Leica Projection Television CM3050 S User Manual

Leica CM3050 S

Cryostat

Instruction Manual

Leica CM3050 S – Cryostat

English V1.2– 11/2006

Always keep this manual near the instrument.

Read carefully prior to operating the instrument.

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Important note

Serial No.: .............. ......................................................

Year of Manufacture: ...............................................

Country of Origin: Federal Republic of Germany

The information contained in this document

represents state-of-the-art technology as well as

the current state of knowledge.

Leica will not assume liability for errors that might

be contained in this manual, nor for accidental

damage or damage arising from the delivery,

performance or use of this manual.

Therefore, no claims can be made based on the

text and illustrations in this instruction manual.

Leica Microsystems Nussloch GmbH reserves the

right to change technical specifications

without notice since each of our products is

subject to a policy of continuous improvement.

This document is protected under copyright laws.

Leica Microsystems Nussloch GmbH

retains all rights related to this documentation.

Any reproduction of text and illustrations - or any

parts thereof - in form of printing, photocopying,

microfiches, or other methods, including

electronic systems, requires the prior written

permission of Leica Microsystems Nussloch

GmbH.

The serial number and the year of manufacture

are specified on the nameplate at the back of the

instrument.

Issued by:

Leica Microsystems Nussloch GmbH

Heidelberger Str. 17 - 19

D-69226 Nussloch

Germany

Phone: +49 6224 143-0

Fax: +49 6224 143-200

Internet: http://www.histo-solutions.com

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Table of Contents

Important information................................................................................................................ 7

1.1

1.2

Symbols used in this manual and .................................................................................................... 7

Designated use ................................................................................................................................... 8

1.3 Qualification of personnel .......................................................................................................... 8

Sicherheit .................................................................................................................................... 9

2.1

2.2

General information on instrument design and safe handling .................................................... 9

Integrated safety devices ................................................................................................................. 9

2.2.1 Locking the handwheel ................................................................................................................... 10

2.2.2 Centering the handwheel grip ........................................................................................................ 10

2.2.3 Emergency stop function (instruments with sectioningmotor only) ........................................ 11

2.2.4 Knife guard ........................................................................................................................................ 11

2.3

Safety instructions: handling and operation of the instrument................................................. 12

2.3.1 Transport ........................................................................................................................................... 12

2.3.2 Site requirements ............................................................................................................................. 12

2.3.3 Electrical connections ..................................................................................................................... 12

2.3.4 Handling microtome knives/blades ............................................................................................... 13

2.3.5 Knife guard/handwheel lock........................................................................................................... 13

2.3.6 Motorized sectioning ....................................................................................................................... 13

2.3.7 Defrosting/Handling frozen tissue ................................................................................................. 13

2.3.8 Frozen parts of the instrument and frozen accessories ............................................................ 13

2.3.9 Infectious/radioactive material ...................................................................................................... 14

2.3.10 Disinfection and cleaning ............................................................................................................... 14

2.3.11 Removing/reinstalling the microtome ........................................................................................... 15

2.3.12 Display message ‘Dry microtome’ ................................................................................................. 15

2.3.13 Maintenance .............................................................................................................................. 15

Installation................................................................................................................................. 16

3.1

Site requirements ............................................................................................................................. 16

3.1.1 General site requirements .............................................................................................................. 16

3.1.2 Electrical connections ..................................................................................................................... 16

3.2 Unpacking and installation ............................................................................................................. 16

3.2.1 Repacking .......................................................................................................................................... 16

3.3

3.4

Standard delivery ............................................................................................................................. 17

Installing/inserting accessories..................................................................................................... 19

3.4.1 Installing the handwheel ................................................................................................................. 19

3.4.2 Inserting the accessories ............................................................................................................... 19

3.4.3 The footswitch .................................................................................................................................. 20

3.5 Prior to switching on the instrument ........................................................................................ 20

Instruction Manual V1.2 - 11/2006

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Table of Contents

Instrument Properties .............................................................................................................. 21

4.1 Overview ............................................................................................................................................ 21

4.2 Technical Data ........................................................................................................................... 23

Operation ................................................................................................................................... 25

5.1

5.2

5.3

Operating the instrument for the first time ................................................................................... 25

Switching on/fuses........................................................................................................................... 26

Control panel 1 .................................................................................................................................. 27

5.3.1 Key functions in control panel 1..................................................................................................... 27

5.3.2 Display functions in control panel 1 .............................................................................................. 27

5.3.3 Display indications when switching on ........................................................................................ 28

5.3.4 Menu functions: setting parameters of refrigeration, time and preset counter .................... 29

5.3.5 Status display .................................................................................................................................... 32

5.3.6 Indication of refrigeration state, section thickness setting and counters .............................. 33

5.4

Control panel 2 .................................................................................................................................. 34

5.4.1 Selecting a sectioning mode / ........................................................................................................ 35

5.4.2 Setting the sectioning window....................................................................................................... 36

5.4.3 Selecting sectioning speed ............................................................................................................ 37

5.4.4 Emergency stop ................................................................................................................................ 37

5.4.5 Coarse feed keys .............................................................................................................................. 38

5.4.6 Trimming and sectioning functions ............................................................................................... 39

5.4.7 Setting the section thickness ......................................................................................................... 39

5.5

Daily operation of the instrument .................................................................................................. 40

5.5.1 Freezing specimens onto specimen .............................................................................................. 40

5.5.2 Freezing specimens onto specimen .............................................................................................. 41

5.5.3 Optional accessories for freezing .................................................................................................. 41

5.5.4 Inserting specimen discs into the .................................................................................................. 42

5.5.5 Specimen orientation....................................................................................................................... 42

5.5.6 Trimming / sectioning ...................................................................................................................... 43

5.6

Finishing work ................................................................................................................................... 44

5.6.1 Daily routine steps............................................................................................................................ 44

5.6.2 Switching off the instrument for a ................................................................................................. 44

5.7

Defrosting .......................................................................................................................................... 45

5.7.1 Automatic chamber defrost cycle ................................................................................................. 45

5.7.2 Manual chamber defrost cycle ...................................................................................................... 46

5.7.3 Defrosting the specimen head ................................................................................................ 46

Leica CM3050S – Cryostat

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Table of contents

Troubleshooting, applications tips ....................................................................................... 47

6.1

6.1.1 Other error indications .................................................................................................................... 47

6.2 Potential problems - causes and remedies ................................................................................. 48

Displayed error messages .............................................................................................................. 47

6.3 Reference chart for temperature settings (in minus °C) .................................................... 52

Disinfection, cleaning and maintenance ............................................................................ 53

7.1

7.2

7.3

7.4

7.5

Safety instructions ........................................................................................................................... 53

Spray disinfection with Leica Cryofect ......................................................................................... 53

Disinfection with conventional ....................................................................................................... 54

Cleaning ............................................................................................................................................. 54

Removing / reinstalling the microtome ......................................................................................... 55

7.5.1 Removing the microtome ................................................................................................................ 55

7.5.2 Disassembling the specimen head................................................................................................ 55

7.5.3 Reinstalling the microtome ............................................................................................................. 56

7.6 Exchanging the fluorescent light lamp................................................................................... 57

Warranty and service .............................................................................................................. 58

Appendix .................................................................................................................................... 59

Electrohydraulic height adjustment .............................................................................................. 59

Ordering information - accessories ....................................................................................... 62

10. EC Declaration of Conformity ..................................................................................... 63

11. Decontamination Certificate (master) .................................................................................... 64

Instruction Manual V1.2 - 11/2006

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1. Important information

The chapters of the Leica CM3050 S cryostat 1.1 Symbols used in this manual and

their meaning

instruction manual:

Warnings

appear in a grey box and are marked

by a warning triangle

Chapter 1 Structure of the manual:

•

Table of contents

Important information on this

manual

Chapter 2 Safety

Read this chapter before

operating the instrument!

Chapter 3 Installation

Notes

i.e. important user information

appears in a grey box and is marked

•

by an information

symbol.

•

Unpacking and installation

Standard delivery, information

on accessories

Figures in brackets refer to item

numbers in drawings or to the

drawings themselves.

(5)

(Fig.5)

Chapter 4 Instrument properties

•

Overview

Technical data

Instrument type:

All information given in this instruction manual

applies only to the instrument type indicated on

the title page.

Chapter 5 Operation

•

Controls

Setup, daily use and shut

down

A name plate, indicating the instrument serial

number, is attached to the back of the instru-

ment.

Chapter 6 Trouble shooting

and applications tips

•

Operating errors

Required in-

formation for

all inquiries:

Display error messages

Temperature reference chart

For any inquir-

please

Chapter 7 Disinfection, cleaning

and maintenance

ies

specify:

•

Instrument

type

Chapter 8 Warranty and service

Chapter 9 Electrohydraulic height adjustment

Ordering information accessories

EC Declaration of Conformity

•

Serial-

number

Leica CM 3050 S – Cryostat

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1. Important information

General

1.2 Designated use

This instruction manual includes important

instructions and information related to the

operating safety and maintenance of the

instrument.

The Leica CM3050S is a powerful cryostat for

routine as well as research applications in

biology, medicine and industry.

The instrument has been designed for rapid

freezing and sectioning of tissue samples.

The instruction manual is an important part of the

product. It must be read carefully before using

the instrument for the first time and must always

be kept with the instrument.

The instrument has not been designed for un-

attended storage of tissue material.

The instrument may only be operated within the

scope of its designated use as described above

and as per the instructions given in this manual.

If additional requirements, which exceed the

scope of this manual, are imposed by regula-tions

and/or laws on accident prevention and

environmental protection in the country of

operation, appropriate instructions for

compliance with such requirements must be

added to this manual.

Any other use of the instrument is considered

improper.

Read this instruction manual carefully before

attempting to use or operate the instrument.

1.3 Qualification of personnel

The Leica CM3050S may only be operated by

trained laboratory personnel.

Please pay particular attention to

(safety features, safety

Please read this

information, even if you are already

familiar with the operation and use of

other Leica products.

All laboratory personnel designated to operate the

instrument must carefully read the present

instruction manual prior to starting work with the

instrument.

chapter

instructions).

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2. Sicherheit

2.1 General information on instrument

design and safe handling

2.2 Integrated safety devices

The instrument is equipped with the following

safety devices:

This instrument has been built and tested in ac-

cordance with the following safety regulations

on electrical measuring, control, regulating and

laboratory devices:

•

Handwheel lock

Handwheel grip centering

Emergency stop function

•

DIN EN 292

DIN EN 61010-1

EN 50082-1

EN 55011

(instruments with sectioning motor only)

Knife holder equipped with knife guard

•

The safety devices installed by the manufactu-

rer of the instrument only constitute the basis of

accident prevention.

