Dynex Dishwasher 91000051 User Manual

Opsys MW Microplate Washer  
User’s Guide  
IMPORTANT  
Please read carefully before using the Opsys MW  
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Warranty and Special Provisions Limited Warranty  
DYNEX Technologies products are fully guaranteed for one year against defects in  
parts, materials, and workmanship. Defective parts and materials will be replaced  
or, at the discretion of DYNEX Technologies, repaired at no charge for a period of  
one year and labor required for such replacement or repair will be provided at no  
charge for a period of one year, provided that the products are utilized and  
maintained in accordance with the instructions in the applicable operating and  
servicing manual, and provided further that the products have not, as determined  
solely by DYNEX Technologies, been subject to misuse or abuse by the Customer  
or other parties unrelated to DYNEX Technologies. DYNEX Technologies makes  
no warranty, expressed or implied, as to the fitness of any product for any particular  
purposes other than those purposes described in the applicable operating and  
servicing manual, nor does DYNEX Technologies make any other warranty,  
whether expressed or implied, including merchantability, other than those appearing  
on the face hereof. Where DYNEX Technologies guarantees any product, whether  
under this Warranty or as a matter of law, and there is a breach of such guarantee,  
the Customer’s only and exclusive remedy shall be the replacement or repair of  
defective parts and materials, as described above. This shall be the limit of DYNEX  
Technologies’ liability. Furthermore, DYNEX Technologies shall not be liable for  
incidental or consequential damages. Failure of the Customer to notify DYNEX  
Technologies of a claimed defect by registered mail within thirty days of the  
discovery thereof shall constitute a waiver of any claim for breach of warranty.  
When a product is required by DYNEX Technologies to be installed by a DYNEX  
Technologies engineer or technician, the period of this Warranty shall begin on the  
date of such installation, provided however, that any use of the product prior to such  
installation shall, at sole election of DYNEX Technologies, void this Warranty.  
When installation by DYNEX Technologies personnel is not required, the period of  
this Warranty shall begin on the date of shipment from DYNEX Technologies. The  
period of this Warranty shall begin as described above whether or not the product  
has been installed pursuant to a purchase order, and any trial period shall be  
deducted from the Warranty period that would otherwise apply under a subsequent  
placed purchase order for that product.  
Limitation of Liability. Notwithstanding anything to the contrary contained herein,  
the liability of Seller (whether by reason of breach of warranty, breach of contract,  
tort, or otherwise), including without limitation under any indemnification provision  
contained herein, shall be limited to replacement of goods returned to DYNEX  
Technologies which are shown to DYNEX Technologies’ reasonable satisfaction to  
have been nonconforming or to refund the purchase price, or, if not paid, to a credit  
amount of the purchase price therefore.  
THE FOREGOING WARRANTIES ARE EXCLUSIVE AND ARE GIVEN AND  
ACCEPTED IN LIEU OF ANY AND ALL OTHER WARRANTIES, EXPRESS OR  
IMPLIED, INCLUDING WITHOUT LIMITATION, THE IMPLIED WARRANTY OF  
MECHANTABILITY AND THE IMPLIED WARRANTY OF FITNESS FOR A  
PARTICULAR PURPOSE. NEITHER PARTY SHALL BE LIABLE TO THE OTHER  
FOR ANY INCIDENTAL, INDIRECT, SPECIAL, OR CONSEQUENTIAL  
DAMAGES.  
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About this Manual  
About this Manual  
This manual has been written for laboratory technicians and provides  
detailed instructions for using the Opsys MW™ system.  
This manual gives you the information needed to:  
Review safety precautions.  
Install the Opsys MW™ system.  
Understand the Opsys MW™ menus.  
Set the Opsys MW™ system up to suit your exact needs.  
Create or modify wash protocols.  
Run wash protocols using the Opsys MW™ system.  
Perform required preventive maintenance.  
Troubleshoot the system.  
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About this Manual  
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Description  
Chapter 1 Introduction  
The Opsys MW™ is a microprocessor-controlled microplate washing system  
that performs wash protocols that are defined by the user. It is designed to  
wash all of the wells in one column or one row of a 96-well plate at once.  
The washing protocol can be programmed so that all of the columns (or  
rows) are washed in the same manner, or different wash cycles can be  
applied to specified columns (or rows) on the plate.  
