GE Camera Accessories UV 1 User Manual

Monitor UV-1  
User Manual  
59-7797-01  
Edition AG  
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Important user information  
Reading this entire manual is  
recommended for full  
understanding and use of  
this product.  
Warranty and Liability  
GE Healthcare Bio-Sciences AB guarantees that the  
product delivered has been thoroughly tested to  
ensure that it meets its published specifications.  
The warranty included in the conditions of  
delivery is valid only if the product has been  
installed and used according to the instructions  
supplied by GE Healthcare Bio-Sciences AB.  
GE Healthcare Bio-Sciences AB shall in no event be liable  
for incidental or consequential damages,  
including without limitation, lost profits, loss of  
income, loss of business opportunities, loss of  
use and other related exposures, however  
caused, arising from the faulty and incorrect use  
of the product.  
The exclamation mark within an equilateral  
triangle is intended to alert the user to the  
presence of important operating and  
maintenance instructions in the literature  
accompanying the instrument.  
Trade marks  
FPLC® is the exclusive trade mark of  
GE Healthcare Bio-Sciences AB.  
In view of the risk of trade mark  
Should You have any comments on this manual,  
degeneration, it is respectfully suggested that  
authours wishing to use this designation refer to  
its trade mark status at least once in each article.  
Copyright© 1995 GE Healthcare  
we will be pleased to receive them at:  
GE Healthcare Bio-Sciences AB  
S-75182 Uppsala  
Sweden  
Bio-Sciences AB  
GE Healthcare Bio-Sciences AB reserves the right to  
changes in the specifications without prior  
make  
notice.  
All rights reserved. No part of this publication  
may be reproduced, stored in a retrieval system  
or transmitted in any form by any means,  
without permission in written form from the  
company.  
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Contents  
1. Introduction ........................................................................................4  
2. General Description ..........................................................................5  
2.1 Basic principle.............................................................................5  
2.2 Optical unit .................................................................................7  
2.3 Control unit .................................................................................9  
3. Installation ........................................................................................11  
3.1 Site requirements ......................................................................11  
3.2 Unpacking .................................................................................11  
3.3 Electrical connections ..............................................................11  
3.4 Installation of the filter ............................................................12  
3.5 Installation of the flow cell .....................................................13  
3.6 Connecting the optical unit ....................................................14  
3.7 Connecting a recorder .............................................................14  
4. Operation ..........................................................................................15  
4.1 Choice of wavelength ..............................................................15  
4.2 Choice of AU or transmission (%T).......................................15  
4.3 Conversion table T% to AU and OD .....................................16  
4.4 Start-up ......................................................................................16  
4.5 Stabilization time......................................................................16  
4.6 Basic operating procedure - AU .............................................17  
4.7 Basic operating procedure - Transmission (%T) ..................17  
4.8 Shut down .................................................................................17  
5. Maintenance .....................................................................................18  
5.1 General precautions .................................................................18  
5.2 Cleaning the flow cell ..............................................................18  
5.3 Changing the flow cell ............................................................19  
5.4 Interference filters ....................................................................20  
5.5 Other optical surfaces ..............................................................20  
5.6 Instrument housing .................................................................20  
5.7 Lamp and optical system test .................................................20  
5.8 Changing the mercury lamp ...................................................21  
5.9 Mercury lamp - maximum light adjustment ........................22  
6. Trouble-shooting ..............................................................................23  
7. Technical Specifications ..................................................................24  
8. Accessories and Spare Parts...........................................................25  
3
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1. Introduction  
1. Introduction  
The UV-1 is a fixed wavelength UV monitor, consisting of a control  
unit and an optical unit that can be positioned up to 10 meters apart.  
A mercury lamp is the stable light source, furthermore a built-in  
reference cell eliminates baseline drift. The output signal can be recor-  
ded in either AU or %T. Sensitivity range between 0.01 and 2 AUFS,  
or (0-100%T).  
The UV-1 offers a range of three detection wavelengths:  
254, 280 and 405 nm. In addition, five flow cells are available for  
different applications in Standard Chromatography, FPLC and  
Industrial applications.  
4
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2. General Description  
2. General Description  
GE Healthcare LKB Monitor UV-1 consists of an optical unit  
containing the flow cell, lamp, filter assemblies and preamplifiers, and a  
control unit containing the signal processing circuits. The two units are  
connected via a multi-core cable hardwired from the control unit to  
the optical unit. Connection to the recorder and the mains supply is  
made via the control unit.  
2.1 Basic principle  
The single path Monitor UV-1 is a dual beam instrument with a  
sample cell and a reference cell. Optical path lengths are 10 mm,  
3 mm, or 2 mm depending upon the flow cell chosen.  
Flow cell S-2 has only the sample cell.  