IEC 1000-4

Mainly responsible for accident-free operation

is above all the institution which owns the in-

strument and, in addition, the designated per-

sonnel who operate, service or repair the

instrument.

as well as according to the international quality

standard

•

DIN ISO 9001

In order to maintain this condition and to ensure

safe operation, the operator must observe the

instructions and warnings contained in this in-

struction manual.

Leica CM3050 S – Cryostat

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2. Safety

Instruments with sectioning motor:

2.2.1 Locking the handwheel

The sectioning motor is now blocked.

Always cover the cutting edge with

the knife guard and lock the hand-

wheel:

All instruments:

•

Prior to doing any work on

knife and/or specimen.

Prior to exchanging specimens.

During work breaks.

The message ‘LOCKED’ in the display of control

panel 1 indicates that the handwheel has been

locked:

•

C T - 3 0 ° C

O T - 3 5 ° C

L O C K E D

The handwheel can be locked in 2 positions:

•

with the grip in the uppermost position (left),

with the grip in the lowest position (right).

Unlocking:

•

To unlock, push locking pin (2) to the left into

position (2a).

•

Display indication ‘LOCKED’ disappears.

Instruments with sectioning motor:

The sectioning motor can now be activated

again.

2.2.2 Centering the handwheel grip

During motorized sectioning, for

safety reasons always center the

handwheel grip!

Locking:

•

Rotate handwheel, until grip (1) is in upper or

lower position.

To lock, press pin (2) to the right into position

(2b).

•

To center grip (1), pull outwards and pivot

into center of handwheel.

When released, the grip locks into position.

--> The upper locking position for pin (2) is

marked by a black dot (4).

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Safety

2.2.3 Emergency stop function

(instruments with sectioning

motor only)

2.2.4 Knife guard

All knife holders are equipped with a knife

guard (--> see separate instruction manuals on

knife holders).

The emergency stop is activated via the red

emergency stop button in control panel 2 or via

the footswitch

Always cover the cutting edge with

the knife guard:

• Prior to doing any work on knife

and/ or specimen.

• Prior to exchanging specimens.

• During work breaks.

Control panel 2

Footswitch

Activating the emergency stop function

•

Press emergency stop button or step on

footswitch forcefully.

-->

STOP (red) lights up.

As soon as the emergency stop function is acti-

vated, the sectioning motor stops.

Deactivating the emergency stop

•

To deactivate, rotate emergency stop button

in direction of arrow.

If the emergency stop function has

been activated by the footswitch,

unlocking is not necessary (func-tion

is unlocked as soon as the footswitch

is released).

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2. Safety

2.3 Safety instructions: handling and operation of the instrument

2.3.3 Electrical connections

2.3.1 Transport

•

Do not use extension cords for connecting

the instrument to mains.

-->Fire hazard!

•

To avoid severe damage to the instrument by

running it while the compressor oil is dis-

placed from its regular position:

-->Instrument malfunctions caused by

voltage drop.

•

Do not tilt the instrument,

only transport in an upright position.

After transport, wait at least 4 hours be-

fore turning the instrument on.

•

During the start-up phase of the compressor,

the nominal voltage must not drop below the

values specified in chapter 4.2 ‘Technical

data’!

-->The compressor needs a start-up current

at between 25 and 35 A (see chapter 4.2

‘Technical data’)

-->The compressor oil may have been

displaced during transport and must

settle to its original position

be-

fore switchingthe instrument on.

Otherwise, the instrument may be

severely damaged.

•

Ensure uniform current supply according to

specifications.

2.3.2 Site requirements

-->Electrical power supply deviating from

specifications damages the instrument.

•

Do not operate the instrument in rooms with

explosion hazard.

•

Therefore, arrange for the electrical in-

stallations on site to be checked by a

trained professional and make sure any

necessary upgrades are installed!

Have the circuit protected by a fuse of its

own!

•

To ensure proper instrument function main-

tain a minimum distance of 10 cm between

walls and/or furniture and all sides of the in-

strument!

•

Do not connect any other consumers to

the same circuit.

Prior to connecting the instrument to

mains, make sure the electrical power

supply in your laboratory corresponds to

the values indicated on the instrument

nameplate (located at the rear of the in-

strument)!

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2. Safety

2.3.4 Handling microtome knives/blades

2.3.7 Defrosting/Handling frozen tissue

•

Danger!

-->Microtome knives and disposable blades

have extremely sharp cutting edges and

can cause serious injuries. Therefore:

•

Never leave specimens unattended in the

cryochamber over an extended period of

time!

•

Handle knives / blades with utmost care.

Never leave any knives / blades in unpro-

tected places!

Never place a knife, no matter where,

with the cutting edge facing upwards.

Never try to catch a falling knife.

Always insert the specimen before

inserting the knife.

-->In case of power failure or instrument

failure, or during the automatic defrost cy-

cle, tissue material can be destroyed.

During the defrost cycle the cryochamber is

partially warmed. - Therefore:

•

Remove sensitive specimens from the

chamber prior to defrosting.

•

If automatic defrosting is programmed to

take place during the night, remember to

remove all specimens from the cryo-

chamber prior to leaving work.

2.3.5 Knife guard/handwheel lock

•

Always cover the cutting edge with the knife

guard and lock the handwheel:

2.3.8 Frozen parts of the instrument and

frozen accessories

•

Prior to doing any work on knife and / or

specimen.

•

Prior to exchanging specimens.

During work breaks.

•

Prolonged contact of bare skin to frozen

surfaces of the instrument or to frozen

accessories (specimen discs, knife holder,

shelves etc.) can cause frostbite.

2.3.6 Motorized sectioning

•

Wear protective gloves.

•

Do not interrupt sectioning / trimming by set-

ting the sliding potentiometer to zero speed!

-->This does not really switch the sectioning

function off - it only operates at ‘0’ speed.

If the sliding potentiometer is accidental-

ly moved, the instrument will resume sec-

tioning immediately (risk of injury)!

•

During motorized sectioning, always center

the handwheel grip!

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2. Safety

2.3.9 Infectious/radioactive material

2.3.10 Disinfection and cleaning

•

Prior to disinfection, switch the instrument

off and unplug it from mains.

•

Danger!

Use caution when working with potentially

infectious specimens:

-->Risk of infection!

•

For removal of the microtome from the cryo-

chamber see chapter 2.3.11 ‘ Removing the

microtome’.

•

When working with potentially infectious /

radioactive specimens:

•

For disinfection, wear protective gear:

(gloves, mask, lab coat etc.)!

•

Wear protective clothes (gloves, protec-

tive boots, mask, lab coat), in compliance

with radiation safety regulations and/or

in-house regulations on handling infec-

tious / radioactive material.

For disinfection, only use alcohol-based dis-

infectants!

Do not use solvents (xylene, acetone etc.)

for cleaning or disinfection!

•

When working with radioactive specimens:

Do not spray disinfectants into the evapora-

tor!

-->Risk of icing!

•

Comply with applicable radiation safety

regulations!

•

Dispose of radioactive specimen waste ac-

cording to applicable regulations.

•

Explosion hazard when working with alcohol:

Make sure the premises are appropriately

ventilated!

When using disinfectants and detergents,

comply with all safety instructions supplied

by the manufacturer of the product!

•

Dispose of waste liquids from disinfection/

cleaning as well as of sectioning waste ac-

cording to applicable regulations on disposal

of special category waste!

•

Disinfected accessories must be thoroughly

dry when reinserting them into the chamber!

-->Risk of icing!

Make sure the chamber is completely dry

before switching the instrument back on:

-->Explosion hazard through alcohol vapors!

-->Risk of icing!

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2. Safety

2.3.11 Removing/reinstalling the

microtome

2.3.12 Display message ‘Dry microtome’

•

If the error message ‘Dry Microtome’ is dis-

played in control panel 1, the following has

happened:

•

Before removing the microtome:

Switch instrument off.

•

Cryochamber refrigeration has been in-

terrupted for an extended period of time

(e.g. power failure), causing the chamber

temperature to rise into the positive

digits.

Unplug from mains.

Place handwheel grip in lowest position and

lock.

-->When removing the microtome, the

specimen head must always be lok-

ked in the lowest position.

Otherwise the upper part of the slot

cover might be bent and consequently

damaged!

• If this message appears, do not switch on

the instrument but remove the microtome

from the chamber, disinfect, if necessary,

and dry thoroughly before reinstalling it

into the chamber (see chapter 7.1 to 7.5).

•

When removing the microtome:

2.3.13 Maintenance

•

Wear gloves when removing the micro-

tome while it is still frozen.

-->Risk of frost bite!

On instruments with specimen cooling:

do not distort the refrigerating tube!

If distorted it might break, causing ex-

•

Only technical service engineers authorized

by Leica may access the internal compo-

nents of the instrument for service and re-

pair.

•

The fluorescent light lamp (chamber illumi-

nation), unless broken or splintered, can be

replaced by the user:

tremely cold refrigerant to escape.

> Risk of frost bite!

- -

•

Before reinstalling the microtome:

•

Switch off mains switch!

•

Microtome must be completely dry.

>Humidity in the interior of the micro-

tome freezes and causes

Unplug the instrument from mains!

If the lamp is broken or splintered:

Have lamp replaced by Leica Technical

Service!

microtome malfunctions and/or

damage to the microtome.

-->Risk of injury!

•

Use only those replacement lamps that cor-

respond to technical specification (see

chapter 4.2 ‘Technical Data’).

•

All accessories/tools removed from the

cryochamber must be thoroughly dry be-

fore putting them back into the chamber!

-->Risk of icing!

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3. Installation

3.1 Site requirements

3.2 Unpacking and installation

Make sure to read and follow all

safety instructions in chapter 2.3.2

‘Site requirements’!

Unpacking instructions are always located in a

transparent protective envelope on the outside

of the instrument shipping crate.

3.1.1 General site requirements

Make sure to read and follow all

safety instructions provided in

chapter 2.3.1 ‘Transport’ and on the

unpacking instructions!

•

No direct sunlight.

Electrical power supply within distance

(length of power cord = approx. 4 meters -

do not use extension cords!).

3.2.1 Repacking

•

No draft (caused by air conditioning etc.).

Even floor surface.

We recommend to keep the original shipping

crate and the unpacking instructions for the

Leica CM3050 S.

Practically vibration-free floor.

Handwheel easily accessible.

For repacking, proceed as per unpacking in-

structions, in reverse order.

Room temperature constantly below +22 °C.

Relative humidity of air maximum 60 %.

Available models

High ambient temperature and/or high

air humidity negatively affect

instrument cooling performance!

•

Basic instrument

- with sectioning motor

- without specimen cooling

3.1.2 Electrical connections

Basic instrument

- without sectioning motor

- with specimen cooling

Make sure to read and follow all

safety instructions in chapter 2.3.3

‘Electrical connections’!

