Leica ES2
Leica EZ4
Leica EZ4 HD
Manual
Chapter Overview
Safety and Use
6
10
18
29
37
51
62
64
67
Leica ES2
Leica EZ4 and Leica EZ4 HD
Eyepieces (only for Leica EZ4)
Photography Using the Leica EZ4 HD
The Camera Menu
Care and Transport
Specifications
Dimensions
Leica E Series
Manual
3
Table of Contents
Leica ES2
Eyepieces (only for Leica EZ4)
Overview: Leica ES2
11
12
13
14
15
16
17
Changing Eyepieces (Only for Leica EZ4, Open Version)
Eyepieces and Eyecups (Only for Leica EZ4, Open Version)
Dioptric Correction (Only for Leica EZ4, Open Version)
Graticules (Only for Leica EZ4, Open Version)
Graticules: Preparation
30
31
32
34
35
36
Transport, Placement and Storage
The Correct Interpupillary Distance
Using the LED illuminator
Focusing
Magnification Display
Adjusting the Resistance of the Focus Drive
Inserting the Graticules
Photography Using the Leica EZ4 HD
Leica EZ4 HD: Overview of the instrument
General Instructions
Introduction
Standard delivery
Leica EZ4 and Leica EZ4 HD
Overview: Leica EZ4
38
39
40
41
42
43
45
46
47
48
49
50
19
20
21
22
23
24
25
26
27
28
Instrument variant: Leica EZ4 HD
Transport, Placement and Storage
The Correct Interpupillary Distance
Switching on the LED Illuminator
Controlling the Automatic Shutoff
Illumination types
Focusing
Magnification Display
Adjusting the Resistance of the Focus Drive
HD mode and PC mode
Capturing Images without a Computer in HD Mode
High-resolution Video Display without a Computer (HD mode)
PC Mode: Use with a Computer
PC Mode: Limitations
PC Mode: Additional Use of a HDMI Device
Configuring the White Balance
Restoring Factory Settings
Leica E Series
Manual
4
Contents (Continued)
The Camera Menu
Optional Remote Control
52
53
54
55
56
57
58
59
60
61
Viewing Images and Movies with the Optional Remote Control
Calling up the Camera Menu
COLOR (Automatic White Balance)
COLOR (Manual White Balance)
EXPOSURE
RESOLUTION
SETUP CAMERA (Camera Settings)
SETUP USER (User-defined Settings)
Predefined Illumination Scenarios
Care and Transport
Care and Transport
63
65
Specifications
Camera Module
Dimensions
Leica ES2
Leica EZ4 HD
Leica EZ4, 10× Eyepieces
Leica EZ4 without Eyepieces
68
70
72
74
Leica E Series
Manual
5
Safety and Use
Leica E Series
Manual
6
Safety Notes
Before installing, operating or using the
Warning of a danger
This symbol indicates especially impor-
EC Declaration of Conformity
instrument, it is mandatory to read this
user manual. In particular, please observe all
safety instructions.
Electrically operated accessories are construc-
ted based on the state of the art of technology
and are provided with an EC Declaration of
Conformity.
tant information that is mandatory to
read and observe. Failure to follow these inst-
ructions may pose hazards to personnel, cause
instrument malfunctions or damage the instru-
ment.
The "Safety Concept" brochure contains additi-
onal safety information regarding service work,
requirements and the handling of the micro-
scope, accessories as well as general safety inst-
ructions.
Contact address
Leica Microsystems (Switzerland) Ltd.
Industry Division
Max Schmidheiny Strasse 201
CH-9435 Heerbrugg (Switzerland)
Hazardous electrical voltage
This symbol indicates especially impor-
tant information that is mandatory to
read and observe. Failure to follow these inst-
ructions may pose hazards to personnel, cause
instrument malfunctions or damage the instru-
ment.
You can combine individual system articles with
articles from external suppliers. Please read the
user manual and the safety requirements of the
supplier.
To maintain the unit in its original condition
and to ensure safe operation, the user must
follow the instructions and warnings contained
in these user manuals.
Important information
This symbol indicates additional infor-
mation or explanations that intend to
provide clarity.
Leica E Series
Manual
7
Instructions for Use
Intended use
Interference
Handling electrical components
Never install any other plug (NEMA
5-15P) or unscrew any mechanical
components unless expressly instructed to do
so in the instructions.
The E Series microscopes are optical instru-
ments for improving the visibility of objects
through magnification and illumination. It is
used for observing and documenting.
Avoid large temperature fluctuations, direct
sunlight and vibrations. These conditions
can distort measurements and micrographic
images.
Place of use
In warm and warm-damp climatic zones,
the individual components require
special care in order to prevent the build-up of
fungus.
The microscope must be connected to a
grounded outlet.
Use
the
instrument
rooms at
in
+10
enclosed,
°C to
dust-free
+40 °C only. Protect it from oil, chemicals and
extreme humidity. It must be at least 10 cm
from the wall and away from flammable subs-
tances.
Always position the microscope so that
you can disconnect it from the power
supply at any time. The power cable is provided
as the power disconnect device.
Use in clean rooms
Leica E Series microscopes can be used in clean
rooms without any problems.
