Hach Scanner DR 5000 User Manual
DOC022.53.00654  
DR 5000  
USER MANUAL  
January 2008, Edition 2  
© HACH Company, 2007–2008. All rights reserved. Printed in Germany.  
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Table of Contents  
Section 1 Specifications ........................................................................................................................................ 7  
Section 2 General Information .............................................................................................................................. 9  
2.1 Safety information .............................................................................................................................................. 9  
2.1.1 Use of hazard information ......................................................................................................................... 9  
2.1.2 Precautionary labels ................................................................................................................................. 9  
2.1.3 Class 1 LASER ....................................................................................................................................... 10  
2.1.4 Chemical and Biological Safety .............................................................................................................. 10  
2.1.5 Source Lamp Safety ............................................................................................................................... 10  
2.2 Overview of product ......................................................................................................................................... 11  
Section 3 Installation ........................................................................................................................................... 13  
3.1 Unpack the instrument ..................................................................................................................................... 13  
3.2 Operating environment .................................................................................................................................... 13  
3.3 Connection ....................................................................................................................................................... 14  
3.4 Cell compartment, Multi Cell Holder, 100 mm Cell Adapter (optional) ............................................................. 15  
3.4.1 100 mm adapter (optional) ...................................................................................................................... 16  
3.4.2 Installation Multi Cell Holder ................................................................................................................... 17  
3.5 Beam path ........................................................................................................................................................ 18  
Section 4 Start Up ................................................................................................................................................ 19  
4.1 Power the instrument on and off ...................................................................................................................... 19  
4.2 Language selection .......................................................................................................................................... 19  
4.3 Self-Check ....................................................................................................................................................... 19  
Section 5 Standard Operations ........................................................................................................................... 21  
5.1 Overview .......................................................................................................................................................... 21  
5.1.1 Tips for the use of the touch screen ........................................................................................................ 21  
5.1.2 Use of the alphanumeric keypad ............................................................................................................ 21  
5.1.3 Main Menu .............................................................................................................................................. 22  
5.2 Instrument Setup mode .................................................................................................................................... 23  
5.2.1 Operator ID ............................................................................................................................................. 23  
5.2.2 Sample ID ............................................................................................................................................... 24  
5.2.3 Date and time .......................................................................................................................................... 25  
5.2.4 Display and sound preferences .............................................................................................................. 25  
5.2.5 Lamp Control .......................................................................................................................................... 26  
5.2.5.1 VIS-Lamp and UV-Lamp ............................................................................................................. 26  
5.2.5.2 Effect caused by the lamp settings on the Measuring Mode ...................................................... 27  
5.2.6 PC and printer ......................................................................................................................................... 29  
5.2.6.1 Printer setup ............................................................................................................................... 29  
5.2.6.2 Print data .................................................................................................................................... 31  
5.2.6.3 HACH Data Trans ....................................................................................................................... 31  
5.2.7 Password ................................................................................................................................................ 32  
5.2.7.1 Password deactivation ................................................................................................................ 33  
5.3 Store, recall, send and delete data .................................................................................................................. 35  
5.3.1 The Data Log .......................................................................................................................................... 35  
5.3.1.1 Auto/manual data storage ........................................................................................................... 35  
5.3.1.2 Recall stored data from the data log ........................................................................................... 35  
5.3.1.3 Send data from the data log ....................................................................................................... 36  
5.3.1.4 Delete stored data from the data log .......................................................................................... 37  
5.3.2 Store, recall, send and delete data from wavelength scan and time course ........................................... 38  
5.3.2.1 Data storage from wavelength scan or time course ................................................................... 38  
5.3.2.2 Recall stored data from wavelength scan or time course ........................................................... 38  
5.3.2.3 Send data from wavelength scan or time course ........................................................................ 39  
5.3.2.4 Delete stored data from wavelength scan or time course ........................................................... 41  
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Table of Contents  
5.4 Stored Programs .............................................................................................................................................. 42  
5.4.1 Select a saved test/method; enter user-specific basic data .................................................................... 42  
5.4.2 Stored program options .......................................................................................................................... 42  
5.4.3 Use of program timers ............................................................................................................................ 44  
5.4.4 Set the reading mode .............................................................................................................................. 44  
5.4.4.1 Take single wavelength measurements (single reading) ............................................................ 45  
5.4.4.2 Take single wavelength measurements (continuous readings) .................................................. 45  
5.4.5 Set the dilution factor .............................................................................................................................. 45  
5.4.6 Run a standard adjust ............................................................................................................................. 46  
5.4.7 Set the chemical form ............................................................................................................................. 47  
5.4.7.1 Change of the default setting of the chemical form .................................................................... 47  
5.4.8 Run a reagent blank ................................................................................................................................ 48  
5.4.9 Analysis of samples ................................................................................................................................ 49  
5.4.10 Add stored programs to the favorite programs list ................................................................................ 49  
5.5 Barcode Programs ........................................................................................................................................... 50  
5.5.1 Complete a barcode 13 mm test/vial ...................................................................................................... 51  
5.5.2 Select the measuring range .................................................................................................................... 51  
5.5.3 Select the chemical evaluation form ....................................................................................................... 51  
5.5.3.1 Change of the default setting of the chemical form .................................................................... 52  
5.5.4 Basic test-specific and sample-specific data settings ............................................................................. 52  
5.5.5 Sample blank .......................................................................................................................................... 53  
5.5.6 Update/edit barcode tests ....................................................................................................................... 54  
5.5.6.1 Manual update of a barcode test ................................................................................................ 54  
5.5.6.2 Update an existing barcode test ................................................................................................. 57  
5.5.6.3 Program a new test ..................................................................................................................... 58  
5.5.7 Upgrade of the instrument software ........................................................................................................ 58  
Section 6 Advanced Operations ......................................................................................................................... 61  
6.1 User Programs ................................................................................................................................................. 61  
6.1.1 Program a user method .......................................................................................................................... 61  
6.1.1.1 Single wavelength settings ......................................................................................................... 63  
6.1.1.2 Multi wavelength settings ............................................................................................................ 64  
6.1.1.3 Calibration settings for single and multi wavelength mode ......................................................... 66  
6.1.1.4 Store a user program .................................................................................................................. 70  
6.1.1.5 Additional user-defined parameters and functions ..................................................................... 70  
6.1.2 Free programming program type ............................................................................................................ 72  
6.1.2.1 Measurement process ................................................................................................................ 72  
6.1.2.2 Enter a new element of a measuring sequence ......................................................................... 73  
6.1.2.3 Enter the calibration formula (evaluation formula) ...................................................................... 75  
6.1.2.4 Enter variables ............................................................................................................................ 78  
6.1.2.5 Save a free programming user program ..................................................................................... 78  
6.1.3 Select a user program ............................................................................................................................. 79  
6.1.4 Add, edit and delete user programs from the favorites list ...................................................................... 79  
6.1.4.1 Add to Favorites .......................................................................................................................... 80  
6.1.4.2 Edit .............................................................................................................................................. 80  
6.1.4.3 Delete ......................................................................................................................................... 80  
6.2 Favorite Programs ........................................................................................................................................... 81  
6.2.1 Recall a favorite program ........................................................................................................................ 81  
6.2.2 Delete a favorite program ....................................................................................................................... 81  
6.3 Standard Addition – monitoring/checking results ............................................................................................. 82  
6.3.1 Complete a standard addition ................................................................................................................. 83  
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Table of Contents  
6.4 Single Wavelength (absorbance, concentration and transmittance measurements) ....................................... 87  
6.4.1 Set up single wavelength mode .............................................................................................................. 87  
6.4.2 Take single wavelength measurements (single reading) ........................................................................ 89  
6.4.3 Take single wavelength measurements (continuous readings) .............................................................. 89  
6.5 Multi-Wavelength mode – measurements at more than one wavelength ........................................................ 90  
6.5.1 Set the reading mode at different wavelengths ....................................................................................... 90  
6.5.2 Complete a measurement in the multi wavelength mode ....................................................................... 93  
6.6 Wavelength Scan mode – recording of absorbance and transmittance spectrums ......................................... 94  
6.6.1 Set up the wavelength scan .................................................................................................................... 94  
6.6.2 Wavelength scan reading ....................................................................................................................... 97  
6.6.2.1 Navigation of the wavelength scan graph or a wavelength scan analysis .................................. 98  
6.6.3 Work with reference scans ...................................................................................................................... 99  
6.7 Time course of absorbance/transmittance ..................................................................................................... 101  
6.7.1 Time course setup parameters ............................................................................................................. 101  
6.7.2 Time course scan reading ..................................................................................................................... 102  
6.7.3 Analysis of time course data ................................................................................................................. 103  
6.7.3.1 Navigation of a time scan or a time scan analysis .................................................................... 104  
6.8 System checks ............................................................................................................................................... 105  
6.8.1 Instrument information .......................................................................................................................... 105  
6.8.2 Upgrade of the instrument software ...................................................................................................... 106  
6.8.3 Optical checks ....................................................................................................................................... 106  
6.8.3.1 Wavelength check .................................................................................................................... 107  
6.8.3.2 Noise check .............................................................................................................................. 108  
6.8.3.3 Stray light check ....................................................................................................................... 110  
6.8.3.4 Absorbance check .................................................................................................................... 111  
6.8.3.5 Drift check ................................................................................................................................. 112  
6.8.3.6 Verification kit ........................................................................................................................... 113  
6.8.4 Output checks ....................................................................................................................................... 115  
6.8.5 Lamp history ......................................................................................................................................... 115  
6.8.6 Factory service ...................................................................................................................................... 116  
6.8.7 Service time .......................................................................................................................................... 116  
6.8.8 Instrument Backup ................................................................................................................................ 117  
Section 7 Maintenance ...................................................................................................................................... 121  
7.1 Cleaning requirements ................................................................................................................................... 121  
7.1.1 Spectrophotometer ............................................................................................................................... 121  
7.1.2 Display .................................................................................................................................................. 121  
7.1.3 Cuvettes/sample cells ........................................................................................................................... 121  
7.2 Lamp replacement ......................................................................................................................................... 122  
7.2.1 Tungsten lamp replacement ................................................................................................................. 122  
7.2.2 Deuterium lamp (UV) replacement ....................................................................................................... 123  
7.3 Fuse replacement .......................................................................................................................................... 124  
7.4 Filter pad maintenance .................................................................................................................................. 125  
7.4.1 Filter pad replacement .......................................................................................................................... 125  
Section 8 Troubleshooting ................................................................................................................................ 127  
Section 9 Replacement Parts ............................................................................................................................ 129  
Section 10 Contact Information ........................................................................................................................ 131  
Section 11 Limited Warranty ............................................................................................................................. 133  
Index .................................................................................................................................................................... 135  
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Table of Contents  
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Section 1 Specifications  
Specifications are subject to change without notice.  
Performance specifications  
Operating mode  
Transmittance (%), Absorbance and Concentration  
Source lamp  
Gas-filled Tungsten (visible) and Deuterium (UV)  
Wavelength range  
190–1100 nm  
Wavelength accuracy  
Wavelength reproducibility  
Wavelength resolution  
Wavelength calibration  
Wavelength selection  
Scanning speed  
± 1 nm in Wavelength Range 200–900 nm  
< 0.1 nm  
0.1 nm  
Automatic  
Automatic, based on method selection  
900 nm/min 1 nm steps  
2 nm  
Spectral bandwidth  
Photometric range  
± 3.0 Abs in Wavelength Range 200–900 nm  
5 mAbs at 0.0–0.5 Abs  
1% at 0.50–2.0 Abs  
Photometric accuracy  
Photometric linearity  
Stray light  
< 0.5%–2 Abs  
< = 1% at > 2 Abs  
with neutral glass at 546 nm  
KI-solution at 220 nm  
> 3.3 Abs / < 0.05%  
Data storage  
1000 measured values (result, date, time, sample ID, user ID)  
50  
User programs  
Physical and environmental specifications  
Width  
450 mm (17.7 in)  
Height  
200 mm (7.9 in)  
Depth  
500 mm (19.7 in)  
Weight  
15.5 kg (34.2 Ibs)  
Operating requirements  
Storage requirements  
Additional technical data  
Mains connection  
10–40 °C (50–104 °F), max. 80% relative humidity (non-condensing)  
–25–60 °C (–13–140 °F) max. 80% relative humidity (non-condensing)  
100–240 V~; 50/60 Hz; automatic changeover  
Use only shielded cable with max. length of 3 m.  
1 x USB type B  
Interfaces  
2 x USB type A  
Water-resistant to  
Enclosure  
IP 31  
Safety class  
Safety class I  
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Specifications  
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Section 2 General Information  
2.1 Safety information  
Please read this entire manual before unpacking, setting up or  
operating this equipment. Pay attention to all danger, warning and  
caution statements. Failure to do so could result in serious injury to  
the operator or damage to the equipment.  
Make sure that the protection provided by this equipment is not  
impaired, do not use or install this equipment in any manner other  
than that specified in this manual.  
2.1.1 Use of hazard information  
DANGER  
Indicates a potentially or imminently hazardous situation  
which, if not avoided, will result in death or serious injury.  
WARNING  
Indicates a potentially or imminently hazardous situation  
which, if not avoided, could result in death or serious injury.  
CAUTION  
Indicates a potentially hazardous situation that may result in  
minor or moderate injury.  
Important Note: Indicates a situation which, if not avoided, may  
cause damage to the instrument. Information that requires special  
emphasis.  
Note: Information that supplements points in the main text.  
2.1.2 Precautionary labels  
Read all labels and tags attached to the instrument. Personal injury  
or damage to the instrument could occur if not observed. A symbol,  
if noted on the instrument, will be included with a danger or caution  
statement in the manual.  
This symbol, if noted on the instrument, references the instruction manual for operation and/or safety information.  
This symbol, if noted on the instrument, indicates a hot surface.  
Electrical equipment marked with this symbol may not be disposed of in European public disposal systems after  
12 August of 2005. In conformity with European local and national regulations (EU Directive 2002/96/EC),  
European electrical equipment users must now return old or end-of life equipment to the Producer for disposal at no  
charge to the user.  
Note: For return for recycling, please contact the equipment producer or supplier for instructions on how to return  
end-of-life equipment, producer-supplied electrical accessories and all auxiliary items for proper disposal.  
This symbol indicates that the instrument contains a Class 1 LASER device.  
LASER CLASS 1  
Data: 0.3 mW; λ = 650 nm  
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General Information  
2.1.3 Class 1 LASER  
A Class 1 LASER is installed in this instrument. Class 1 LASERS  
are products where the radiant power of the LASER beam  
accessible (the accessible emission) is always below the Maximum  
Permissible Exposure value. Therefore, for Class 1 LASERS the  
output power is below the level at which it is believed eye damage  
will occur. Exposure to the beam of a Class 1 LASER will not result  
in eye injury. Class 1 LASERS may therefore be considered safe.  
However, Class 1 LASER products may contain LASER systems of  
a higher Class but there are adequate engineering control  
measures to ensure that access to the beam is not reasonably  
likely. Examples of such products include LASER printers and  
compact disc players. CDRH assession number 0510555-02.  
Data: 0.3 mW; wavelength = 650 nm  
2.1.4 Chemical and Biological Safety  
DANGER  
Potential Chemical/ Biological Exposure Hazards. Handling  
chemical samples, standards and reagents can be dangerous.  
Users of this product are advised to familiarize themselves  
with safety procedures and the correct use of chemicals, and  
to carefully read all relevant Material Safety Data Sheets.  
Normal operation of this instrument may involve the use of  
hazardous chemicals or biologically harmful samples.  
•
The user must observe all cautionary information printed on the  
original solution containers and safety data sheet prior to their  
use.  
•
•
All waste solutions must be disposed in accordance with local  
and national law.  
The type of protective equipment must be selected according to  
the concentration and amount of the dangerous substance at  
the specific workplace.  
2.1.5 Source Lamp Safety  
The source lamp operate at high temperatures.  
•
To avoid a possible electric shock, disconnect the instrument  
from the power source before servicing the lamp. Make sure  
that the instrument cannot be accidentally switched on again.  
CAUTION  
The UV lamp generates UV light.  
Do not look directly at an operating lamp without wearing UV  
protective eye glasses.  
Important Note: To prevent a possible burn, allow the lamp to cool  
at least 30 minutes before handling.  
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General Information  
2.2 Overview of product  
The DR 5000 Spectrophotometer is a complete scanning UV/VIS  
spectrophotometer with a wavelength range of 190 to 1100 nm. The  
instrument comes with a complete set of application programs and  
multi-language support.  
The DR 5000 Spectrophotometer contains the following application  
modes: Stored Programs, User Programs, Favorite Programs,  
Single Wavelength Mode, Multi-Wavelength Mode, Wavelength  
Scanning Mode and Time Course Mode.  
The DR 5000 is used for testing in visible and ultraviolet  
wavelengths. A gas-filled tungsten lamp produces light in the visible  
spectrum (320 to 1100 nm), and a deuterium lamp available  
produces light in the ultraviolet spectrum (190 to 360 nm).  
The DR 5000 Spectrophotometer provides digital readouts in direct  
concentration units, absorbance, or percent transmittance.  
When a user-generated or programmed method is selected, the  
on-screen menus and prompts direct the user through the test.  
This menu system also can be used to generate reports, statistical  
evaluations of generated calibration curves, and to report  
instrument diagnostic checks.  
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General Information  
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Section 3 Installation  
WARNING  
Electrical and Fire Hazards. Use only the provided power  
supply. Only qualified personnel should conduct the tasks  
described in this section of the manual.  
3.1 Unpack the instrument  
The DR 5000 Spectrophotometer comes packaged with the  
following items:  
•
•
•
•
•
DR 5000 Spectrophotometer  
Mains power cable  
Multi Cell Holder  
DR 5000 User Manual  
CD-ROM containing the HACH and HACH LANGE procedures  
Note: If any of these items are missing or damaged, contact the  
manufacturer or a sales representative immediatly.  
3.2 Operating environment  
The following conditions are necessary to ensure correct  
instrument operation and accurate results:  
•
•
•
•
•
•
Place the instrument firmly on an even surface. Do not push  
any objects under the instrument.  
Maintain an ambient temperature of 10 to 40 ºC (50 to 104 ºF)  
for proper instrument operation.  
The relative humidity should be less than 80%; moisture should  
not condense on the instrument.  
Leave at least a 15 cm (6 in.) clearance at the top and on all  
sides for air circulation to avoid overheating of electrical parts.  
Do not operate or store the instrument in extremely dusty,  
damp or wet locations.  
Keep the surface of the instrument, the cell compartment and  
all accessories clean and dry at all times. Splashes or spills on  
and in the instrument should be cleaned up immediately (see  
section 7.1 on page 121).  
Important Note: Protect the instrument from temperature  
extremes, including heaters, direct sunlight and other heat sources.  