Basic instrument

- with sectioning motor

- with specimen cooling

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3. Installation

3.3 Standard delivery

Cryostat with microtome ............................................................................. 0460 32037

Handwheel with marking, antibacterial ...................................................0471 42558

Heat extractor, stationary...........................................................................0369 11197

Low-temperature stabilizer for heatextractor ........................................0452 27919

90° prism for direct specimen freezing on specimen head

with clamping screw to facilitate specimen trimming .......................... 0443 25949

Set of specimen discs .................................................................................0470 43550

- 4 Specimen discs, 25 mm .........................................................................0416 19275

- 4 Specimen discs, 30 mm .........................................................................0370 08587

Storage shelf, right ...................................................................................... 0443 25723

Storage shelf, left ......................................................................................... 0443 33549

Section waste tray .......................................................................................0400 26817

Foot switch with protective guard ............................................................0502 29977

Rubber mat .................................................................................................... 0443 25732

Freezing shelf cover ....................................................................................0443 30783

Brush shelf .................................................................................................... 0398 13088

Tool set........................................................................................................... 0436 43463

-1 Brush, fine ................................................................................................. 0183 28642

-1 Leica-brush ............................................................................................... 0183 30751

-1 Allen key, size 1.5 .....................................................................................0222 10050

-1 Allen key, size 2.5 .....................................................................................0222 04137

-1 Allen key, size 3.0 .....................................................................................0222 04138

-1 Allen key, size 4.0 .....................................................................................0222 04139

-1 Allen key with spherical head, size 4.0 ................................................0222 32131

-1 Allen key, size 5.0 .....................................................................................0222 04140

-1 Allen key with handle, size 5.0 ...............................................................0194 04760

-1 Allen key, size 6.0 .....................................................................................0222 04141

-1 Single-head wrench, size 13..................................................................0330 33149

-1 Single-head wrench, size 16..................................................................0330 18595

Bottle of OCT-Compound, mounting medium

for cryosectioning, 125 ml...........................................................................0201 08926

Bottle of cryostat oil, type 407, 50 ml ........................................................0336 06098

Pair of cut resistant gloves, size S ............................................................0340 40859

Instruction manual Leica CM3050 S - G/E/F/S ........................................0708 37109

In addition to the above:

Instruments with specimen cooling:

Configured instruments:

Instruments with sectioning motor:

90° Prism

for low temperature quick freezing

Knife holder base

Knife holder with accessories

Footswitch with

protective guard

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Installation

In addition to the above:

•

Instruments with specimen cooling:

90° Prism for low temperature quick freezing

Configured instruments:

•

Knife holder base

Knife holder with accessories

•

Instruments with sectioning motor:

Footswitch with protective guard

Further accessories:

Further accessories which you ordered will be included in the box containing

the standard delivery items.

Knife holders are delivered with anti-roll guide, knife guard, and a

separate instruction manual.

In case of non-configured instruments, the knife holder is not a

part of standard delivery but must be ordered separately.

•

Check all delivered parts against the packing list and against your order

to verify whether the delivery is complete!

If there is any difference, contact your local Leica office immediately.

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3. Installation

3.4 Installing/inserting accessories

3.4.1 Installing the handwheel

3.4.2 Inserting the accessories

•

Place the rubber mat on top of the housing.

Insert the storage shelves into the cryo-

chamber.

•

Insert pin (1) of the handwheel shaft into

hole (2).

•

Install the stationary heat extractor into the

quick-freeze shelf (see also drawing in

chapter 5.5.1 ‘Freezing specimens onto

specimen discs’).

•

Place spring washer (3) onto screw (4) as

shown.

•

Tighten screw (4) with an Allen key.

Cover with selfadhesive disc (5).

•

Insert the low temperature stabilizer into the

quick freeze shelf (it must be located in the

pivoting range of the heat extractor - see

also drawing in chapter 5.5.1 ‘Freezing spec-

imens onto specimen discs’).

•

Insert section waste tray and brush shelf.

Install knife holder base onto microtome

base plate and clamp.

•

Install knife holder and clamp (see knife

holder instruction manual for details).

Place knife case with knife into chamber to

precool.

Place all tools needed for section prepara-

tion into the chamber.

For purposes of transport (e.g. narrow

doors), the handwheel can be

removed.

Close the sliding window.

For

individual parts:

--> see Chapter 4.1 ‘Overview’.

complete overview of all

•

To remove the handwheel, proceed as de-

scribed above but in reverse order.

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3. Installation

3.4.3 The footswitch

Connecting the footswitch

•

Insert footswitch into port (1) and secure.

Function

The footswitch performs the same task as the

RUN/STOP and RUN/ENABLE keys (activating/

deactivating motorized sectioning / trimming).

In addition, the footswitch can be used to acti-

vate the emergency stop function.

3.5 Prior to switching on the instrument

After transport, observe a waiting

period of at least 4 hours before

turning the instrument on! - See also

safety instructions in chapter 2.3.1

‘Transport’.

Models with footswitch:

Have you observed all safety

instructions in chapters 2.3.2 ‘Site

requirements’ and 2.3.3 ‘Electrical

connections’?

All instruments with sectioning motor.

If not:

--> Please read chapters 2.3.2 and

2.3.3!

Important note:

In all instrument models that are

delivered with footswitch, the

footswitch must be installed!

Otherwise the instruments are not

functional.

•

Insert mains plug into wall outlet.

Continue with chapter 5.1 ‘Setting up the in-

strument’.

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4. Instrument Properties

4.1 Overview

Cryostat CM3050S

Control panel 1

Control panel 2

Storage shelf, left

Storage shelf, right

Rubber mat

Mains switch /

Automatic cutout

for instrument,

Automatic cutout

for sectioning motor,

Footswitch port

Quick-freeze shelf

Stationary

heat extractor

10 Mobile heat extractor

11 Specimen disc

12 Thermoblock (optional)

13 Section waste tray

14 Brush shelf

15 Specimen head w/o

specimen cooling

16 Specimen head with

specimen cooling

(Option)

17 90° Prism

(instruments with

specimen cooling

only)

18 Knife holder base

19 Knife holder CE

20 Knife holder CN

21 Knife holder CS

22 Footswitch with

protective guard

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4. Instrument Properties

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4. Instrument properties

4.2 Technical Data

Operating temperature range (ambient temperature): + 18°C to + 40°C. All specifications related to tempe-

rature are valid only up to an ambient temperature of + 22°C and relative air humidity of less than 60%!

Type

CM3050S-10

CM3050S-1

CM3050S-8

CM3050S-9

Marks of conformity

Nominal voltage

Nominal frequency

Power draw

100 V AC 10% 230 V AC 10% 240 V AC 10% 100 V AC 10%

50 Hz

1800 VA

50 Hz

1800 VA

25 A eff.

50 Hz

1800 VA

25 A eff.

60 Hz

1800 VA

30 A eff.

Max. start-up current for 5 sec 35 A eff.

Protective fuse

Automatic cutout

T15A M3

T10A T1

T15A M3

Pollution degreeꢀ

Overvoltage installation category II

1800 J/s

Heat emission (max.)

1800 J/s

Refrigeration system

50 Hz

60 Hz

Cryochamber

Temperature selection range

Defrosting

0° C to -40°C (+ 2 K / - 0 K)

Automatic hot-gas defrost cycle,

0° C to -40°C (+2 K / - 0 K)

Automatic hot-gas defrost cycle,

programmable in 15-min. increments;

1 automatic defrost cycle per 24 hours.

Duration: 6 - 12 minutes

Manual defrost cycle

programmable in 15-min increments;

1 automatic defrost cycle per 24 hours.

Duration: 6 - 12 minutes

Manual defrost cycle

690 W

Refrigeration capacityꢀ

Safety factor

Refrigerant

690 W

280 g 5g refrigerant R 404A *

280 g 5g refrigerant R 404A*

Compressor oil

0.6 l EMKARATE RL-22S, ICI *

Quick-freeze shelf

Maximum temperature

Number of quick-freeze stations 10

- 43°C (+ 0 K / - 2 K)

Specimen cooling (Option)

Temperature range

Defrosting

Refrigeration capacityꢀ

Safety factor

-10° C to -50°C 3K

Manual defrost cycle (electric)

320 W

-10° C to -50°C 3K

Manual defrost cycle (electric)

320 W

Refrigerant

Compressor oil

185 g 5g refrigerant R 404A *

0.4 l alpha 22, Kyodo *

or RENISO E22, Fuchs *

185 g 5g refrigerant R 404A *

0.4 l alpha 22, Kyodo *

or RENISO E22, Fuchs *

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4. Instrument properties

*) Refrigerant and compressor oil to be replaced only by trained and authorized service

personnel!

CM3050S-3

c-UL

120 V AC 10% 208 V AC 10% 230 V AC 10%

CM3050S-6

CM3050S-7

Microtome

Rotary microtome

Section thickness setting

Overall specimen feed

Vertical stroke

0.5 - 300 μm

25 mm

59 mm

60 Hz

1800 VA

35 A eff.

60 Hz

1800 VA

30 A eff.

60 Hz

1800 VA

25 A eff.

Specimen retraction

Maximum specimen size

Specimen orientation

50 μm

40 x 55 mm

8° (x-, y-, z-axis)

T15A T1

T12A T1

T10A T1

Trimming (6 thickness settings)

Motorized coarse feed

5 - 150 μm

1800 J/s

- slow

- fast

500 μm/s

1,000 μm/s

Sectioning motor (Option):

Sectioning speed

- slow

Fluorescent light lamp (cryochamber illumination):

min. 0.1 mm/s

max. 100 mm/s

50 Hz version:

60 Hz version:

Osram Dulux S 11 W/21

Light color ‘LUMILUX hellweiß’

min.

210 mm/s

0.1 mm/s

(brilliant white)

max

- fast

Osram Dulux S 13 W/21

Light color ‘LUMILUX hellweiß’

max. 170 mm/s

210 mm/s

(brilliant white)

max

Cryocabinet

Dimensions

according to IEC-1010, UL 3101

Width (w/o handwheel)

Width (including handwheel)

Depth

800 mm

882 mm

766 mm

840 mm

1040 mm

according to CECOMAF:

Liquid temperature 45°C

Evaporation temperature -25°C

Height (arm rest level)

Overall height

Weight

(incl. microtome, w/o specimen cooling)

ca. 180 kg

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5. Operation

5.1 Operating the instrument for the first time

Prior to setting up the instrument, make sure to read the instruction manual

carefully and in full.

When setting up the instrument, please follow the individual chapters in the

following order:

•

First, study chapters 1, 2, and 3 - install the instrument as described in

chapter 3.

The overview in chapter 4.1 is helpful during the unpacking and instal-

lation procedure in order to identify individual parts and place them in

the correct location.

•

Go on to chapter 5.

Read chapters 5.1, 5.2, 5.3 and 5.4 to learn all about the controls.

Next, start to work with the instrument (chapter 5.5). Follow the instruc-

tions in chapter 5.5.

Chapter 5.5.2 applies to instruments with specimen cooling only.

The temperature chart in chapter 6.3 is useful when it comes to select-

ing appropriate sectioning temperatures for different types of speci-

mens.

