motorized focusing drive  
3. Microscope carrier for Leica MZ10 F  
4. Leica MZ10 F optics carrier with FLUOIII™ filter system  
9. Light stop  
10. Filter slide for an individually-selectable filter  
11. Double-iris diaphragm (optional)  
12. Trinoculartube  
5a  
5b  
12  
6
9
10  
4
8
2b  
3
7
2a  
1
10  
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UV protection screen microscope carrier  
Leica MZ10 F  
Focusing drive base of stand  
Connect manually-operated or motor-driven focusing drive,  
complete with column, to base, as described in user manual  
M2-105-0EN.  
Before using the motor focus, you must read the safety  
directions in the accompanying user manual M2-267-  
104.  
Using a hollow screw, fit the UV protection screen to the  
microscope carrier on either the left or the right.  
Microscope carrier focusing drive  
Fit microscope carrier of Leica MZ10 F to focusing drive in  
Always position the UV protection screen so that  
the user can never look directly at the light spot  
(see page 4):  
accordance with user manual M2-105-0EN.  
Optics carrier microscope carrier  
Fit the UV protection screen  
The Leica MZ10 F optics carrier and the FLUOIII filter  
system form a single unit which was factory-adjusted. Do not  
try to dismantle it.  
with arm, at the side.  
Fit the Leica MZ10 F optics carrier to the microscope  
carrier in accordance with the user manual M2-105-0EN.  
Additional components  
Release the hollow screw.  
Fit the remaining components, such as binocular tube, eyepi-  
eces and optional double-iris diaphragm, to the FLUOIII filter  
system in accordance with user manual M2-105-0EN.  
Adjust the UV protection  
screen, upwards  
or downwards.  
Tighten the hollow screw.  
Video-/phototube  
To permit shorter exposure times in fluorescence photography,  
we recommend you to use the video-/phototube HDV with 100%  
light in the video-/photo beam path.  
Double-iris diaphragm  
The double-iris diaphragm is used to increase the depth of field  
(see user manual M2-105-0EN).  
Fit the double-iris diaphragm to the FLUOIII filter system.  
11  
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Slacken the Phillips  
Assembly  
screws  
Carefully extract the sok-  
ket, complete  
Lamp housing  
The Leica 106Z lamp housing is equipped.  
For information about the supply units for the 106Z lamp  
housing, see page 14.  
with burner mount and  
connecting cable  
Model 105Z lamp housings, if available, can also be used.  
Slacken the slit-headed screws  
Observe the safety regulations on page 5  
Notes and recommendations  
Remove the transport peg and keep it for subsequent  
transport  
Dispose of old mercury-vapour burners in accordance  
with environmental regulations.  
Do not switch on and off more than necessary, because  
this will shorten the burner life  
Hot mercury-vapour burners cannot be ignited again until  
after they have cooled  
If possible, burn-in new mercury-vapour burners by running  
them continuously for one or two hours  
Be very careful when fitting the burner.  
Do not apply pressure, as it breaks easily  
Never touch the glass envelope of the burner with your fin-  
gers. Fingerprints later burn in and reduce the light quality.  
If necessary, carefully remove fingerprints and dust using a  
cloth damped with alcohol and then wipe dry.  
When a new mercury-vapour burner is fitted, the inscription  
on the metal mount must be upright.  
Carefully insert the mercury-vapour burner into the lower  
mount  
For mercury-vapour burners with seal point: Turn these so  
Fitting and changing the mercury-vapour burner  
that the seal point is at the side of the beam path  
For the l06Z lamp housing, use the 100W mercury-vapour burner Carefully position the flexible power cable on the upper  
type 307-072.057 or the 50W burner.  
metal mount of the mercury-vapour burner.  
The mercury-vapour burner is supplied in a separate package.  
Tighten the upper and  
Units which are unmarked or are marked L1 are for lower  
currents and higher voltages.  
lower screws with great  
care  
Units marked L2 are for higher currents and lower voltages  
(see page 14).  
Slacken the two Phillips  
screws  
Pull the disconnect plug  
slightly out  
Introduce the burner  
mount, complete  
without turning it  
with burner, into the rail  
of the lamp housing.  
Tighten the screws  
Flip open the cover  
12  
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Using the focusing knob,  
Removing the collector and the heat-protection filter  
displace the collector:  
The collector and the heat-protection filter can be removed  
for cleaning or if defective.  