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Installation  
3.3 Connection  
The DR 5000 has three USB interfaces as a standard feature,  
located on the back of the instrument (Figure 1).  
The USB Type A interfaces are used for communications with a  
printer, USB memory stick or keyboard. A USB memory stick is  
used to update instrument software.  
The USB Type B interface is used for communications with a PC.  
The optional Hach Data Trans software (see section 5.2.6.3 on  
page 31) must be installed on the PC for this use.  
A USB hub may be used to connect several accessories at a time.  
Note: USB cables must not be longer than 3 meters (10 feet).  
These USB interfaces enable data and graphics to be output to a  
Printer and a PC and upgrade of instrument software (see  
section 6.8.2 on page 106).  
Figure 1 Interfaces  
1
2
3
USB type A  
USB type B  
On/Off switch  
4
5
Fuse  
Plug in power supply  
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Installation  
3.4 Cell compartment, Multi Cell Holder, 100 mm Cell Adapter (optional)  
Figure 2 Cell compartment  
Cell compartment for 13 mm or 16 mm  
round cuvettes/vials only  
1
Cell compartment  
2
The DR 5000 Spectrophotometer comes equipped with a Multi Cell  
Holder (Figure 3 on page 16) which is the Standard Holder,  
provided with each instrument. The Multi Cell Holder can  
accommodate the following cell types:  
•
•
•
•
10 mm square cells  
20/50 mm rectangular cells  
1“ round cells  
1“ square cells  
Only one cell type can be used for a measurement. On the top and  
bottom of the Multi Cell Holder are a variety of openings to  
accommodate different types of cells. Beside each opening is  
printed the type of cell for which it is intended.  
Optionally there is a 100 mm adapter available.  
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Installation  
Figure 3 Multi Cell Holder (Top and Bottom)  
1
2
3
10 mm square cell  
4
5
1“ round cell  
20 mm rectangular cell  
50 mm rectangular cell  
1“ square cell  
3.4.1 100 mm adapter (optional)  
Figure 4 100 mm Cell Adapter  
1
100 mm rectangular cell  
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Installation  
3.4.2 Installation Multi Cell Holder  
Assembly of the Multi Cell Holder:  
1. Open the cell compartment.  
2. Identify the correct opening for the selected cell type in the  
Multi Cell Holder.  
3. Insert the Multi Cell Holder in the cell compartment in such a  
way that the name of the selected cell type can be read directly  
and the cell opening is at the front. Secure the holder with two  
locking screws (Figure 5).  
Figure 5 Installation Multi Cell Holder  
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Installation  
3.5 Beam path  
Figure 6 shows the beam path of the DR 5000:  
Figure 6 Beam path  
10 Exit slit  
1
2
3
4
5
6
7
8
9
Deuterium lamp  
Lamp selection mirror  
Tungsten lamp  
Mirror  
11 Cell compartment  
12 Lens  
13 Round cuvette/vial compartment  
14 Measurement element  
15 Monochromator  
16 Mirror  
Grating  
Reference element  
Mirror  
Splitter mirror  
Filter  
17 Mirror  
18 Entrance slit  
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Section 4 Start Up  
4.1 Power the instrument on and off  
1. Plug in power supply.  
2. Close the empty cell compartment.  
3. Turn the instrument on by pressing the power switch on the  
back.  
Note: Do not turn the instrument off and on in rapid succession. Always  
wait about 20 seconds before turning the instrument on again, otherwise  
the electronic and mechanical systems will be damaged.  
4.2 Language selection  
The spectrophotometer software includes several language  
options. The first time the instrument is turned on, the language  
selection screen will appear.  
1. Select the desired language.  
2. Press OK to confirm the language selection. The self check will  
start automatically.  
Changing the language setting  
The instrument functions in the selected language until the option is  
changed.  
1. While turning the instrument on, touch the screen at any point  
until the list for selecting a language appears (about  
30 seconds).  
2. Select the required language.  
3. Press OK to confirm. The test program subsequently starts  
automatically.  
4.3 Self-Check  
Each time the instrument is powered up, a series of diagnostic tests  
are performed automatically to ensure operation of major system  
components.  
This procedure, which takes approximately two minutes, checks the  
system, lamp, filter adjustment, wavelength calibration and voltage.  
Each test which functions correctly is confirmed with a check mark.  
The Main Menu is displayed when power up diagnostics are  
completed.  
Note: Further error messages during self check, see Section 8 on page  
127.  
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Start Up  
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Section 5 Standard Operations  
5.1 Overview  
5.1.1 Tips for the use of the touch screen  
The entire screen is touch-activated. To make a selection, press the  
screen with a fingernail, fingertip, pencil eraser or a stylus. Do not  
press the screen with a sharp object, such as the tip of a ball  
point pen.  
•
Do not place anything on top of the screen, to prevent damage  
or scratching on the screen.  
•
•
Press keys, words or icons to select them.  
Use scroll bars to move up and down long lists very quickly.  
Press and hold the scroll bar, then move up or down to move  
through the list.  
•
Highlight an item from a list by pressing it once. When the item  
has been successfully selected, it will be displayed as reversed  
text (light text on a dark background).  
5.1.2 Use of the alphanumeric keypad  
This display is used to enter letters, numbers and symbols as  
needed when programming the instrument. Unavailable options are  
disabled (grayed out). The icons on the right and left of the screen  
are described in Table 1.  
The central keypad changes to reflect the chosen entry mode.  
Press a key repeatedly until the desired character appears on the  
screen. A space can be entered by using the underscore on the  
YZ_ key.  
Note: A USB keyboard (with US keyboard layout) or a USB Barcode  
handset scanner can be used for input (see Section 9 on page 129).  
Table 1 Alphanumeric keypad  
Function  
Icon / key  
ABC/abc  
Description  
When entering alphabetic characters (ex. user-entered units), this key allows to  
toggle between upper and lower case letters.  
Alphabetic  
# %  
123  
CE  
Symbols  
Numeric  
Punctuation, symbols and numerical sub- and superscripts may be entered.  
For entering regular numbers.  
Clear Entry  
Clear the entry.  
Moves back one position. This deletes the character previously entered in the new  
position.  
Left Arrow  
Backspace  
Advance  
Moves to the next space in an entry when two adjacent characters occur on the  
same key.  
Right Arrow  
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Standard Operations  
5.1.3 Main Menu  
A variety of modes may be selected from the Main Menu. The  
following table briefly describes each menu option.  
Table 2 Main Menu options  
Option  
Function  
Stored programs are pre-programmed methods that make use of HACH reagents and LANGE  
Stored Programs (HACH cuvette tests and pipette tests.  
LANGE Programs)  
The DR 5000 Procedures Manual contains illustrated, step-by-step procedures for analyses  
using HACH programs. The working procedures for LANGE tests are included in the test packs.  
User programs make "made to measure analysis" possible:  
–Users can program methods they have developed themselves  
User Programs  
–Existing HACH and LANGE methods can be stored as user programs. The LANGE tests can  
then be modified to suit the user's requirements.  
Favorite Programs  
List of methods/tests created by the user to suit his own requirements.  
Single wavelength measurements are:  
Absorbance measurements: The light absorbed by the sample is measured in absorbance  
units.  
Single Wavelength  
Transmittance measurements (%): The percentage of the light that passes through the sample  
and reaches the detector is measured.  
Concentration measurements: A concentration factor can be entered to enable the measured  
absorbance values to be converted into concentration values.  
In the multi-wavelength mode, absorbance (Abs) or percentage transmittance (%T) is measured  
at up to four wavelengths and absorbance differences and absorbance relationships are  
calculated. Simple conversions into concentrations can also be carried out.  
Multi Wavelength  
Wavelength Scan  
A wavelength scan shows how the light from a sample is absorbed over a defined wavelength  
spectrum.This function can be used to determine the wavelength at which the maximum  
absorbance value can be measured.The absorbance behavior is displayed graphically during the  
scan.  
Time Course  
The time scan records the absorbance or % transmittance at a wavelength over a defined time.  
The system checks menu offers a number of options, including optical checks, output checks,  
lamp history, instrument update, service time and instrument backup.  
System Checks  
Recall Data  
Stored data can be recalled, filtered, sent and deleted.  
In this mode, user-specific or method-specific settings can be entered: Operator-ID, Sample-ID,  
Date & Time, Display & Sound, Lamp Control, PC & Printer, Password, and Module Options.  
Instrument Setup  
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Standard Operations  
5.2 Instrument Setup mode  
1. Select Instrument Setup in the Main Menu.  
A selection of functions appears in order to configure the functions  
of the instrument.  
This display appears when the multi-cell holder is installed or the  
13 mm round cuvette/vial compartment is used. If the imstrument  
recognizes a Sipper module or a Peltier module the Carousel  
Options key is replaced by Sipper Options or Peltier Options.  
Optional modules for the DR5000:  
•
•
•
•
Carousel Holder (Sample Changer)  
Pour-Thru Cell module  
Sipper module  
Peltier module (Temperature control module)  
Refer to the corresponding manuals/instruction sheets for more  
information.  
5.2.1 Operator ID  
Use this option to enter up to 30 sets of operator initials (up to  
5 characters each) into the instrument. This feature helps record  
which operator measured each sample.  
1. Press Operator ID in the Instrument Setup.  
2. Press New to enter a new Operator ID.  
3. Use the alphanumeric keypad to enter a new Operator ID.  
4. Press OK to confirm.  
5. The display shows the chosen Operator ID.  
6. Press OK. The instrument will return to the Instrument Setup  
screen and show the selected operator identifier.  
7. The chosen Operator ID is activated.  
Note: Press Delete to remove an Operator ID from the list.  
Note: Alternatively, enter or change an Operator ID in measurement mode.  
In the results screen, press Options>More>Instrument Setup or if an  
Operator ID is already assigned, select the "Operator ID symbol  
immediately in the results screen.  
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Standard Operations  
5.2.2 Sample ID  
Use this option to enter up to 30 Sample Identification tags (up to  
13 characters each) into the instrument. Sample IDs can be used to  
specify the sample location or other sample specific information.  
1. Press Sample ID in the Instrument Setup.  
2. Press New to enter a new Sample ID.  
3. Use the alphanumeric keypad to enter a new Sample ID.  
Note: If a USB Barcode handset scanner ( see Section 9 on page 129)  
is connected, Sample IDs can also be scanned.  
4. Press OK to confirm.  
5. To number the Sample IDs sequentially (e.g. Inflow (01 etc.)),  
select Add Number.  
•
•
Use the arrow keys to specify the first number of the  
sequence.  
Use the key between the arrow keys to enter the first  
number of the sequence using the alphanumeric keypad.  
6. Press OK to return to "Instrument Setup".  
7. The Sample ID is activated. Each Sample ID is automatically  
numbered in ascending order after a measurement. The  
number is shown in parentheses behind the Sample ID.  
To remove a Sample ID, highlight the ID and press Delete.  
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Standard Operations  
There are two ways to enter or change a Sample ID in the  
Measurement Mode:  
Option 1  
In the result display, press Options>More>Instrument Setup. If a  
Sample ID is already assigned, select the Sample ID symbol in the  
result display.  
Option 2  
With an USB keyboard (with US keyboard layout) or a scanner a  
Sample ID can be directly entered in the result display.  
5.2.3 Date and time  
1. Press Date & Time in the Instrument Setup.  
2. The date and time are subdivided over a number of fields.  
Press the appropriate field and use the arrow keys to change  
the value.  
3. Press OK to confirm. The instrument will return to  
Instrument Setup.  
5.2.4 Display and sound preferences  
1. Press Display & Sound in the Instrument Setup.  
The following options will be displayed:  
•
Display Contrast—Adjusts the display brightness to suit  
lighting conditions.  
•
•
Screen touch—Activates//Deactivates a short beep each time  
the screen is pressed (Default:off).  
Reading done—Activates/Deactivates a sound when a  
reading is complete (Default: short beep every time a reading is  
complete).  
•
Timer—Adjusts the length of the timer sound. Select Short or  
Long. Long beeps are recommended for noisy environments.  
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Standard Operations  
2. Select Long to change the number of audio signals.  
Use the alphanumeric keypad to enter/specify the number of  
audio signals (4–25).  
Note: A high number of audio signals increases the duration of the  
tones and a small number of audio signals reduces the duration of the  
tones.  
3. Press OK to confirm. The selected number of the audio signals  
sounds as a corresponding acoustic signal.  
4. Press OK to confirm. The instrument will return to  
Instrument Setup.  
5.2.5 Lamp Control  
The tungsten lamp produces light in the visible spectrum 320 to  
1100 nm.  
The deuterium lamp (UV-lamp) available produces light in the  
ultraviolet spectrum 190 to 360 nm.  
In the overlap zone from 320 to 360 nm, either the deuterium lamp  
(UV-lamp) or the tungsten lamp can be used for measurements.  
The lifetime of the lamps is influenced  
•
•
by on-off operation and the length of time in use.  
Typical use is to turn the instrument on for the entire 8–10 hour  
shift, and then off until the next day.  
Note: In general, avoid on/off cycles of the lamp; this shortens the lamp’s  
life span. For maximum life, turn the lamp off only if it will remain off for at  
least 4–5 hours.  
The lamp switches on automatically  
•
•
if a lamp is needed for the selected program  
if the instrument is operating inside the lamp’s spectrum  
5.2.5.1 VIS-Lamp and UV-Lamp  
1. Press Lamp Control in the Instrument Setup.  
2. Select On to switch on the VIS-Lamp and/or UV-Lamp.  
3. Select Save: in order to define a time interval for the burning  
time of the VIS lamp and/or UV lamp.  
4. Press the field below Save to select the lamp burning time.  
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Standard Operations  
5. Select the length of time the lamp will be switched on.  
Note: After this period of time the lamp automatically turns off, after no  
measurement has been made outside the corresponding range (UV/VIS).  
6. Touch OK to confirm.  
7. Touch UV Switch key to select the wavelength value between  
320 nm and 360 nm, at which the instrument changes from the  
visible to UV source.  
8. Use the alphanumeric keypad to enter the maximum  
wavelength for UV operation.  
9. Touch OK to confirm and return to “Lamp Control“.  
10. Touch OK to confirm and return to “Instrument Setup“.  
Note: The selected program that requires a lamp has highest priority. If the  
lamp in the Lamp Control was turned off, the lamp will be turned on  
automatically if it is needed for the stored program.  
5.2.5.2 Effect caused by the lamp settings on the Measuring Mode  
UV-Lamp switches on manually. The icon ”UV Lamp“ appears in the  
display and flashes.  
The instrument will display ”Lamp Warmup...“. This takes about  
3 minutes.  
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Standard Operations  
When the lamp is warmed up and ready, the UV icon stops flashing.  
Note: If both lamps are On, the icon UV-VIS is displayed in the selected  
Measurement Mode.  
Note: Alternatively you can touch the UV- or VIS-Lamp icon on the  
measurement screen to change the actual setting.  
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Standard Operations  
5.2.6 PC and printer  
The instrument is provided with 3 USB interfaces, which are located  
on the back of the instrument (see Figure 1 on page 14). These  
interfaces can be used for exporting data and graphics to a printer,  
updating data and for data communication to a personal computer.  
These interfaces can be used for the connection of a USB stick, an  
external USB keyboard or a USB Barcode handset scanner.  
Note: A USB hub may be used to connect several accessories at a time.  
A USB memory stick is used to upgrade data and software, see  
section 6.8.2 on page 106.  
Important Note: A screened USB cable must not be longer than  
3 m!  
Table 3 USB connector  
USB Interfaces  
USB (Type B)  
Description  
This USB interface is only intended to connect the instrument to a PC (with installation of the  
HACH Data Trans Software).  
These USB ports can be used to connect a printer, a USB memory stick, a barcode scanner or a  
keyboard.  
USB (Type A)  
1. Press PC & Printer in the Instrument Setup.  
A list with information about the connections opens.  
5.2.6.1 Printer setup  
For reasons of compatibility, the printer language must be  
HP PCL 3.  
2. Press Printer.  
3. Press Setup to display the Printer Setup screen.  
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Standard Operations  
Printer Setup:  
•
•
Resolution: Print quality  
Paper: Paper size  
Note: If an optional Thermal Printer is connected, the function "Auto Send"  
on/off is available.  
4. Select Auto-Send: On to send all measured data automatically  
to the Thermal printer.  
Note: The option Auto-Send is not available for any other printer (e.g. ink  
jet printer).  
5. Press Resolution to select the print quality.  
Select between  
•
•
•
100 dpi  
150 dpi and  
300 dpi  
6. Press OK to confirm.  
Note: Press OK again to return to the PC & Printer menu.  
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Standard Operations  
7. Press Paper to select the paper size.  
Select between  
•
•
•
•
Letter  
Legal  
Executive  
A4  
8. Press OK to confirm.  
Note: Press OK again to return to the PC & Printer menu.  
5.2.6.2 Print data  
1. Press Recall Data in the Main Menu.  
2. Select the data source, where the data to be printed are stored.  
3. A list is displayed. Data can be filtered. For more information  
see section 5.3.1.2 on page 35.  
4. Press the PC & Printer icon to send the data (table, curve)  
immediately to the printer.  
5. Highlight Single point or Filtered data or All data and press  
OK to confirm.  
Sending Data... is displayed until the data have been printed.  
5.2.6.3 HACH Data Trans  
The optional HACH Data Trans software must be installed on the  
PC for the subsequent to process for measurement data.  
1. Press PC & Printer in the Instrument Setup.  
2. Select PC.  
3. Press Setup to display the PC Setup screen.  
For further installation instructions, refer to the HACH Data  
Trans user manual.  
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Standard Operations  
4. Select Auto-Send: On to send all measured data automatically  
to the PC.  
Note: If Auto-Send: Off is selected, the PC & Printer icon must be  
pressed, in order to send data to the PC.  
Note: The remote function is only for monitoring the data transfer.  
5.2.7 Password  
The Password menu contains a variety of security settings to  
control access to various functions. For example, prevent  
unauthorized changes to stored programs or instrument  
configurations.  
1. Press Password in the Instrument Setup menu.  
2. In order to highlight the Security List assign a password. Press  
Set Password.  
3. Use the alphanumeric keypad to enter a new Password (up to  
10 characters each) and press OK to confirm.  
The access to the Security List is activated.  
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Standard Operations  
4. Press Security List to lock various functions for unauthorized  
users.  
5. Highlight the desired functions to control.  
6. Confirm the Security List with OK to return to the Password  
menu.  
7. Press On to highlight the new settings of the Security List.  
8. Enter the new Password again to confirm.  
9. Press OK to return to Instrument Setup.  
Note: The alphanumeric keypad to the Password inquiry appears when a  
user tries to reach a locked setting.  