Description  
Figure 1. The Opsys MW™ Microplate Washing System  
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Description  
Programming  
All wash protocols on the Opsys MW™ Microplate Washer are user-defined.  
Up to 40 wash protocols can be contained on the system.  
Note: Memory limitations may restrict the size  
and complexity of an individual wash protocol.  
In addition, the system can be configured with up to ten different plate types  
so that the wash head positions for each plate type can be specified.  
Programming of a new wash protocol (or modifying an existing one) and  
configuration of the system are carried out using the keypad and display.  
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Description  
Wash Head  
The wash head on the Opsys MW™ Microplate Washer contains multiple  
sets of dual wash pins. Each set of dual wash pins consists of a  
dispense pin and an aspirate pin that are closely spaced so that fluid can  
be aspirated from and dispensed into a well simultaneously.  
The following wash heads can be fitted on the Opsys MW™:  
Wash Head  
1 x 8  
Description  
Eight sets of dual wash pins for washing a 96-well  
microplate one column (8 wells) at a time.  
1 x 12  
Twelve sets of dual wash pins for washing a  
96-well microplate one row (12 wells) at a time.  
If a wash head is installed to wash plates by columns, the plates are placed  
on the instrument so that the columns of the plate are aligned with the wash  
head. If a wash head is installed to wash plates by row, the plate is rotated  
90 degrees and placed on the instrument so that the rows of the plate are  
aligned with the wash head.  
The wash pins are fixed to the wash head. During operation, the wash head  
assembly is lowered to insert the wash pins into the wells or raised to  
remove the wash pins from the wells. Lowering the wash head assembly  
allows the well contents to be aspirated or a bottom wash to be performed.  
Raising the wash head assembly allows the plate to be moved so another  
column (or row) can be washed or so the wells can be filled.  
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Description  
Wash Head Positions  
The vertical positions that the wash head can assume are described below.  
Each wash head position can be specified by the user to within 0.1 mm.  
Note: Procedures for specifying wash head  
positions for various plate types are described  
Wash Head Position  
Description  
Dispense Height  
Positions the aspiration pins slightly  
below the top of the well so that fluid can  
be dispensed into the well up to a certain  
level before it is aspirated.  
Well Top  
Positions the aspiration pins so they are  
aligned with the top of the well.  
Aspiration Height  
Positions the aspiration pins at the bottom  
of the well so that the contents of the well  
can be completely aspirated.  
Sweep Height  
Raises the aspiration pins slightly above  
the Aspiration Height (see above) so that  
the aspiration pins can be moved back  
and forth in the well while the fluid is  
being aspirated.  
Bottom Wash Height  
In protocols with a bottom wash, lowers  
the wash head during dispense so that  
the force of the dispensed fluid can wash  
the bottom of the wells.  
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Description  
Wash Fluid  
A single wash fluid is used on the Opsys MW™ Microplate Washer. The  
wash fluid is contained in the Wash Container and is dispensed into the  
wells whenever a Dispense or Fill operation is specified in the wash  
protocol. In addition, wash fluid is dispensed into the purge tray during a  
Purge operation.  
Dispensing of the fluid from the Wash Container is controlled by a pump  
attached to the back of the Opsys MW™ Microplate Washer and a dispense  
valve located near the wash head (Figure 1).  
Waste  
Fluid is removed from the wells during an Aspirate or Dispense operation  
and collected in the Waste Container. The Waste Container contains a level  
sensor to alert the operator when the container is full.  
Removal of the fluid from wells is controlled by lowering the wash head so  
that the aspiration pins are immersed into the well fluid (the aspiration  
height). A vacuum is applied to the aspiration pins at all times during  
operation so that the contents of a well are aspirated whenever the wash  
head is lowered to the aspiration height.  