During this movement, the  
collector must not touch  
the flexible power cable. If  
necessary, bend the cable  
away  
Open the lamp housing as described on page 12 and remove  
the burner socket  
Move the reflector fully to  
the right-hand stop  
Carefully close the lamp housing, making sure that  
the disconnect plug engages the socket  
Press in the disconnect plug slightly  
Tighten the screws  
Push the disconnect plug fully in to the stop  
without turning it.  
Hold the collector and pull  
out the focusing knob  
Extract the collector  
Slacken the two screws  
and remove the heat-  
protection filter from the  
collector  
Lamp housing assemble  
Using the Allen key, release  
the hollow screw  
Screw a new heat-  
protection filter into  
position  
Introduce the lamp housing  
into the adapter of the  
fluorescence module and  
tighten the hollow screw.  
Replacing the collector  
Pull out the focusing knob  
Stray-light protection  
Carefully replace the collector and move it to the left until  
There are slots in the lamp housing through which UV light can  
shine on the user's hands during manipulations beneath the  
housing. The stray-light protection on the underside of the lamp  
housing is designed to block this light.  
the focusing knob engages the guide groove  
Test the displacement of the collector  
Refit the burner socket (page 12) and close the lamp housing.  
Secure the stray-light  
protection with two screws.  
13  
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Assembly  
3
4
5
Supply unit for lamp housing 106Z  
with 50W mercury-vapour burner  
1. On/off switch  
2. Running-time meter  
3. Selector for L1/L2 burners  
4. Fuse  
5. Selector for 50Hz/60Hz  
6. Connector for lamp  
7. Power cable  
2
1
6
7
The supply unit is intended for power supplies above 220V.  
To run it on lower voltages a step-down transformer is required  
(e.g. 230V to 110V).  
Input type  
220V/240V ±10%  
220V ~50/60Hz  
Hg50W L1/L2  
Osram no. 0-958  
Max. power 320VA  
Observe the safety regulations on page 5.  
Connect the cable from the lamp housing to the supply unit  
Engage “L1” or “L2” in accordance with the inscription  
on the burner socket (see page 12)  
Engage 50Hz or 60Hz  
Connect the power cable to the supply unit and to the grid  
Make a note of the running-time meter reading.  
1
4
2 5  
Supply unit for lamp housing 106Z  
with 100W mercury-vapour burner  
1. On/off switch  
2. Pilot lamp LAMP  
3
3. Pilot lamp TEMP  
4. Running-time meter  
5. Pilot lamp SAFETY  
Observe the safety regulations on page 5  
Type  
ebq 100dc  
Input voltage  
Input frequency  
Protection class  
Series no.  
90V to 250V AC, max 265VA  
48Hz to 63 Hz  
IP20  
Connect the cable from the lamp housing to the supply unit  
Secure the lamp cable.  
G 34240090  
Connect the power cable to the supply unit and to the grid  
Make a note of the running-time meter reading.  
Fuses  
The fuse holder is secured in the holder with spring clips  
on both sides.  
Insert a screwdriver behind the spring clips and extract  
the fuse holder.  
Insert the fuses (2× T3,15A H250V F1/F2).  
Refit the fuse holder  
14  
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Adjusting the mercury vapour lamp  
The discharge arc is visible in the illuminated spot and lies  
at about 45° to the lines of the cross (fig. a)  
This adjustment is important for obtaining a uniform light spot  
and good-quality fluorescence.  
Look into the eyepieces and focus on the cross  
Using your unaided eye, observe the discharge arc on the  
While adjusting the mirror image, do not project light  
on to the electrodes for long periods (risk of explosion  
through overheating)  
paper and bring it into focus with the focusing knob (3) (fig. a)  
Using the positioning knobs (1 and 2), displace the discharge  
arc (fig. b)  
The two electrodes are imaged as an extension of the plane  
of symmetry of the discharge arc, but are difficult to see.  
Bring the mirror image of the discharge arc into focus  
with the knob (6) and use the knobs 4 and 5 to position it  
symmetrically relative to the original image (fig. c)  
Switch on the supply unit and wait for two or three minutes  
The 100W supply unit emits a whistle  
For the 50W burner, the discharge-arc image and its  
mirror image should touch  
Swing out the UV-light excluder (page 21)  
Work without the UV-light excluder is only permitted for adju-  
stment, and then only for short periods  
For the 100W burner, the discharge-arc image and its mirror  
image should be superimposed.  