5.2.7.1 Password deactivation  
1. Press Password in the Instrument Setup.  
2. Use the alphanumeric keypad to enter the former Password  
and press OK to confirm.  
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Standard Operations  
3. Press Off to deactivate the settings of the Security List.  
4. Press OK to return to Instrument Setup.  
Note: Use this function to delete the former Password or to enter a new  
one.  
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Standard Operations  
5.3 Store, recall, send and delete data  
5.3.1 The Data Log  
The Data Log will store up to 1000 readings taken in the modes:  
Stored Programs, User Programs, Favorite Programs, Single  
Wavelength and Multi Wavelength. A complete record of the  
analysis is stored, including the Date, Time, Results, Sample ID,  
and Operator ID.  
5.3.1.1 Auto/manual data storage  
The data storage parameter indicates whether data are to be stored  
automatically or manually (in which case the user has to decide  
which data to store).  
1. Press Store: On/Off in the Options menu.  
•
•
With the Store On setting, all measurement data are stored  
automatically.  
With the Store Off setting, no measurement data are  
stored. However, this setting can be changed to Store On  
in the result display through configuration. The reading  
currently shown in the display is then stored.  
Note: When the instrument's memory (data log) is full, the oldest data are  
automatically deleted allowing the new data to be stored.  
5.3.1.2 Recall stored data from the data log  
1. Press Recall Data in the Main Menu.  
2. Press Data Log.  
A listing of the stored data is displayed.  
3. Press Filter: On/Off.  
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Standard Operations  
4. The function Filter Settings is used to search for specific  
items.  
5. Highlight On to turn on the filters to select data by  
•
•
•
•
Sample ID  
Operator ID  
Start Date  
Parameter  
or any combination of the four.  
6. Press OK to confirm the selection. The chosen items are listed.  
7. Press View Details to get more information.  
5.3.1.3 Send data from the data log  
Data is sent from the data log as CSV (Comma Separated Value)  
files through a USB memory stick to a file named DATALOG. The  
file can then be processed using a spreadsheet program. The file  
name will be formatted as:  
DLYear_Month_Day_Hour_Minute_Second. CSV.  
To send data to a Printer, see section 5.2.6.2 on page 31.  
1. Plug in the USB device (Figure 1 on page 14).  
2. Press Recall Data from the Main Menu. Press Options and  
then the PC & Printer icon  
3. Select the data to send to the memory stick and press OK.  
Note: The number in parenthesis is the total number of data sets  
assigned to this selection.  
To send measurement data to a PC:  
The optional HACH Data Trans software must be installed on the  
PC, see section 5.2.6.3 on page 31.  
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Standard Operations  
5.3.1.4 Delete stored data from the data log  
1. Press Recall Data in the Main Menu.  
2. Press Data Log>Options>Delete.  
3. Highlight Single Point or Filtered data or All data and press  
OK to confirm.  
Note: The number in parentheses is the total number of data sets  
assigned to this selection.  
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Standard Operations  
5.3.2 Store, recall, send and delete data from wavelength scan and time course  
The instrument can store 20 Wavelength Scans and 20 Time  
Course Data sets.The data can be stored manually at the user's  
discretion after viewing the data.  
5.3.2.1 Data storage from wavelength scan or time course  
1. Press the Store icon in the Options menu after a reading is  
taken.  
The Store Data list will be displayed.  
2. Press Store to save the current scan to the highlighted  
numbered line. A scan can also be overwritten.  
5.3.2.2 Recall stored data from wavelength scan or time course  
1. Press Recall Data in the Main Menu.  
a. Select Wavelength Scan or Time Course to recall data.  
b. If a program is already in progress, press  
Options>More>Recall Data.  
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Standard Operations  
2. Press Graph to look at details.  
Note: Press View Summary to return to the Recall Data list.  
3. Press Table to look at details.  
Note: Press View Summary to return to the Recall Data list.  
5.3.2.3 Send data from wavelength scan or time course  
There are two ways to recall sent data to a USB memory stick,  
printer or PC with Hach Data Trans.  
Option 1:  
1. Press Recall Data in the Main Menu and then Wavelength  
Scan or Time Course.  
2. Press Options and then the PC & Printer icon to send the  
data to a USB memory stick, to a printer or to a PC with Hach  
Data Trans.  
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Standard Operations  
•
•
When a printer is connected, select how to send the data to  
the printer (graph, table or both graph and table).  
When a USB memory stick is connected, the files will be  
automatically sent as CSV files (Comma Separated Value)  
to a file ”WLData“ (Wavelength Scan Data) or ”TCData“  
(Time Course Data) to the USB memory stick.  
The file name will be formatted as: ”ScanData_X.csv“  
(Wavelength Scan Data) or ”TCData_X.csv” (Time Course  
Data).  
X = number of scans (1–20)  
For further processing use a spreadsheet program.  
Note: The advice ”Data already exist. Overwrite?“ appears when the files  
were already stored. Press OK to overwrite the stored data.  
Option 2:  
1. Press Wavelength Scan or Time Course and then  
Options>More>Send Data to send the data to a USB memory  
stick or to a printer.  
•
•
When a printer is connected, select how to send the data to  
the printer (graph, table or both graph and table).  
When a USB memory stick is connected, the files will be  
automatically sent as CSV files (Comma Separated Value)  
to a file ”WLData“ (Wavelength Scan Data) or ”TCData“  
(Time Course Data).  
The file name will be formatted as:  
”ScanData_Year_Month_Day_Hour_Minute_Second.CSV“  
(Wavelength Scan Data) or  
The file name will be formatted as:  
”TCYear_Month_Day_Hour_Minute_Second.CSV“ (Time  
Course Data).  
For further processing use a spreadsheet program.  
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Standard Operations  
5.3.2.4 Delete stored data from wavelength scan or time course  
1. Press Recall Data from the Main Menu and then Wavelength  
Scan or Time Course or Options>More>Recall Data.  
A listing of the stored data is displayed.  
2. Highlight any data to delete.  
3. Press Delete in the Options menu and press OK to confirm.  
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Standard Operations  
5.4 Stored Programs  
The instrument contains more than 200 programmed procedures.  
They can be accessed through the Stored Programs menu.  
5.4.1 Select a saved test/method; enter user-specific basic data  
4. Press Stored Programs in the Main Menu to view an  
alphabetical list of stored programs with program numbers.  
The Stored Programs list will appear.  
5. Highlight the required test.  
Note: Select the program number by name or use the arrow keys to  
scroll through the list quickly and highlight the program or press  
Select by number to search for a specific program number. Use the  
alphanumeric keypad to enter the test number and press OK.  
6. Press Start to run the program. After a program is selected, the  
screen for that parameter will appear.  
Note: All corresponding data (wavelength, factors and constants) are  
already preset.  
7. Follow the chemical procedures described in the corresponding  
Procedures Manual.  
5.4.2 Stored program options  
1. From the Main Menu, select Stored Programs. Select the  
necessary method and press Start.  
2. Press Options for Parameter Setup. Refer to Table 4 for stored  
program descriptions.  
Table 4 Stored programs options  
Options  
More  
Description  
For further Options  
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Standard Operations  
Table 4 Stored programs options (continued)  
Description  
Options  
With the Store On setting, all measurement data are stored automatically. With the Store Off  
setting, no measurement data are stored.  
Store Off/On  
% Trans/Conc/Abs  
To switch to % transmittance, concentration or absorbance readings  
Send Data icon /  
Send Data  
To send Data to a printer, computer or USB memory stick (Type A)  
This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed  
(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has  
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement  
program.  
Timer icon  
Single Reading Mode: A reading is only displayed after a measurement has been carried out  
(press Read; standard setting) (see section 5.4.4.1 on page 45).  
Reading Mode  
Continuous Reading Mode: After the zero measurement, all readings are displayed  
automatically and continuously (see section 5.4.4.2 on page 45).  
A corrective dilution factor can be entered in order to take account of certain properties.  
The number entered at the dilution factor prompt will be multiplied by the result to compensate for  
the adjustment. For example, if the sample has been diluted by a factor of 2, enter 2. The default  
setting of the dilution factor is turned off.  
Dilution Factor Off/On  
Standard Addition  
Note: When a dilution is in effect, the dilution icon will appear on the display.  
This enables the accuracy of the measurements to be checked. The (working) procedure for a  
test parameter contains a detailed explanation of how to use this function.  
The (working) procedure for a test parameter indicates whether a standard adjustment is  
necessary and, if so, how to proceed.  
Standard Adjust  
Chemical Form  
Some of the stored tests/methods allow to select the chemical form and the measuring range.  
Some of the stored tests/methods include the "Reagent Blank" function. This enables the reagent  
blank value to be added to or subtracted from, the subsequent readings. The reagent blank value  
shifts the calibration curve along the y-axis, without changing the shape or gradient of the curve.  
The effect corresponds to a y-axis intercept of the calibration straight line. This is made clear by  
the following equation: Concentration = [(Conc. factor) * Abs] – (reagent blank value).  
Reagent Blank  
Save as User Program  
Recall Data  
To store the selected parameters as a User Program, see section 6.1 on page 61.  
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on  
page 35.  
Instrument Setup  
Basic data of the instrument, see section 5.2 on page 23.  
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Standard Operations  
5.4.3 Use of program timers  
Some procedures do not require the use of timers. Other  
procedures require several timers. These timers are  
pre-programmed into each Stored Program, along with a  
description of the activity to be performed during the timed period.  
1. Press the Timer icon on the display.  
2. Press OK to start the first timer.  
The timer will count down on the screen.  
3. To start the next timed activity for the Stored Program, press  
the Timer icon and OK.  
Note: Press Close to view the measurement screen while the timer is  
running. The time will be shown left side bottom instead of the date.  
Note: Press Cancel to stop the timer midway through the countdown.  
Note: The Timer will beep when the end of the running time is reached.  
A general purpose timer is also available in many programs. When  
the timer icon is visible, press the icon and select General Timer.  
A new screen will appear. Enter the length of the timed interval and  
press OK to start the timer. The timer will beep when the timed  
interval ends.  
5.4.4 Set the reading mode  
1. To highlight the required mode, press Reading Mode.  
2. Select the required mode, then press OK, then Return to return  
to the result display.  
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5.4.4.1 Take single wavelength measurements (single reading)  
1. Insert the blank cuvette into the cuvette/sample cell holder.  
Press Zero.  
Note: The Read key is only active after the zero measurement has  
been carried out.  
2. Insert the sample cuvette into the cuvette/sample cell holder.  
Press Read.  
Note: For data storage, see section 5.3.2 on page 38.  
5.4.4.2 Take single wavelength measurements (continuous readings)  
1. Insert the blank cuvette into the cuvette/sample cell holder.  
Press Zero.  
Note: In the reading mode ”Continuous“ only the Zero key is shown to  
start the reading. The reading sequence is started automatically.  
2. Insert the sample cuvette into the cuvette/sample cell holder.  
3. Press Options and then the Store icon to store the displayed  
data in the Data Log.  
Note: For data storage, see section 5.3.2 on page 38.  
5.4.5 Set the dilution factor  
The Dilution Factor function is used to calculate the original  
concentration of a sample that has been diluted by a known ratio.  
For example, dilution factor can bring the analyzed concentration  
within the test range.  
1. Press Options>More…>Dilution Factor.  
The number entered at the dilution factor prompt will be  
multiplied by the result to compensate for the adjustment.  
For example, if the sample has been diluted by a factor of 2,  
enter 2. The default setting of the dilution factor is turned off.  
2. Press OK to confirm.  
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Standard Operations  
3. Press Return to return to the result display.  
4. Confirm ’Store present reading again?’ to save the current  
measured value with the dilution factor.  
Note: When a dilution is in effect, the dilution icon will appear on the  
display.  
Note: If using undiluted samples, set the dilution factor off again.  
5.4.6 Run a standard adjust  
The Standard Adjust function allows the calibration curve for a  
stored program to be adjusted based on analysis of a known  
standard solution. The Accuracy Check section of written  
procedures often suggests a standard solution concentration for  
this purpose.  
Read a standard before setting Standard Adjust to On.  
1. Follow the entire procedure, using a known standard for the  
sample.  
2. After reading the concentration, press  
Options>More>Standard Adjust.  
3. If Standard Adjust is set to Off, turn it On.  
The Current Reading will show the concentration. The box on  
the right will show the default standard value for the test, as  
mentioned in the procedure.  
4. If the measurement used a standard concentration that is  
different from the one displayed in the box, press the box on the  
right to enter a different standard value and enter the new  
value. Press OK to confirm.  
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Standard Operations  
5. Press Adjust to enable the Standard Adjust. The Standard  
Adjust icon will appear.  
Note: The adjustment must be within certain limits, which vary with  
each program. The allowable percentage is shown after "Adjustment“.  
Note: When a Standard Adjust is in effect, the Standard Adjust icon  
will appear on the display.  
5.4.7 Set the chemical form  
Some Stored Programs allow a variety of chemical forms to  
be selected.  
Press the unit (e.g. mg/L) or the chemical representation of the  
3+  
evaluation form (e.g. Al ). A list of available evaluation forms is  
displayed. Select the required form by pressing the corresponding  
entry in the list.  
Note: When the program is exited, the evaluation form reverts to the  
standard setting.  
An alternative way of changing the standard setting:  
1. Press Options>More>Chemical Form.  
2. Select the Chemical Form.  
Note: The stoichiometric conversion of the measurement result is  
carried out automatically.  
Note: The selected Chemical Form will appear on the display. Test  
results will be calculated and displayed in this chemical form.  
5.4.7.1 Change of the default setting of the chemical form  
1. Insert the sample cuvette or blank (depending on the working  
procedure) into the cell compartment.  
2. In the result display, press Options>More>Chemical Form.  
3. A list of available evaluation forms appears. Select the new  
default setting.  
4. Press Save as Default.  
The current result and all further measurements will be displayed in  
the new chemical form.  
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Standard Operations  
5.4.8 Run a reagent blank  
Some of the stored tests/methods include the "Reagent Blank"  
function. This enables the reagent blank value to be measured and  
then taken into account in calculating the measurement result.  
Measurement/analysis of a reagent blank:  
1. Prepare the test/method in accordance with the (working)  
procedure. Instead of a sample, deionized water is used to  
determine the reagent blank value.  
2. Select the test. If required by the (working) procedure, insert  
the cell with deionized water into the cell compartment. Press  
Zero.  
3. Insert the prepared sample cell into the cell compartment.  
Press Read. The result is displayed.  
4. Press Options>More>Reagent Blank.  
5. Press On to highlight the Reagent Blank function.  
6. The concentration shown on the key is the measured value of  
the reagent blank. To use this value for more analyses of this  
parameter, press OK.  
To enter a previously recorded reagent blank value, press the key  
and use the alphanumeric keypad.  
7. Press OK.  
Note: The Reagent Blank function is deactivated when the measurement  
program is left. To use the same blank value later for other tests using the  
same reagent lot, enter the value per step .  
Note: The results calculated using the reagent blank value must lie within  
the limits of the measuring range of the test/method.  
Note: The reagent blank icon is shown in the result display (see arrow)  
when the function is active.  
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Standard Operations  
5.4.9 Analysis of samples  
1. Press Stored Programs and select a program.  
2. Insert the blank cuvette/cell into the cuvette/sample cell holder.  
3. Press Zero.  
4. Remove blank cuvette/cell and insert sample cuvette/cell into  
the cell compartment.  
5. Press Read. The result will be displayed.  
6. For data storage, see section 5.3.2 on page 38.  
5.4.10 Add stored programs to the favorite programs list  
The Favorites menu simplifies test selection by creating a list of the  
most frequently used tests from the Stored Programs and  
User Programs.  
1. Press Stored Programs in the Main Menu. The Stored  
Programs list will appear.  
2. Highlight the selection by pressing it or Select by Number to  
search for the program by number.  
3. Press Add to Favorites and press OK to confirm.  
The program can now be selected from Favorite Programs  
menu in the Main Menu.  
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Standard Operations  
5.5 Barcode Programs  
A special barcode reader in cell compartment #1 automatically  
reads the barcode on the 13 mm cuvette/vial as the cuvette/vial  
completes a single rotation. The instrument uses the barcode  
identification to automatically set the correct wavelength for the  
analysis and calculates the result immediately with the help of the  
stored factors.  
In addition, measured values are recorded at 10 different positions  
during a rotation. A special outlier-elimination program is run and  
then the average of the measured values is calculated. Cuvette/vial  
errors and soiling are recognized and highly precise results are  
obtained.  
Figure 7 Cell compartment #1  
1
Cell compartment #1 for 13 mm barcode cuvettes/or 16 mm vials  
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Standard Operations  
5.5.1 Complete a barcode 13 mm test/vial  
Note: The cell compartment must be closed before a measurement is  
made!  
1. The instrument needs to be at the Main Menu.  
2. Prepare the barcode 13 mm cuvette/vial in accordance with the  
working procedure and insert the cuvette/vial in cell  
compartment #1 and close the cell compartment.  
When a coded cuvette/vial is placed in cell compartment #1  
(Figure 7), the corresponding measurement program is  
automatically activated in the Main Menu.  
The measurement is started automatically and the results are  
displayed.  
To evaluate other cuvette/vial tests and other parameters, insert the  
prepared cuvette/vial into the cell compartment and read the result.  
5.5.2 Select the measuring range  
Some tests can be used for different measuring ranges. After the  
sample cuvette/vial has been inserted, a list of the different  
measuring ranges is displayed.  
Select the required measuring range by highlighting the appropriate  
line.  
Press Start Permanent if this measuring range is to apply to all  
subsequent measurements.  
Changing the standard setting  
In the result display, press Options>More>Permanent: On. The  
key will change to Permanent: Off.  
5.5.3 Select the chemical evaluation form  
The chemical form of the test result of a number of parameters can  
be selected individually.  
In the result display, press the unit (e.g. mg/L) or the chemical  
3–  
representation of the evaluation form (e.g. PO –P). A list of  
4
possible evaluation forms is displayed, from which the required  
form can be selected. Press OK to confirm.  
Another way of changing the standard setting is:  
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Standard Operations  
1. In the result display, press Options>More>Chemical Form.  
A list of available evaluation forms appears.  
2. Select the required chemical form and press OK to confirm.  
Note: The selected chemical form is displayed, but does not become  
the default. To change the default, see section 5.5.3.1.  
5.5.3.1 Change of the default setting of the chemical form  
1. Insert the blank or sample cuvette/vial (depending on the  
working procedure) into the cell compartment and close the cell  
compartment.  
2. In the result display, press Options>More>Chemical Form.  
3. A list of available evaluation forms appear. Select the new  
default setting.  
4. Press Save as Default.  
The current result and all further measurements will be displayed in  
the new chemical form.  
5.5.4 Basic test-specific and sample-specific data settings  
Press Options to change test or sample specific settings.  