•

When disinfecting / cleaning the instrument and/or removing / rein-

stalling the microtome, please follow the instructions in chapter 7.

In case of problems and malfunctions, please refer to chapters 6.1 and

6.2. These chapters list the most common types of problems which

many times have simple causes that can be eliminated by the actual

user.

If you find ‘Call Technical Service’ in the ‘Remedy’ column, please do

so. Never try to solve these problems yourself!

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5. Operation

Automatic cutout for sectioning motor:

5.2 Switching on/fuses

The instrument is equipped with an automatic

cutout (2) for the sectioning motor.

If the sectioning motor is overloaded (e.g. when

sectioning extremely hard specimens etc.) the

automatic cutout (2) will be triggered.

To make the instrument functional again after

the automatic cutout (2) has been triggered:

(Automatic cutout

for sectioning

motor)

•

Push automatic cutout (2) inwards until it

locks into position.

(Combination Mains switch /

automatic cutout for the

instrument)

Switching on

•

To switch on the instrument, place switch (1)

in upper lock-in position.

Mains switch / automatic cutout for the

instrument:

In addition to its function as ON/OFF switch,

switch (1) is also the automatic cutout for the in-

strument.

If the automatic cutout has been triggered (e.g.

caused by overcurrent):

•

Put switch (1) back in upper lock-in position.

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5. Operation

5.3 Control panel 1

5.3.2 Display functions in control panel 1

5.3.1 Key functions in control panel 1

The display in control panel 1 combines several

different functions:

ON/OFF key for cryochamber illumination.

1. When switching the instrument on:

To select the menu items for setting /

changing instrument parameters.

A series of standard indications is displayed,

during which two basic parameters can be se-

lected (with the arrow keys):

Language (of display indications) and speed

level of sectioning motor.

See chapter 5.3.3 ‘Display indications when

switching on’.

In the status indication, pressing the ar-

row keys displays the individual current

parameters.

In the menu, pressing the arrow keys

changes set values.

2. Menu function (activated via menu key):

Via the menu key a number of parameters can

be selected, then be activated /deactivated via

the lock/unlock key and/or changed via the ar-

row keys.

As soon as a value is displayed, it is au-

tomatically stored.

Activates the manual defrost cycle (cry-

ochamber). This key is functional only

when one of the menu items ‘SET TEMP

CT ... °C’ or ‘SET TEMP OT ... °C’ (last

one only applies to instruments with

specimen cooling) has been selected

first.

See chapter 5.3.4 ’Menu functions: Setting param-

eters of refrigeration, time and preset counter’.

3. Status indication function:

The actual status of selected parameters is in-

dicated in the display screen.

Some of those parameters are displayed auto-

matically (e.g. sectioning stroke phase, coarse

feed position, handwheel locked).

Others can be displayed by pressing the arrow

keys; e.g. selected section thickness, preset

counter status etc.

See chapter 5.3.5 ‘Status indication’ and chap-

ter 5.3.6 ‘Displaying state of refrigeration, sec-

tion thickness and counters’.

Lock / unlock key:

- Locks the display to protect against

accidental changes of set parameters.

- Activates / deactivates a number of

functions.

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5. Operation

5.3.3 Display indications when switching on

Meaning of indication:

Operator activity:

---

Initialization phase 1

8 8 8 8 8 8 8 8 8 8 8 8 8 8 8 8

ꢀ

Initialization phase 2

---

C M 3 0 5 0 S

V s . X X . X X

Software version

---

E N G L I S H

Selected display language

Select language via

keys

C U T T I N G M O T O R

S L O W

Sectioning motor speed

level ‘slow’ / (fast)

Select speed level via

keys

Temperature prior to

switching off < 0 °C

yes

Temperature upon

switching on ≥ 0 °C

C T . . . . ° C

P O W E R

O T .

. . ° C * )

F A I L U R E

yes

Acknowledge indication by

pressing once briefly.

C T . . . . ° C

O T .

. . ° C * )

C T . . . . ° C

O T .

. . ° C * )

D R Y M I C R O T O M E

1 2 : 0 0

Standard indication ‘locked’ is displayed:

(Display background dark)

If this indication is displayed: Do not switch on

the instrument!

To unlock: press

ground lights up:

until the display back-

Prior to switching on, both microtome and

accessories must be completely dry!

Remove microtome/accessories, disinfect if

applicable, then dry thoroughly and reinstall.

(--> See chapters 7.1 - 7.5).

C T . . . . ° C

O T .

. . ° C

ꢀ

H O M E

❄ ❄

*) Indication in instruments with specimen cooling. Instruments without specimen cooling: only CT (centered) is displayed.

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5. Operation

5.3.4 Menu functions: setting parameters of refrigeration, time and preset counter

Once the instrument has gone through the initial

sequence of indications, you can go on to the

menu functions to select and/or change instru-

ment parameters.

1. Specimen temperature:

(instruments with specimen cooling only)

Press key until menu item ‘Set specimen

temperature’ is displayed:

If display is locked:

Press key until display background

lights up.

S E T

T E M P O T - - - ° C

- -

Press key briefly.

The line in the specimen temperature

field is replaced by an asterisk.

Next, press the menu key. With each key stroke

the following parameters will be displayed (dis-

play sequence as listed):

S E T

T E M P O T - - - ° C

- ❄

1. Specimen temperature

(in instruments with specimen cooling only)

(-10 - -50 °C)

From this point on the refrigeration sys-

tem is activated and the desired set

temperature can be selected with one

of the arrow keys.

2. Cryochamber temperature

(0 - -40 °C)

Press arrow key and release upon

reaching the desired value.

3. Selected number of sections - preset

counter

(ON/OFF and setting selection from 1 to 999)

C T . . . . ° C

H O M E

O T - 3 5 ° C

ꢀ

- ❄

4. Maximum specimen temperature

(ON/OFF)

Note on the arrow key function:

Each time one of the arrow keys is pressed

briefly, the value displayed in the menu will be

changed one step up or down.

If an arrow key is pressed and held, the value

will change continually (auto-repeat function).

When holding the key, scrolling speed will in-

crease after a while.

5. Real time

(in 1-minute steps)

6. Defrost time (starting time of automatic

cryochamber defrosting)

(in 15-minute steps)

7. Duration of defrost cycle

(cryochamber defrosting)

(6 to 12 minutes duration)

As soon as a value is displayed, it is at the same

time automatically stored.

Menu items 5 and 6 displayed on 24 hour clock.

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5. Operation

Now there are two options:

1. Increase or decrease the actual reference

value by pressing or

2. Press and simultaneously in order to

2. Cryochamber temperature:

Press key until menu item ‘Set chamber

temperature’ is displayed:

--> Continue as described for

specimen temperature.

reload the former reference value (‘0’ when

setting up the instrument) and then press ei-

3. Preset counter

(instruments with sectioning motor only):

ther

individually in order to select

the desired reference value. Depending on

where you start from and where you want to

go, method 1 or method 2 is quicker (see ex-

amples).

Press key until menu item ‘Preset

counter’ is displayed:

P R E S E T

C O U N T E R

d n - - -

Example 1:

= Preset counter activated

P R E S E T

C O U N T E R

d n 0 5 6

Actual preset counter reading = 50

Former reference value = 300

New reference value of 65 to be selected.

= Preset counter deactivated

Method 1 is most convenient:

Preset counter function:

•

Increase from 50 to 65 by pressing

The preset counter allows the user to carry out

a predefined number of sections in the ‘Continu-

ous stroke’ sectioning mode.

Example 2:

Once the preselected number of sections has

been carried out (= section counter reading ‘0’),

the specimen head will stop automatically in the

lower limit position.

Actual preset counter reading = 50

Former reference value = 300

New reference value of 320 to be selected.

Method 2 is most convenient:

The reference value (i.e. the value from which

the preset counter counts downward) can be

selected in the corresponding menu item.

•

Press

reload old reference value (300):

• Increase from 300 to 320 by pressing

and

simultaneously to

In order to be able to set a reference value, the

preset counter function must be active.

For further information on the preset counter,

please refer to chapter 5.3.6, Item 4 ‘Preset

counter’.

If the preset counter is deactivated (see diagrams

above), press

to activate.

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5. Operation

The automatic defrost cycle takes place once

every 24 hours at the selected defrost time.

4. Quick-freezing at maximum specimen

temperature* (instruments with specimen

cooling only)

7. Duration of defrost cycle:

Press key until menu item ‘Fast object

freezing’ is displayed:

Press key until menu item ‘Defrost period’

is displayed:

F A S T

O B J

O F F

F R E E Z I N

D E F R O S T

P E R I O D

C H A M B E R :

0 8

M I N

F A S T

O B J

O N

F R E E Z I N

•

Use defrost keys (

) to adjust de-

frost time. (Only if necessary - see below).

Press lock/unlock key to activate or de-

activate (ON/OFF).

Important information for setting the

defrost parameters

Starting time of defrost cycle:

5. Real time

We recommend to program the

automatic defrost cycle for a time out-

side regular working hours as a

period of 4 hours should pass after the

defrost cycle before work with the

instrument is resumed (see also

chapter 5.7.1 ‘Automatic chamber

defrost cycle’).

Press key until menu item ‘Time’ is dis-

played:

T I M E

1 0 : 2 3

•

Use arrow keys (

) to set real time.

Setting minutes as well as hours in

1-minute steps. Holding arrow button in-

creases scrolling speed!

Defrost cycle duration:

The duration of the chamber defrost

cycle is set to 8 minutes in factory.

This setting should only be

changed, if the evaporator is not

completely de-iced during the

8-minute cycle.

In such cases, increase the defrost

cycle duration gradually until the the

evaporator is ice-free after defrosting.

The selected defrost cycle duration is

valid for both automatic and

manual chamber defrost cycle.

6. Starting time of automatic defrost cycle:

Press key until menu item ‘Defrost time’ is

displayed:

D E F R

T I M E

4 : 3 0

(Time on display = start time of defrost

cycle)

•

Use arrow keys (

sired defrost start time.

) to select the de-

*) with 90° prism - see also chapter 5.5.2.

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5. Operation

5.3.5 Status display

Each individual parameter is displayed in a certain area of the display screen.

Upper line:

In the menu (see chapter 5.3.4): indication of selected menu item.

Standard indication: Chamber temperature (instruments with specimen cooling: chamber and specimen temperature)

Standard indication upper line:

Instruments with chamber and specimen cooling

Instruments with chamber cooling only

C T - 3 0 ° C

H O M E

O T - 3 5 ° C

ꢀꢀ

ꢀ

H O M E

ꢀ -

ꢀ

Upper line

Lower line

1 0 1 1 1 2 1 3 1 4 1 5 1 6

Digits 1 and 2

indicate sectioning stroke position:

Digits 3 - 6

Digits 7 - 16

indicate coarse feed position:

indicate the following parameters:

•

Sectioning stroke

(outside sectioning window)

• Rear limit position:

• Front limit position:

HOME

STOP

• Refrigeration status:

Digit 15 = cryochamber

Digit 16 = specimen

Meaning of

•

ꢀ

Retraction stroke

Indication of ‘malfunction’ or

‘handwheel locked’:

Refrigeration OFF

• [ ]:

Sectioning stroke

refrigeration

symbols:

(inside sectioning window)

• Microtome plug loose,

implausible values etc.:

ꢀ

Refrigeration ON

Defrosting active

????