Note:  
The sample cell and reference cell are angled to receive light from the  
same point in the lamp (Fig. 1) thus assuring a stable base-line by  
negating the effects of variations in lamp intensity.  
The lamp is powered by a stabilized DC to AC converter operating at  
20 kHz to maximize its efficiency. Lamp output is independent of  
variations in the line voltage.  
Light from the lamp passes through an aperture (254 nm, 405 nm) or  
a fluorescence converter (280 nm) and through the reference cell and  
sample cell. The light output from each cell passes through an  
interference filter and falls onto a solid state photo-detector whose  
output is a linear function of the light intensity (Fig. 1 and Fig. 2).  
Fig. 1. Optical path.  
5
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2. General Description  
Fig. 2 Block diagram  
The photocurrent from each detector is amplified in a pre-amplifier  
before passing to the signal processing circuitry in the control unit.  
If transmission is to be monitored, the signal passes directly to the  
low pass filter. If AU is to be monitored, the reference cell light  
intensity (IR) is compared with the sample cell intensity (IS) to form  
log (IR/ IS) in a logarithmic circuit before passing to the low-pass filter.  
The signal finally passes to the range selector before being presented  
to the output terminal.  
6
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2. General Description  
Front panel controls  
2.2 Optical unit  
5
4
1
2
3
Fig. 3. Optical unit. Front panel.  
No. Item  
Description  
1
Cell holder  
The complete cell holder is removed by turning the  
locking (Fig. 4:6) knob on the rear panel  
2
3
4
Sample inlet  
Inlet for sample flow  
Outlet for sample flow  
Sample outlet  
Reference inlet  
Inlet for flowing reference liquid. The reference cell  
may be operated dry, with static reference liquid  
5
Reference outlet  
Outlet for flowing reference liquid  
7
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2. General Description  
Rear panel controls  
6
11  
9
Guide pin  
Screw  
7
10  
8
Fig. 4. Optical unit. Rear panel.  
No. Item  
Description  
6
Locking knob  
The cell holder is in locked position when the locking  
knob is turned fully in the direction of the arrow  
7
8
Filter inlet  
Filters, converters or apertures are inserted in the  
positions indicated. When inserting them, align  
the arrowhead on the end of the filter or converter  
Aperturewith the arrowhead next to the appropriate  
opening. Filters, converters and apertures must be  
pressed fully  
Converter or  
9
Support rod  
Enables the optical unit to be mounted on laboratory  
scaffolding. The rod may be secured in either of two  
positions by a screw and a guide-pin  
10 Support rod  
Alternative position for support rod  
11 Multi-core cable  
Connects the optical unit to the control unit via  
an 11-pin plug with a snap-lock. For disconnection  
squeeze the ribbed sides of the plug firmly and pull  
Warning:  
The optical unit contains a UV-source which is exposed  
if the cover is removed.  
8
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2. General Description  
Front panel controls  
2.3 Control unit  
15  
16  
12  
13  
14  
Fig. 5. Control unit. Front panel.  
No. Item  
Description  
12  
Absorbance range Selector for the desired absorbance range  
selector  
0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1 or 2 AU full scale  
deflection. In the position SHORT, the signal output  
terminals are disconnected from the rest of the control  
circuitry and short-circuited. This position is useful when  
setting zero on the recorder.  
13  
Mode switch  
AU or %T  
The AU / % T switch, enables the UV-1 to be used  
to monitor the absorbance or transmission of a flowing  
liquid. When the UV-1 is used in the AU mode, each of  
the other position corresponds to the optical  
absorbance which will give a full scale deflection (fsd)  
on a recorder with a sensitivity of 10 mV. Absorbances  
are measured relative to air or a liquid which is present  
in the reference cell  
When the recorder is used together with UV-1 in the  
% T mode, the absorbance range selector switch is  
used, together with baseline adjust, to adjust the  
recorder response corresponding to 100 % T  
14  
Baseline adjust  
Ten-turn potentiometer to adjust the recorder baseline  
Indicates power is on  
15  
16  
Indicator lamp  
Mains switch  
The UV-1 is turned on by switching this knob in the ON  
(upper) position. The indicator lamp will light to show  
that the mains voltage is on  
9
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3. Installation  
3. Installation  
UV-1 should be installed on a stable, flat surface away from all sour-  
ces of vibration. The atmosphere should be free of both excess humi-  
dity and corrosive or contaminated vapours which may form deposits  
on the component in the optical path.  
3.1 Site  
requirements  
UV-1 can be installed either in a coldroom or at ambient temperature  
in the laboratory. To minimise drift, the temperature should be kept  
constant. UV-1 optical unit should be positioned away from all sour-  
ces of draught, heat and direct sunlight. UV-1 should be placed away  
from any compressor and the fan stream from coldboxes and col-  
drooms.  