Leica E Series
Manual
8
Instructions for Use (Continued)
Intervention from the user
Maintenance
Danger of infection
Direct contact with eyepieces is a poten-
tial transmission method for bacterial
and viral infections of the eye. The risk can be
kept to a minimum by using personal eyepieces
for each individual or detachable eyecups.
The microscopes of the E Series contain
no components that can be maintained
or repaired by the user. Exceptions are menti-
oned specifically in this manual.
Leica E Series microscopes are basically
maintenance-free. To ensure that it
always operates safely and reliably, we recom-
mend that you take the precaution of contac-
ting the responsible service organization. Here
you can arrange for periodic inspections and
conclude a maintenance contract (recommen-
ded).
Unless otherwise specified in this
manual, repair and service work may
be carried out by authorized Leica technicians
only.
Unauthorized alterations to the instru-
ment or noncompliant use shall void all
rights to any warranty claims.
Accessories
Only those accessories may be used that are
listed in this user manual or for which safe use
has been confirmed by Leica Microsystems.
Leica E Series
Manual
9
Leica ES2
Leica E Series
Manual
10
Overview: Leica ES2
1
2
3
4
5
6
7
10× fixed eyepieces for eyeglass wearers
Carrying handle
Two-level magnification changer 10× and 30×
Focusing drive
Integrated LED incident light illumination
Integrated LED transmitted illumination
Switch for incident and transmitted light
1
2
3
4
5
6
7
Leica E Series
Manual
11
Transport, Placement and Storage
Built-in carrying device
All Leica E Series microscopes are equip-
ped with a carrying handle for conveni-
ent and safe transport.
Setting up the microscope
1. Place the microscope on a flat tabletop.
Packing the device away
1. After use, wind up the cable as shown
in the illustration.
2. Connect the power supply to a grounded
socket.
2. Store the instrument so that it is protected
against dust.
Leica E Series
Manual
12
The Correct Interpupillary Distance
The Ideal Image
The interpupillary distance is correctly
set if you see a single circular image field
when looking at a specimen.
Note for eyeglass wearers
Setting the eye distance
1. Look into the eyepieces.
If you wear glasses, fold the eyecups back
– otherwise, fold the eyecups forwards.
2. Hold the eyepieces with both hands. Push
the eyepieces together or separate them
until you see a circular image.
✓
✗
You may need a short time to become familiar
with using the instrument. Not to worry—after
a little while, it will become automatic.
Leica E Series
Manual
13
Using the LED illuminator
Iluminating the specimen
1. Position a specimen in the middle of the
baseplate.
Tips on use
Ϙ
Ϙ
Ϙ
Use incident light for plastic, opaque
objects.
2. Switch the two LED illuminators on or off as
desired.
Use transmitted light for transmitted light
specimens or transparent objects.
Use the combination of both LED illumina-
tors for partially transparent objects.
After switching off, the LEDs remain lit for
about another 4 seconds.
Leica E Series
Manual
14
Focusing
Focusing raises and lowers the stereomi-
Focusing
3. Set the magnification changer to the
second level (30).
croscope using the focusing drive. The
specimen is brought into sharp focus as soon as
it is in the focal point of the objective.
1. Set the magnification changer to the lowest
level (10).
4. Use the focusing drive once again for fine
focus.
The focusing drive can be operated either left-
or right-handed.
After you have adjusted the image sharp-
ness at high magnification, it is maintai-
ned even if you switch to a lower magnification
(parfocal).
2. Set the working distance to approx.
100 mm for coarse focusing.
Focusing drive torque
Leica E Series
Manual
15
Magnification Display
You can read the configured magnifica-
tion on the right-hand rotary knob of the
Leica ES2. The additional magnification provi-
ded by the eyepieces is already incorporated in
the scale.
Leica E Series
Manual
16
Adjusting the Resistance of the Focus Drive
Adjusting the resistance
Is the focusing drive too easy or too difficult
to adjust? No problem - the resistance can be
adjusted individually depending on your perso-
nal preferences:
1. Grip the drive knobs with both hands and
turn them towards each other until the
desired resistance is reached during focu-
sing.
Leica E Series
Manual
17
Leica EZ4 and Leica EZ4 HD
Leica E Series
Manual
18
Overview: Leica EZ4
1
2
3
4
5
6
7
10x fixed eyepieces for eyeglass wearers
Carrying handle
Magnification changer
Focusing drive
Integrated LED incident light illumination
Illumination control
1
2
3
Integrated LED transmitted illumination
4
5
6
7
Leica E Series
Manual
19
Instrument variant: Leica EZ4 HD
1
2
1
2
3
4
5
6
7
8
9
10x fixed eyepieces for eyeglass wearers
Integrated digital camera with SD card slot
Carrying handle
Magnification changer
IR receiver for optional remote control
Focusing drive
Integrated LED incident light illumination
Illumination control
Integrated LED transmitted illumination
3
4
5
6
7
8
9
Leica E Series
Manual
20
Transport, Placement and Storage
Built-in carrying device
All Leica E Series microscopes are equip-
ped with a holder for convenient and
safe transport.
Setting up the microscope
1. Place the microscope on a flat tabletop.
Packing the device away
1. After use, wind up the cable as shown
in the illustration.