Sectioning

stroke

ꢀ

• Handwheel is locked:

LOCKED

• Section thickness

(set value)

Indication ‘LOCKED’ completely

overlays digits 3 through 16,

Sectioning window [

]

i.e. while ‘LOCKED’ is displayed, no

other parameters will be displayed

in digits 3 - 16 of the lower line.

• Section counter

(actual value)

• Indication of total section thickness

(actual value)

• Further indications: see chapter 6.1

‘Error messages indicated in the

display screen’

ꢀꢀ

Retraction stroke

• Preset counter

Digits 7 - 16:

Scroll via arrow buttons to display

individual parameters

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5. Operation

5.3.6 Indication of refrigeration state, section thickness setting and counters

These parameters can be displayed via the ar-

row keys.

4. Total section thickness counter

C T - 3 0 ° C

O T - 3 5 ° C

∑ μ 0 0 0 0 . 0

When pressing

played in the sequence listed on this page;

when pressing , they are displayed in re-

, the parameters are dis-

Can also be set to ‘0’ at any time (via

key).

verse order. The display can be scrolled contin-

uously, i.e. after the last parameter the display

will automatically start again with the first.

If the total section thickness indication is not set to

‘0’ manually, the instrument counts up to maximum

9999.5 μm at which point the counter will automati-

cally switch back to ‘0’.

This indication shows the total thickness of all

sections carried out since the counter was last

set to ‘0’. (All sections, in sectioning and trim-

ming mode, are counted).

1. Refrigeration state

C T - 3 0 ° C

H O M E

O T - 3 5 ° C

ꢀ

ꢀꢀ

Meaning of the refrigeration symbols (asterisks):

--> see chapter 5.3.5 ‘Status indication’.

5. Preset counter

C T - 3 0 ° C

O T - 3 5 ° C

d n 0 5 6

2. Section thickness

C T - 3 0 ° C

O T - 3 5 ° C

μ 0 1 0 . 0

Setting preset counter to ‘0’:

Press

key.

The sectioning thickness is set with the

key (see chapter 5.4.7 ‘Setting the section

thickness’).

can also be pressed while the continuous

sectioning mode is in motion; in this case the

preset counter is set to ‘0’ and sectioning stops

upon reaching the lower limit position.

After it has been set to ‘0’, the preset counter is

inactive.

3. Section counter

C T - 3 0 ° C

O T - 3 5 ° C

∑ n 0 0 0

Reloading the former reference value:

Once displayed (via arrow keys), the section

counter can be set to ‘0’ at any time.

Press

and

simultaneously.

Reloading the former reference value is possible

also while the continuous sectioning mode is in

motion:

Upon pressing both arrow keys, the instrument

continues sectioning without interruption, start-

ing to count down from the old reference value.

•

To set to ‘0’ press

If the section counter is not set to ‘0’ by the user

at some point, sections are counted up to ‘999’

at which point the counter automatically

switches back to ‘0’.

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5. Operation

5.4 Control panel 2

Instruments without sectioning motor

Instruments with sectioning motor

In instruments without sec-

tioning motor, control panel

2 only consists of functional

area B (see below)

In instruments with section-

ing motor, the control panel

is equipped with three func-

tional areas: A, B and C (see

large diagram).

Functional area A:

• Sectioning modes

• Sectioning window

• Sectioning speed

• Emergency stop function

Functional area B:

• Coarse feed

• Trimming

Functional area B:

• Coarse feed

• Trimming

• Section thickness selection

• Start / Stop sectioning

Functional area C:

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5. Operation

Functional areas A and C - Instruments with sectioning motor only

5.4.1 Selecting a sectioning mode /

start and stop sectioning

Start / stop motorized sectioning

Sectioning mode 1 - Intermittent stroke:

Three different sectioning modes can be select-

ed for motorized sectioning:

•

Simultaneously press and hold RUN/STOP and

RUN/ENABLE or depress and hold footswitch.

To stop the sectioning stroke, release the

keys or the footswitch:

Sectioning mode 1 - Intermittent stroke:

The specimen head travels up and

down as long as the RUN/STOP and

RUN/ENABLE keys are pressed simulta-

neously or as long as the footswitch is

depressed.

The specimen head stops immediately.

Sectioning mode 2 - Continuous stroke:

•

Simultaneously press and release RUN/STOP

and RUN/ENABLE or depress and release

footswitch.

Sectioning mode 2 - Continuous stroke:

The specimen head travels up and down

until it is stopped by pressing RUN/STOP

or RUN/ENABLE or by depressing the

footswitch.

•

To stop sectioning:

Press and release RUN/STOP or RUN/EN-

ABLE or depress and release the footswitch:

The specimen head stops upon reaching the

lower limit position.

Sectioning mode 3 - Single stroke:

The specimen head carries out one sec-

tioning stroke and then stops in the low-

er limit position.

Sectioning mode 3 - Single stroke:

•

Simultaneously press and release RUN/STOP

and RUN/ENABLE or depress and release

footswitch.

Activating a sectioning mode

•

The instrument carries out one complete

section / retraction stroke and the specimen

head stops upon reaching the lower limit po-

sition.

Press one of the three sectioning mode keys de-

scribed above. While a sectioning mode is ac-

tive, the LED in the corresponding key will be il-

luminated.

While sectioning is in motion, LED ‘RUN’ is illu-

minated.

While the sectioning motor is deactivated, LED

‘STOP’ is illuminated.

Both LEDs are located in functional area C.

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5. Operation

Functional areas A and C - Instruments with sectioning motor only

The value has been stored when the LED in the

sectioning window key lights up briefly.

5.4.2 Setting the sectioning window

The term ‘Sectioning window’ stands for a dif-

ferent (slower) speed setting in that part of the

sectioning stroke where the specimen is actual-

ly cut, while the remaining sectioning stroke

(above and below the specimen) as well as the

retraction (return) stroke are carried out at a

higher speed level (time-saving feature).

If the LED flashes, the value has not yet been

stored, because the user has tried to set a win-

dow margin outside the possible window range

(the area in which the sectioning window can

be set is slightly smaller than the total section

stroke).

• In that case, repeat the step in question.

If a sectioning window is set, speed inside the

window is as set on the control panel. Outside

the sectioning window the specimen head trav-

els at a proportionately higher speed.

The sectioning window cannot be

deleted. If need be, it can be set to

maximum size (i.e. almost the en-

tire sectioning stroke will be car-

ried out at one and the same

speed).

Always set the sectioning window

while in the sectioning stroke (speci-

men moves downward). Setting the

window in retraction stroke (speci-

men travels upward) is not possible.

Every time after the instrument is

disconnected from mains, the sec-

tioning window is set to maximum

size upon switching back on. This

also applies when the instrument is

switched on for the first time.

•

Position lower edge of specimen approx.

3 mm above the knife edge (rotate hand-

wheel clockwise).

Press sectioning window key.

Position upper edge of specimen just below

the knife edge (continue to rotate the hand-

wheel clockwise).

Press sectioning window key once

more.

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5. Operation

Functional areas A and C - Instruments with sectioning motor only

5.4.4 Emergency stop

5.4.3 Selecting sectioning speed

•

Activate by:

•

Sectioning speed is selected via the sliding

potentiometer. Speed can be changed while

sectioning is in motion.

either pressing the emergency stop

button forcefully (must lock in

position),

•

or depressing the footswitch force-

fully.

Either way, sectioning is interrupted at once.

Caution when working in continuous mode:

If the slider is set to ‘0’, the sectioning motion

stops, however, the actual sectioning mode is

still active - just operating at ‘0’ speed.

In both cases, the red LED in functional

area A lights up, indicating that the emergency

stop function has been activated. Simultaneously

(= all 3 LEDs off).

If the slider is accidentally moved, sectioning

will be resumed! - Risk of injury!

all

sectioning modes are deactivated

Therefore:

•

To deactivate the emergency stop function,

rotate the button in the direction of the ar-

row until it unlocks and slips upward into its

original position.

Do not interrupt sectioning / trim-

ming by setting the sliding potenti-

ometer to zero speed!

As soon as the emergency stop button has been

unlocked, the red LED is extinguished.

See also chapter 2.2 ‘Integrated

safety devices’ incl. subchapter

2.2.4 ‘Knife guard’.

•

Place anti-roll guide onto knife.

Orient specimen, if necessary (see chapter

5.5.5 ‘Specimen orientation’).

•

Select desired section thickness (see chap-

ter 5.4.7 ‘Setting the section thickness’).

•

Start sectioning at approx. 20 μm, reduce

section thickness gradually until reaching

the appropriate thickness for the type of

specimen to be sectioned.

Manual trimming

•

Adjust knife / knife holder relative to speci-

men (see ‘Knife holder’ instruction manual).

•

Discard the first 2 to 3 sections cut at the de-

sired section thickness.

•

Unlock handwheel.

Use motorized coarse feed to bring speci-

men close to knife (see chapter 5.4.5 ‘Coarse

feed functions’).

When sectioning manually, rotate the hand-

wheel evenly and at uniform speed.

•

Select desired section thickness (see chap-

ter 5.4.7 ‘Setting the section thickness’).

For all details on motorized sectioning, please

refer to chapters 5.4.1 to 5.4.4:

•

Move anti-roll plate away from knife.

‘Selecting a sectioning mode / Start and stop

sectioning’, ‘Setting the sectioning window’,

‘Selecting sectioning speed’, ‘Emergency stop’.

Rotate handwheel to trim specimen down to

desired sectioning plane.

Motorized trimming

For advice on appropriate Sectioning tempera-

tures for different types of tissue, please refer to

chapter 6.3 ‘Reference chart for temperature

settings’.

•

Adjust knife / knife holder relative to speci-

men (see ‘Knife holder’ instruction manual).

•

Unlock handwheel.

•

Center handwheel grip (See chapter 2.2.2).

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5. Operation

5.6 Finishing work

5.6.2 Switching off the instrument for a

longer period of time

5.6.1 Daily routine steps

If you do not plan to use the instrument for an

extended period of time, it can be switched off.

Remove all specimens from the cry-

ochamber and store safely! See

also corresponding safety instruc-

tions in chapter 2.3.7 ‘Defrosting /

handling frozen tissue’!

However, since this involves a lot of work (see

below), it should be considered carefully

whether switching off the instrument is really

the better option.

•

Lock handwheel.