Pull out the light stop (see page 16, MZ10 F and page 22,  
Using the focusing knob (3), readjust the illuminated field  
Fluorescence module).  
The illuminated field should now be large, circular and  
as uniform as possible  
MZ10 F: Turn the filter set in the beam path (page 16).  
Stereo-fluorescence module: Insert the excitation filter  
Reposition the UV-light excluder correctly (page 21).  
(page 22).  
Mark a cross on a piece of paper and place it in the middle  
of the illuminated spot  
Select the lowest magnification  
Hg 50W  
Hg 100W  
3
Figs. a: Bringing the discharge arc  
into focus  
1
2
Figs. b: Positioning the discharge arc  
relative to the cross  
4
5
6
Figs. c: Focusing and positioning the  
mirror image of the discharge arc  
15  
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Dummy filter carrier  
Leica MZ10 F  
In the dummy filter carrier there are two openings for the  
observation beam paths instead of the barrier filters. The third  
path has been closed. Use the dummy filter carrier:  
FLUOIII filter system  
The filter system consists of a rapid filter changer for the  
barrier- and excitation filters and two slots for a light stop and  
for a filter slide respectively.  
if you are using less than four sets of filters  
if you are working without the fluorescence illuminator for  
periods of up to 15 seconds.  
Rapid filter changer  
Do not use the dummy filter carrier to block the light from the  
mercury-vapour burner for more than 15 seconds, or it will  
heat up. Use the light stop instead.  
The rapid filter changer of the Leica MZ10 F accepts a total of  
four sets of filters. These are inscribed (see table on page 9).  
Each filter set consists of two barrier filters for the observation  
beam paths and one excitation filter for the illumination beam  
path.  
To avoid fingerprints on the filters in the filter sets, try to avoid  
touching them. If fingerprints do occur, remove them with a  
clean lint-free cloth and pure alcohol.  
Light stop  
The FLUOIII filter system includes a slot for the light stop. In  
phases where the object is to be examined in e.g. transmitted  
light instead of under conditions of fluorescence, the light stop  
is used to block the illumination beam path so that the mercury-  
vapour burner does not have its life shortened by being switched  
on and off more than is absolutely necessary (see page 5).  
An empty filter carrier is available for individual filter  
combinations.  
Insert a set of filters into the rapid filter changer so that the  
inscription (e.g. GFP) is upright and on the right-hand side.  
Make sure that the shape of the filter set follows the shape  
of the instrument.  
Filter slide  
Next to the light stop is a slot accepting a filter slide which  
can hold an individually-selectable filter, e.g. a grey filter.  
Turn the filter set until it  
engages.  
Insert a total of four sets of  
filters, or insert dummy  
filter carriers if the number  
is less.  
If you use less than four filter sets, you must insert in  
the free spaces the dummy filter carriers provided.  
If you leave spaces unfilled, you risk eye damage from  
direct UV radiation from the third beam path  
(see page 5).  
Working without the fluorescence illuminator  
Use the light stop to block  
the light from the  
fluorescence illuminator.  
Turn the dummy filter carrier in the observation beam  
path so that there is no barrier filter in position which might  
cause false colour.  
16  
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Illuminate a flat test object with transmitted light  
Use  
or with oblique incident light.  
Carry out the dioptric correction as directed in the user  
MZ10 F: Bringing the specimen into focus and observing it  
manual for the stereomicroscope.  
Observe the safety directions on page 4 and the recom-  
mendations on page 5 regarding the use of the mercu-  
ry-vapour lamp.  
Observing fluorescence  
Make sure that the UV protection screen is positioned  
correctly (page 11).  
Changing filters  
In the rapid filter changer, all four positions must be occupied,  
either with filter sets or with dummy filter carriers (see page 16).  
The active filter set is always the one with its inscription on the  
left.  
Turn the sets of filters  
until the inscription on the  
one required is visible on  
the left.  
Turn the required  
filter set into the beam  
path (page 16).  
Switch on the supply unit (pages 14) and wait for two or  
three minutes.  
Pull out the light stop  
(page 16).  