2. Connect the power supply to a grounded
socket.
2. Store the instrument so that it is protected
against dust.
Leica E Series
Manual
21
The Correct Interpupillary Distance
The Ideal Image
The interpupillary distance is correctly
set if you see a single circular image field
when looking at a specimen.
Note for eyeglass wearers
Setting the eye distance
1. Look into the eyepieces.
If you wear glasses, fold the eyecups back
– otherwise, fold the eyecups forwards.
2. Hold the eyepieces with both hands. Push
the eyepieces together or separate them
until you see a circular image.
✓
✗
You may need a short time to become familiar
with using the instrument. Not to worry—after
a little while, it will become automatic.
Leica E Series
Manual
22
Switching on the LED Illuminator
Automatic shutoff
If the illumination is not operated for 60
2. Switch the two LED illuminators on or off as
For a detailed description of the illumina-
desired:
minutes, it switches off automatically.
The illumination can be reactivated by pressing
a button on the membrane keypad.
LED illumination
1. Position a specimen in the middle of the
baseplate.
Ϙ
Ϙ
Ϙ
Use incident light for plastic, opaque
objects
Use transmitted light for transmitted light
specimens or transparent objects.
Use the combination of both LED illumina-
tors for partially transparent objects.
Leica E Series
Manual
23
Controlling the Automatic Shutoff
Automatic shutoff
The factory settings of the EZ4 models
switch the instrument off automatically
after one hour without user interaction. This
automatic shutoff helps to conserve the light-
emitting diodes and save energy.
Disabling the automatic shutoff
To disable the automatic shutoff, follow these
steps:
Enabling the automatic shutoff
To re-enable the automatic shutoff, follow
these steps:
1. Switch the microscope off.
1. Switch the microscope off.
2. Press and hold the
and
buttons on
2. Press and hold the
and
buttons on
the light control unit simultaneously while
you switch on the microscope.
the light control unit simultaneously while
you switch on the microscope.
The LEDs flash twice slowly and then remain
switched on. The automatic shutoff is now disa-
bled.
The LEDs flash three times slowly and then
remain switched on. The automatic shutoff is
now re-enabled.
Leica E Series
Manual
24
Illumination types
Three-level incident light illumination
The incident light illumination is made
up of 5 LEDs. Two, three or five diodes are
switched on depending on the level. Accord-
ingly, the angle of incidence of the light beam
on the object is switched from high to low.
Regulating brightness
The brightness is adjusted using the and
keys and affects all switched-on LEDs.
Press 1×
Press 2×
Press 3×
Press 4×
Incident light off
Press 1×
Five diodes: greatest
brightness for incident
light objects
Top three diodes: shadow-
less uniform lighting for
objects with highly uneven
surfaces
Two diodes: side light
emphasizes structures and
increases the contrast
Switch on transmitted light
for transparent objects
and transmitted light
specimens.
Leica E Series
Manual
25
Focusing
Focusing raises and lowers the stereomi-
Focusing
3. Set the magnification changer to the
second level (35).
croscope using the focusing drive. The
specimen is brought into sharp focus as soon as
it is in the focal point of the objective.
1. Set the magnification changer to the lowest
level (8).
4. Use the focusing drive once again for fine
focus.
The focusing drive can be operated either left-
or right-handed.
After you have adjusted the image sharp-
ness at high magnification, it remains
constant even if you switch magnification levels
(parfocal).
2. Set the working distance to approx.
100 mm for coarse focusing.
Focusing drive torque
Leica E Series
Manual
26
Magnification Display
You can read the configured magnifica-
tion on the right-hand rotary knob of the
Leica EZ4 models. The additional magnification
provided by the eyepieces is already incorpora-
ted in the scale
Leica EZ4 models with open tubes
On the EZ4 model without eyepieces,
only the magnification factors of the
magnification changer are indicated: 0.8, 1,
1.25, 1.6, 2, 2.5, 3, 3.5.
Different total magnifications are possi-
ble depending on which eyepieces are
used with the EZ4. The magnification of the
eyepieces is indicated on their outer surfaces,
e.g. 10×/20, 16×/16 or 20×/12.
Leica E Series
Manual
27
Adjusting the Resistance of the Focus Drive
Adjusting the resistance
Is the focusing drive too easy or too difficult
to adjust? No problem - the resistance can be
adjusted individually depending on your perso-
nal preferences:
1. Grip the drive knobs with both hands and
turn them towards each other until the
desired resistance is reached during focu-
sing.
Leica E Series
Manual
28
Eyepieces (only for
Leica EZ4)
Leica E Series
Manual
29
Changing Eyepieces (Only for Leica EZ4, Open Version)
Changing eyepieces
Various eyepieces are available for the Leica
EZ4, allowing the stereomicroscope to be adap-
ted even better to the specific requirements.
The following eyepieces are available for selec-
tion:
Ϙ
Eyepieces replaceable, fixed or adjustable:
10×/20, 16×/16 or 20×/12
Ϙ
Eyepieces for eyeglass wearers 10×/20B
and 16×/15B, fixed and adjustable
Adjustable eyepieces for eyeglasses with straight
eyecups.