Table 5 Barcode program options  
Options  
More  
Description  
For further Options  
With the Store On setting, all measurement data are stored automatically. With the Store Off  
setting, no measurement data are stored.  
Store Off/On  
Abs % Trans  
To switch to % transmittance or absorbance readings  
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Standard Operations  
Table 5 Barcode program options (continued)  
Description  
Options  
Send Data icon /  
Send Data  
To send data to a printer, computer or USB memory stick (Type A)  
This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed  
(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has  
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement  
program.  
Timer icon  
A corrective dilution factor can be entered in order to take account of certain properties.  
The number entered at the dilution factor prompt will be multiplied by the result to compensate for  
the adjustment. For example, if the sample has been diluted by a factor of 2, enter 2. The default  
setting of the dilution factor is turned off.  
Dilution Factor Off/On  
Note: When a dilution is in effect, the dilution icon will appear on the display.  
Note: If undiluted samples are used, set the dilution factor off.  
Chemical Form  
Edit  
Some of the stored tests/methods allow to select the chemical form and the measuring range.  
To modify an existing program  
Save as User Program  
To store the selected parameters as a User Program, see section 6.1 on page 61.  
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on  
page 35.  
Recall Data  
Instrument Setup  
Basic data of the instrument, see section 5.2 on page 23.  
5.5.5 Sample blank  
Turbidity and color in the sample matrix can falsify the results of a  
photometric analysis. The interference factors come from the  
sample or are created by reactions with the reagents.  
The influence of turbidity and/or color can be eliminated or reduced  
by taking a sample blank reading.  
In the barcode mode, a special cuvette/vial (TNT919) containing  
the sample blank is placed in cell compartment #1 after the sample  
reading has been taken and is automatically measured. The  
sample reading is then corrected by adding or subtracting the blank  
value. The final result is displayed, with the message "After blank  
value corr.".  
Some barcode tests do not require a sample blank value to be  
determined, as turbidity and color are dealt with during the test  
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Standard Operations  
procedure. Other barcode tests use the reagent test vials to  
determine the sample blank. Please refer to the Hach procedure for  
method-specific instructions.  
5.5.6 Update/edit barcode tests  
5.5.6.1 Manual update of a barcode test  
Using the data provided in the barcode, the instrument  
automatically sets the measurement wavelength and factors. If a  
discrepancy is detected between the barcode data and the stored  
data or a new test is identified, the instrument requests an update.  
Data types are described in Table 6.  
Table 6 Program update options  
Program point  
Name  
Description  
Name of the analysis parameter  
Version  
An abbreviation assigned by the user or the version number, etc. is entered here  
Exact definition of the test: the number of wavelengths at which measurements are carried out,  
the number of absorbance measurements needed, the keys to be used, any waiting periods  
between measurements, etc.  
Measurement process  
Formula  
Definition of the evaluation formulas with which the test result is calculated  
The number of variables shown in the display depends on the definition of the measurement  
Variables  
process and the formulas. Input of the numerical values of the wavelengths, factors, constants,  
etc.  
This function can be used to enter abbreviations and defined times for up to four timers. Highlight  
the appropriate line and press Edit.  
Timer 1, Timer 2,  
Timer 3, Timer 4  
The timers are activated or deactivated with the control boxes on the left of the display. In the  
next column, a selection can be made from a list of names that describe the corresponding work  
step. In the third column, the times for each active timer are entered.  
Enter the Name  
Check the working procedure in advance to determine whether this  
point needs to be changed.  
1. Highlight the line containing Name and press Edit. Use the  
alphanumeric keypad to enter the name indicated in the  
working procedure.  
2. Press OK to confirm.  
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Standard Operations  
Enter the Version  
1. Highlight the line containing Version and press Edit. Use the  
alphanumeric keypad to enter the name indicated in the  
working procedure.  
2. Press OK to confirm.  
Enter the Measurement Process  
For detailed information on entering the measurement process, see  
section 6.1.2 on page 72.  
Check the working procedure in advance to determine whether this  
point needs to be changed.  
1. Highlight the Measurement Process line in the data overview  
and press Edit.  
2. Press Edit, select the sequence to be edited and press Delete.  
3. Press New and use the alphanumeric keypad to enter the  
process indicated in the working procedure.  
Enter the Formula, Concentration Units, Designation,  
Measuring Ranges  
For detailed information on entering the Formula, see section 6.1.2  
on page 72.  
Check the working procedure in advance to determine whether this  
information needs to be changed.  
1. Highlight the Formula line in the data overview and press Edit.  
2. Select the formula, press Edit and use the alphanumeric  
keypad to enter the data specified in the working procedure (for  
C1=, C2=, units, name, measuring range limits, etc.).  
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Standard Operations  
Enter the Variables (Factors, Wavelength and Conversion  
Factors)  
For detailed information on entering the Variables, see section 6.1.2  
on page 72.  
Check the working procedure in advance to determine whether this  
point needs to be changed.  
1. Highlight the Variables line in the data overview and press  
Edit.  
2. Select the variable, press Edit and use the alphanumeric  
keypad to enter the data specified in the working procedure (for  
F1, F2, λ1, U1 etc.). Confirm each entry with OK.  
Abbreviation of Variables:  
F1: Factor 1  
F2: Factor 2  
λ1: Wavelength 1  
U1: Conversion Factor 1 for the first chemical form  
U2: Conversion Factor 2 for additional chemical forms etc.  
Timer 1, Timer 2, Timer 3 and Timer 4:  
The timers are activated or deactivated with the control boxes on  
the left of the display. In the next column, a selection can be made  
from a list of names that describe the corresponding work step. In  
the third column, the times for each active timer are entered.  
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Standard Operations  
5.5.6.2 Update an existing barcode test  
Note: Only in very few cases does the revision of a test require all test  
specifications to be updated.  
Option 1: Manual update of test data  
1. The instrument needs to be at the Main Menu.  
2. Insert the blank or sample cuvette/vial (depending on the  
working procedure) in cell compartment #1 and close the cell  
compartment.  
3. After "Barcode controlnumber?", a data overview is displayed,  
including the specifications of the test that is to be revised.  
Note: The working procedures of the corresponding test contains the  
new data (Wavelength, Factors, Measurement Ranges, Conversion  
Factor for chemical Form, etc.).  
4. Highlight the corresponding line and press Edit.  
5. When finished programming, press Store and then Cancel to  
return to the User Programs menu.  
6. Press Start to complete the test with the new data.  
.
Option 2: Manual check/revision of test data  
If an incorrect entry was made during the data update and is not  
recognized via the barcode of the cuvette/sample vial (e.g. an  
incorrect measuring range), another option is available to check  
and if necessary to update, the test data.  
1. The instrument need to be at the Main Menu.  
2. Insert the blank or sample cuvette/vial (depending on the  
working procedure) into the cell compartment #1 and close the  
cell compartment.  
3. Press Options>More>Edit.  
4. A data overview is displayed, including the specifications of the  
test that is to be revised. Compare the displayed data with the  
data in the working procedure and edit the displayed data in  
accordance with the working procedure.  
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Standard Operations  
5.5.6.3 Program a new test  
1. The instrument needs to be at the Main Menu.  
2. Insert the sample cuvette/vial or blank (depending on the  
working procedure) into the cell compartment #1 and close the  
cell compartment.  
3. After "Program not available", a data overview is displayed,  
including the specifications of the test that is to be revised.  
The working procedures of the corresponding test contains the  
new data (Wavelength, Factors, Measurement Ranges,  
Conversion Factor for chemical Form etc.).  
4. Highlight the corresponding line including the specifications of  
the test that is to be programmed and press Edit.  
5. To finish programming, press Store and then Cancel to return  
to the Main Menu.  
6. Press Start to run the test with the new data.  
5.5.7 Upgrade of the instrument software  
To obtain the software for the update from the Internet at  
www.hach.com:  
1. Go to http://www.hach.com.  
2. On the DR 5000 product page, click Lab System  
Software/Software Update Downloads under the heading  
Downloads.  
3. Locate the appropriate download and follow the prompts for  
saving the file(s) to the USB memory stick.  
4. On the DR 5000, press Instrument Update in the System  
Checks menu.  
5. Connect the USB stick to the USB interface on the DR 5000  
(section 3.3 on page 14). Press OK.The link is established  
automatically and the software is updated.  
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Standard Operations  
6. Press OK to return to the System Checks menu.  
Note: When the instrument software has been updated, a prompt to restart  
the instrument is displayed.  
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Standard Operations  
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Section 6 Advanced Operations  
6.1 User Programs  
User programs provide the opportunity to complete "made to  
measure" analysis.  
The User Programs database is empty when the instrument leaves  
the factory and is used to accommodate programs created by users  
specific needs. Here are a few examples of entries:  
•
Programming of user-created procedures. The analysis  
procedure must be developed first, before it can be  
programmed. The user must define or determine the program  
sequences, calculation formulas, measurement wavelengths,  
factors, measuring range limits, etc.  
•
•
Modified tests  
Assignment of user programs to the favorites menu for  
frequently used tests.  
•
Creation of a specific selection of methods and tests.  
Press User Programs in the Main Menu and then  
Program Options. The Program Options menu contains several  
input and editing options (Table 7):  
Table 7 Program options user programs  
Description  
Options  
1
New  
Select New to program a new user program.  
Note: The first time Program Options is selected, only the New option is available. The other  
options remain inactive (gray) until the first program has been created.  
1
1
1
Add to favorites  
Edit  
Select Add to favorites to add an existing user program to the list of frequently used programs.  
Select Edit to modify an existing program  
Delete  
Select Delete to remove a program from the list of user programs. The program will be  
simultaneously deleted from the Favorites list.  
6.1.1 Program a user method  
All input steps and their significance and options are explained in  
the following sections.  
1. Select New in the Program Options menu.  
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Advanced Operations  
Program Number:  
Specific test number, with which the program can subsequently be  
called up from the selection list in the User Programs menu or the  
Favorites menu.  
2. Use the alphanumeric keypad to enter a program number  
between 950 and 999. The lowest available number appears  
automatically.  
3. Press OK.  
Note: If the program number is already assigned to another user  
program, a message appears, asking whether the existing program  
should be replaced. Press OK to overwrite the existing program.  
Program Name:  
4. Use the alphanumeric keypad to enter a program name. The  
name can be a maximum of 28 characters long.  
5. Press Back to go back to the previous program point or press  
Next to continue with the input of the program data.  
Program Type:  
6. Select the required option (Table 8) and press Next.  
7. If the Single Wavelength (section 6.1.1.1 on page 63) or Multi  
Wavelength (section 6.1.1.2 on page 64) is selected, define the  
unit, wavelength, absorbance formula, wavelength λx,  
concentration factor Kx, concentration resolution, chemical  
form and calibration equation.  
More information on Free Programming parameters see  
section 6.1.2 on page 72.  
Table 8 Program descriptions  
Program Type  
Description  
Measurements at a defined wavelength  
Single Wavelength  
In the Multi Wavelength mode, absorbance values can be measured at up to four wavelengths  
and the results can be mathematically processed to obtain sums, differences and relationships.  
Multi Wavelength  
Free Programming  
This is an advanced form of programming for original user-developed methods. In Free  
Programming, the user defines the measurement process, variables and calculations involved in  
obtaining a reading.  
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Advanced Operations  
6.1.1.1 Single wavelength settings  
If the Single Wavelength mode is selected, the following  
parameters can be defined:  
Units:  
Select the required unit from the list and press Next.  
Note: Units of measure not included in this list can be added in the edit  
program under Program Options, Edit. Select Units, Edit and then New.  
Wavelength (single wavelength program type):  
Use the alphanumeric keypad to enter the measurement  
wavelength. The entered wavelength must be in the range from  
190–1100 nm.  
Press Next to proceed.  
Concentration resolution (number of decimal places)  
Select the required number of decimal places from the displayed  
list and press Next.  
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Advanced Operations  
Chemical form:  
Enter the chemical formula used in the display to represent the  
analysis parameter.  
Use the alphanumeric keypad to enter the chemical form and press  
Next to enter Calibration settings.  
6.1.1.2 Multi wavelength settings  
If the Multi Wavelength mode is selected, the following parameters  
can be defined:  
Units:  
Select the required unit from the list and press Next.  
Note: Units of measure not included in this list can be added in the edit  
program under Program Options, Edit. Select Units, Edit and then New.  
Absorbance formula (multi-wavelength program type):  
The Absorbance Formula menu is used to define the wavelengths  
and the coefficients used in the formula. The absorbance formula  
defines the calculation for the multi-wavelength measurement.  
Press the appropriate key to edit the input.  
Press the Formula key.  
In the displayed list, select the formula for the program and press  
OK to confirm.  
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Advanced Operations  
List of available absorbance formulas  
A is the absorbance at wavelength 1,  
1
A is the absorbance at wavelength 2 and so on  
2
K is the factor at wavelength 1,  
1
K is the factor at wavelength 2 and so on  
2
If a subtraction has to be completed, the factors can be entered  
with a minus sign.  
Wavelength λ :  
x
Press a λ key and use the alphanumeric keypad to enter a  
x
wavelength. Press another λ key and enter the next wavelength. If  
x
necessary, repeat until all the wavelengths for the formula have  
been entered. The wavelengths must be in the range from  
190–1100 nm. Press OK.  
Concentration Factor Kx  
Multiplication factor for converting absorbance values into  
concentration values.  
Press a factor key and use the alphanumeric keypad to enter a  
factor. If the formula includes more than one factor, press another  
factor key and enter another factor and repeat until all the factors  
have been entered. Press OK. When all the relevant data have  
been entered, press Next.  
Note: Up to five digits can be entered, including a maximum of 4 decimal  
places to the right of the decimal.  
Concentration resolution (number of decimal places)  
Select the required number of decimal places from the displayed  
list and press Next.  
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Advanced Operations  
Chemical form:  
Enter the chemical formula used in the display to represent the  
analysis parameter.  
Use the alphanumeric keypad to enter the chemical form and press  
Next to enter Calibration settings.  
6.1.1.3 Calibration settings for single and multi wavelength mode  
A method is calibrated by determining the absorbance values of  
several standard solutions of known concentration.  
There are three ways to create and store a calibration curve.  
Instructions for each method follows (Table 9).  
Table 9 Calibration settings  
Mode  
Descriptions  
A calibration table is created by entering the concentration values and the absorbance values of  
the analyte solution. The absorbance values are plotted versus standard concentrations and the  
calibration curve is displayed as a graph (page 66).  
Enter values  
A calibration table is created by entering the concentration values of the standard solutions and  
then measuring the absorbance of the analyte solutions. The absorbance values are plotted  
versus standard concentrations and the calibration curve is displayed as a graph (page 68).  
Read Standards  
Enter Formula  
If the calibration curve can be determined from the mathematical relationship between  
concentration and absorbance by linear regression, etc., the corresponding formula can be  
selected (linear, 2nd or 3rd order polynomial) from a list and the appropriate factors can be  
entered (page 69).  
Calibration by entering calibration values  
Enter concentration/absorbance  
1. Select the Enter Values and press Next.  
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Advanced Operations  
2. To enter the standard concentrations and corresponding  
absorbance values in the displayed table, press the "+" symbol.  
Use the alphanumeric keypad to enter the values.  
Press OK and enter the corresponding absorbance value.  
Press OK.  
The entered data are displayed in the table. Repeat the  
sequence for each data point to enter it.  
3. To change a value in the table, highlight the appropriate line,  
press the unit key (e.g. mg/L) or Abs and enter the changed  
value via the alphanumeric keypad.  
4. When the data have all been entered, press Graph.  
2
Note: The correlation coefficient (r ) is shown on the left below the  
axes.  
5. The linear equation corresponds to the standard setting. Press  
Next Curve to display the polynomial 2nd order curve. Press  
Next Curve again, to display the polynomial 3rd order curve.  
6. Press Force 0 to change the setting from Off to On. The curve  
now passes through the origin of the coordinate system.  
Note: This may have an adverse effect on the correlation coefficient  
2
(r ).  
7. Press Table to display the table again.  
8. When the table has been completed and the curve type has  
been chosen, press Done when the graph is displayed or Exit  
when the table is displayed. Go to section 6.1.1.4 on page 70.  
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Advanced Operations  
Calibration by reading standards  
1. Press Read Standards and press Next.  
2. To enter the standard concentrations in the displayed table,  
press the "+" symbol. Use the alphanumeric keypad to enter  
the standard concentration. Press OK.  
3. Press the "+" symbol again (see arrow) and enter the next  
standard concentration. Repeat this sequence until all standard  
conentrations (maximum of 24 solutions) have been entered.  
4. Highlight the line with the appropriate concentration and insert  
the cuvette/cell with the corresponding standard solution.  
5. Insert the zero solution into the cell compartment. Press Zero.  
6. Insert the first standard solution into the cell compartment.  
Press Read.  
Insert the second standard solution into the cell compartment.  
Press Read.  
Repeat this sequence until all the standard solutions have been  
measured (maximum of 24 solutions).  
The entered and measured data are displayed in the table.  
Note: To delete a standard concentration, highlight the appropriate line and  
press the Delete icon.  
The timer icon shown in the display helps to ensure, when  
necessary, that the steps of an analysis are correctly timed (e.g.  
reaction times, wait times, etc., can be exactly specified). When the  
specified time has elapsed, an acoustic signal is emitted. The use  
of the timer has no influence on the measurement program.  
7. When the data have all been entered and the measurements  
have all been completed, press Graph.  
8. The linear curve corresponds to the standard setting. Press  
Next Curve to display the polynomial 2nd order curve.Press  
Next Curve again to display the polynomial 3rd order curve.  
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Advanced Operations  
9. Press Force 0 to change the setting from Off to On. The curve  
then passes through the origin of the coordinate system.  
Note: This may have an adverse effect on the correlation coefficient  
2
(r ).  
10. Press Table to display the table again.  
11. When the table has been completed and the curve type has  
been chosen, press Done when the graph is displayed or Exit  
when the table is displayed. Go to section 6.1.1.4 on page 70.  
Calibration by entering the formula  
1. Press Enter Formula and press Next.  
2. Press the formula key.  
A list of available formulas (linear and 2nd and 3rd order  
polynomial) is displayed. Up to 4 coefficients can be entered,  
depending on the selected formula. Press the required formula.  
3. Depending on the selected formula, the required coefficients  
(a, b, c...) are displayed. Press the coefficient keys and enter  
the corresponding values via the alphanumeric keypad. After  
each entry, press OK to confirm.  
Note: The coefficients can have 5 digits and can have a positive or a  
negative sign.  
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Advanced Operations  
6.1.1.4 Store a user program  
The input of the basic data is complete. An overview of the variable  
program data is displayed.  
4. To enter more specifications or change existing ones, highlight  
the appropriate line and press Edit.  