The UV-1 may be operated ambient temperatures in the range  
0-40 °C (20-30 °C at full specifications).  
One mains power point is required to operate UV-1. Separate power  
points are required for all ancillary equipment, such as recorder.  
The power consumption of the monitor is max 20 VA.  
Note:  
It is important that the interference filters and flow cells  
should not be handled during unpacking. For protection of  
these items they should remain in their packing materials  
until required for use.  
3.2 Unpacking  
Carefully unpack the UV-1. Check the contents against the packing  
list supplied. Inspect for any damage that may have occurred during  
transit. Report any damage immediately to the local GE Healthcare  
representative and to the transport company concerned. Save  
the packing material if future transport can be foreseen.  
The instrument is supplied with mains cables and fuses for both  
100-130 V and 220-240 V operation.  
3.3 Electrical  
connections  
1.  
Ensure that the mains switch (Fig. 5:16) on the front panel of  
the control unit is in the OFF position.  
2.  
Select the correct value of fuse from the fuse kits supplied.  
For 110-130 V operation, use the 250 mA fuse supplied.  
For 220-240 V operation, use the 125 mA fuse supplied.  
Insert the fuse into the fuse cap, and then fit the fuse cap into the  
fuse holder on the rear panel (Fig. 6:18) of the instrument.  
3.  
Check that the mains voltage selector (Fig. 6:17) on the rear  
panel of the control unit is set to the mains voltage in the  
laboratory. If necessary, turn with a thick bladed screwdriver the  
mains voltage setting until appropriate setting is indicated in the  
small window.  
Note:  
Use the 220 V setting for a 230 V mains outlet.  
11  
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3. Installation  
Fuse  
Mains voltage  
Voltage selector setting  
250 mA  
250 mA  
125 mA  
125 mA  
110 V  
110  
130  
220  
240  
130 V  
220-230 V  
240 V  
4.  
Select the mains cable corresponding to your mains outlet.  
Discard unwanted mains cable immediately. Connect the  
instrument to a grounded mains outlet.  
Note:  
Do NOT switch on.  
Note:  
Special care must be taken when handling interference  
filters. DO NOT touch the filter surface. The filters should  
not allowed to come in contact with any liquid or exposed  
to temperatures above 60 °C. For directions on cleaning  
interference filters, see Section 5.4  
3.4 Installation  
of the filter  
1.  
Select the appropriate filter for the wavelength to be used.  
Insert the filter and converter or aperture in the optical unit  
(Fig. 4:7, Fig. 4:8). Each filter is marked with its wavelength and  
the letter F. The 280 nm converter is marked 280 C. The  
aperture for use with the 254 nm or 405 nm filters is marked 0.  
2.  
The filter and converter or aperture must be pushed fully home.  
Wavelength  
Filter  
Converter or Aperture  
254 F  
280 F  
405 F  
0
254 nm  
280 nm  
405 nm  
280 C  
0
12  
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3. Installation  
Monitor UV-1 accepts flow cells for Standard Chromatography, FPLC  
and industrial applications.  
3.5 Installation of  
the flow cell  
Product  
Material of  
Path  
Total dead Illuminated Pressure Application  
Code No.  
wetted parts  
length  
volume  
volume  
limit  
area  
S-2  
Fluoro-plastic,  
2 mm  
10 mm  
3 mm  
80 µl  
2 µl  
0.3 MPa  
(3 bar)  
Standard  
Chromatography  
19-4840-02 optical quartz  
HR-10 Fluoro-plastic,  
24 µl  
50 µl  
250 µl  
8.7 µl  
3 µl  
1.0 Mpa  
(10 bar)  
FPLC, Standard  
Chromatography  
19-6254-02 optical quartz,titanium  
3 mm Fluoro-plastic,  
1.0 MPa  
(10 bar)  
Preparative FPLC,  
Standard Chromatography  
19-2503-02 optical quartz  
10 mm Fluoro-plastic,  
19-2504-02 optical quartz  
10 mm  
5 mm  
8.7 µl  
1.0 MPa  
(10 bar)  
Standard  
Chromatography  
5 mm  
Industrial  
Silicon rubber,  
polypropylene,  
0.2 MPa* Industrial scale  
(2 bar)  
19-4510-02 optical quartz  
* Flow rate 300 I/h at 0.1 MPa  
All flow cell are mounted in the holders, ready to be installed directly  
into the cell housing. Release the locking knob (Fig. 4:6) by turning it  
against the direction of the arrow and insert the flow cell in its holder  
into the optical unit. Lock it in position by turning the locking knob  
fully in the direction of the arrow.  