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Description  
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Features  
Features  
The Opsys MW™ system has a number of performance and convenience  
features. These are summarised below:  
Small footprint  
Up to 40 wash protocols can be programmed  
Up to ten plate types can be programmed  
Two wash head configurations (1 x 8 and 1 x 12)  
On-board self diagnostics  
SHOW function allows visual verification of wash head height  
settings  
High-level alarm on Waste Container  
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Features  
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Specifications  
Specifications  
Dimensions and Weight  
Length  
Width  
49 cm (19.3 in)  
38 cm (15.0 in)  
19 cm (7.5 in)  
9.1 kg (20 lbs)  
Height  
Weight  
Operation  
Dispense Range  
50 – 999 µL  
Dispense Precision  
Residual Aspirate Volumes  
5% CV (with 300 µL distilled water)  
< 1 µL for U and V bottom plates  
< 5 µL for flat bottom plates  
Washing Time  
Power  
Typical 5 cycle wash: 5 min 30 sec  
< 100 W  
Display  
2 by 20 character back lit LCD  
21 key tactile membrane  
Display, Printer, RS232  
Keypad  
Output  
Environmental  
Operating Range  
15° C to 30° C  
15% to 85% relative humidity (non-condensing)  
2000m altitude  
Computer Interface  
Port  
Serial RS-232 port (Service Access)  
19200. Character format  
Baud Rate  
Character Format  
7 data bits, 1 stop bit, no parity  
Power Requirements  
Voltage  
Power  
Frequency  
50/60 Hz  
Main Unit  
100 - 240 V  
200 VA  
Line Voltage Variation  
±
10%  
3 Hz  
Line Frequency Variation  
±
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Specifications  
Input Ranges  
Assay Programming  
Assay Name  
Up to 10 characters  
Password  
Up to 8 characters  
Move: Start Strip  
Move: End Strip  
Move: #Cycles  
Aspirate: Cycles  
Dispense  
1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1)  
1 - 8 for 1 x 8 heads and 1 - 12 for 1 x 12 heads (Increment: 1)  
1 - 9 (Increment: 1)  
1 - 9 (Increment: 1)  
50 - 999 (Increment: 1 µL)  
Purge  
50 - 9999 (Increment: 1 µL)  
Fill  
50 - 300 (Increment: 1 µL)  
Soak  
1 - 999 (Increment: 1 second)  
Plate Setup  
Plate Name  
Up to 10 characters  
Dispense Height  
Top of Well Height  
Aspirate Height  
Sweep Height  
001 - 999 (Increment: 1)  
001 - 999 (Increment: 1)  
001 - 999 (Increment: 1)  
001 - 999 (Increment: 1)  
001 - 999 (Increment: 1)  
001 - 999 (Increment: 1)  
Sweep Stroke  
Bottom Wash: Dispense  
Height  
Standards  
The instrument is designed in accordance with CSA 1010.1-92, UL 3101-1, EN61010-1  
and EN61326.  
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Warning Labels  
Warning Labels  
The Opsys MW™ or its components may contain certain labels that that  
either warn the user of a hazard or note an electrical connection. The labels  
that may be used on the Opsys MW™ system are described below.  
Label  
Description  
Alternating current is present.  
(English) Caution symbol. Refer to the  
Routine Maintenance chapter.  
(French) Attention (voir documents  
d’accompagnement).  
(English) Caution, hot surface.  
(French) Attention, surface chaude.  
Protective conductor terminal.  
Earth (ground) terminal.  
(English) Caution, risk of electric shock.  
(French) Attention, risque de choc électrique.  
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Warning Labels  
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Unpacking  
Chapter 2 Installation  
Unpacking  
Materials Provided  
The main carton contains the instrument. The contents of this carton are  
listed below:  
Article  
Quantity  
Opsys MW™ System  
Instrument Power Cable  
Cleaning Wire, Aspirate  
Cleaning Wire, Dispense  
Instrument Configuration Report  
User’s Guide  
1
1
1
1
1
1
1
1
1
Declaration of Conformity  
Wash Head, 1 x 8 or 1 x12  
Plate Holder  
A sub-carton contains the wash container and waste container assemblies.  
The contents of this carton are listed below:  
Article  
Quantity  
Wash Container Assembly  
Waste Container Assembly  
1
1
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Unpacking  
To unpack the components:  
1. Place the Opsys MW™ instrument in the position where it will be  
located for use.  
2. Place the Wash Container, Waste Container and Foam/Aerosol  
Trap at the rear of the instrument.  
3. Examine the packaging to be sure that the power cord and other  
materials have been removed. Please save packaging material  
for future use.  
4. Verify that all of the materials listed above have been unpacked.  
5. Inspect the components for damage. If damage is observed,  
contact your shipper or service representative immediately.  