The interchangeable eyepieces also offer the
following advantages:
Ϙ
The dioptric range is adjustable from +5 to
–5
Ϙ
Measurement graticules can be used, see
page 34 and the following.
Leica E Series
Manual
30
Eyepieces and Eyecups (Only for Leica EZ4, Open Version)
Eyecups
The eyecups can be replaced.
Risk of infection
Direct contact with eyepieces is a poten-
tial transmission method for bacterial
and viral infections of the eye. The risk can be
minimized by the use of individual eyecups.
Eyeglass eyepiece with
adjustable diopter and straight
eyecups
Eyepiece for users who do not
wear eyeglasses with adjustable
diopter and inclined eyecup
Fixed eyepiece with inclined
eyecup.
Leica E Series
Manual
31
Dioptric Correction (Only for Leica EZ4, Open Version)
Dioptric Correction
The adjustable eyepieces for the Leica
Users with normal vision do not need adjus-
table eyepieces. An adjustable eyepiece is
sufficient for mild shortsightedness.
Adjusting
1. Set the dioptric correction for both eyepie-
ces to "0".
EZ4 are also available with built-in diop-
tric correction, allowing the microscope to be
used without glasses even by those with vision
problems. The correction comprises a maxi-
mum of 5 diopter settings.
If vision is extremely poor or when graticules
are used, two adjustable eyepieces are needed.
2. Select the lowest magnification and focus
on a flat specimen.
When the diopters are regulated exactly, the
sharpness is constant over the entire magnifi-
cation range (=parfocal).
3. Select the highest magnification and read-
just the sharpness.
4. Select the lowest magnification again, but
do not look into the eyepieces.
Once the diopters have been set, the procedure
does not have to be repeated. Simply restore
your diopter values on the eyepiece before
beginning work with the stereomicroscope
5. Carry out steps 6 to 8 for each objective
individually.
6. Rotate the eyepiece counterclockwise in
the "+" direction as far as it will go (+5 diop-
ter settings).
Leica E Series
Manual
32
Dioptric Correction (Continued)
7. Look into the eyepiece.
8. Slowly rotate the eyepiece individually
in the "–" direction until the eye sees the
object sharply imaged.
9. Select the highest magnification and refo-
cus if necessary.
Now, if you adjust the magnification from the
highest to the lowest level, the specimen is
always brought into sharp focus. If not, repeat
the process.
Leica E Series
Manual
33
Graticules (Only for Leica EZ4, Open Version)
Graticules
Available eyepiece inserts
The transparent graticules can be inser-
ted in the adjustable eyepieces of the
Graticules
10 mm/ 0.1 mm
5 mm/ 0.1 mm
5 mm/ 0.05 mm
100 Div / 0.002"
Leica EZ4. They enable easy measurements at
the specimen.
100 Div / 0.001"
For equipment with a graticule in one
eyepiece, two adjustable eyepieces
are needed. Since one eye is focussed on the
eyepiece with the graticule, it needs a second,
adjustable eyepiece so that it can be aligned
optically with the first.
150 Div / 0.0005"
50 mm (0.1 / 0.01 mm graduation)
1" (0.001" graduation)
Crosshairs
stage micrometers
Crosshairs
Leica E Series
Manual
34
Graticules: Preparation
Care instructions
Variant 1: 10× and 20× eyepieces
Variant 2: 16× eyepieces
The graticule must always be clean and
dust-free! Never touch the graticule with
bare hands! Always hold the graticule by the
edges!
The 10× and 20× adjustable eyepieces include
an insert at the bottom with a snap mount.
On the 16× adjustable eyepieces, the insert is
fixed to the eyepiece by a screwed connection.
1. Gently press the two small tabs on the snap
mount together and pull out the insert.
1. Screw the insert out counterclockwise.
Leica E Series
Manual
35
Inserting the Graticules
Inserting the graticules is the same for all eyepi-
ece types.
4. Put the insert back into the eyepiece.
Depending on the variant, you have to
either press or twist the insert into place.
6. Align the graticules by turning the eyepiece
in the tube.
1. Place the insert with the knurled side on
the work surface.
2. Grasp the graticule so that you see the scale
in laterally correct form.
3. Carefully take hold of the edge of the grati-
cule and push it sideways into the holder
in the insert. Make sure that both plastic
holders snap into place.
5. Insert the eyepiece into the tube.
Leica E Series
Manual
36
Photography
Using the Leica EZ4 HD
Leica E Series
Manual
37
Leica EZ4 HD: Overview of the instrument
1
1
3
2
3
2
4
1
2
3
4
SD card slot
Status LED
Black multifunction button
Red multifunction button
1
2
3
USB connection
Pinhole switch
HDMI connection
Leica E Series
Manual
38
General Instructions
General Instructions
Ϙ
Ϙ
Ϙ
The software for control is included on the
DVD provided with the instrument.
Install the software before connecting the
Leica EZ4 HD to the PC or Mac.
As soon as the EZ4 HD is connected to a
power cable and the main switch is turned
on, the integrated camera is also ready for
use.
Ϙ
If a USB cable is not connected and there is
no SD card in the card slot of the Leica EZ4
HD, the status LED of the instrument lights
up RED.