Dioptric correction  
We recommend you to carry out the dioptric correction either in  
transmitted light or in incident light:  
If the fluorescence illumi-  
nator is switched on, use  
the light stop to block the  
light (page 16).  
Using the lowest magnification, observe the object.  
This will give you a better overall view and you will be able to  
locate features of interest more easily.  
Refocus slightly if necessary.  
Observe the details at higher magnification.  
Reflexes (hot-spots)  
When the 1.6× planapochromatic objective is used at zoom  
positions between 0.8 and 1.6, a slight reflex (hot-spot) occurs  
in the lower part of the field of view; it disappears at higher zoom  
positions. This reflex does not arise in conjunction with the  
1.0× and 0.5× planapochromatic objectives or the achromatic  
objectives.  
Swing the dummy filter  
carrier into the beam path  
(page 16).  
17  
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Overview  
Outfit with stereo-fluorescence module  
5a Leica 106Z lamp housing for 50W or 100W mercury-vapour  
burners  
The components  
1
Choice of stand and of focusing drive  
(manual or motor-driven)  
5b Stray-light protection  
6
Supply unit for 50W or 100W mercury-vapour burner  
(not illustrated)  
2
3
Microscope carrier  
Leica MS5, MZ6, MZ7.5 – 16 optics carrier  
7
8
9
UV-light excluder with arm and clamp  
Double-iris diaphragm (optional)  
4a Leica stereo-fluorescence module with choice of barrier fil-  
ter  
Choice of binocular tube, or optional video-/phototube  
4b Filter insert for excitation filter  
4c Light stop  
4d Filter holder with an individually-selectable filter  
4e Adapter for light source  
5a, 5b  
9
4e  
4a  
8
4b, 4c, 4d  
3
2
6
7
1
18  
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Filter sets for stereo-fluorescence module  
Filter sets  
Excitation  
filters  
Dichromatic  
Barrier  
beam splitters filters  
GFP fluorescence  
GFP-Plus fluorescence  
GFP-Plant  
425/60nm  
480/40nm  
470/40nm  
360/40nm  
425/40nm  
470/40nm  
546/10nm  
470nm  
505nm LP  
495nm  
400nm  
460nm  
505nm  
565nm  
GG475  
510nm LP  
525/50nm  
GG420  
UV fluorescence  
Violet fluorescence  
Blue fluorescence  
Green fluorescence  
GG475  
OG515  
OG590  
19  
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Assembly  
Stereo-fluorescence module  
7 Binocular tube  
8 Clamp  
1 Incident- or transmitted-light base  
2a Manual focusing drive, or  
2b Motorized focusing drive  
3 Microscope carrier  
9 Arm  
10 UV-light excluder  
11 Filter carrier  
12 Filterholder for excitation filter  
13 Light stop  
4 Choice of optics carrier  
5 Fluorescence module  
6a 106Z lamp housing  
14 Filter carrier with an individually-selectable filter  
15 Double-iris diaphragm (optional)  
16 HU video-/phototube (optional)  
6b Stray-light protection  
6a  
6b  
7
15  
16  
13,14  
12  
11  
5
4
8
9
10  
20  
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Stereo-fluorescence module  
Focusing drive base of stand  
Connect manually-operated or motor-driven focusing drive,  
complete with column, to base, as described in user manual  
M2-105-0EN.  
3
Before using the motor focus, you must read  
the safety directions in the accompanying user  
manual M2-267-104.  
Important: With the illuminator on, insert the filterhol-  
der with diaphragm into slide-in unit 3 to avoid being  
suddenly exposed to excessively bright light (page 22).  
Remove the binocular tube.  
Loosen the 3 socket-head  
screws.  
Remove the cover.  
For the Leica stereomicroscope models MS5, MZ6, MZ8, MZ12,  
MZAPO with manual focusing drive, fit the microscope in  
the upper position on the focusing drive (refer to user manual for  
stereomicroscope).  
Loosen the fastening  
screw and take out the  
filter carrier.  
Fluorescence module stereomicroscope  
Assemble in the following order without double-iris dia-  
phragm: Optics carrier, fluorescence module, binocular tube  
or phototube  
Insert the desired filter  
carrier. Fit both of the  
two guide pins in the  
fluorescence module into  
the holes on the filter  
carrier.  