5. Select Store to save the user program.  
6. Press the PC & Printer icon to send the program data to a  
printer or to a USB memory stick (connect the USB memory  
stick to the USB interface first).  
Note: The test data will be formatted in the preinstalled PrgData folder  
as a .csv and as a .lst file.  
7. To transfer the program data from one instrument to another  
create a new folder on the USB memory stick named dbhl and  
copy the .lst files into this folder. (see section 6.8.2 on  
page 106).  
6.1.1.5 Additional user-defined parameters and functions  
In addition to the previously defined basic data, additional  
parameters and functions can be defined for user programs:  
•
•
•
measuring range upper and lower limits  
timer functions  
chemical forms  
Upper and lower limits of the measuring range  
It is possible to enter a maximum (upper) and minimum (lower)  
measurement value. An error message is displayed if a reading is  
above the upper limit or below the lower limit.  
1. Highlight the appropriate line in the overview of the program  
data and press Edit.  
2. Set On and press the 0.000 key to enter the measuring range  
limit. Confirm the entry by pressing OK.  
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Advanced Operations  
Timer 1 / Timer 2 / Timer 3 / Timer 4:  
This function can define time intervals for up to four timers. Timer  
designations such as Shake, Wait and Swirl can be assigned.  
1. Highlight the appropriate line in the overview of the program  
data and press Edit.  
2. The timers are activated or deactivated with the check boxes in  
the left part of the display. In the next column, a selection can  
be made from a list of names that designate the corresponding  
work step. In the third column, the times for each timer are  
entered (in mm:ss).  
Chemical form 2 / chemical form 3 / chemical form 4:  
If a Chemical Form 1 has been defined, up to three additional  
alternative forms can be entered here.  
1. Highlight the appropriate line in the overview of the program  
data and press Edit.  
2. The chemical forms are activated or deactivated with the check  
boxes in the left part of the display.  
3. Press the left key to enter another chemical form with the  
alphanumeric keypad and press OK to confirm. Press the right  
key to enter the conversion factor to calculate the concentration  
of the additional chemical form from the concentration of  
Chemical Form 1 and press OK to confirm.  
4. Press Store to save the program data. Press Cancel to return  
to the Main Menu.  
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Advanced Operations  
6.1.2 Free programming program type  
Free Programming is an advanced option for entering original  
user-developed methods. When the Free Programming option is  
selected, an overview of the specifications of the programmed test  
is displayed. Each input option can be modified to develop the user  
method. Refer to Table 10 for more information. To modify an input  
option, select the appropriate line and press Edit.  
Important Note: Perform steps 1–6 of Programming a New User  
Method (section 6.1.1 on page 61) before proceeding with Free  
Programming.  
Highlight the line containing the program point that is to be edited or  
defined and press Edit.  
Table 10 Definitions of the program points  
Description  
Program point  
Name  
Name of the analysis parameter  
Version  
An abbreviation or version number assigned by the user is entered here.  
Exact definition of the test: the number of wavelengths at which measurements are made, the  
number of absorbance measurements needed, the keys to be used, any waiting periods between  
measurements, etc.  
Measurement Process  
Formula  
Definition of the formulas with which the test result is calculated.  
The number of variables shown in the display depends on the definition of the measurement  
Variables  
process and the formulas. Input of the numerical values of the wavelengths, factors, constants,  
etc.  
Used to enter abbreviations and defined times for up to four timers. Highlight the appropriate line  
and press Edit. The timers are activated or deactivated with the control boxes on the left of the  
display. In the next column, a selection can be made from a list of names that describe the  
corresponding work step. In the third column, the times for each active timer are entered.  
Timer 1, Timer 2,  
Timer 3, Timer 4  
6.1.2.1 Measurement process  
The measurement process defines the handling and the  
measurements of the test:  
•
At which and how many wavelengths should measurements be  
completed  
•
•
How many absorbance measurements must be completed?  
When should the zero measurement and the sample  
measurement be completed?  
•
Are waiting times necessary between measurements?  
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Advanced Operations  
•
Should individual program sequences be repeated?  
The elements of a measuring sequence, such as zero and sample  
measurements and the timer(s) (reaction times, waiting times, etc.)  
are individually defined.  
6.1.2.2 Enter a new element of a measuring sequence  
Important Note: Each component of the measurement process  
must be entered in the order in which it will be completed.  
1. Highlight the Measurement Process line in the data overview  
and press Edit.  
2. Press Edit again and then New.  
Content and definition of the keys  
[Z] key / Zeroing  
1. Press the [Z] key to program a zero measurement. Confirm  
with OK.  
2. Press New and then Zeroing... and use the alphanumeric  
keypad to enter the wavelength at which the zero measurement  
is to be completed. Press OK and confirm the input by pressing  
OK again.  
3. If zero measurements are to be carried out at a number of  
wavelengths, repeat the above two steps for each wavelength.  
Note: The entered measurement sequence is displayed.  
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Advanced Operations  
Process Timer key  
1. Press the Process Timer key to enter any waiting, reaction or  
handling times that have to be taken into account. Use the  
alphanumeric keypad to enter the time. Press OK and confirm  
the input by pressing OK again.  
Note: This time is integrated into the measurement process.  
Note: The entered measurement sequence is displayed.  
[R] key / Reading...  
1. Press the [R] key to program a measurement of the substance  
that is to be analyzed. Confirm with OK.  
2. Press New and then Reading... and use the alphanumeric  
keypad to enter the wavelength at which the measurement is to  
be completed. Press OK and confirm the input by pressing OK  
again.  
3. If measurements are to be completed at a number of  
wavelengths, repeat the above two steps for each wavelength.  
Note: The entered measurement sequence is displayed.  
{ } key  
Elements of the measurement sequence that are to be repeated  
are placed in brackets.  
The left bracket "{"marks the start of the sequence that is to be  
repeated and the right bracket "}" marks the end.  
Note: The key showing the right bracket remains inactive until a left bracket  
is entered.  
1. Press {.  
2. Press the key that defines the sequence that is to be repeated:  
[Z] or [R]. Confirm with OK.  
3. Press New and then press Zeroing... or Reading... and use  
the alphanumeric keypad to enter the wavelength at which the  
measurement should be completed. Press OK and confirm the  
input by pressing OK again.  
4. Press } to end the sequence.  
Note: If an action such as a zero measurement recurs at different  
stages of a measurement sequence, the series of actions is numbered  
sequentially (e.g. Z1, Z2, etc.).  
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Delete an element of a measuring sequence  
Select the appropriate line and press Delete. The element is  
deleted.  
Insert an element of a measuring sequence  
Select the line in the measuring sequence where the insertion is be  
made and press New.  
A new element can be entered at the selected position.  
When the input is complete, press OK in the "Measurement  
Process" display. The data overview is then displayed.  
6.1.2.3 Enter the calibration formula (evaluation formula)  
The calibration formula (evaluation formula) defines the calculation  
and display of intermediate and final results. The previously defined  
elements of the measuring sequence are the basis for calculating  
the concentrations.  
Enter calibration formula C1  
1. Highlight the Formula line in the data overview and press Edit.  
2. Highlight the line C1: Off and press Edit.  
3. Select C1: Off again and press Edit. The display changes to  
C1: On.  
4. Highlight the next line C1 = to define the formula and press  
Edit.  
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Advanced Operations  
Refer to Table 11 for detailed information on the Edit  
formula keys.  
Note: The evaluation formula is built up successively in the display in  
accordance with the input.  
Note: The arrow key deletes the most recently entered element of the  
formula.  
Table 11 Edit formula key descriptions  
Key Description  
Screen  
Press the Abs/Variables key to select, from the  
displayed list, the required element of the defined  
Abs/Variables measuring sequence and therefore the corresponding  
measurement wavelength, so that this can be taken into  
account in the formula.  
New Number  
Press New Number to enter a new factor or constant.  
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Table 11 Edit formula key descriptions (continued)  
Screen  
Key  
Description  
Press +– ÷x, to enter a mathematical operation.  
Select the operation and press OK to confirm.  
The available choice of mathematical operations  
depends on the defined formula.  
This means that functions such as "( )" or "ln"/"log" etc.  
are only active if a term in parentheses or the calculation  
of a logarithm is mathematically permissible in the  
defined formula (this also applies to the basic  
mathematical operations).  
The following mathematical operations are available:  
+– ÷x  
•
•
•
•
•
•
•
+ (Addition)  
– (Subtraction)  
÷ (Division)  
x (Multiplication)  
^ (Exponent)  
Ln (Natural logarithm)  
Log (Common logarithm)  
Press >=< to include logic statements/links/conditions in  
the formula. The following functions are available:  
= (Equal to), < (Less than), > (Greater than), <= (Less  
than or equal to), >= (Greater than or equal to), IF,  
THEN, ELSE  
When the evaluation formula C1 has been entered  
completely, press OK to confirm. Press OK again to  
return to the Formula display.  
>=<  
When the formula C1 has been entered and confirmed,  
the parameter name, upper and lower limits of the  
measuring range and display result (yes, no) can be  
entered.  
Enter the next calibration formula (C2 or Cn)  
5. Highlight C2: Off and press Edit.  
6. Select C2: Off again and press Edit. The display switches to  
C2: On.  
7. Highlight the next line C2 = to define the formula and press  
Edit.  
In addition to the keys described in Table 11, only one other  
function is available:  
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Select Conc. key  
If an already defined formula, in this case C1, is to be taken into  
account in the formula for C2, press Select conc.  
Enter the number of the formula (e.g. 1 for C1) and press OK to  
confirm.  
Cn can now be linked with a mathematical operation.  
Note: The Cn concentrations that are to be calculated are numbered in  
sequence: C1, C2, C3, etc.  
Note: When the first Cn formula has been defined, the Formula list is  
automatically extended by Cn+1.  
6.1.2.4 Enter variables  
1. Highlight the Variables line in the data overview and press  
Edit.  
2. Select the variable to be edited, press Edit and use the  
alphanumeric keypad to enter the data specified in the working  
procedure (for F1, F2, l1, U1 etc.). Press OK to confirm each  
entry.  
Abbreviation of Variables:  
F1: Factor 1  
F2: Factor 2  
λ1: Wavelength 1  
U1: Conversion Factor 1 for the first chemical form  
U2: Conversion Factor 2 for the further chemical form etc.  
6.1.2.5 Save a free programming user program  
1. Press Store to save the entered data. The data can be stored  
under any data point (Measurement sequence, Formula, Timer,  
etc.).  
2. Press the PC & Printer icon to send the program data to a  
printer or to a USB memory stick (connect the USB memory  
stick to the USB interface first).  
Note: The test data will be formatted in the preinstalled PrgData folder  
as a .csv and as a .lst file.  
3. To transfer the program data from one instrument to another  
create a new folder on the USB memory stick named dbhl and  
copy the .lst files into this folder (see section 6.8.2 on  
page 106).  
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Advanced Operations  
6.1.3 Select a user program  
1. Press User Programs in the Main Menu to view an  
alphabetical list of user programs with program numbers.  
The User Programs list will appear.  
Note: Use the scroll bar to scroll through the list quickly.  
Note: Highlight the selection by pressing it or press Select by  
Number to search for the program by number.  
2. Press Start to run the program.  
6.1.4 Add, edit and delete user programs from the favorites list  
The most frequently used tests/methods in the User Program menu  
can also be added to the list of favorites to simplify their selection.  
1. Press User Programs in the Main Menu.  
The User Programs list will appear.  
2. Highlight the selection by pressing it or press Select by  
Number to search for the program by number.  
Note: Use the scroll bar to scroll through the list quickly. Use the  
alphanumeric keypad to enter the test number (program number) and  
press OK to confirm.  
3. Press Program Options.  
4. Press Add to Favorites, Edit or Delete and press OK to  
confirm.  
Note: If the stored program is deleted in User Programs, it will also be  
deleted in Favorites Programs.  
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Advanced Operations  
6.1.4.1 Add to Favorites  
1. Press Add to Favorites and press OK to confirm.  
The program is added to the Favorites.  
6.1.4.2 Edit  
1. Press Edit and press OK to confirm.  
An overview of the specifications of the programmed test is  
displayed. More information about the input options is provided  
in the section 6.1.2 on page 72.  
6.1.4.3 Delete  
1. Press Delete and press OK to confirm.  
The program is deleted from the list of User Programs.  
Note: If the stored program is deleted in User Programs, it will also be  
deleted in Favorites Programs.  
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Advanced Operations  
6.2 Favorite Programs  
The most frequently used tests/methods in the Stored Programs  
menu and the User Programs menu can also be added to the list  
of favorites to simplify their selection.  
To add Stored Programs and/or User Programs to the favorites  
list or the favorite programs, see section 6.1.4 on page 79.  
6.2.1 Recall a favorite program  
1. Press Favorite Programs in the Main Menu.  
The Favorite Programs list will appear.  
2. Highlight the selection by pressing it.  
Note: Use the scroll bar to scroll through the list quickly.  
Note: Press Select by Number to search for the program by number. Use  
the alphanumeric keypad to enter the test number (program number) and  
confirm the input by pressing OK.  
3. Press Start.  
6.2.2 Delete a favorite program  
1. Press Favorite Programs in the Main Menu.  
The Favorite Programs list will appear.  
2. Highlight the selection by pressing it.  
Note: Use the scroll bar to scroll through the list quickly.  
Note: Press Select by Number to search for the program by number. Use  
the alphanumeric keypad to enter the test number (program number) and  
confirm the input by pressing OK.  
3. Press Remove Program and press OK to confirm.  
Note: If a Favorite Programs is deleted, it will stay in the User  
Programs or Stored Programs.  
Note: If the stored program is deleted in User Programs, it will also be  
deleted in Favorites Programs.  
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Advanced Operations  
6.3 Standard Addition – monitoring/checking results  
The accuracy of measured values (their correspondence with the  
actual concentration of the analyte in the sample) and their  
precision (correspondence of the measurement results obtained  
from several samples containing the same concentration of the test  
analyte) can be determined or improved using the standard addition  
method.  
This method (also referred to as spiking) serves to identify  
sample-specific interference factors, e.g. substances in the sample  
that falsify the analysis (sample matrix effect), a defective  
measuring instrument or contaminated reagents.  
Method:  
A defined amount (concentration) of a standard solution of the test  
substance is added to the sample. The detection rate should be  
close to 100%.  
Measured value after a standard addition  
Expected value after a standard addition  
------------------------------------------------------------------------------------------------------------  
Detection rate =  
Detection rate  
Conclusion  
Probability that the measurement results are correct  
is high.  
100%  
Assumption: The analysis was falsified by  
substances in the sample (sample matrix effect)  
Test to determine whether a sample matrix effect  
is present:  
< 100%  
Use distilled water instead of the sample. Add  
standard solution as described in the procedure.  
Detection rate  
Conclusion  
Ions in the sample are  
interfering with the  
analysis, causing false  
results to be obtained.  
100%  
No interfering ions -  
consider other  
≠ 100%  
interference factors.  
Measures to identify other interference factors:  
Checklist:  
1. Check if the procedure is completed correctly:  
a. Are the reagents added in the correct order?  
b. Is enough time allowed for color development?  
c. Is the correct glassware in use?  
d. Is the glassware clean?  
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Advanced Operations  
e. Does the test require the sample to be at a certain  
temperature?  
f. Was the pH of the sample in the correct range?  
g. Is the pipette volume correct?  
2. Check the used reagents by repeating the standard addition  
procedure with freshly prepared reagents.  
Detection rate  
Conclusion  
The originally used reagents were defective.  
Check the standard solution:  
100%  
Repeat the standard addition procedure with a  
freshly prepared standard solution.  
Detection rate  
Conclusion  
The originally used  
standard solution was  
defective.  
100%  
If none of these measures resolves the problem, please contact the  
manufacturer or a sales representative.  
6.3.1 Complete a standard addition  
Carry out the standard addition in accordance with the  
corresponding procedure.  
There are two different methods:  
Peak volume (Standard addition):  
Defined volumes of a standard solution are added step by step to  
an already analyzed sample. The sample is measured again after  
each addition.  
Sample volume:  
A defined volume of a standard solution of known concentration is  
added to the volume of sample specified in the procedure and the  
sample is measured after each addition. In most cases three  
different standard solutions are prepared and the procedure is  
repeated for each of them.  
Note: The units and chemical forms used for the sample are used for the  
standard solutions. Be careful to ensure that to use the correct units for  
subsequent entries.  
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Advanced Operations  
Peak volume/sample volume methods  
1. Select Stored Programs in the main menu. Select the required  
program.  
2. Press Start.  
3. Analyze a sample without added standard solution in  
accordance with the instructions in the Procedures Manual.  
When the measurement is complete, leave the sample  
cuvette/cell in the cuvette/sample cell holder.  
4. Press Options>More>Standard Addition.  
An overview of the data of the standard addition procedure is  
displayed.  
5. Press OK to accept the standard values for standard  
concentration, sample volume (total) and standard addition  
volume. Press Edit to change any of these values.  
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6. Press the key of the value to change it. Use the alphanumeric  
keypad to change the value. Press OK to confirm.  
7. Press the keys to enter the standard addition volumes. Use the  
alphanumeric keypad to enter the new data and press OK.  
Description of the table of readings  
•
•
•
The first column shows the standard addition volume. 0 mL  
stands for a sample containing no added standard.  
The second column shows the reading of the samples with and  
without added standard.  
The third column shows the detection rate of the added  
standard.  
Note: The black highlighted line is active.  
8. The reading of the sample in the instrument, without added  
standard, is automatically shown under 0 mL.  
9. Prepare the standard addition solution as described in the  
procedure.  
10. Use the arrow keys to select the first standard addition volume  
in the table and insert the cuvette/cell with the corresponding  
volume of added standard into the cell compartment.  
Press Read.  
Repeat the procedure from point 8 with all the other standard  
addition solutions.  
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Advanced Operations  
11. After all the standard addition solutions have been measured,  
press Graph.  
The regression line through the standard addition data points is  
displayed.  
2
The correlation coefficient r indicates how close the data  
points are to the line.  
If the correlation coefficient = 1, the curve is linear.  
The concentration shown above the curve is the estimated  
concentration of the sample without the added standard.  
Note: In the curve menu, the name on the Curve key switches to  
Table. Press Table to display all the data in the table again.  
12. Press Ideal line to display the relationship between the added  
standard solutions and the ideal line (detection rate 100%).  
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6.4 Single Wavelength (absorbance, concentration and transmittance  
measurements)  
The Single Wavelength mode can be used in three ways. For  
sample measurements at a single wavelength, the instrument can  
be programmed to measure the absorbance, % transmittance or  
concentration of the analyte.  
Absorbance measures the amount of light absorbed by the sample,  
in units of absorbance.  
% transmittance measures the percent of the original light that  
passes through the sample and reaches the detector.  