Leica E Series
Manual
39
Introduction
The Leica EZ4 HD puts some extremely interes-
ting additional functions at your fingertips. The
integrated digital camera is designed for the
sole purpose of giving you the best possible
quality for your documentation – with particu-
larly easy handling.
Leica Application Suite EZ
Together with the Leica EZ4 HD, you have also
obtained the "Leica Application Suite EZ" soft-
ware (standard LAS on request). It allows vari-
ous tasks to be completed in which the televi-
sion and monitor can even be used together.
Thus you are ideally prepared for every situa-
tion: work at the computer, presentations, trai-
ning sessions, trade show appearances, mate-
rial testing and more.
Real Full HD display
The integrated HDMI output allows the micro-
scope image to be output to a High-Definition
(HD)-capable display unit (monitor, television
set or projector), where the following resolu-
tions are possible:
User-friendly even in the smallest detail
Like every digital camera, that of the Leica EZ4
HD reacts differently to different light sources.
However, the white balance is factory-set to
match the LED illumination of the microscope.
This ensures that you obtain the best results
automatically.
Ϙ
1920×1080i ("Full HD"). This resolution
provides the best possible imaging perfor-
mance on a Full HD-capable display unit.
Ϙ
1280×720p ("HD ready"). This resolution is
suitable for display on televisions labeled
"HD ready".
Leica E Series
Manual
40
Standard delivery
DVD with Leica software,
drivers and manuals
USB cable
HDMI cable
SD card
Optional: Remote control
with battery (Type CR2025)
Please do not discard any of the packing
materials. They should be used for safely
storing and transporting the instrument should
the need arise.
Leica E Series
Manual
41
HD mode and PC mode
The camera is normally in what is known as "HD
mode" after being switched on. In this mode, it
outputs a high-resolution signal in 16:9 wide-
screen format. The images can then be saved
directly to an SD card.
If the camera is in PC mode, images
cannot be captured directly to an SD card.
All images are stored on the PC.
Optional remote control
You can use the optional remote control
only if the camera is HD mode – with the
exception of the "i" button, which you can use
to show various information on the monitor
even in PC mode.
If the camera is in HD mode, it is not
detected by the Leica software. All
images are stored on the SD card.
If the Leica EZ4 HD is connected to a computer
via USB and the Leica software provided is star-
ted, the camera switches automatically to what
is called "PC mode". In this mode, images are
transmitted to the computer with a size of up
to 3 megapixels. The aspect ratio is always 4:3.
The microscope remains in the last selec-
ted mode (HD or PC mode) until you
change it. You can do this automatically by star-
ting the Leica software or manually by pressing
and holding the black multifunction button for
5 seconds.
Leica E Series
Manual
42
Capturing Images without a Computer in HD Mode
Saving to SD card
Images can be saved directly to an inser-
ted SD card. The card has to be formatted
using the "FAT" file system.
Changing between PC and HD mode
Detecting the active mode
In order to take photos without a compu-
ter you must be in HD mode (not PC
mode). After the camera is switched on, it is in
the last selected mode. However, it switches to
PC mode whenever the Leica software is started.
Ϙ
The camera is in HD mode when the LED
status display is illuminated in red.
Ϙ
The camera is in PC mode when the LED
status display is illuminated in red and
flashes green approximately once per
second.
When the SD memory card is full, the LED
on the camera is illuminated in red. No
more images can be captured.
1. To switch between PC and HD mode, press
and hold the black multifunction button for
5 seconds.
Leica E Series
Manual
43
Capturing Images without a Computer in HD Mode (Continued)
Capturing an image
1. Insert the SD memory card into the SD card
slot until you hear it click into position.
2. Press the red image capture button briefly.
You hear an audible signal as confirmation
and the camera LED flashes green.
The LED illuminates in green. The camera is now
ready to save images to the SD card.
You will hear another beep when the Leica
ICC50 HD has completed capturing the image.
The image is now saved on the SD card.
Leica E Series
Manual
44
High-resolution Video Display without a Computer (HD mode)
Cables
The HDMI cable required for this purpose
is included in the standard delivery of the
Leica EZ4 HD.
Changing the Resolution
The output resolution (Full HD or HD ready) is
configured directly on the microscope.
1. Connect the camera and the HDMI device
via the integrated HDMI port.
1. Press a pin into the pinhole switch in order
to change the resolution.
Live images and captured images can be
output in high resolution via the HDMI
cable:
Ϙ
Ϙ
Ϙ
Full HD: 1920×1080 pixels
HD ready: 1280×720 pixels
Movie: 720×480 pixels
Leica E Series
Manual
45
PC Mode: Use with a Computer
For instructions on operating the instru-
ment in PC mode, refer to the DVD inclu-
3. Follow the instructions in the software for
adjusting and acquiring an image.
ded in the standard delivery of the Leica EZ4 HD.
The 10× eyepieces of the Leica EZ4 HD
have a field of view of 20 mm. Unlike the
circular object field through the eyepieces, the
image section of the camera is rectangular and
has a diagonal measurement of 16.4 mm. There-
fore, the image field of the camera is smaller
than the visible object field through the eyepi-
eces.
The LED changes color from yellow to
red. You can now start the software. Once
you have done so, the LED flashes yellow briefly,
then stays steady green.