If the instrument is switched off, the microtome

must be removed. Then the microtome, cryo-

chamber and all accessories must be disinfect-

ed and dried!

Remove knife from knife holder, disinfect,

dry (see chapter 7 ‘Disinfection, cleaning

and maintenance’) and store in the knife

case.

•

Use a cold brush to sweep frozen sectioning

waste into the section waste tray.

•

For that purpose, follow the instructions in

chapters 7.1 through 7.5.3.

Empty section waste tray (dispose of sec-

tioning waste according to the applicable

regulations on disposal of special category

waste!).

•

If necessary, carry out a complete disinfection

procedure (see chapter

cleaning and maintenance’).

‘Disinfection,

Remove all specimens from the chamber

and store safely.

•

Close sliding window.

Switch off cryochamber illumination.

Use lock/unlock key to lock display in control

panel 1 (Display background dark = locked):

C T . . . . ° C

O T .

. . ° C

1 2 : 0 0

Do not switch off mains switch /

automatic cutout (see chapter 5.2

‘Switching on / fuses’)!

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5. Operation

The specimen head refrigeration is automatical-

ly switched back on when:

5.7 Defrosting

The Leica CM3050 S has 3 (instruments without

specimen cooling: 2) different defrosting options.

•

at least 4 hours have passed since the de-

frost cycle was completed;

•

and the actual chamber temperature devi-

ates less than 5 K from set value.

Automatic chamber defrost cycle

Manual chamber defrost cycle

After the specimen head refrigeration has been

switched back on, the specimen head tempera-

ture rises briefly to +10 °C and then decreases

until it reaches the set value.

Specimen head defrosting (manual) - instru-

ments with specimen cooling only.

Read the safety instructions in chap-

ter 2.3.7 ‘Defrosting / frozen speci-

mens’ !

Switching specimen cooling back on immedi-

ately after defrosting:

As soon as the chamber defrost cycle is com-

pleted, specimen cooling can be switched back

on immediately:

Make sure to remove sensitive speci-

mens from the cryochamber before

the defrost cycle starts!

•

Press

temperature’ is displayed:

until menu item ‘Set specimen

5.7.1 Automatic chamber defrost cycle

S E T

T E M P O T - 3 5 ° C

The automatic defrost cycle is carried out once

every 24 hours at the preselected time.

(For details see chapter 5.3.4 ‘Menu functions’ -

items 6 and 7).

ꢀ -

Press

S E T

T E M P O T - 3 5 ° C

ꢀꢀ

During the defrost cycle, the corresponding as-

terisk is displayed inversely:

Specimen cooling is switched back on.

Despite the possibility of switching

the specimen cooling system back

on immediately after the automatic

chamber defrost cycle, we recom-

mend the automatic defrost cycle

be set at a point in time when the

entire procedure can be controlled

automatically.

C T - 3 0 ° C

H O M E

O T - 3 5 ° C

ꢀ

ꢀꢀ

Once the defrost cycle is finished, the instrument

automatically switches back to refrigeration.

Instruments with specimen cooling:

When defrosting starts, to avoid icing the speci-

men head is automatically adjusted to a temper-

ature between -10 °C and -5 °C, i.e. the set tem-

perature value is deactivated temporarily.

(See also information on menu

items 6 and 7 in chapter 5.3.4 ‘Menu

functions’).

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5. Operation

5.7.2 Manual chamber defrost cycle

5.7.3 Defrosting the specimen head

(instruments with specimen cooling

only)

In addition to the automatic defrost cycle, a

manual chamber defrost cycle can be activat-

ed when necessary.

To activate specimen head defrosting:

•

Press

until menu item ‘Set specimen

•

Press

until menu item ‘Set chamber

temperature’ is displayed:

S E T

T E M P O T - 3 5 ° C

ꢀꢀ

S E T

T E M P

C T - 3 0 ° C

ꢀ -

•

Press

. A brief sound signals that the

Press

. A brief sound signals that the

manual chamber defrost cycle has started:

S E T

T E M P O T - 3 5 ° C

S E T

T E M P

C T - 3 0 ° C

ꢀꢀ

ꢀ -

Specimen head temperature increases to be-

tween +20 °C and +30 °C for a duration of 10

minutes, after which it returns to the set value.

The manual defrost cycle ends either when the

selected defrost cycle duration is over or when

a chamber temperature of -5 °C is reached.

To abort the manual defrost cycle:

To abort specimen head defrosting:

•

Go back to menu item ‘Set chamber tem-

perature’ and press again.

•

Press

until menu item ‘Set specimen

temperature’ is displayed and press

once more.

Instruments with specimen cooling:

When defrosting starts, to avoid icing the spec-

imen head is automatically adjusted to a tem-

perature between -10 °C and -5 °C, i.e. the set

temperature value is deactivated temporarily.

(same as with automatic defrost cycle - see

chapter 5.7.1 ‘Automatic chamber defrost cy-

cle’)

10 seconds after the manual chamber defrost cy-

cle has been completed, the specimen head re-

frigeration is switched back on, cooling down to

the set value.

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6. Troubleshooting, applications tips

6.1 Displayed error messages

4. Service

The autodiagnosis function of the CM3050 S

displays error messages in case of certain mal-

functions.

C T - 3 0 ° C

O T - 3 5 ° C

S E R V I C E

This message is displayed after a

certain number of operating hours

to remind the user to have a preven-

tive maintenance done by Leica

Technical Service.

This message is displayed only, if

the instrument is not regularly ser-

viced by Leica Technical Service.

1. Power failure

This error message is displayed after the instru-

ment had been disconnected from mains, either

due to power failure or because the mains

switch had been turned off.

C T - 3 0 ° C

P O W E R

O T - 3 5 ° C

F A I L U R E

We recommend

contract once the warranty period

has expired!

maintenance

•

To acknowledge the message, press

about 5 seconds.

for

2. Coarse feed

Contact your local Leica sales

office for details!

Coarse feed malfunction has occurred.

C T - 3 0 ° C

? ? ? ?

O T - 3 5 ° C

If one of the error messages listed

under 2, 3 and 4 is displayed, please

contact Leica Technical Service.

This message is also displayed, if the coarse

feed plug has not been reconnected properly

after reinstallation of the microtome.

6.1.1 Other error indications

3. Temperature indications

(red ) in control panel 2 is illuminated:

C T - # # ° C

O T - 3 5 ° C

•

Emergency stop button accidentally activat-

ed.

--> Unlock.

C T - 3 0 ° C

O T - # # ° C

Footswitch not properly connected.

--> Check connection - connect properly,

if applicable.

If the # symbol is displayed instead of chamber

or specimen temperature indication, the corre-

sponding temperature sensor is broken or a

short circuit has occurred.

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6. Troubleshooting, applications tips

6.2 Potential problems - causes and remedies

Problem

Causes

Remedies

Frost on chamber walls and

microtome

- Cryostat is exposed to draft

(open windows, doors, air-

conditioning).

- Eliminate draft sources or

change place of installation of

the cryostat.

- Sliding window has been open

for a long period of time at a

very low chamber temperature.

- Frost build-up caused by

breathing into the cryocham-

ber.

- Close sliding window.

- Consider wearing a mask.

Sections smear

- Specimen not cold enough.

- Knife and/or anti-roll plate not

yet cold enough - sections

melt.

- Select lower temperature.

- Wait until knife and/or anti-roll

plate have reached chamber

temperature.

Sections splinter

Sections not properly flattened

- Specimen too cold.

- Select higher temperature.

- Eliminate cause.

- Static electricity / draft.

- Specimen not cold enough.

- Large surface specimen.

- Select lower temperature.

- Trim specimen parallel; in-

crease section thickness.

- Readjust anti-roll plate.

- Align correctly.

- Anti-roll plate poorly adjusted.

- Anti-roll plate poorly aligned

relative to knife edge.

- Wrong clearance angle

selected.

- Knife blunt or damaged.

- Set correct clearance angle.

- Use different part of knife or

replace knife.

Sections not properly flattened

despite correct temperature

and correctly aligned anti-roll

plate

- Knife and/or anti-roll plate dirty.

- Edge of anti-roll plate dam-

aged.

- Clean with dry cloth or brush.

- Replace anti-roll plate.

- Blunt knife.

- Use different part of knife or

replace knife.

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6. Troubleshooting, applications tips

Problem

Causes

Remedies

Sections curl on anti-roll plate.

- Anti-roll plate does not pro-

trude far enough beyond the

knife edge.

- Readjust correctly.

Scraping noise during section-

ing stroke and specimen return

stroke.

- Anti-roll plate protrudes too far

beyond the knife edge and is

scraping against the specimen.

- Readjust correctly.

Ondulated sections.

- Knife damaged.

- Use different part of the knife

or replace knife.

- Replace anti-roll plate.

- Edge of anti-roll plate damaged.

Chatter marks form during

sectioning.

- Specimen insufficiently frozen

onto specimen disc.

- Refreeze specimen onto disc.

- Check disc clamping.

- Specimen disc not clamped

tightly enough.

- Specimen holder ball joint not

clamped.

- Check ball joint clamping.

- Check knife clamping.

- Knife not clamped tightly

enough.

- Selected section thickness too

thick - specimen has come off

the specimen disc.

- Refreeze specimen onto disc.

- Specimen very hard and inho-

mogeneous.

- Increase section thickness; if

possible, reduce specimen

surface.

- Blunt knife.

- Use different part of knife or

replace knife.

- Knife profile inappropriate for

the type of specimen to be

sectioned.

- Use knife with a different pro-

file.

- Wrong clearance angle.

- Readjust clearance angle set-

ting.

Condensation forms on anti-roll

plate and knife during cleaning.

- Brush, forceps, cloth or other

cleaning item too warm.

- Accessories and cleaning

material to be used pre-cooled

only. - Store on storage shelves

inside the cryochamber.

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6. Troubleshooting, applications tips

Causes

Remedies

Problem

- Anti-roll plate protrudes too far

beyond the knife edge. Adjust-

ment was done in the direction

of the knife.

- Replace anti-roll plate; in

future, lift away from the knife

when aligning.

- Handle anti-roll plate more

carefully.

Anti-roll plate damaged after

adjustment.

- Wrong temperature for the

tissue to be sectioned.

- Select correct temperature.

- Wait until correct temperature

has been reached.

Thick/thin sections.

- Knife profile inappropriate for

the specimen to be sectioned.

- Use knife with different profile

(c or d) or possible switch to

disposable blade system.

- Remove ice.

- Ice build-up on the knife back.

- Handwheel rotation not uni-

form or inappropriate rotation

speed.

- Adapt speed.

- Knife not clamped tightly

enough.

- Specimen disc not clamped

correctly.

- Check knife clamping.

- Check disc clamping.

- Embedding medium poured

onto cold specimen disc; spec-

imen comes off the disc once

frozen.

- Place embedding medium on

luke-warm specimen disc,

inside the cryochamber place

the specimen onto the disc and

freeze in quick-freeze shelf.