For more information please refer to the instruction sheet supplied  
with the respectively flow cell.  
Fig. 7. S-2 flow cell  
Fig. 8. HR-10 flow cell  
Fig. 9. 3 mm flow cell  
Fig. 10. 10 mm flow cell  
Fig. 11. Industrial flow cell  
13  
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3. Installation  
The optical unit may be placed on the bench or mounted on  
laboratory scaffolding. For scaffolding mounting, mount the support  
rod on the optical unit. The rod may be mounted horizontally or  
vertically (Fig. 4:9). Tighten the the Allen screw firmly. The optical  
unit should be placed as close as possible to the column outlet.  
Connect the cable from the optical unit to the 1 l-pin socket on the  
back of the control unit (Fig. 6:21). The plug has a snap lock.  
To remove it, squeeze the ribbed sides firmly and pull.  
3.6  
3.7  
Connecting  
the optical unit  
There is one 10 mV signal output port on the rear panel of the  
control unit. It is for use with GE Healthcare recorders or similar  
instruments. Connect the output terminals to the input of the  
recorder, using a signal cable (Fig. 12). Connect the shield of the  
signal cable to the grounded terminal port on the rear panel of the  
control unit. Choose the 10 mV input range on the recorder for full  
scale response.  
Connecting a  
recorder  
Fig. 12. Connections between the control unit and a dual channel recorder  
Recorder REC 102.  
14  
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4. Operation  
4. Operation  
The UV-1 can be operated at either 254 nm, 280 nm or 405 nm.  
The choice of wavelength will depend on the spectral properties of  
both the eluent and the substances to be detected. Proteins and poly-  
pep-tides containing aromatic amino acids are usually best detected at  
280 and 254 nm. Nucleic acids and poly-nucleotides are usually best  
detected at 254 nm and ferroproteins i.e. hemoglobins, cytochrome  
and porphyrin derivatives at 405 nm.  
4.1 Choice of  
wavelength  
The UV-1 may be set to monitor either the absorbance or %  
transmittance of a flowing liquid. Absorbance measurements give  
recorder responses which are proportional to the solute concentration  
when Lambert Beers Law is obeyed. The AU setting is thus most  
appropriate for general use, particularly when quantitative results are  
required. However, even with the 3 mm path length cell, there is  
always a slight risk that the peak will go off scale. Under these  
conditions, peak maxima can still be located by monitoring  
transmission.  
4.2 Choice of AU or  
transmission  
(%T)  
The relationship between AU and Optical density (OD) is  
AU = L x OD  
where L is the optical path length in cm.  
The relationship between OD and transmission expressed as %T is  
100  
T % =  
.
anti log10 (L OD)  
where L is the optical path length in cm.  
15  
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4. Operation  
4.3  
Conversion  
table T% to AU  
and OD  
T%  
AU  
OD  
(3 mm cell)  
OD  
(10 mm cell)  
1
5
2.000  
1.301  
1.000  
0.824  
0.699  
0.602  
0.523  
0.500  
0.456  
0.398  
0.347  
0.301  
0.260  
0.222  
0.200  
0.186  
0.155  
0.125  
0.100  
0.097  
0.070  
0.050  
0.046  
0.022  
0.020  
0.010  
2.000  
1.301  
1.000  
0.824  
0.699  
0.602  
0.523  
0.500  
0.456  
0.398  
0.347  
0.301  
0.260  
0.222  
0.200  
0.186  
0.155  
0.125  
0.100  
0.097  
0.070  
0.050  
0.046  
0.022  
0.020  
0.010  
6.667  
4.337  
3.333  
2.747  
2.330  
2.007  
1.743  
1.667  
1.520  
1.327  
1.157  
1.003  
0.867  
0.740  
0.667  
0.620  
0.517  
0.417  
0.333  
0.323  
0.233  
0.167  
0.153  
0.073  
0.067  
0.033  
10  
15  
20  
25  
30  
32  
35  
40  
45  
50  
55  
60  
63  
65  
70  
75  
79  
80  
85  
89  
90  
95  
95.5  
97.7  
Note:  
Always ensure that all liquid passing through the flow cell  
are degassed to prevent any air bubble formation in the cell.  
Liquids must be filtered to remove any particulate material  
and prevent any blockage.  
4.4 Start-up  
1.  
Check that the monitor is correctly installed and that the sample  
cell is filled with the appropriate eluent. The reference cell may  
be left closed with air in the cell unless the UV-1 is used with  
eluents showing appreciable UV-absorption. In this case the  
reference cell should be filled with eluent.  
2.  
3.  
If the instrument has been switched off, turn on the mains switch  
(Fig. 5:16) and refer to Section 4.5, to restabilize the instrument.  
Check that the appropriate filter and converter or aperture are in  
place and fully inserted.  