Installing and Connecting
1. Place the DVD provided into the computer's
disk drive and follow the instructions for
loading the software.
2. Connect the camera and the computer
using the USB cable.
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46
PC Mode: Limitations
Aspect ratio
If the Leica EZ4 HD is connected to a
computer and is in PC mode, images are
always output in a 4:3 size ratio and in a lower
resolution – even if a 16:9 monitor or television
set is connected via HDMI. The image is neces-
sarily compressed on such a device.
In this case, we recommend that you not
connect the television to the microscope, but
to the computer as a second monitor.
4:3 aspect ratio
16:9 aspect ratio
Remote control
If the Leica EZ4 HD is in PC mode, the
optional remote control cannot be used.
The menus of the camera are also no longer
displayed on the monitor. Instead, all settings
are configured on the computer using the Leica
software.
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47
PC Mode: Additional Use of a HDMI Device
In PC mode, the camera image is always
shown in the preview window of the
Leica software. In addition, another computer
monitor or television can be connected via the
HDMI interface.
To use a higher resolution on the connec-
ted screen, select HD mode on the
camera:
Connecting the HDMI monitor
1. Connect the HDMI cable provided to the
camera.
1. To switch between PC and HD mode, press
and hold the black multifunction button for
5 seconds.
The image resolution on the attached
HDMI or television screen is limited to
the live image resolution inside the LAS EZ
software. Due to technical constraints, it is not
possible to send a live image stream to the
connected screen in "HD ready" or "Full HD"
quality.
2. Connect the HDMI cable to the HDMI televi-
sion or monitor.
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48
Configuring the White Balance
When configuring the white balance of
the camera, make sure that no specimen
is in the beam path and that a standard illumi-
nation is available.
e recommend carrying out a new
Wwhite balance adjustment whene-
ver you change the illumination type or color
temperature. This is particularly necessary if
you are working with an external light source
that can be switched from yellow (low inten-
sity) to blue (high intensity). The new white
balance generates a neutral background for
both types of illuminator.
5 sec.
Configuring a New White Balance
Press and hold the red exposure button for
5 seconds. Wait for the beep. As soon as the
signal sounds, a new white balance has been
configured.
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Restoring Factory Settings
Factory settings
1. Press the red multifunction button for 10
seconds to delete all individual settings
and reset the instrument to the factory
settings.
10 sec.
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The Camera Menu
If you purchased the optional remote, you
will have access to the camera menu.
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Optional Remote Control
The remote control does not work if the
camera is in PC mode.
1. Start video recording
2. Stop video recording
3. Save still image to SD card
4. Stop/continue live image
Pause/play movie clip
1
3
5
2
4
6
5. Playback mode
6. Show / hide information box
7. Arrow keys for navigation
8. OK / confirm
9. Call up the camera menu
7
8
9
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Viewing Images and Movies with the Optional Remote Control
If you have purchased the optional
remote control, you can display the
images and videos captured by the HD camera
directly on a HD screen.
3. To return to live view press any button on
the camera or press the key 1, 2 or 9 on the
remote control (see previous page).
007/0109 ICC500007.MP4
1. Miniature view: (press 1×)
Ϙ
Ϙ
Select miniature:
,
,
and
Show full-size image or play back video:
Playback / fullscreen view
007/0109 ICC500007.MP4
2. Play back individual image: (press 2×)
Ϙ
Ϙ
Ϙ
Previous/next image: or
Play back video:
Fast forward/reverse: or
Thumbnail view
Leica E Series
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53
Calling up the Camera Menu
The remote control does not work if the
camera is connected to a computer and
is in PC mode. The menus of the camera are
also no longer displayed on the monitor. In this
case, press the black multifunction button for
5 seconds to switch to HD mode (refer also to
page 42).
Opening and closing the camera menu
1. Point the remote control towards the
microscope.
2. Press the ▤ key on the remote control to
show the camera menus on the monitor.
3. Press the ▲▼◀ and ▶ buttons to select a
menu item.
4. Press the "OK" button to confirm a menu
item.
5 sec.
5. Press the ▤ button again to hide the
camera menus.
Leica E Series
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COLOR (Automatic White Balance)
Enabling automatic white balance
COLOR
SET WHITE BAL
WB MODE
PRESS OK
AUTO
1. Press the ▤ button on the remote control.
EXPOSURE
RESOLUTION
SETUP CAMERA
SETUP USER
2. Call up the "COLOR" entry.
RED LEVEL
BLUE LEVEL
BLACK LEVEL
3. Set the value for "WB MODE" to "AUTO".
4. Press the ▤ button to exit the menu.
MAIN MENU
SUBMENU
The "COLOR" function makes it possible to adapt the camera to the
ambient light so that color-neutral images can be acquired.
The values for "RED" and "BLUE" cannot be adjusted if the "WB
MODE" setting is set to "AUTO".
If the "WB MODE" is set to "AUTO", the white balance is carried out
continuously. This mode is recommended only for color-neutral
specimens and those stained over a wide area.
Leica E Series
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55
COLOR (Manual White Balance)
Adjusting the white balance manually (recommended)
1. Move the specimen out of the field of view so that only the illumina-
tion is seen.