Turn the concentration factor on to select a specific multiplier for  
converting absorbance readings to concentration. In a graph of  
concentration versus the absorbance, the concentration factor is  
the slope of the line.  
6.4.1 Set up single wavelength mode  
Press Single Wavelength in the Main Menu. Press Options for  
Parameter Setup.  
Table 12 Single wavelength setup options  
Description  
For further Options  
Options  
More  
With the Store On setting, all measurement data are stored automatically. With the Store Off  
setting, no measurement data are stored.  
Store Off/On  
% Trans/Abs  
λ Wavelength  
To switch to % transmittance, concentration or absorbance readings  
To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement  
wavelength. The entered wavelength must be in the range from 190–1100 nm.  
This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed  
(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has  
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement  
program.  
Timer icon  
Concentration Factor  
Multiplication factor for converting absorbance values into concentration values.  
Concentration  
Resolution  
To select the position of the decimal point in the calculated concentration readings.  
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Advanced Operations  
Table 12 Single wavelength setup options (continued)  
Options  
Description  
Single Reading Mode: A reading is only displayed after a measurement has been carried out  
(press Read; standard setting) (see section 6.4.2 on page 89).  
Reading Mode  
Continuous Reading Mode: After the zero measurement, all readings are displayed  
automatically and continuously (see section 6.4.3 on page 89).  
Save as User Program  
Recall Data  
To store the selected parameters as a User Program, see section 6.1 on page 61  
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on  
page 35.  
Instrument Setup  
Basic data of the instrument, see section 5.2 on page 23.  
Concentration factor:  
1. Press Concentration Factor: Off in the Options menu. Press  
On to highlight this feature.  
2. Press the ”Factor“ key and use the alphanumeric keypad to  
enter the factor by which absorbance readings are to be  
multiplied. Press the ”Unit“ key to select the units for  
concentration measurements or to create a new unit.  
3. Press OK to confirm.  
Concentration resolution:  
1. Press Concentration Resolution in the Options menu.  
2. Select the resolution and press OK to confirm.  
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Reading mode:  
1. To highlight the required mode, start by pressing Reading  
mode.  
2. Select the required mode, then press OK, then Return to return  
to the result display.  
6.4.2 Take single wavelength measurements (single reading)  
1. Insert the blank cuvette/cell into the cuvette/sample cell holder.  
Press Zero.  
Note: The Read key is only active after the zero measurement has  
been completed.  
2. Insert the sample cuvette/cell into the cuvette/sample cell  
holder. Press Read.  
3. For data storage, see section 5.3.2 on page 38.  
6.4.3 Take single wavelength measurements (continuous readings)  
1. Insert the blank cuvette/cell into the cuvette/sample cell holder.  
Press Zero.  
Note: In the reading mode ”Continuous“ only the Zero key is shown to  
start the reading. The reading sequence is started automatically.  
2. Insert the sample cuvette/cell into the cuvette/sample cell  
holder.  
3. Press Options and then the Store icon to store the displayed  
data in the Data Log.  
Note: For data storage, see section 5.3.2 on page 38.  
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Advanced Operations  
6.5 Multi-Wavelength mode – measurements at more than one wavelength  
In the multi-wavelength mode, absorbance values can be  
measured at up to four wavelengths and the results can be  
mathematically processed to obtain sums, differences and  
relationships.  
Absorbance measures the amount of light absorbed by the sample,  
in units of absorbance.  
% Transmittance measures the percent of the original light that  
passes through the sample and reaches the detector.  
Turning on the concentration factor allows selection of a specific  
multiplier for converting absorbance readings to concentration. In a  
graph of concentration versus the absorbance, the concentration  
factor is the slope of the line. Concentration is calculated using a  
single factor for each wavelength, which is input by the user.  
6.5.1 Set the reading mode at different wavelengths  
Press Multi Wavelength in the Main Menu. Press Options for  
Parameter Setup.  
Table 13 Multi-wavelength setup options  
Options  
More  
Description  
For further Options  
With the Store On setting, all measurement data are stored automatically. With the Store Off  
setting, no measurement data are stored.  
Store Off/On  
% Trans/Abs  
λ Wavelength  
To switch to % transmittance, concentration or absorbance readings  
To enter the measurement wavelength. Use the alphanumeric keypad to enter the measurement  
wavelength. The entered wavelength must be in the range from 190–1100 nm.  
This functions as a stopwatch. It helps to ensure that the steps of an analysis are correctly timed  
(e.g. reaction times, wait times, etc., can be exactly specified). When the specified time has  
elapsed, an acoustic signal is emitted. The use of the timer has no influence on the measurement  
program.  
Timer icon  
Concentration Factor  
Multiplication factor for converting absorbance values into concentration values.  
Concentration  
Resolution  
To select the position of the decimal point in the calculated concentration readings.  
Absorbance Formula  
Save as User Program  
Calculation basis for evaluating samples  
To store the selected parameters as a User Program, see section 6.1 on page 61  
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Advanced Operations  
Table 13 Multi-wavelength setup options (continued)  
Description  
Options  
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on  
page 35.  
Recall Data  
Instrument Setup  
Basic data of the instrument, see section 5.2 on page 23.  
λ / Absorbance formula:  
1. Press Absorbance Formula.  
2. The formula selected in the top key determines the number of  
wavelength and coefficent keys that will appear below. To  
change the absorbance formula, press the top key, select a  
formula from the displayed list and press OK. When a new  
formula is selected, the number of variables below changes to  
match.  
The following formulas are available:  
K
K
K
K
A
A
A
A
+ K  
+ K  
+ K  
A
A
A
1
1
1
1
1
1
1
1
2
2
2
2
2
2
+ K  
+ K  
A
A
3
3
3
3
+ K  
A
4
4
/ K  
A
2
2
(K  
(K  
A
A
+ K  
+ K  
A
A
) / K  
) / (K  
A
1
1
1
1
2
2
2
2
3
3
A
+ K  
A
)
3
3
4
4
A
A
K
K
refers to the absorbance at wavelength 1  
refers to the absorbance at wavelength 2, etc.  
refers to the coefficient at wavelength 1  
refers to the coefficient at wavelength 2, etc.  
1
2
1
2
Coefficients can be set negative where subtraction is required.  
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3. To change a wavelength, press one of the ”λx:“ keys. Enter the  
desired wavelength coefficient into the numeric keypad.  
Press OK to confirm.  
4. To change a coefficient, press one of the ”K :“ keys. Enter the  
X
desired coefficient into the numeric keypad.  
Press OK to confirm.  
Note: The instrument allows entry of up to 5 significant digits, with a  
maximum of 4 significant digits after the decimal point.  
Concentration factor:  
1. Press Concentration Factor: Off in the Options menu. Press  
On to highlight this feature.  
2. Press the ”Factor“ key to enter the factor by which  
absorbance readings are to be multiplied. Press the ”Unit“ key  
to select the units for concentration measurements or to create  
a new unit.  
3. Press OK to confirm.  
Concentration resolution:  
1. Press Concentration Resolution in the Options menu.  
2. Select the resolution and press OK to confirm.  
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Advanced Operations  
6.5.2 Complete a measurement in the multi wavelength mode  
1. Insert the blank cuvette/cell into the cuvette/sample cell holder.  
Press Zero.  
Note: The Read key does not become active until the zero  
measurement has been completed.  
2. Insert the sample cuvette/cell into the cuvette/sample cell  
holder. Press Read.  
Note: The UV lamp is not switched on until a wavelength in the UV  
spectrum has been entered and the blank reading has been started.  
During the warm-up phase of the UV lamp, the message ”Warming  
up...“ is displayed and the ”UV lamp“ symbol flashes. As soon as the  
UV lamp is ready, the blank reading is carried out.  
Note: Zero and Read are disabled until the cell compartment is  
closed.  
3. For data storage, see section 5.3.1.1 on page 35.  
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Advanced Operations  
6.6 Wavelength Scan mode – recording of absorbance and transmittance  
spectrums  
In the wavelength scan mode, the absorbance of the light in a  
solution over a defined wavelength spectrum is measured.  
The measurement results can be displayed as a curve, as percent  
transmittance (%T) or as absorbance (Abs). The collected data can  
be printed as a table or a curve.  
The data are available for formatting changes. These include  
automatic scaling and zoom functions. Maximum and minimum  
values are determined and shown as a table.  
The cursor can be moved to any point on the curve for the purpose  
of reading off the absorbance or transmittance value and the  
wavelength. The data associated with each data point can also be  
shown as a table.  
6.6.1 Set up the wavelength scan  
Press Wavelength Scan in the Main Menu. Press Options for  
Parameter Setup.  
Table 14 Wavelength scan setup options  
Option  
More  
Description  
For further Options  
To store the scan data  
Store icon  
From the displayed list of stored scans, a record is selected for use as a reference  
scan/superimposed scan. This can be highlighted or shown in the background in comparison  
with the actual measured scan.  
Reference Off/On  
Note: This option is only available when there are stored scans with the same wavelength range  
and step.  
λ
To enter the wavelength spectrum and the scan interval  
Enables the user to switch the display back and forth between the scan data tables  
(wavelength/absorbance) and the graph of the curve.  
Select View  
Note: Select View will be activated after the first reading.  
To select Track or Peak/Valley. The selection for this menu item determines to which points on  
the graph the cursor moves.  
Cursor Mode  
Send Data  
To send Data to a printer, computer or USB memory stick (Type A)  
Integral: On/Off  
The integral gives the area and the derivative of the integral gives the original function  
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Advanced Operations  
Table 14 Wavelength scan setup options (continued)  
Description  
Option  
Scale: In the automatic scaling mode, the y-axis is automatically adjusted so that the total scan is  
displayed.  
The manual scaling mode allows sections of the scan to be displayed.  
Units: Choice of absorbance or transmittance.  
Scale & Units  
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on  
page 35.  
Recall Data  
Instrument Setup  
Basic data of the instrument, see section 5.2 on page 23.  
λ Setting wavelength  
1. Press the λ key in the Options menu to select the wavelength  
range and the wavelength step.  
2. Press the upper left key to open the numeric keypad and select  
the minimum wavelength. Press OK to confirm.  
3. Press the upper right key to open the numeric keypad and  
select the maximum wavelength. Press OK to confirm.  
Note: Do not select the same wavelength for minimum and maximum.  
4. Highlight the required wavelength step.  
Note: Scan recordings of high resolution data take a longer time than  
recordings of low resolution data.Selecting a larger step allows the  
instrument to scan faster, but decreases the resolution of the collected  
data.  
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Advanced Operations  
5. According to the selected wavelength range, select the interval  
from the actively displayed wavelength steps. In summery  
maximally 780 measuring steps can be accomplished during a  
Scan.  
Note: The maximum wavelength adjusts automatically if the difference  
between the maximum and minimum wavelength is not a multiple of  
the interval.  
6. Press OK to return to the scan mode. Selected parameters are  
displayed along the graph‘s x-axis.  
Select view (displaying table)  
1. Press Select View in the Options menu after a reading is  
taken.  
2. A table with the results is displayed.  
3. To return to the graph press Options and then View Graph.  
Cursor mode  
1. Press Cursor Mode: Track in the Options menu.  
2. The selection for this menu item determines what data are  
displayed in the table. Highlight Track or Peak/Valley.  
3. Press OK to confirm.  
4. Press Return to return to the scan mode.  
Integral  
The Integral applies to the whole wavelength range of the scan.  
1. Press Integral: Off in the Options menu.  
2. Highlight On to show the Integral. To find the integral of other  
wavelength ranges, change the wavelength range and scan  
again.  
3. Press OK to confirm.  
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4. Press Return to return to the scan mode.  
Note: The Integral is shown instead of the date on the display.  
Note: For the next scan measurement the setting for the Integral will be  
On.  
Scale & units  
1. Press Scale & Units.  
2. Highlight the required units (Abs or %T).  
3. Highlight Auto or Manual scaling on the graph´s y-axis.  
Note: If manual scaling is selected, use the alphanumeric keypad to set the  
limits y  
and y  
. The graph is adjusted to display only the values in the  
min.  
max.  
selected range. If automatic scaling is selected, the instrument sets the  
limits automatically so that the total range can be displayed.  
4. Press OK.  
5. Press Return to return to the scan mode.  
6.6.2 Wavelength scan reading  
After the scanning parameters have been selected, the baseline  
must be scanned. Changing any of the scanning parameters  
requires a new baseline scan. When the baseline has been  
scanned, the instrument is ready to scan one or more samples.  
1. Press Wavelength Scan in the Main Menu.  
2. Insert the blank cuvette/cell into the cuvette/sample cell holder.  
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3. Press Zero. ”Zeroing....“ appears below the graph as the  
baseline scan begins.  
4. Insert the prepared sample cuvette/cell into the cuvette/sample  
cell holder.  
5. Press Read. Below the graph ”Reading...“ appears and a graph  
of the absorbance or transmittance values at the scanned  
wavelengths is displayed continuously.  
The Wavelength Scan is complete, if  
•
•
•
the graph is shown full size,  
the scaling of the x-axis fits automatically,  
the Cursor functions in the vertical navigation bar are  
highlighted.  
6.6.2.1 Navigation of the wavelength scan graph or a wavelength scan analysis  
Table 15 Navigating the wavelength scan  
Cursor Function/  
Description  
Zoom Function  
Curve Icon  
Choice of Cursor Mode Peak/Valley (cursor moves between minimum/maximum absorbance  
(Choice of Cursor Mode) values) or Cursor Mode Tracking (cursor moves over each data point of the scan).  
The arrow keys are used (right/left) to move the cursor (depending on the selected mode) to the  
next data point. The data of the data point (wavelength/absorbance or transmittance value) are  
highlighted on the x and y axes.  
Arrow keys  
Note: Press any point on the curve to display the associated data.  
This function is used to magnify the section of the curve in the vicinity of the cursor. The original  
curve size can be restored by pressing the zoom icon again.  
Zoom Icon  
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Advanced Operations  
6.6.3 Work with reference scans  
There are two options to work with Reference Scan:  
Option 1:  
1. Press Reference: Off in the Options menu to select another  
scan to display on the same screen with the current scan.  
Highlight the required scan number and press  
Highlight Reference.  
Note: After selecting a reference scan the Reference: Off key in the  
Options menu turns into Reference: On.  
Note: Only scans that have the same wavelength range and step can  
be displayed using the overlay option. A reference curve can only be  
displayed with another active curve. Two previously stored scans  
cannot be overlaid.  
2. The reference curve is shown in gray. The absorbance or  
transmittance value and the associated wavelength are  
highlighted in gray.  
Note: A black and an gray box are shown in the left corner of the  
display. The gray box relates to the reference scan and the black one  
relates to the current wavelength scan.  
3. To complete the wavelength scan reading, see section 6.6.2 on  
page 97.  
•
•
•
The newly plotted wavelength scan curves are shown in  
black.  
The absorbance or transmittance value and the associated  
wavelength are highlighted in black.  
In addition, the display shows the difference between the  
wavelength scan curve and the reference curve against the  
wavelength.  
4. Press the black or gray small box in the left upper corner on the  
screen to switch between the actual wavelength scan and  
reference scan.  
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Advanced Operations  
Option 2:  
1. Insert the blank cuvette/cell into the cuvette/sample cell holder.  
Press Zero.  
2. Insert the sample cuvette/cell into the cuvette/sample cell  
holder. Press Read.  
•
•
The newly plotted wavelength scan curves are shown in  
black.  
The absorbance or transmittance value and the associated  
wavelength are highlighted in black.  
3. Press Options and then Reference: Off in the Options menu  
to select another scan to display on the same screen with the  
current scan. Highlight the required scan number and press  
Highlight Reference.  
Note: After selecting a reference scan the Reference: Off key in the  
Options menu turns into Reference: On.  
Note: Only scans that have the same wavelength range and step can  
be displayed using the overlay option. A reference curve can only be  
displayed with another active curve. Two previously stored scans  
cannot be overlaid.  
4. The reference curve is shown in gray. The absorbance or  
transmittance value and the associated wavelength are  
highlighted in gray.  
•
Additionally the difference of the extinction and/or  
transmission value between the two Scans (measured  
Scan and reference Scan) is indicated/highlighted at each  
position of the cursor.  
Note: A black and an gray box are shown in the left corner of the  
display. The gray box relates to the reference scan and the black one  
relates to the current wavelength scan.  
5. Press the black or gray small box in the left upper corner on the  
screen to switch between the actual wavelength scan and  
reference scan.  
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6.7 Time course of absorbance/transmittance  
The Time Course Mode is used to collect data in either absorbance  
or transmittance for a user-specified length of time. After the data  
are collected, they can be displayed in either graphic or tabular  
format.  
6.7.1 Time course setup parameters  
Press Time Course mode in the Main Menu. Press Options to  
configure parameters.  
Table 16 Time course setup options  
Description  
Option  
More  
For further Options  
Store icon  
To store the scan data  
To input the total time for data collection and the time interval between the collection of data  
points  
Time & Interval  
λ
To input the wavelength setting  
To display readings in absorbance, transmittance or concentration. This can be changed after  
sample data are collected  
View Table/Graph  
Scale: In the automatic scaling mode, the y-axis is automatically adjusted so that the total scan is  
displayed.  
The manual scaling mode allows sections of the scan to be displayed.  
Units: Choice of absorbance or transmittance.  
Scale & Units  
Send Data  
To send Data to a printer, computer or USB memory stick (Type A)  
Call up saved measurement data, wavelength scans or time courses, see section 5.3 on  
page 35.  
Recall Data  
Instrument Setup  
Basic data of the instrument, see section 5.2 on page 23.  
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Time & interval:  
1. Press Time & Interval in the Options menu.  
2. Input the total time and the reading time and press OK to  
confirm.  
Note: In total 500 measuring steps are possible. To select a total time and  
a time interval that would cause this number of measurements to be  
exceeded, the time interval is defined automatically and the OK key is  
inactivated.  
Scale & units:  
1. Press Scale & Units in the Options menu.  
2. Highlight Abs or %T as the required units.  
3. Highlight Auto or Manual scaling on the graph´s y-axis.  
Note: If manual scaling is selected, use the alphanumeric keypad to set the  
limits y  
and y  
. The graph is adjusted to display only the values in the  
min.  
max.  
selected range. If automatic scaling is selected, the instrument sets the  
limits automatically so that the total range can be displayed.  
4. Press OK to confirm.  
5. Press Return to return to the scan mode.  
6.7.2 Time course scan reading  
After the parameters have been selected, the instrument must be  
blanked, then the sample can be analyzed.  
1. Insert the blank cuvette/cell into the cuvette/sample cell holder.  
Press Zero. The blank reading is shown on the display.  
2. Insert the sample cuvette/cell into the cuvette/sample cell  
holder. Press Read. Start collecting time course (kinetic) data.  