At normal laboratory temperatures the UV-1 requires 2 hours to  
stabilize sufficiently. When the UV- 1 is in constant use, it is  
recommended that it remains switched on. UV- 1 can be switched off  
when not in use for periods of one week or more.  
4.5  
Stabilization  
time  
For coldroom operation below 10 °C, install UV-1 in the coldroom at  
the desired running temperature at least 12 hours before the start of a  
run. This is necessary to allow the instrument housing to equilibrate  
to the temperature of the coldroom. Once equilibration has taken  
place, the stabilisation times given for normal temperatures are valid.  
16  
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4. Operation  
1.  
Switch AU/ %T to AU (Fig. 5:13).  
4.6 Basic  
operating  
2.  
Set the range selector (Fig. 5:12) to SHORT and zero the  
recorder with the recorder zero control.  
procedure -  
AU  
3.  
4.  
Set the range selector to 2 and adjust the recorder baseline with  
the baseline adjust (Fig. 5:14).  
Set the range selector to the appropriate range and readjust the  
recorder baseline with the baseline adjust (Fig. 5:14). Only  
minor adjustment should be necessary.  
1.  
Switch AU to %T (Fig. 5:13).  
4.7 Basic  
2.  
Set the range selector (Fig. 5:12) to SHORT and zero the  
recorder.  
operating  
procedure -  
Transmission  
(%T)  
Turn the baseline adjust (Fig. 5:14) fully clockwise.  
3.  
4.  
Set the range selector so that the recorder gives a deflection just  
greater than 100%.  
Adjust baseline to bring the pen back to 100%.  
5.  
6.  
Exchange the converter for the shutter and zero the recorder  
with the recorder zero. This response will correspond to 0%  
transmission.  
7.  
1.  
Replace the shutter with the converter. This response will  
correspond to 100% transmission. Slight adjustment of baseline  
may be required to obtain the response obtained in Step 5.  
On completion of the chromatographic run, flush the flow cell  
either with pure solvent or the buffer used in the  
4.8 Shut down  
chromatographic run. To prevent the deposition of salts from  
aqueous buffers, flush the cell with distilled water after use, if  
necessary after disconnecting the column.  
Note:  
Never allow aqueous buffers to dry out in the cell. Either  
continue to flush through with buffer or leave the cell filled  
with distilled water.  
2.  
Leave the UV-1 switched on. The monitor should only be  
switched off if it is not going to be used again for more than one  
week.  
17  
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5. Maintenance  
5. Maintenance  
To ensure trouble free running, users are advised to observe the follo-  
wing precautions:  
5.1 General  
precautions  
All liquids passing through the flow cell should be free of  
suspended particles.  
All liquids should be degassed to prevent air bubble formation in  
the flow cell.  
Never allow buffer solutions to dry out in the cell. Always rinse  
the flow cell thoroughly with distilled water after use.  
Handle interference filters with care. Never touch the optical  
surfaces or expose them to temperatures above 60 °C.  
For trouble free operation of the UV-1, it is essential that the flow cell  
is free of any particulate matter and contaminant films. Ensure that  
the flow cell is never allowed to dry out without having been tho-  
roughly rinsed. Liquids containing dissolved salts, proteins or other  
solutes will dry out, leaving contaminants on the inner optical surface  
of the flow cell.  
5.2 Cleaning the  
flow cell  
The cells may be inspected for particles by removing the cell holder  
and examining the light paths with a magnifying glass.  
If the cell contains trapped particles proceed as follows:  
1.  
Remove the cell holder from the optical unit.  
2. Connect a syringe to the outlet tubing and squirt a clean solution  
of ethanol in distilled water (50% v/ v) through the cell in small  
aliquots. Examine the cell from time to time to see when the  
particles have been washed out.  
Rinse the cell with particle-free distilled water (about 100 ml)  
and replace it in the optical unit.  
3.  
4. Reconnect the cell holder to the system to be monitored.  
Most non-particulate contaminants e.g. denaturated proteins, salts  
etc. can be removed by flushing the cell with the appropriate solvent.  
Finally, rinse the cell thoroughly with distilled water or clean solvent.  
Oily deposits, which increase the tendency to trap bubbles, can be  
removed by rinsing the flow cell first with a non-polar solvent  
(e.g. hexane), then with a polar solvent (e.g. isopropanol) and finally  
with distilled water or with detergent see procedure described on next  
page.  
18  
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5. Maintenance  
Cleaning with detergent:  
Remove the cell holder from the optical unit.  
1.  
Pump undiluted cleaning detergent through the cell for at least  
2 hours.  
2.  
Rinse the cell with  
3.  