COLOR
SET WHITE BAL
WB MODE
PRESS OK
MANUAL
EXPOSURE
RESOLUTION
SETUP CAMERA
SETUP USER
RED LEVEL
2. Press the ▤ button on the remote control.
3. Call up the "COLOR" entry.
BLUE LEVEL
BLACK LEVEL
MAIN MENU
SUBMENU
4. Select the "SET WHITE BAL" menu item and press the "OK" button.
4. If necessary, correct the values for "RED" and "BLUE" until the image
shows a uniform gray area.
The "COLOR" function makes it possible to adapt the camera chip
to the ambient light so that color-neutral images can be acquired.
5. Press the ▤ button to exit the menu.
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EXPOSURE
Manual exposure
1. Press the ▤ button on the remote control.
MANUAL
COLOR
EXP MODE
EXPOSURE
GAIN
EXPOSURE
RESOLUTION
SETUP CAMERA
SETUP USER
2. Call up the command for manual exposure (see diagram at the left).
3. Set the value for "EXP MODE" to "MANUAL".
BRIGHTNESS
GAMMA
4. Correct the values for "EXPOSURE", "GAIN" and "GAMMA" until you
obtain the desired results.
MAIN MENU
SUBMENU
Automatic exposure
1. Press the ▤ button on the remote control.
AUTO
COLOR
EXP MODE
EXPOSURE
GAIN
EXPOSURE
RESOLUTION
SETUP CAMERA
SETUP USER
2. Call up the command for automatic exposure (see diagram at the left).
3. Set the value for "EXP MODE" to "AUTO".
BRIGHTNESS
GAMMA
4. Correct the values for "BRIGHTNESS" and "GAMMA" until you obtain
the desired results.
Main menu
Submenu
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RESOLUTION
CAPTURED (resolution of the captured image)
COLOR
LIVE
1280X1080
2048X1536
720X480
HIGH
Defines the resolution of the captured image if the Leica EZ4 HD is
not connected to a computer. The following resolutions are available:
640×480, 1024×768, 1600×1200 and 2048×1536.
EXPOSURE
RESOLUTION
SETUP CAMERA
SETUP USER
CAPTURED
MOVIE
QUALITY
SHARPNESS
MOVIE (resolution of videos)
HIGH
Defines the resolution of the recorded videos if the Leica EZ4 HD is not
connected to a computer. The resolution is currently limited to 720×480
pixels and cannot be changed.
MAIN MENU
SUBMENU
QUALITY (of the image capture)
A higher image quality can slow down the image capture process. Two
quality levels are available for selection: "NORMAL" and "HIGH".
The "RESOLUTION" menu enables you to define the resolution
for the live image on the computer, still image capture and video
capture individually. This provides optimum results in every situation.
SHARPNESS (internal camera sharpness)
Define the amount of sharpening to be applied to the image.
LIVE (resolution of the live image)
Defines the resolution of the live image. The following resolutions are
available:
Ϙ
For direct connection of the Leica EZ4 HD to a monitor or TV set:
1280×720 or 1920×1080 (16:9 aspect ratio).
Ϙ
For connection to a computer: 640×480, 1024×768 and 1440×1080
(4:3 aspect ratio)
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SETUP CAMERA (Camera Settings)
RESET CAMERA
COLOR
SET DATE TIME
RESET CAMERA
FLIP IMAGE
OVERLAY
PRESS OK
PRESS OK
0°
This command resets the camera to the factory settings. All user informa-
tion (such as white balance, resolution etc.) is reset.
EXPOSURE
RESOLUTION
SETUP CAMERA
SETUP USER
FLIP IMAGE
Rotates the live image by 180°.
NONE
CAM MODE
PC-MODE
Overlay
Main menu
Submenu
Shows the following on the HD monitor, superimposed over the live
image: Leica logo, histogram, date/time and image counter.
The camera settings enable you to control internal parameters such
as the date or time or to reset the camera. You can also switch the
camera from HD mode to PC mode (or vice versa).
CAMERA MODE
This allows you to toggle the camera between PC and HD mode. You can
also toggle between PC and HD mode by pressing the black button for
more than 5 sec until a message appears on the screen.
SET DATE TIME
This command sets the date and time as well as the format for display-
ing them. The following formats are available for selection: YYMMDD –
DDMMYY – MMDDYY.
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59
SETUP USER (User-defined Settings)
SET MENUCOLOR
COLOR
SET ILLUM
ILLUM-1
LEICA/LAS
15 SEC
Currently, two color schemes are available for the menu guidance. Additi-
onal colors may be provided in the future via a firmware update.
EXPOSURE
RESOLUTION
SETUP CAMERA
SETUP USER
MENUCOLOR
SHOW MENU
SHOW CAPTURE
SHOW CAPTURE 2 SEC
Defines how long an image is displayed on the screen after being captu-
red.
LANGUAGE
ENGLISH
Main menu
Submenu
SHOW MENU
Defines how long the menu is displayed on the screen.
The user-defined settings enable you to, for example, save illumina-
tion scenarios and retrieve them later.
SET LANGUAGE
Select the language of the menu.
SET ILLUM -1 to 3 (save illumination modes)
These settings allow you to store up to three different illumination modes
and retrieve them later. Select the number of the illumination mode and
press "OK" to store the current camera settings for later acquisition situ-
ations.