Note: During the measurement the Zero and Read keys change to  
Mark and Stop.  
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•
•
Select Mark to mark the next data point collected. This mark is  
not used by the instrument, but is available for the user and  
may indicate a significant event, such as the addition of a  
sample or other reagent. The mark is also shown in the table.  
Select Stop to stop taking sample readings.  
6.7.3 Analysis of time course data  
After the data are collected, the following manipulations can be  
done on the graphic data:  
The Time Course Program is complete, if  
•
the sound is turned on, the instrument beeps when the  
readings are done  
•
•
•
the graph is shown fullsize,  
the scaling of the x-axis occurs automatically,  
the Cursor functions in the vertical navigation bar are  
highlighted.  
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6.7.3.1 Navigation of a time scan or a time scan analysis  
After a time scan has been completed, the time and the  
absorbance/transmittance data are displayed as a curve.  
Where the cursor is positioned on the curve, the elapsed time up to  
this point and the corresponding absorbance are highlighted.  
Table 17 Navigating the time scan  
Cursor Function/  
Zoom Function  
Description  
Delta mode: A second cursor is highlighted. The position of the fixed cursor was previously  
defined in Cursor Mode Single. Use the active cursor to select any point on the measurement  
curve. The difference to the fixed cursor is shown on the curve. The delta values are  
correspondingly highlighted and displayed on the x and y axes.  
Curve Icon  
(Choice of Cursor Mode)  
2
The gradient of the curve and the correlation coefficient (r ) between the cursor points in the  
Delta mode are shown under the curve.  
Cursor Mode Single: The cursor moves to each selected measurement point of the scan.  
The arrow keys (right/left) are used to move the cursor (depending on the selected mode) to the  
next data point. The data of the data point (wavelength/absorbance or transmittance value) are  
highlighted on the x and y axes.  
Arrow keys  
Zoom Icon  
Note: Press any point on the curve to display the associated data.  
This function is used to magnify the section of the curve in the vicinity of the cursor. The original  
curve size can be restored by pressing the zoom icon again.  
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Advanced Operations  
6.8 System checks  
1. Press System Checks in the Main Menu.  
The System Checks menu contains instrument information and  
various performance tests.  
6.8.1 Instrument information  
1. Press Instrument Information in the System Checks menu.  
2. The model, serial number and software version are displayed.  
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6.8.2 Upgrade of the instrument software  
To obtain the software for the update from the Internet at  
www.hach.com:  
1. Go to http://www.hach.com.  
2. On the DR 5000 product page, click Lab System  
Software/Software Update Downloads under the heading  
Downloads.  
3. Locate the appropriate download and follow the prompts for  
saving the file(s) to the USB memory stick.  
4. On the DR 5000, press Instrument Update in the System  
Checks menu.  
5. Connect the USB stick to the USB interface on the DR 5000  
(section 3.3 on page 14). Press OK.The link is established  
automatically and the software is updated.  
6. Press OK to return to the System Checks menu.  
Note: When the instrument software has been updated, a prompt to restart  
the instrument is displayed.  
6.8.3 Optical checks  
For each optical check, the measured results must be evaluated  
against user-specific requirements. The check options do not  
define tolerances.  
1. Press Optical Checks in the System Checks menu.  
The Optical Checks menu contains programs for checking the  
wavelength accuracy, stray light and photometric accuracy.  
An optional test filter set (Verification Kit) (Section 9 on page 129)  
containing six precision glass filters, target values, tolerances and  
instructions is available and is recommended as an aid for carrying  
out comprehensive in-house instrument checks.  
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Advanced Operations  
6.8.3.1 Wavelength check  
The Wavelength Check test is used to check wavelength accuracy  
at 656.1 and 486.0 nm and to check bandwidth at 656.1 nm, only.  
1. Press Wavelength Check in the Optical Checks menu.  
2. Press λ to select the wavelength.  
3. Make a choice and confirm with OK.  
4. Press Start.  
Note: During the warm-up phase of the UV lamp, the message ”Lamp  
Warmup...“ is displayed.  
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5. The result is displayed.  
6. Press Cancel to return to Optical Checks.  
6.8.3.2 Noise check  
The Noise Check test is used to test the photometric noise in the  
instrument.  
This test can be used to test noise at any wavelength and at an  
absorbance level determined by a specific sample.  
1. Press Noise Check in the Optical Checks menu.  
2. Press λ to input the wavelength.  
3. Input the wavelength and press OK to confirm.  
4. Press Zero.  
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5. Insert sample cuvette/cell into the cell compartment. Close the  
cell compartment.  
6. Press Start.  
7. The result will be displayed. Thirty readings are averaged for  
the blank. The ”Average“ and ”Std Deviation“ are calculated  
from 100 consecutive absorbance readings (Noise, Sample,  
Reference).  
Note: Failings and passings will be defined by the user.  
8. Press PC & Printer icon to send data to a PC or Printer.  
9. Press Cancel to return to Optical Checks.  
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6.8.3.3 Stray light check  
The Stray Light test is used to measure the stray light in the  
instrument at 220.0 nm and 340.0 nm.  
1. Press Stray Light Check in the Optical Checks menu.  
2. Press λ to select the wavelength.  
3. Make a choice and confirm with OK.  
4. Press Zero.  
5. Insert the sample cuvette/cell or reference filter into the cell  
compartment. Close the cell compartment. Press Start.  
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6. The ”Average“ and ”Std Deviation“ are calculated from  
100 successive absorbance measurements. The result is  
displayed and is to be compared with the nominal/standard  
data (given in the quality control certificate) of the sample  
cell/test filter.  
Note: Failings and passings will be defined by the user.  
7. Press Cancel to return to Optical Checks.  
6.8.3.4 Absorbance check  
The Absorbance Check test is used to test the photometric  
accuracy and repeatability of the instrument.  
This test can be used to test absorbance at any wavelength by a  
specific sample or test filter set (Refer to section 6.8.3.6 on  
page 113).  
1. Press Absorbance Check in the Optical Checks menu.  
2. Press λ to input the wavelength.  
3. Input the wavelength and press OK to confirm.  
4. Remove any cuvette/sample cell from the cell compartment,  
close the cell compartment, and press Zero.  
5. Insert sample cuvette/cell or test filter into the cell  
compartment, close the cell compartment, and press Read.  
6. Repeat steps 4 and 5 for a total of 10 replicates to obtain final  
results.  
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7. The result is displayed and is to be compared with the  
nominal/standard data of the test filter. If a specific sample is  
used, the measured results must be evaluated against  
user-defined requirements.  
8. Press Cancel to return to Optical Checks.  
6.8.3.5 Drift check  
The Drift Check test is used to test the stability of the instrument.  
Note: The Drift Check runs 1 hour.  
1. Press Drift Check in the Optical Checks menu.  
2. Press λ to input the wavelength.  
3. Input the wavelength and press OK to confirm.  
4. Press Start.  
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Advanced Operations  
5. The Drift Check runs 1 hour.  
This test takes a reading every minute for one hour. Every  
15 minutes, linear regression is used to calculate the slope  
(rate of change) for the previous 15 minute interval.  
6. The last reading and slope for each 15 minute interval are  
displayed. At the end of the hour, the overall values are  
calculated and displayed. The test proceeds to completion.  
7. Press Cancel to return to Optical Checks.  
6.8.3.6 Verification kit  
The Verification Kit, LZV 537, (see Section 9 on page 129) is  
designed for periodic monitoring of scattered light, photometric  
accuracy and the wavelength accuracy of the spectrophotometers.  
When results exceed allowable tolerances (given in the quality  
control certificate), contact the manufacturer.  
1. Press Verification Kit in the Optical Checks menu.  
2. Press Nominal Values.  
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Advanced Operations  
3. Press Edit.  
An automatic menu guidance queries values (filters,  
wavelength, nominal values and tolerances) given in the quality  
control certificate, to the following specifications:  
•
•
•
Stray Light  
Photometrical accuracy  
Wavelength accuracy  
4. Press OK when all values are entered and the overview is  
displayed.  
5. Press Verification.  
6. Insert the Multi Cell Holder in the cell compartment in such a  
way that the cell opening for 10 mm square cuvettes/cells is at  
the front. Secure the holder with two locking screws.  
7. Remove any cuvettes/cells from the cell compartment, close  
the cell compartment, and press Start.  
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Advanced Operations  
8. Insert the different filter in the given order one after the other  
with the filter glass facing the front of the instrument. Press  
Next after inserting a filter and closing the cell compartment  
After the last measurement the results are displayed.  
9. Press PC & Printer icon to send the data to a USB memory  
stick, PC or to a printer.  
The files will be stored automatically as CSV file (Comma  
Separated Value). The file name will be formatted as  
“Verification.csv“.  
6.8.4 Output checks  
6.8.5 Lamp history  
If a printer is connected a test printing of the current screen will be  
printed.  
The Lamp History menu provides the following information:  
•
•
•
The amount of time that the lamp has been on (Hours)  
The number of times that the lamp has been turned on (Cycles)  
The number of times that the instrument tried to turn the UV  
lamp on (triggers)  
After a lamp is replaced and the Lamp History is reset, the display  
of the total operating time is reset to 0.  
1. Press Lamp History in the System Checks menu.  
2. Press Reset VIS and the Visible Lamp will be reset.  
3. Press Reset UV and the UV Lamp will be reset.  
4. Press OK to return to System Checks.  
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Advanced Operations  
6.8.6 Factory service  
The Factory Service menu is password protected. This menu is not  
intended for customer use.  
6.8.7 Service time  
In order to ensure a regular inspection, an automatic memory  
reference for the service times can be entered. After switching the  
instrument on this memory reference will be activated and indicated  
at the appropriate time.  
1. Press Service Time in the System Checks menu.  
2. Select On and then Last Service to enter the date of the last  
inspection.  
3. Press OK to confirm.  
4. Select Next Service to determine a specific period of time up to  
the next inspection.  
5. Press OK to confirm.  
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Advanced Operations  
If the next service is due, the message "Next service is due!" is  
displayed after switching on the instrument.  
6. Press OK to return to the Main Menu.  
Contact the manufacturer or distributor to arrange an appointment  
for the next service.  
6.8.8 Instrument Backup  
Before the next service date the Instrument Backup menu offers  
the possibility to store all programs, measuring data, Operator ID,  
Sample ID, passwords and all adjustable data on a USB stick.  
1. Press Instrument Backup in the System Checks menu.  
2. Connect the USB memory stick (section 3.3 on page 14).  
3. Press Store to start a Backup.  
Note: If the USB stick is not connected, the message "Please insert USB  
Memory" is displayed. Connect a USB stick, in order to store the data.  
Press OK to confirm and press Store again.  
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Advanced Operations  
Note: If the Backup was already stored before, the message "Data already  
exists. Overwrite?" is displayed. Press OK to overwrite the data.  
If the file was stored the message Instrument Backup is stored to  
USB stick will be displayed.  
4. Press OK to return to the System Checks menu.  
Restore backup data:  
Important Note: All current data will be overwritten when restoring  
the Backup file!  
1. Press Instrument Backup in the System Checks menu.  
2. Connect the USB memory stick containing the Backup  
(section 3.3 on page 14).  
3. Press Restore to pass back the data.  
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Advanced Operations  
Note: If the USB stick is not connected, the message "Please insert USB  
Memory" is displayed. Connect a USB stick, in order to store the data.  
Press OK to confirm and press Restore again.  
4. Press OK to confirm after the message “Instrument Backup  
from S/N XXXXXXX. Restore?“ is displayed.  
5. After the backup start the instrument again.  
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Advanced Operations  
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Section 7 Maintenance  
CAUTION  
Potential Chemical, Biological Eye and Skin Hazards.  
Only qualified personnel should conduct the tasks described  
in this section of the manual.  
Important Note: Remove any cuvettes/cells that are still in the  
instrument and dispose of them or their contents using an approved  
disposal method.  
7.1 Cleaning requirements  
CAUTION  
Potential Pinch, Eye, Burn and Chemical Hazards.  
Always disconnect power from the instrument before  
attempting any cleaning operations.  
Important Note: Under no circumstances should the instrument,  
display or the accessories be cleaned with solvents such as white  
spirit, acetone, etc.  
7.1.1 Spectrophotometer  
•
Clean the enclosure, cuvette/sample cell compartments and all  
accessories with a soft damp cloth. A mild soap solution can  
also be used. Do not get excess water in the cuvette/sample  
cell compartments. Do not insert a brush or sharp object into  
Cell Compartment #1 to avoid damaging the mechanical  
components.  
•
Dry the cleaned parts carefully with a soft cotton cloth.  
7.1.2 Display  
•
Take care not to scratch the display. Do not touch the screen  
with ball pens, pencils or similar pointed objects.  
•
Clean the display with a soft, lint-free and oil-free cotton cloth.  
Diluted window cleaner liquid can also be used.  
7.1.3 Cuvettes/sample cells  
CAUTION  
Potential Chemical/ Biological Exposure Hazards.  
Use proper laboratory practices whenever there is a risk of  
chemical exposure.  
1. After performing a procedure, clean glass cuvettes/sample cells  
with cleaning agents.  
2. Afterwards, rinse the cuvettes/sample cells several times with  
tap water and then thoroughly with deionized water.  
Important Note: Glass cuvettes/sample cells that have been used  
for organic solvents (such as chloroform, benzene, toluene, etc.)  
must be rinsed with acetone before being treated with cleaning  
agents. In addition, another rinse with acetone is necessary as a  
final treatment step before the cuvettes/sample cells are dried.  
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Maintenance  
7.2 Lamp replacement  
CAUTION  
The UV lamp generates UV light. Do not look directly at an  
operating lamp without wearing UV protective eye glasses.  
The Lamp compartment is on the left side behind the display and is  
provided with ventilation on the back side. The tungsten and  
deuterium (UV) lamp are installed in the lamp compartment. On the  
back side a fan is installed for cooling of electric components.  
The ventilation system operates automatically.  
Refer to section 7.2.1 on page 122, section 7.2.2 on page 123 and  
Figure 8 on page 124 for instructions on replacing the tungsten  
lamp and deuterium lamp (UV).  
7.2.1 Tungsten lamp replacement  
1. Switch the instrument off.  
2. Unplug the power cord.  
DANGER  
The instrument must remain switched off until the change of  
lamp has been completed.  
WARNING  
Burn Hazard. Wait until the lamp cools down. Contact with the  
hot lamp can cause burns.  
3. Use a screwdriver to remove the cover from the back of the  
instrument (the screws may be slotted or cross-headed).  
4. Place the cover and the attached fan carefully beside the  
instrument (take special care with the fan cable).  
5. Push the spring (Figure 8 on page 124, item 2) up and remove  
the tungsten lamp (Figure 8, item 1) from the lamp  
compartment.  
6. Unplug plug contact (Figure 8, item 7) from the tungsten lamp.  
Important Note: The lamp should only be held by the lamp socket.  
Avoid touching the glass.  
7. Push plug contact firmly onto the new tungsten lamp.  
8. Insert the tungsten lamp into the lamp compartment. Push the  
spring down.  
9. Check that the spring and the lamp socket are positioned  
correctly.  
10. Use a screwdriver to screw the back cover onto the instrument  
again.  
11. Plug in power supply again.  
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Maintenance  
12. Switch the instrument on.  
13. Reset the Lamp History, see section 6.8.5 on page 115.  
7.2.2 Deuterium lamp (UV) replacement  
1. Switch the instrument off.  
2. Unplug the power cord.  
DANGER  
The instrument must remain switched off until the change of  
lamp has been completed.  
WARNING  
Burn Hazard. Wait until the lamp cools down. Contact with the  
hot lamp can cause burns.  
3. Use a screwdriver to remove the cover from the back of the  
instrument (the screws may be slotted or cross-headed).  
4. Place the cover and the attached fan carefully beside the  
instrument (take special care with the fan cable).  
5. Unplug the deuterium lamp (Figure 8 on page 124, item 4) from  
the socket by pushing down on the safety contact.  
6. Use a screwdriver to unscrew the two fastening screws  
(Figure 8, item 3) (the screws may be slotted or cross-headed)  
out of the socket.  
7. Holding the lamp socket, lift the deuterium lamp up out of the  
lamp compartment (remove the complete unit, including the  
cable).  
8. Carefully insert the new deuterium lamp into the lamp  
compartment.  
Important Note: Do not touch the glass envelope on the new lamp.  
If it is touched, clean with alcohol.  
9. Screw in both fastening screws again until they are finger-tight.  
10. Insert the deuterium lamp in the socket so that the safety  
contact clicks into place.  
11. Use a screwdriver to screw the back cover onto the instrument  
again.  
12. Plug in power supply again.  
13. Switch the instrument on.  
14. Reset the Lamp History, see section 6.8.5 on page 115.  
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Maintenance  
Figure 8 Lamp compartment  
1
2
3
4
Tungsten lamp  
Spring  
5
6
7
Plug contacts for fan  
Plug contact for deuterium lamp  
Screws  
Plug contact for tungsten lamp (with grip and cable)  
Deuterium lamp (lamp socket)  
7.3 Fuse replacement  
DANGER  
Certain electrical circuits within this equipment are protected  
by fuses against over-current conditions. For continued  
protection against a risk of fire, replace fuses only with the  
same type and rating specified.  
DANGER  
Failed fuses are generally an indication of a problem with the  
equipment. If fuse failure continues, contact a service  
representative for instructions on how to return the equipment  
for repair. Do not attempt to repair the equipment yourself.  
1. Switch the instrument off.  
2. Unplug the power cord to remove all power from the  
instrument.  
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Maintenance  
3. Pull out the plastic base over the power cable socket.  
4. Remove the defective fuse, see Figure 1 on page 14.  
5. Insert new fuse (T 2 A H; 250 V)  
6. Replace the plastic base.  
7. Plug in the power cord.  
8. Switch the instrument on.  
7.4 Filter pad maintenance  
To determine when the filter mat needs to be replaced, inspect the  
filter mat every 3–6 months (in a relatively dust-free environment,  
this interval can be longer).  
1. Remove any cuvettes and cuvette/sample cell adapter from the  
cell compartment.  
2. Turn the instrument off.  
3. Unplug the power cord.  
4. Lift the instrument and check the color of the filter mat. Replace  
the filter mat if is dark gray or black.  
7.4.1 Filter pad replacement  
5. Carefully turn the instrument over and place it on a soft surface.  
6. Use a screwdriver (standard or cross-head) to open the  
filter grid (Figure 9 on page 126, item 1).  
7. Lift the filter grid (Figure 10 on page 126, item 1).  
8. Remove the old air filter pad and replace it with a new air filter  
pad (Figure 10, item 2).  