Assemble in the following order with double-iris diaphragm:  
Optics carrier, double-iris diaphragm, fluorescence module,  
binocular tube or phototube  
Align the fluorescence module on the optics carrier so that  
the adapter for the lamp housing points backwards at  
about 45°  
Tighten the fastening  
screw securely.  
Continue assembling in accordance with the user manual for  
Replace the cover and  
tighten the 3 socket-head  
screws securely.  
the stereomicroscope (see section “Fitting accessory tubes”)  
Video-/phototubes  
The fluorescence illumination is directed along the left-hand  
beam path and could cause hot-spots in the image. Therefore:  
Position the HU phototube over the right-hand beam path.  
Replace the binocular tube.  
Insert a suitable exciter filter (page 22).  
Take out the filterholder with diaphragm.  
Re-adjust the light source (page 15).  
Changing the filter set  
Removable filter sets that can hold various filters are available for  
the fluorescence module. A filter set consists of a filter carrier  
with barrier filter and of a filterholder with excitation filter.  
If you wish to change the filter set while you are in the middle  
of a task, leave the illuminator switched on so that you will  
not have to wait for the lamp to cool (page 12) before continuing.  
21  
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Filterholder fluorescence module  
Assembly  
Stereo-fluorescence module  
The dichromatic beam splitter and the barrier filter are built into  
the fluorescence module in accordance with the configuration  
(see table, page 19).  
The excitation filter is supplied in a separate filterholder.  
UV-light excluder side-faced column  
A filterholder with diaphragm is also supplied. This diaphragm  
can be slid into the illumination beam path if the object should  
not be illuminated.  
Always position the UV-light excluder so that the user  
never looks directly at the light spot (page 5).  
Using the clamping screw,  
secure the clamp on  
the side-faced column,  
beneath the focusing drive.  
3
2
1
The filter slide on the fluorescence module has space  
for three filterholders:  
Slacken the central fixing  
screw.  
with excitation filter (holder 1)  
Unfold the arm.  
with diaphragm (holder 3)  
Tighten the central fixing  
with individually-selectable filter, e.g. neutral filter  
(holder 2).  
screw.  
Secure the arm, com-  
plete with connector, to  
the clamp, on the left or  
right. Tighten the clamping  
screw.  
Double-iris diaphragm  
The double-iris diaphragm is used to increase the depth of field  
(see user manual M2-105-0EN). Also, the stray reflections arising  
in the left-hand beam path can be minimized with the help of  
the double-iris diaphragm.  
The HU video-/phototube already includes a double-iris  
diaphragm.  
Secure the UV-light  
excluder to the arm.  
Tighten the clamping  
screw.  
Additional assembly instructions  
Lamp housing  
Supply units  
pages 12–13  
page 14  
Adjusting the mercury-vapour lamp page 15  
22  
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Observation  
Use  
First use the lowest magnification to observe the object  
Stereo-fluorescence module  
You will gain a better overall view and can more easily find  
interesting features  
Focusing and observing  
Refocus slightly if necessary  
Observe the safety regulations on page 4 and note the  
recommendations on page 5 for using the mercury-  
vapour burner. Position the UV-light excluder correctly  
(see page 22):  
Observe details at a higher magnification.  
Hold the arm  
Release the central  
clamping screw  
Position the UV-light  
excluder in front of the  
objective  
Tighten the central  
clamping screw.  
Focusing  
Carry out the dioptric correction and the focusing in accordance  
with the user manual for the stereomicroscope, either:  
in transmitted light:  
pushing in the filter holder with diaphragm (3) to block the  
fluorescence illumination (see page 22). Pull the filter holder  
with diaphragm out again before observing the fluorescence.  
3
with the fluorescence illumination:  
pulling out the filter holder with diaphragm (3)  
(see page 22)  
Insert the excitation filter (1) (page 22)  
3
2
1
Switch on the supply unit (page 14  
). For the 100W lamp,  
wait two or three minutes for the whistle signal.  
23  
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Leica Microsystems – the brand  
for outstanding products  
Leica Microsystems’ mission is to be the world’s first-choice provider of innova-  
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micro-structures.  
The companies of the Leica Micro-  
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Leica, the leading brand for microscopes and scientific instruments, developed  
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Our expertise in microscopy is the  
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