4.  
a) distilled water (100 ml)  
b) ethanol/ distilled water (50% v/ v, 100 ml)  
c) distilled water (100 ml)  
Replace the cell in the optical unit and reconnect the system to  
be monitored.  
Cleaning with chromic acid:  
Prepare fresh chromic acid by adding concentrated sulphuric  
acid (100 ml) to a saturated solution of sodium dichromate  
(3.5 ml).  
1.  
Chromic acid is extremly corrosive. Treat spills  
immediately with a large excess of water.  
Warning:  
Remove the cell holder from the optical unit.  
2.  
3.  
Connect a glass syringe to the outlet side of the cell and carefully  
draw chromic acid into the cell. Do not draw acid into the  
syringe.  
Allow the acid to remain in the cell for 10-20 minutes. Longer  
exposures (several hours) will not harm the cell.  
4.  
Eject the cleaning solution carefully without splashing and rinse  
the cell with  
5.  
a) distilled water (100 ml)  
b) ethanol/ distilled water (50% v/ v, 100 ml)  
c) distilled water (100 ml)  
Replace the cell in the optical unit and reconnect the system to  
be monitored.  
6.  
To change the flow cell, follow the instructions below.  
1. Switch the UV-1 off at the control unit.  
5.3 Changing the  
flow cell  
2. Disconnect the cell from the system being monitored and empty  
it of liquid.  
Remove the cell holder from the optical unit.  
3.  
4. Remove the black cover by undoing the screw on top of the cell  
holder. The tubing connections to the cell are now accessible.  
Note their positions (Fig. 13).  
Loosen the connections using the tool provided. Do not undo.  
The cell may now be slipped out.  
5.  
6.  
7.  
Before inserting the new cell make sure the tubing ends do not  
protrude into the cell compartment.  
19  
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5. Maintenance  
8. Insert the new cell, being careful not to touch either of the optical  
surfaces. See that the inlet and outlet ports are correctly aligned.  
9. Retighten the screw washers with the tool provided. Do not use  
excessive force.  
10. Check for leakage.  
11. Replace the black cover.  
Sample out  
Reference out  
Sample in  
Reference in  
Fig. 13. Flow cell interior showing tubing connections.  
For optimum performance, it is essential that the interference filters  
are clean and free of any particulate material. Do not touch the inter-  
ference filters. Should the filter become contaminated with dust,finger  
prints or oil, proceed as follows:  
5.4 Interference  
filters  
-
Carefully take out the filter without touching or scratching the  
surface.  
- Use lens cleaning tissue dipped in ethanol to gently clean both  
sides of the filter surface.  
-
Place the clean filter back to the UV-1 or its box.  
- Interference filters should never be exposed to temperatures  
above 60 °C  
Clean all other optical surfaces by wiping the surface with clean, lint-  
free cloth, moistened with carbon tetrachloride, ethanol, or another  
suitable pure solvent.  
5.5 Other optical  
surfaces  
Wipe the instrument regularly with a damp cloth. Let the instrument  
dry completely before use.  
5.6 Instrument  
housing  
Before performing this test see that the filter is clean, that the cell is  
clean and free of bubbles or particles and that the cell holder is  
correctly inserted and locked in position.  
5.7 Lamp and  
optical system  
test  
1. Insert the aperture and filter for 254 nm operation or the  
converter and filter for 280 nm operation.  
2. Set AU / %T to % T.  
3. Set the recorder to the 100 mV range.  
20  
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5. Maintenance  
4.  
Set the range selector to SHORT and zero the recorder.  
5. Set the range selector as follows:  
254 nm, range 1  
280 nm, range 0.2  
6. Turn Zero fully clockwise.  
Response 8 mV or greater: the lamp is operating properly and the  
optical system is clean.  
Response 0 mV or very close to 0 mV: Contact a GE Healthcare  
representative.  
Response less than 8 mV but not very close to zero: check that the  
cell and filter is clean. If fault persists, the filter or lamp may require  
changing (see Section 5.7).  
The low pressure mercury lamp has an expected lifetime of approx.  
8000 hours. Before changing the lamp, carry out the tests described in  
Section 5.6. If the tests indicates an aging lamp, proceed as follows.  
5.8 Changing the  
mercury lamp  
l.  
Disconnect the control unit from the mains supply and  
disconnect the optical unit from the control unit.  
Warning:  
The control unit must be disconnected from the mains  
and from the optical unit. If the UV-Iamp is broken  
make sure that all mercury is removed.  
2. Remove the cell holder.  
3. Remove the two screws and locking washers which secure the  
case of the optical unit to the bottom of the chassis.  
4. Loosen the two Philips screws until the top of the case can be  
lifted clear of the catches.  