9. Screw the grid back in place.  
10. Carefully stand the instrument upright.  
11. Plug the instrument in.  
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Maintenance  
Figure 9 Base of DR 5000 with filter grid  
Phillips screw  
1
2
Filter grid  
Filter pad  
3
Figure 10 Change of filter pad  
1
Filter pad  
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Section 8 Troubleshooting  
Problem/Display screen  
Absorbance > 3.5!  
Likely Cause  
Action  
Dilute the sample and repeat the  
measurement  
The measured absorbance exceeds 3.5  
Calculated concentration is higher than Dilute the sample and repeat the  
Concentration too high!  
999999  
measurement  
Error  
Barcode controlnumber?  
Please update program data!  
Deviation to the stored data  
Data updating  
Error  
Data updating  
Program not available.  
Please update program data!  
Barcode cuvette test missing  
Error  
Clean Cuvette  
The cuvette is soiled or there are  
undissolved particles in the cuvette  
Clean the cuvette; allow the particles to  
settle  
Error  
Check the lamp and replace it if  
necessary  
The lamp output is too low  
Please check the UV Lamp.  
Error  
Check the lamp and replace, if  
necessary.  
Selfcheck stopped.  
Please check the lamp.  
Please close the lid.  
Error [xx]  
Self Check Test stops while starting the  
instrument  
Close the lid.  
Press Start Again.  
Error  
Remove the cuvette/sample cell from  
the cell compartment.  
Selfcheck stopped.  
Please remove the cuvette  
Please close the lid.  
Self Check Test stops while starting the  
instrument  
Press OK.  
Kink in tube  
Error Sipper?  
Check the tube connections  
Peristaltic pump failure  
Error  
Contact the manufacturer or a sales  
representative and indicate the error  
number  
Selfcheck stopped.  
Hardware error.  
Error [x]  
Electronic defect  
Negative result!  
The calculated result is negative  
Check the concentration of the sample  
Check the programming  
Error in the test database / user  
database  
No evaluation!  
Contact the manufacturer or a sales  
representative  
The measured absorbance is above the Dilute the sample and repeat the  
Over measuring range  
Under measuring range  
calibration range of the test  
measurement  
If possible, select a test with a lower  
measurement range or use a cuvette  
with a longer path length  
The measured absorbance is below the  
calibration range of the test  
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Section 9 Replacement Parts  
Description  
Cat.-No.  
Sipper Module DR 5000  
LZV485  
LZV513  
LZV479  
A23618  
A23620  
LZV582  
LZV566  
LZY274  
LZV659  
A23800  
LZV580  
LZV780  
LZV569  
2946900  
LZV567  
A23778  
A23792  
A23772  
A23766  
Peltier temperature controlled Module DR 5000  
Pour-Thru Module DR 5000  
Multi-Cell Holder DR 5000  
Carousel Holder (Sample changer)  
USB-Keyboard (keyboard layout: US)  
USB-Barcode Scanner (handset scanner for entering sample IDs)  
Hach Data Trans (PC software for data transfer)  
Brewery Analysis Software  
Sipper Tubing, Inlet and Waste  
Sipper Tubing, PHARMED quality  
Sipper Tubing, comes standard with Sipper Module  
Pour Thru Tube Kit  
USB-Memory Stick  
USB-Interface Cable, (1 m)  
Tungsten (VIS) Lamp  
Deuterium (UV) Lamp  
Fuse  
Filter Pad  
Certified test filter set for self-checks (Verification Kit)  
(6 precision glass filters with target values)  
LZV537  
Semi-Micro Cell, 50 mm, Glass  
LZP269  
LZP331  
2095100  
4822800  
2629250  
2624450  
2629201  
2624401  
4864302  
2495402  
2665902  
2410212  
A24209  
LZV510  
LZV649  
5913700  
LZY421  
LZV728  
Macro Cell, 20 mm, Glass  
Sample Cell, 1 cm Square Glass, (10 mm path length) matched pair  
Sample Cell, 1 cm Square Quartz, (10 mm path length) matched pair  
Sample Cell, 50 mm Rectangular Glass  
Sample Cell, 50 mm Rectangular Quartz  
Sample Cell, 100 mm Rectangular Glass  
Sample Cell, 100 mm Rectangular Quartz  
Sample Cell, 1" Round Plastic, (1 cm path length) 10 mL, w/cap  
Sample Cell, 1" Square Glass, 10 mL, matched sets  
Sample Cell, 1" Square Glass, 10&25 mL, matched sets  
Sample Cell, 1" Square Plastic  
Pour Thru Cell, 1x1 cm (Quartz/160 µL/ Glass center height 10 mm)  
Pour Thru Cell, 1x1 cm (Quartz/450 µL/ Glass center height 10 mm)  
Pour Thru Cell, 5x1 cm (Quartz/370 µL/ Glass center height 10 mm)  
Pour Thru Cell, 1" Square (1" path length)  
Adapter for 100 mm Sample Cell  
Dust Cover  
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Replacement Parts  
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Section 10 Contact Information  
HACH Company  
World Headquarters  
P.O. Box 389  
Loveland, Colorado  
80539-0389 U.S.A.  
Tel (800) 227-HACH  
(800) -227-4224  
(U.S.A. only)  
Repair Service in the  
United States:  
HACH Company  
Ames Service  
100 Dayton Avenue  
Ames, Iowa 50010  
Tel (800) 227-4224  
(U.S.A. only)  
Repair Service in Canada:  
Hach Sales & Service  
Canada Ltd.  
1313 Border Street, Unit 34  
Winnipeg, Manitoba  
R3H 0X4  
Tel (800) 665-7635  
(Canada only)  
Repair Service in  
Latin America, the  
Caribbean, the Far East,  
Indian Subcontinent, Africa,  
Europe, or the Middle East:  
Hach Company World  
Headquarters,  
P.O. Box 389  
Fax (970) 669-2932  
orders@hach.com  
www.hach.com  
Fax (515) 232-3835  
Tel (204) 632-5598  
Fax (204) 694-5134  
canada@hach.com  
Loveland, Colorado,  
80539-0389 U.S.A.  
Tel +001 (970) 669-3050  
Fax +001 (970) 669-2932  
intl@hach.com  
HACH LANGE GMBH  
HACH LANGE LTD  
HACH LANGE LTD  
HACH LANGE GMBH  
Willstätterstraße 11  
Pacific Way  
Salford  
GB-Manchester, M50 1DL  
Tel. +44 (0)161 872 14 87  
Fax +44 (0)161 848 73 24  
info@hach-lange.co.uk  
www.hach-lange.co.uk  
Unit 1, Chestnut Road  
Western Industrial Estate  
IRL-Dublin 12  
Tel. +353(0)1 46 02 5 22  
Fax +353(0)1 4 50 93 37  
info@hach-lange.ie  
www.hach-lange.ie  
Hütteldorferstr. 299/Top 6  
A-1140 Wien  
Tel. +43 (0)1 9 12 16 92  
Fax +43 (0)1 9 12 16 92-99  
info@hach-lange.at  
www.hach-lange.at  
D-40549 Düsseldorf  
Tel. +49 (0)2 11 52 88-320  
Fax +49 (0)2 11 52 88-210  
info@hach-lange.de  
www.hach-lange.de  
DR. BRUNO LANGE AG  
Juchstrasse 1  
HACH LANGE FRANCE  
S.A.S.  
HACH LANGE SA  
Motstraat 54  
DR. LANGE NEDERLAND  
B.V.  
CH-8604 Hegnau  
33, Rue du Ballon  
B-2800 Mechelen  
Laan van Westroijen 2a  
NL-4003 AZ Tiel  
Tel. +31(0)344 63 11 30  
Fax +31(0)344 63 11 50  
info@hach-lange.nl  
www.hach-lange.nl  
Tel. +41(0)44 9 45 66 10  
Fax +41(0)44 9 45 66 76  
info@hach-lange.ch  
www.hach-lange.ch  
F-93165 Noisy Le Grand  
Tél. +33 (0)1 48 15 68 70  
Fax +33 (0)1 48 15 80 00  
info@hach-lange.fr  
Tél. +32 (0)15 42 35 00  
Fax +32 (0)15 41 61 20  
info@hach-lange.be  
www.hach-lange.be  
www.hach-lange.fr  
HACH LANGE APS  
HACH LANGE AB  
HACH LANGE S.R.L.  
HACH LANGE S.L.U.  
Åkandevej 21  
Vinthundsvägen 159A  
SE-128 62 Sköndal  
Tel. +46 (0)8 7 98 05 00  
Fax +46 (0)8 7 98 05 30  
info@hach-lange.se  
www.hach-lange.se  
Via Riccione, 14  
I-20156 Milano  
Tel. +39 02 39 23 14-1  
Fax +39 02 39 23 14-39  
info@hach-lange.it  
www.hach-lange.it  
Edif. Arteaga Centrum  
C/Larrauri, 1C- 2ª Pl.  
E-48160 Derio/Vizcaya  
Tel. +34 94 657 33 88  
Fax +34 94 657 33 97  
info@hach-lange.es  
www.hach-lange.es  
DK-2700 Brønshøj  
Tel. +45 36 77 29 11  
Fax +45 36 77 49 11  
info@hach-lange.dk  
www.hach-lange.dk  
HACH LANGE LDA  
HACH LANGE SP.ZO.O.  
HACH LANGE S.R.O.  
HACH LANGE S.R.O.  
Av. do Forte nº8  
Fracção M  
ul. Opolska 143 a  
Lešanská 2a/1176  
Roľnícka 21  
SK-831 07 Bratislava –  
Vajnory  
Tel. +421 (0)2 4820 9091  
Fax +421 (0)2 4820 9093  
info@hach-lange.sk  
www.hach-lange.sk  
PL-52-013 Wrocław  
Tel. +48 (0)71 342 10-83  
Fax +48 (0)71 342 10-79  
info@hach-lange.pl  
www.hach-lange.pl  
CZ-141 00 Praha 4  
Tel. +420 272 12 45 45  
Fax +420 272 12 45 46  
info@hach-lange.cz  
www.hach-lange.cz  
P-2790-072 Carnaxide  
Tel. +351 214 253 420  
Fax +351 214 253 429  
info@hach-lange.pt  
www.hach-lange.pt  
HACH LANGE KFT.  
Hegyalja út 7-13.  
H-1016 Budapest  
HACH LANGE S.R.L.  
Str. Leonida, nr. 13  
Sector 2  
HACH LANGE  
8, Kr. Sarafov str.  
BG-1164 Sofia  
HACH LANGE SU  
ANALİZ SİSTEMLERİ  
LTD.ŞTİ.  
Tel. +36 (06)1 225 7783  
Fax +36 (06)1 225 7784  
info@hach-lange.hu  
www.hach-lange.hu  
RO-020555 Bucuresti  
Tel. +40 (0) 21 201 92 43  
Fax +40 (0) 21 201 92 43  
info@hach-lange.ro  
www.hach-lange.ro  
Tel. +359 (0)2 963 44 54  
Fax +359 (0)2 866 04 47  
info@hach-lange.bg  
www.hach-lange.bg  
Hilal Mah. 75. Sokak  
Arman Plaza No: 9/A  
TR-06550 Çankaya/ANKARA  
Tel. +90 (0)312 440 98 98  
Fax +90 (0)312 442 11 01  
bilgi@hach-lange.com.tr  
www.hach-lange.com.tr  
HACH LANGE D.O.O.  
ΗΑCH LANGE E.Π.Ε.  
HACH LANGE E.P.E.  
Fajfarjeva 15  
SI-1230 Domžale  
Tel. +386 (0)59 051 000  
Fax +386 (0)59 051 010  
info@hach-lange.si  
www.hach-lange.si  
Αυλίδος 27  
27, Avlidos str  
GR-115 27 Αθήνα  
Τηλ. +30 210 7777038  
Fax +30 210 7777976  
info@hach-lange.gr  
www.hach-lange.gr  
GR-115 27 Athens  
Tel. +30 210 7777038  
Fax +30 210 7777976  
info@hach-lange.gr  
www.hach-lange.gr  
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Contact Information  
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Section 11 Limited Warranty  
Hach Company warrants its products to the original purchaser against any defects that are due to faulty  
material or workmanship for a period of one year from date of shipment unless otherwise noted in the  
product manual.  
In the event that a defect is discovered during the warranty period, Hach Company agrees that, at its  
option, it will repair or replace the defective product or refund the purchase price excluding original  
shipping and handling charges. Any product repaired or replaced under this warranty will be warranted  
only for the remainder of the original product warranty period.  
This warranty does not apply to consumable products such as chemical reagents; or consumable  
components of a product, such as, but not limited to, lamps and tubing.  
Contact Hach Company or your distributor to initiate warranty support. Products may not be returned  
without authorization from Hach Company.  
Limitations  
This warranty does not cover:  
•
Damage caused by acts of God, natural disaster, labor unrest, acts of war (declared or undeclared),  
terrorism, civil strife or acts of any governmental jurisdiction  
•
•
•
•
•
•
Damage caused by misuse, neglect, accident or improper application or installation  
Damage caused by any repair or attempted repair not authorized by Hach Company  
Any product not used in accordance with the instructions furnished by Hach Company  
Freight charges to return merchandise to Hach Company  
Freight charges on expedited or express shipment of warranted parts or product  
Travel fees associated with on-site warranty repair  
This warranty contains the sole express warranty made by Hach Company in connection with its  
products. All implied warranties, including without limitation, the warranties of merchantability and fitness  
for a particular purpose, are expressly disclaimed.  
Some states within the United States do not allow the disclaimer of implied warranties and if this is true in  
your state the above limitation may not apply to you. This warranty gives you specific rights, and you  
may also have other rights that vary from state to state.  
This warranty constitutes the final, complete, and exclusive statement of warranty terms and no person  
is authorized to make any other warranties or representations on behalf of Hach Company.  
Limitation of Remedies  
The remedies of repair, replacement or refund of purchase price as stated above are the exclusive  
remedies for the breach of this warranty. On the basis of strict liability or under any other legal theory, in  
no event shall Hach Company be liable for any incidental or consequential damages of any kind for  
breach of warranty or negligence.  
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Limited Warranty  
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Index  
A
M
Absorbance Check .................................................111  
Absorbance Formula .........................................90, 91  
Alphanumeric keypad .............................................. 21  
Audio signals ........................................................... 25  
B
Main Menu ............................................................... 22  
Maintenance .......................................................... 121  
Mark ....................................................................... 103  
Measurement Process .................................54, 55, 72  
Measuring sequence ................................................ 73  
Multi Wavelength ...............22, 64, 66, 90, 91, 92, 93  
N
Barcode Programs .......................................51, 52, 53  
edit ..................................................................... 53  
Basic data ................................................................ 42  
C
Calibration formula ................................................... 75  
Calibration Settings .................................................. 66  
Chemical Form ............................................43, 47, 53  
Chemical form ....................................................51, 52  
Cleaning  
Name ....................................................................... 54  
Noise Check ........................................................... 108  
O
Operating environment ............................................ 13  
Operator ID .............................................................. 23  
creating .............................................................. 23  
delete ................................................................. 23  
Optical Checks ...... 106, 107, 108, 110, 111, 112, 113  
Output Checks ........................................................115  
P
Password ................................................................. 32  
activate .............................................................. 32  
PC and Printer ...................................................29, 31  
Print data .................................................................. 31  
Printer Setup ............................................................ 29  
Printing data ............................................................. 31  
Program ................................................................... 61  
Programm overview ................................................. 22  
R
Reading Mode ....................................... 43, 44, 88, 89  
Reagent Blank ...................................................43, 48  
Recall Data ................22, 38, 43, 53, 88, 91, 95, 101  
Reference Scan .................................................94, 99  
S
Safety Information ...................................................... 9  
Sample blank ........................................................... 53  
Sample ID ................................................................ 24  
create ................................................................ 24  
delete ................................................................. 24  
Save as User Program .......................... 43, 53, 88, 90  
Scale & Units .................................... 95, 97, 101, 102  
Security List ............................................................. 32  
Select View .............................................................. 96  
Send Data .....................................36, 43, 53, 94, 101  
Service Time ...........................................................116  
Single Reading ......................................................... 43  
Single Wavelength .......22, 43, 45, 63, 66, 87, 88, 89  
Software ...........................................................58, 106  
Standard Addition ..............................................43, 82  
Standard Adjust .................................................43, 46  
Stored Data .............................................................. 35  
Stored Programs ..........22, 42, 43, 45, 46, 47, 48, 49  
send Data .......................................................... 43  
Stray Light Check ....................................................110  
System Check .........................................................116  
System Checks 22, 105, 107, 108, 110, 111, 112, 113,  
................................................................ 115, 116, 117  
Display ............................................................. 121  
Spectrophotometer .......................................... 121  
Concentration Factor ............................ 87, 88, 90, 92  
Concentration Resolution ..................... 87, 88, 90, 92  
Continuous Reading ..........................................43, 45  
Cursor Mode ......................................................94, 96  
Cuvettes/cells ........................................................ 121  
D
Data  
delete ...........................................................35, 37  
recall .................................................................. 35  
send ................................................................... 35  
store .................................................................. 35  
Data Storage ............................................................ 38  
Date ......................................................................... 25  
Date and Time ......................................................... 25  
Dilution Factor ..............................................43, 45, 53  
Display .............................................................21, 121  
Display and Sound ................................................... 25  
Drift Check ..............................................................112  
F
Factory Service .......................................................116  
Favorites ............................................... 22, 49, 80, 81  
delete ...........................................................80, 81  
edit ..................................................................... 80  
recall .................................................................. 81  
Filter Settings ........................................................... 36  
Formula ..............................................................54, 55  
Free programming ..........................72, 73, 75, 78, 80  
H
HACH Data Trans ..............................................31, 36  
Hazard Information .................................................... 9  
I
Instrument Backup ..........................................117, 118  
Instrument Information ........................................... 105  
Instrument Setup .......22, 23, 43, 53, 88, 91, 95, 101  
Integral ...............................................................94, 96  
Interfaces ................................................................. 29  
Internet .............................................................58, 106  
L
Language ................................................................. 19  
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T
Time & Interval ...............................................101, 102  
Time Course ..........22, 38, 39, 40, 41, 101, 103, 104  
Time Course Scan Reading ................................... 102  
Timer ..................................25, 43, 53, 54, 56, 87, 90  
Touch Screen .......................................................... 21  
U
Unpack the instrument ............................................. 13  
Update .......................................................54, 58, 106  
User Programs ...................................................22, 61  
V
Variables ......................................................54, 56, 78  
Verification Kit .........................................................113  
Version ...............................................................54, 55  
View Table ............................................................. 101  
W
Warranty ................................................................ 133  
Wavelength ........................................................87, 90  
Wavelength Check ................................................. 107  
Wavelength Range .................................................. 95  
Wavelength Scan .........22, 38, 39, 40, 41, 94, 95, 98  
Wavelength setting ................................................ 101  
Wavelength spectrum .............................................. 94  
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