Leica Computer Accessories NCL TSH User Manual

NovocastraLyophilized  
Mouse Monoclonal Antibody  
Thyroid Stimulating Hormone  
Product Code: NCL-TSH  
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.  
Intended Use  
Thyroid stimulating hormone (TSH). No reactivity with luteinising hormone, follicle stimulating  
hormone and human chorionic gonadotrophin.  
Specificity  
QB2/6  
Clone  
IgG1  
Ig Class  
TSH molecule.  
Antigen Used for  
Immunizations  
Mouse myeloma (NS0).  
Hybridoma Partner  
Lyophilized tissue culture supernatant containing sodium azide.  
Reconstitute with the volume of sterile distilled water indicated on the vial label.  
Preparation  
Yes.  
Yes.  
Effective on Frozen Tissue  
Effective on Paraffin Wax  
Embedded Tissue  
Immunohistochemistry on paraffin sections.  
Recommendations on Use  
Enzyme Induced Epitope Retrieval (EIER): Please follow the instructions for use in Novocastra  
Enzyme Proteinase K (IHC).  
Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should  
determine their own optimal working dilutions.  
Visualization: Please follow the instructions for use in the NovolinkPolymer Detection Systems.  
For further product information or support, contact your local distributor or regional office of Leica  
The performance of this antibody should be validated when utilized with other manual staining  
systems or automated platforms.  
Immunohistochemistry: Normal human pituitary gland.  
Cytoplasmic  
Positive Controls  
Staining Pattern  
Store unopened lyophilized antibody at 2-8 °C. Under these conditions, there is no significant  
loss in product performance up to the expiry date indicated on the vial label. The reconstituted  
antibody is stable for at least two months when stored at 2-8 °C. For long term storage, it is  
recommended that aliquots of the antibody are frozen at -20 °C (frost-free freezers are not  
recommended). Repeated freezing and thawing must be avoided. Prepare working dilutions on  
the day of use.  
Storage and Stability  
This reagent has been prepared from the supernatant of cell culture. As it is a biological product,  
reasonable care should be taken when handling it. This reagent contains sodium azide. A Material  
Warnings and Precautions  
Leica Biosystems Newcastle Ltd  
Balliol Business Park West  
Benton Lane  
Newcastle Upon Tyne NE12 8EW  
United Kingdom  
TSH 27/07/2012  
© Leica Biosystems Newcastle Ltd • NCL- Rev C  
(
+44 191 215 4242  
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