- Use different part of knife or

replace knife.

- Blunt knife.

- Incorrect clearance angle.

- Specimen dried out.

- Set correct clearance angle.

- Prepare new specimen.

- Anti-roll plate too warm or

incorrectly adjusted.

- Static electricity.

- Fat on corner or edge of anti-

roll plate.

- Cool or readjust anti-roll plate.

Tissue sticks to the anti-roll

plate.

- Eliminate cause.

- Remove fat (alcohol).

- Knife rusty.

- Remove rust.

- Static electricity or draft.

- Anti-roll plate too warm.

- Eliminate cause.

- Cool anti-roll plate.

Flattened sections curl up as

soon as anti-roll plate is lifted

from the knife.

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6. Troubleshooting, applications tips

Problem

Causes

Remedies

Sections tear.

- Temperature too low for the

tissue to be sectioned.

- Select higher temperature and

wait for the new temperature

to be reached.

- Knife blunt, dirty, dusty, frosted

or rusty.

- Eliminate cause (--> see details

on individual problems).

- Exchange anti-roll plate.

- Upper edge of anti-roll plate

damaged.

- Hard particles in the tissue.

- If application permits, section

at lower plane.

- Clean.

- Rear face of knife dirty.

Inconsistent or insufficient

specimen feed.

- Microtome was not completely

dry when refrigeration was

- Remove microtome from cham-

ber and dry - possibly with hair-

dryer (hairdryer only to be used

outside the cryochamber!) or at

ambient temperature.

switched on; ice build-up inside

the micrometer mechanism.

- Microtome defective.

- Call Technical Service.

Specimen disc cannot be

removed.

- Specimen disc stuck to quick-

freeze shelf or specimen head

due to moisture on underside

of specimen disc.

- Apply strong alcohol to contact

point or heat the specimen

head.

Cryostat inoperational.

- Mains plug not properly con-

nected.

- Check mains plug connection.

- Automatic cut-out has been

triggered.

- Reconnect automatic cut-out

(switch instrument back on).

Sectioning motor inoperational.

- Footswitch not connected.

- Automatic cut-out of sectioning

- Connect footswitch.

- Reconnect automatic cut-out

(push inwards until it locks in

place).

motor has been

gered.

- Motor defective.

trig-

- Call Technical Service.

No or insufficient refrigerating

performance.

- Stopper not placed properly

into drain in bottom of cryo-

chamber.

- Close drain with stopper.

- Compressor defective.

- Leak in cooling system.

- Call Technical Service.

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6. Troubleshooting, applications tips

6.3 Reference chart for temperature settings (in minus °C)

Tissue

10 - 15

15 - 25

25 - 50

Adrenal

ꢀ

Bone marrow

Brain

❄

ꢀ

Bladder

ꢀ

Breast - fatty

Breast - little fat

Cartilage

Cervical

ꢀ

Fatty

ꢀ

Heart and vascular

Intestinal

Kidney

ꢀ

Laryngeal

Lip

ꢀ

Liver

Lung

Lymphoid

Muscular

Nose

ꢀ

Pancreatic

Prostate

ꢀ

Ovarian

Rectal

Skin with fat

Skin without fat

Spleenal or bloody tissue

Testicular

Thyroid

ꢀ

Tongue

Uterus curettage

ꢀ

Please note: The recommended temperature settings are based on long-term experience. However, they can only be

guidelines for general orientation, as each individual specimen may require particular adjustments.

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7. Disinfection, cleaning and maintenance

7.2 Spray disinfection with Leica Cryofect

7.1 Safety instructions

on disinfection and cleaning

For practical spray disinfection, we recommend

Leica Cryofect which is fully effective at tem-

peratures in the negative range down to -20 °C.

When disinfecting the instrument,

make sure to observe the following

safety instructions:

•

Set chamber temperature -20 °C or warmer.

•

Remove specimens from the chamber and

store safely.

•

‘Disinfection and Cleaning’

(Chapter 2.3.10)

•

Carefully remove knife / blade from knife

holder.

‘Handling microtome knives /

blades’

(Chapter 2.3.4)

Remove section waste tray from cryocham-

ber and empty.

•

‘Defrosting / handling frozen

tissue’

(Chapter 2.3.7)

Remove remaining section waste from cryo-

chamber.

Working accessories (brushes, specimen

holders etc.) can be disinfected inside the

cryochamber.

‘Frozen instrument parts and

accessories’

(Chapter 2.3.8)

•

Once the selected chamber temperature has

been reached, spray Leica Cryofect on the

surfaces and accessories to be disinfected.

‘Infectious / radioactive material’

(Chapter 2.3.9)

•

Allow to soak for 15 minutes.

Use paper towels or another type of

disposable cleaning cloths to wipe up

disinfectant and remaining section waste.

•

Dispose of paper towels together with sec-

tion waste.

If previously altered, set chamber tempera-

ture back to its former value.

Put specimens back into the cryochamber.

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Disinfection, cleaning and maintenance

•

Allow cryochamber to dry thoroughly.

Reinstall microtome (see chapter 7.5.3).

Only when they are completely dry, reinsert

the disinfected accessories / tools into the

cryochamber.

7.3 Disinfection with conventional

disinfectants

•

Switch the instrument off.

Carefully remove knife / blade as well as spec-

imens and accessories from the chamber.

Remove section waste from the chamber

and dispose of it.

•

Reinsert the sliding window.

Switch the instrument back on.

•

Once a suitable chamber temperature has

been reached, return the specimens to the

cryochamber.

Remove sliding window (see chapter 7.6 ‘Ex-

changing the fluorescent light lamp’).

Remove microtome (see chapter 7.5.1

‘Removing the microtome’).

Allow the microtome to thaw, disinfect and

allow to dry thoroughly.

7.4 Cleaning

•

When necessary, the instrument surfaces

can be cleaned with mild commercial house-

hold cleaners.

Drying the microtome:

•

Use a hairdryer: dry and before

reinstalling into the chamber

allow the microtome to cool

down to room temperature.

•

Surfaces which are potentially contaminated

(infectious germs) should always be cleaned

with alcohol disinfectants!

Allow to dry at room temperature

(at least over night - longer,

if necessary).

•

Disinfect and dry disassembled parts

(accessories and tools).

Disinfect chamber walls with commercial

alcohol disinfectant.

Place an adequate container underneath the

drain tube at the right side of the instrument.

Pull stopper out of chamber bottom and

drain off disinfecting solution into the con-

tainer.

•

Put stopper back into drain in cryochamber.

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7. Disinfection, cleaning and maintenance

7.5 Removing / reinstalling the microtome

7.5.2 Disassembling the specimen head

(instruments w/specimen cooling only)

7.5.1 Removing the microtome

•

Use Allen key size 5 to remove screw (2)

from the center of the specimen head (1).

When removing the microtome

make sure to observe the following

safety instructions:

•

‘Removing / reinstalling

the microtome’

(Chapter 2.3.11)

•

‘Defrosting / handling frozen

tissue’

(Chapter 2.3.7)

•

Do not twist the refrigerating tube (see

chapter 2.3.11 ‘Removing / reinstalling the

microtome’).

Hold hook (4) (part of standard delivery) in

one of your hands and with your other hand

rest refrigerating tube (3) on hook.

•

‘Infectious / radioactive material’

(Chapter 2.3.9)

‘Disinfection and cleaning’

(Chapter 2.3.10)

Insert hook (4) with refrigerating tube (3) into

the opening above mark (▼).

•

Prior to removing the microtome, carry out

preparation steps as per chapter 7.3 (switch

instrument off, remove accessories etc.)

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7. Disinfection, cleaning and maintenance

•

Remove plug of temperature sensor (7).

Remove plug of coarse feed motor:

7.5.3 Reinstalling the microtome

Pull bushing backward (arrow 1) and hold.

Place your thumb between plug (6) and

socket at the microtome (arrow 2).

To remove plug (6) from microtome, pull it

away from the microtome while at the same

time carrying out a slight jogging movement

When reinstalling the microtome

make sure to observe the following

safety instructions:

•

‘Removing / reinstalling

the microtome’

(Chapter 2.3.11)

(arrows 3).

•

Display message ‘Dry Microtome’

(Chapter 2.3.12)

•

Lock handwheel in lower position.

Place microtome in chamber.

Put one drop of cryostat oil per surface on

the contact surfaces of plastic coupling (11)

and handwheel coupling (12).

Place plastic coupling (11) on handwheel

coupling (12).

Push microtome slightly towards the right

until axle (9) fits tightly into coupling (11).

Unlock handwheel.

Adjust the parts into each other by rotating

the handwheel slightly backward and forth.

Lock handwheel again in lower position.

Tighten screws (8).

Reconnect plugs of coarse feed motor (6)

and temperature sensor (7).

•

Loosen screws (8) with Allen key.

Lift microtome a little and pull slightly to the

left.

•

This loosens the plastic coupling (11) which

connects the two axles.

•

Mount specimen head (in reverse order as

described on previous page).

Insert accessories (storage shelf, section

waste tray etc.).

Insert sliding window.

•

Connect instrument to mains and switch

back on.

•

Remove the microtome from the chamber.

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7. Disinfection, cleaning and maintenance

7.6 Exchanging the fluorescent light lamp

Removal

•

The lamp (3) is located behind the glare

shield (4).

Prior to exchanging the lamp,

please read and observe the fol-

lowing safety instructions:

•

Carefully hold lamp with both of your hands.

Slightly pull the lamp downward at the left

side to remove it from fastening clip (5).

Pull the lamp towards the left to remove it

from its socket (6).

•

‘Maintenance’

(Chapter 2.3.13)

Removing the sliding window

•

Hold sliding window (1) at grip bar (2), lift

slightly and pull out towards yourself.

Installing the replacement lamp

•

Hold the lamp in correct position (see draw-

ing below) and push towards the right until it

locks into the socket.

•

Push the lamp slightly upwards until it locks

into the clip.

•

Put the sliding window back in place.

Reconnect the instrument to mains and

switch it back on.

Leica CM 3050 S – Cryostat

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8. Warranty and service

Warranty

Leica Microsystems Nussloch GmbH guarantees that the contractual prod-

uct delivered has been subjected to a comprehensive quality control

procedure based on the Leica in-house testing standards, and that the prod-

uct is faultless and complies with all technical specifications and/or agreed

characteristics warranted.

The scope of the warranty is based on the content of the concluded agree-

ment. The warranty terms of your Leica sales organization or the organiza-

tion from which you have purchased the contractual product shall apply

exclusively.

Technical service information

If you require technical service or replacement parts, please contact your

Leica sales representative or dealer who sold the product.

Please provide the following information:

•

Model name and serial number of the instrument.

Location of the instrument and name of the person to contact.

Reason for the service call.

Date of delivery.

Decommissioning and disposal

The instrument or parts of the instrument must be disposed of in compliance

with the local laws.