5. Pull the case forward, and remove it (Fig. 14).  
Lamp Screw  
Screw  
Stop ring  
Fig. 14. Optical unit interior with lamp driver circuit board removed  
21  
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5. Maintenance  
6. Use an Allen key (2.5 mm) to unscrew the upper of the two  
screws securing the lamp holder and the lamp. Note carefully the  
positions of the insulation sleeve and washer.  
7. Disconnect the lamp from the PC-board.  
8. Bend the upper part of the lamp holder slightly (2-3 mm)  
upwards and withdraw the mercury lamp from it by gently  
pulling the metal socket.  
9. Insert the new mercury lamp and connect it to the PC-board.  
10. Remount the upper lamp holder screw, ensuring that the  
insulations are correctly mounted, reinstall the cell holder and  
perform the maximum light adjustment described in Section 5.8.  
Mercury lamp  
5.9  
- maximum  
light  
adjustment  
Warning:  
During this adjustment stray light may escape from the  
UV source and protective glasses must be worn.  
1.  
2.  
3.  
Connect the optical unit to the control unit and plug into the  
mains supply.  
Switch on the instrument and allow to warm up for about  
10 minutes.  
Insert a filter and the corresponding converter or aperture.  
4. Connect the control unit to a recorder with a full scale response  
of 50 mV.  
Set the range selector to SHORT and zero the recorder.  
5.  
Set AU/ %T to % T and turn the baseline adjust fully clockwise.  
6.  
7. Select a range giving a pen deflection of approx. 50 % and then  
rotate the lamp around its longitudinal axis until maximum pen  
deflection occurs.  
8. Set the range selector to SHORT and adjust the recorder zero to  
bring the pen to the centre of the chart. Mark this pen position.  
Set the range selector to 2 and set AU/ % T to AU.  
9.  
10. Adjust the baseline control to its mid-position, five full turns  
from either end of its range, by means of a tool, ie. an adjustable  
spanner.  
11. If necessary, adjust the lamp by turning it slightly to bring the  
pen back to the position marked in step 8.  
12. Reassemble the optical unit.  
22  
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6. Trouble-shooting  
6. Trouble-shooting  
The UV-1 Monitor has been designed for trouble-free use. If good chromatographic practice is  
followed, very little difficulty should be experienced. Clean optical surfaces are essential if low noise  
levels are to be maintained. The following check list of the most frequent problems is meant to be a  
guide in trouble-shooting. If the checks in this section are executed and the UV-1 still does not work  
properly, consult your local GE Healthcare representative.  
Remedy  
Symptom  
Cause  
Pilot light does not light  
1. Mains cord not plugged in  
2. Fuse blown  
Check that mains cord is plugged in  
Replace fuse. If fuse blows again  
immediately consult GE Healthcare representative  
Check by plugging table lamp in  
3. No voltage at mains socket  
Pilot light on, no recorder 1.  
Optical unit not connected  
to control unit  
Check that connecting cable is  
plugged in  
response  
2.  
Control unit not connected  
to recorder  
Recorder not operating  
Check connection between output  
terminals and recorder  
Check recorder function  
Zero recorder  
3.  
4. Recorder zero not set correctly  
5. Recorder range incorrect  
6. Wrong filter  
Set recorder range to 10 mV  
Check that the filter corresponds to  
converter or aperture  
7. Filter not pushed in fully  
8. Shutter in place  
Push in filter  
Remove shutter and insert converter  
or aperture as appropriate  
Excessive noise  
1. Poor ground contact  
Check contact to ground  
2. Excess noise on mains supply  
Use alternative power source or  
remove source of disturbance  
Check connection between  
output terminals and recorder  
Set recorder on 10 mV range  
Clean cell  
3. Recorder connections  
incorrect  
4.  
5.  
6.  
Recorder range incorrect  
Dirty cell  
Deposits on optical surfaces  
Clean filter and converter  
Relocate optical unit in a clean environment  
Change to a more suitable solvent  
2 h warm-up, in cold room 12 h  
De-gas solvent. Check for leaks  
Check lamp and replace if necessary  
7.  
Solvent with high UV absorption  
8. Lamp not warmed up  
8. Bubbles passing through the cell  
Aging lamp  
9.  
Excessive baseline drift  
1. Variable absorbance gradient  
2.  
Compensate by use of reference cell  
Use fresh solvent. Check that plastic tubing  
does not leak UV absorbing substances  
Relocate optical unit or remove  
source of temperature change  
Contaminated solvent  
3. Large variation in ambient  
temperature  
4. Instrument warm-up  
Allow 2 hours warm-up  
5.  