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Predefined Illumination Scenarios
The camera in the Leica EZ4 HD provides
various illumination modes that cover a
wide variety of possible situations.
2. Press the button again to switch to the next
illumination mode.
LAST USED
AUTO
3. Press the red image capture button or wait
until the menu no longer appears.
Changing the illumination mode
1. Press the black multifunction button briefly
to display the current illumination mode on
the monitor or TV set.
BRIGHTFIELD
DARKFIELD
To return to the last selected mode, press
the black multifunction button seve-
ral times in succession until the "LAST USED"
setting appears.
ILLUMINATION-1
ILLUMINATION-2
ILLUMINATION-3
If you do not press the black multifunc-
tion button for 5 seconds, the selected
illumination mode is taken over.
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Care and Transport
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Care and Transport
General Instructions
Ϙ
Avoid excessive use of solvents. The lint-
free cloth, lens cloth or cotton swab should
be soaked with solvent, but not so wet that
solvent runs over the lens.
Transport
Always use both hands to carry the
microscope. There is a handle on the
back of the microscope and an undercut in the
front for this purpose.
Ϙ
Keeping all optical components clean is
important for maintaining good optical
performance.
Ϙ
Always protect the microscope and camera
with the plastic cover when the instru-
ment is not in use. (Included in the delivery
package of the microscope.) Be sure power
to the camera is off when the dust cover is
in place.
Ϙ
Protect the microscope from moisture,
fumes and acids and from alkaline, caustic
and corrosive materials. Do not store
chemicals in the vicinity.
Ϙ
Ϙ
Protect the microscope from oil and grease.
Never grease or oil mechanical parts or
sliding surfaces.
Ϙ
If any optical surface becomes badly coated
with dust or dirt, it should be cleaned by
blowing the surface with a syringe or brus-
hing it off with a camel hair brush before
attempting to wipe the surface clean.
It is advisable to enter a service agreement
with Leica Service.
Ϙ
Optical surfaces should be cleaned using
a lint-free cloth, lens cloth or cotton swab
moistened with a commercially available
glass cleaner. Do not use alcohol.
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Specifications
Leica E Series
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Camera Module
Camera
Weight
700 g (camera only)
Height
50mm
Exposure time
Live image
2 msec – 2 sec
Max. 45 fps (1256 × 720 px) – 20 fps (1920 ×1080 px)
Full frame image acquisition 2048 × 1536 pixels, 3.1 megapixels
Movie clip
720 × 480 pixels (MP4)
Sensor size
6.55 mm × 4.92 mm
Pixel size
3.2 μm × 3.2 μm
Sensor grade
Gain
Micron 1/2“ CMOS
1× to 20×
Color Depth
24-bit
Data format
JPEG / TIFF / BMP / MP4
Operating systems
Software available
Min. computer config.
Min. display specification
Windows XP, Windows Vista, Windows 7, Mac OS X
LAS EZ software (PC), optional LAS modules, Acquire (Mac)
Intel Pentium 4 or DuoCore, 2 GHz, 2 GB RAM, 24-bit graphics, 1248 × 1024, DVD
• HD ready (1280×720)
• Full HD (1920×1080)
• Standard HDMI port*
* Connection via DVI using a HDMI–DVI adapter is not recommended!
Leica E Series
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Camera Module (Continued)
Mechanical and optical interfaces
Other
Ϙ
Ϙ
Ϙ
Ϙ
Ϙ
Integrated camera module
Ϙ
Ϙ
Ϙ
Ϙ
Ϙ
Ϙ
Ϙ
Power supply: integrated power supply
Light distribution Optical 50% / Camera 50%
Internal video magnification 0.5×
Power requirement: 4 W
Operating temperature range +5 °C to 50 °C
Relative humidity 10% to 90%
Sensor Centered to optical axis 0.1 mm
Hoizontally / Vertically aligned 1 degree
CE Declaration of Conformity Available
Tested standards EMI/RFI: EN55011-B EN50082-1
EMC: 61000-3-2 61000-3-3
Electronic interfaces
Ϙ
Ϙ
Ϙ
Ϙ
Ϙ
Ϙ
USB 2.0, mini-USB plug
Mini-HDMI (HD ready and Full HD)
LED with 2 colors: green ("ready"), red ("image capture")
Integrated slot SD (Secure Digital) card
SD card: 1-8 GB, WIFI, WORM support, High Capacity (HC)
Black multifunction button for changing the illumination mode (press
for 1 second) and for switching between HD and PC mode (press and
hold for 5 seconds)
Ϙ
Red multifunction button for image capture using SD card (press
for 1 second), configuring a new white balance (press and hold for 5
seconds), reset camera (press and hold for 10 seconds)
Leica E Series
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Dimensions
Leica E Series
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Leica ES2
Leica E Series
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Leica ES2 (Continued)
Leica E Series
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Leica EZ4 HD
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Leica EZ4 HD (Continued)
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Leica EZ4, 10× Eyepieces
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Leica EZ4, 10× Eyepieces (Continued)
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Leica EZ4 without Eyepieces
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Leica EZ4, without Eyepieces (Continued)
Leica E Series
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