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9. Appendix

1. Electrohydraulic height adjustment

1.3 Safety instructions

An electrohydraulic lifting device for individual

working height adjustment is available as an

optional accessory for the CM3050 S cryostat

series.

Prior to operating the height adjustment device,

make sure to read the present instruction manu-

al. Pay special attention to the following safety

instructions:

1.1 Designated use

Prior to connecting the height ad-

justment device to mains, check to

make sure that the nominal voltage

and frequency indications on the

device name plate correspond to

the actual mains voltage in your

lab!

The electrohydraulic height adjustment device

has been designed exclusively for adjustment

of the instrument working level to the individual

height of the user.

It has been designed for use with the Leica cry-

ostat series CM3050 S only and may under no

circumstances be used to lift other instruments

or persons.

Do not move the height adjustment

device with the Leica CM3050 S in-

stalled on the frame. If the place of

installation needs to be changed,

first take the Leica CM3050 S down

from the height adjustment device

and then move both devices sepa-

rately.

Damage resulting from improper use of the

product and/or faulty operation invalidates the

warranty.

Leica will not assume liability for any such dam-

age.

1.2 Standard delivery

Never place your feet underneath

the U-frame when lowering the

height adjustment device! Risk of

injury!

Electrohydraulic height adjustment device for

cryostat series CM3050 S, complete outfit, con-

sisting of a U-shaped frame on four height-ad-

justable feet and a two-key control unit.

Only authorized Leica technical ser-

vice engineers may carry out re-

pairs on both the Leica CM3050 S

and the height adjustment device.

This applies especially to all work

carried out on the hydraulic system

of the height adjustment device.

The height adjustment device is available in 2

voltages:

1. 230 – 240 V / 50 Hz – order no. 0443 26147

2. 230 – 240 V / 50 Hz – order no. 0443 26147

Leica CM 3050 S – Cryostat

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Appendix

1.4 Installation

•

Open the shipping box and remove all packing material including the Sty-

rofoam pieces.

•

Take the height adjustment device out of the box.

At the place of installation, place the U-frame on the floor with the open

side facing yourself. Adjust the U-frame parallel to the wall, maintaining a

distance of approx. 10 cm between wall and U-frame.

•

Push the (ab) button to lower the height adjustment device to the lowest

possible position.

•

In order to be able to move the instrument,

use a fork wrench and unscrew the rollers

on which the Leica CM3050 S moves by

about 2 cm.

•

Roll the cryostat onto the U-frame as far as it

will go. The rear of the cryostat housing has

to fit closely to the rear bar of the U-frame.

Roll the cryostat onto the U-frame as far as it

will go. The rear of the cryostat housing has

to fit closely to the rear bar of the U-frame.

When placing the instrument onto

the U-frame, make sure the power

cord does not get caught and thus

crushed between U-frame and cry-

ostat housing.

•

Plug the height adjustment device into the

mains socket. The unit is now ready for use.

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9. Appendix

1.5 Control keys

1.7 Troubleshooting

1. Slight and uniform loss of height of the de-

vice in the course of several weeks is nor-

mal. Remedy:

Correct the loss of height by pressing the

button until the instrument is lifted back up to

the desired working level.

2. The cryostat is in an inclined position due to

an uneven upward movement of the lifting

pistons. Difference of level among the four

legs > 5 mm. Remedy:

Have the instrument travel once up and

down over the entire height adjustment dis-

tance: press

until the instrument reach-

until

es the upper limit position and then

the instrument reaches the lowest possible

position. Then lift the instrument back to reg-

ular working height.Should the problem re-

occur several times, call Leica Technical

Service.

The control keys are located on top of the con-

trol unit (see photograph).

•

To lift the instrument, press

3. The height adjustment device does not

reach the maximum upper level or the instru-

ment lowers itself by a relatively large dis-

tance (> 5 cm) within a short time.

To lower the instrument, press

As long as a button is held, the instrument will

go up or down until reaching the upper or lower

limit.

Remedy: Call Leica Technical Service.

1.8 Technical Data

Nominal voltage:

Nomi nal frequency: 50 Hz

230 - 240 V

100 - 115 V

60 Hz

1.6 Maintenance and care

•

Protect the lifting pistons in the four legs of

the height adjustment device against dust

and water.

Type of actuation: Elektrohydraulik

Total height of stroke: 230 mm

Carrying power:

Dimensions:

Width:

200 kp

•

Approximately every six months lubricate the

legs with a thin coat of resin-free oil.

880 mm (without control unit)

1030 mm (with control unit)

555 mm

Once every year have the height adjustment

device checked by an authorized Leica ser-

vice engineer.

Depth:

Weigth:

approx. 30 kg

Leica CM 3050 S – Cryostat

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Appendix

2. Ordering information - accessories

2.1 Knife holders

Knife-holder CN for reusable microtome knives´w/anti-roll guide,

adjustable knife guard and knife support bars for slim and wide knives ............................................. 0419 18649

Knife support bar for knife holder CN, for slim knives .............................................................................. 0419 19426

Knife support bar for knife holder CN, for wide knives ............................................................................ 0419 19427

Knife-holder CE w/ glass anti-roll guide, for low profile disposable blades,

with lateral displacement feature ................................................................................................................ 0419 30383

Knife-holder CE w/ glass anti-roll guide, for high profile disposable blades

with lateral displacement feature ................................................................................................................ 0419 30390

Knife-holder CS for reusable knives, with one knife support

(mounted optionally on the left or right), anti-roll guide and knife guard ............................................. 0419 19787

Auxiliary clamping device for knife holder CS

(to be used instead of knife support, for increased stability) ................................................................. 0419 20971

Knife support bar for knife holder CS .......................................................................................................... 0419 25681

Knife holder base for knife holders CN, CE and CS................................................................................... 0419 26140

Knife rest with section collecting tray for knife holder CS ...................................................................... 0419 18996

2.2 Specimen discs

Specimen disc, 20 mm ø ................................................................................................................................ 0370 08636

Specimen disc, 25 mm ø ................................................................................................................................ 0416 19275

Specimen disc, 30 mm ø ................................................................................................................................ 0370 08587

Specimen disc, 40 mm ø ................................................................................................................................ 0370 08637

Specimen disc, 55 mm ø ................................................................................................................................ 0419 26491

Specimen disc, 50 x 90 mm ............................................................................................................................ 0419 26750

other sizes on request

2.2 Accessories

90° prism ........................................................................................................................................................... 0443 25949

Mobile heat extractor..................................................................................................................................... 0443 26836

Thermoblock .................................................................................................................................................... 0398 18542

Glass anti-roll guide, complete assy., for knife holder CE ....................................................................... 0419 30402

Glass anti-roll guide upgrade kit,

for retrofitting knife holders CN, CS (for C-profile knives) ...................................................................... 0419 30403

2.4 Consumables

OCT embedding compound for freezing tissue specimens onto specimen discs,

bottle of 125 ml ................................................................................................................................................. 0201 08926

Cryostat oil No. 407, bottle of 100 ml ............................................................................................................ 0336 06099

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10. EC Declaration of Conformity

EC Declaration of Conformity

We herewith declare, in exclusive responsibility, that the

Leica CM3050S – Cryostat

was developed, designed and manufactured to conform with the

•

Council Directive 73/23/EEC (Low Voltage),

Council Directive 89/336/EEC, Appendix I (Electromagnetic Compatibility) and

European council Directive 98/79/EG (IVDD).

The following harmonized standards were applied:

•

EN 61010-1: 2001

Safety requirements for electrical equipment for measurement, control and laboratory use -

Part 1: General requirements

EN 61010-2-101: 2002

Safety requirements for electrical equipment for measurement, control and laboratory use -

Part 2-101: Particular requirements for in vitro diagnostic (IVD) medical equipment

EN 61326-1: 1997 + A1: 1998 + A2: 2001 + A3: 2003

Electrical equipment for measurement, control and laboratory use - EMC requirements -

Part 1: General requirements

EN 61000-3-2: 2000

Electromagnetic compatibility (EMC)

Part 3-2: Limits - Limits for harmonic current emissions

EN 61000-3-3: 1995 + A1: 2001

Part 3: Limits -

Section 3: Limitation of voltage fluctuations and flicker in low-voltage

supply systems for equipment with rated current £ 16 A

Electromagnetic compatibility (EMC)

•

EN 418: 1992

Emergency stop equipment.

Principles for design.

•

EN 1037: 1995

Safety of machinery.

Prevention of unexpected start-up.

In addition, the following in-house standards were applied:

DIN EN ISO 9001: 2000.

•

Leica Microsystems Nussloch GmbH

Postfach 1120

D-69222 Nussloch

. . . . . . . . . . . . . . . . . . . . . . . . . . . .

Dag Graupner

Managing Director

January 12, 2004

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11. Decontamination Certificate (master)

Dear Customer,

any product which is be returned to Leica Microsystems or serviced on site, must be cleaned and

decontaminated in the appropriate manner. This certificate, duly completed, must be placed in the unit

prior to packaging or handed over to the service engineer. Packages will not be opend nor servicing

commenced until the Company or service engineer have received a satisfactory certificate. Should

returned goods be considered a hazard by the Company, they will be returned immediately to the

customer at his/her expense.

NB: Microtome knives must be in boxes.

Description

Name/Model

KAT No.

Fabr. No.

Quantity

Tick Box A if applicable. Otherwise please complete all parts of B, providing

further information as requested or appropriate.

Yes

This equipment has not been in contact with unfixed biological samples.

This equipment has been exposed internally or externally to hazardous materials as

indicated below:

Yes No

Please provide further detail here:

Blood, body fluids, pathological samples

Other biohazards

Chemicals/substances hazardous to health

Other hazards

This equipment has been cleaned and decontaminated:

If yes, give details of the method:

Yes No

Please provide further detail here:

If no*, please indicate why not:

* Such equipment must not be returned without the written agreement of Leica Microsystems.

The equipment has been prepared to ensure safe handling/transportation.

Whenever possible, please use the original transportation case/box.

Yes No

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11. Decontamination Certificate (master)

Important - to avoid refusal of shipment:

Place one copy in the unit prior to packaging or hand it over to the service engineer. Customer as-

sumes all responsibility for the immediate return shipment of articles sent to Leica without proper

decontamination documentation.

If you have any further question, please call your local Leica organisation.

Leica Internal Use: If applicable, note corresponding Job and RAN-/RGA-Number:

Job Sheet No.:

____________________

SU Return Goods Authorisation: ____________________

BU Return Authorisation Number: ____________________

Signature/Date

Institute

Department

Adress

Name

Position

Phone

Facsimile

Leica Microsystems Nussloch GmbH

Heidelberger Str. 17-19

69226 Nussloch, Germany

Phone:++49 (0) 6224 143 0

Fax: ++49 (0) 6224 143 200

www.leica-microsystems.com

Leica CM 3050 S – Cryostat

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