Flush reference cell with dry gas or  
fill it with the appropriate solvent  
Condensation forming in  
empty reference  
Long term noise,  
1. Variations in ambient temperature  
especially in cold room  
2. Flow rate variations  
3. Poor ground contact  
4. Bubbles passing cell  
5. Dirty cell  
Relocate optical unit or protect  
from draught  
often regular waves in  
recorder reponse  
Check pump system and column packing  
Check contact to ground  
De-gas solvent. Check for leaks  
Clean cell  
6. Deposits on optical surfaces  
Clean filter and converter  
Relocate optical unit in a clean environment  
23  
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7. Technical Specifications  
7. Technical Specifications  
254 nm, 280 nm and 405 nm  
Wavelength range  
Lamp  
Hg lamp: for 254, 280, and 405 nm  
Life time lamp: 8000 hours  
converter: 2000 hours  
Interference. Stray light maximum 0,1 %  
at 254 nm, maximum 0.8% at 280 nm  
Filters  
AU or Transmission  
Operating modes  
Full scale ranges  
Noise  
0.01, 0.02, 0.05, 0.1, 0.2, 0.5, 1 or 2 AUFS  
4 x 10-5 AU peak to peak maximum at  
254 nm (dry cell)  
2 x 10-4 AU peak to peak (typical at  
254 nm in flowing liquid)  
5 x 10-4 AU peak to peak (typical at  
280 nm flowing liquid)  
At 254 nm, better than ± 3% to 2 AU  
At 280 nm, better than ± 5% to 1 AU  
2 x 10-4 AU/ °C typical with dry cell at  
Linearity  
Temperature drift  
254 nm  
x 10-3 AU/ °C typical with dry cell  
at 280nm  
1 x 10-4 AU/ h at 254 nm, constant  
Long term drift  
temperature after 2 hours warm-up  
4 x 10-4 AU/ h at 280 nm, constant  
temperature after 2 hours warm-up  
1.5 s to 90% of FSD at all ranges  
0-10 mV  
Time constant  
Recorder output  
Environment  
0 to +40 °C, 20-95 % relative humidity,  
84-106 kPa (840-1060 mbar) atmospheric  
pressure  
20 VA  
Power consumption  
100/ 120/ 220-230/ 240  
Power supply, voltage  
frequency  
50-60 Hz  
Control unit: 180x145x75 mm  
Optical unit: 180x145x75 mm  
Dimensions (LxWxD)  
Control unit: 1.6 kg Optical unit: 1.7 kg  
Weight  
This product meets the requirement of the EMC Directive 89/336/EEC through the harmonized standards  
EN 50081-2 (emission) and EN 50082-1 (immunity)  
EMC standards  
Note: This is a class A product. In a domestic environment this product may cause radio interference in which  
case the user may be required to take adequate actions.  
Note: The declaration of conformity is valid for the instrument when it is:  
used in laboratory locations  
used in the same state as it was delivered from GE Healthcare Bio-Sciences AB except for alteration described in the  
User Manual  
used as “stand alone” unit or connected to other CE labelled GE Healthcare products or other products as  
recommended.  
Safety standards  
This product meets the requirement of the Low Voltage Directive (LVD) 73/23/EEC through the harmonized  
standard EN 61010-1.  
24  
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8. Accessories and Spare Parts  
8. Accessories and  
Spare Parts  
Please order accessories and spare parts according to the designation  
and code numbers given below.  
Designation  
Pieces  
Code No.  
Filters  
Filter kit, 254 nm  
Filter kit, 280 nm  
Filter kit, 405 nm  
Aperture  
19-2432-01  
19-2433-01  
19-4724-01  
19-2492-01  
19-2486-01  
1
1
1
1
1
Converter 280 nm  
Flow cells  
Flow cell S-2 complete  
with measuring cell  
Flow cell 10 mm complete  
with measuring cell  
Flow cell 3 mm  
19-4840-02  
19-2504-02  
19-2503-02  
19-6254-02  
19-4510-02  
1
1
1
1
1
with measuring cell  
Flow cell HR-10  
with measuring cell  
Flow cell large volume  
with measuring cell  
Measuring cell, 3 mm  
Measuring cell, 10 mm  
19-2525-01  
19-2524-01  
1
1
Accessories and Spare parts  
UV lamp complete  
19-3807-01  
19-2505-01  
19-0041-01  
19-2491-01  
19-0379-01  
19-2853-01  
19-2447-01  
19-2448-01  
19-2925-01  
19-2926-01  
19-2367-01  
19-2368-01  
1
1
1
1
1
1
1
1
1
1
5
5
Tubing and fittings  
Tubing, PTFE, (pack of 5 m)  
Shutter  
Allen key  
Signal input cable  
Mains cable, US  
Mains cable, EU  
Fuse holder for 125 mA fuse  
Fuse holder for 250 mA fuse  
Fuse, 125 mA for 220 V  
Fuse, 250 mA for 110 V  
25  
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