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Hardware Components  
Hardware Components  
Hardware components of the Opsys MW™ instrument are shown in  
Note: The Wash Container, Waste Container  
and Foam/Aerosol Trap are external to the  
Wash Head  
Microtiter® Plate Plate Carrier Display Panel Keypad  
Figure 2. The Opsys MW™ System  
Wash Head. The wash head contains sets (1 x 8 or 1 x 12) of dual  
wash pins. Each set of wash pins consists of a dispense pin and  
an aspirate pin, closely spaced so that fluid can be aspirated from a  
well while fluid is being dispensed into the same well. The wash  
head moves back and forth to wash each row (or column, depending  
on the wash head that is used) of the plate by lowering the wash  
pins into the corresponding wells.  
Plate Carrier. The plate carrier holds the plate in a fixed position so  
that the wash pins can be precisely lowered to the correct height and  
position in the wells during the washing procedure.  
Display Panel. The display panel is the means by which the system  
communicates to the user. All messages, commands, and wash  
parameters entered by the user are displayed.  
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Hardware Components  
Keypad. The keypad is used by the operator to select commands  
and enter wash parameters.  
RS232 Interface. The system includes an RS232 interface so that  
an external computer can be connected for technical service  
purposes.  
the wash fluid that is used for Dispense, Fill and Purge operations.  
contains the fluid that is aspirated from the wells. A level sensor is  
contained inside of the waste container.  
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Hardware Components  
Connectors at the rear of the Opsys MW™ system are shown in Figure 3.  
Connections for the power cord, dispense pump power, waste bottle level  
sensor, waste bottle vacuum line and an external computer are made at the  
rear of the system.  
Important: Be sure that the vacuum exhaust  
is not obstructed.  
Figure 3. Rear View of the Opsys MW™ System  
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Positioning the Instrument  
Positioning the Instrument  
Determine the area where the system will be located. You will need a firm  
and level area that is approximately 51 cm (20 inches) wide, 62 cm (24  
inches) deep, and 40 cm (16 inches) high for the Opsys MW™.  
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Installing the Wash Head  
Installing the Wash Head  
The Wash Head must be located on the Wash Head Arm.  
To install the Wash Head:  
1. Position the Arm over the Purge Reservoir portion of the Plate  
Holder.  
2. Place the Wash Head on the two Arm Pins, being careful to  
properly seat the Wash Head on the support pins.  
3. Level the Wash Head in relation to the Plate Holder and tighten  
the two thumbscrews at the front of the Wash Head / Arm  
Assembly.  
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Installing the Wash Container  
Installing the Wash Container  
The dispense tubing and dispense pump power cable must be connected.  
To install the Wash Container:  
1. Fill the Wash Container with the wash solution that is to be used.  
Note: The Wash Container contains up to  
4 liters.  
2. Insert the Pick-Up Filter into the Wash Container and secure the  
cap. Attach the Quick-Connector at the end of the pump tubing,  
to the mating connector in the cap.  
3. Route the dispense tubing through the upper slot at the front  
corner of the instrument and through the routing clips at the front  
of the instrument.  
4. Connect the end of the dispense tubing to the wash tubing  
connector on the wash head.  
5. Thread the dispense tubing through the dispense valve.  
Note: The tubing must be inserted fully into  
the dispense valve opening to ensure proper  
operation.  
6. Connect the dispense pump power cable to the dispense pump  
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Installing the Waste Container  
Installing the Waste Container  
To install the Waste Container and Foam/Aerosol Trap:  
1. Insert the Quick-Connect end of the vacuum tubing on the  
Foam/Aerosol Trap to the Quick-Connect vacuum connector at  
2. Screw on the Waste Container cap.  
3. Route the waste tubing through the lower slot at the front corner  
of the instrument and through the routing clips at the front of the  
instrument.  
4. Connect the end of the waste tubing to the waste tubing  
connector on the wash head.  
5. Connect the waste level sensor cable to the waste level sensor  
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Turning on the System  
Turning on the System  
Note: Depending upon local electrical codes,  
an uninterruptible power system (UPS) may be  
required in your laboratory. A UPS is not  
provided with the Opsys MW™ system.  
1. Connect the Opsys MW™ to the laboratory electrical supply  
outlet.  
CAUTION: The Opsys MW™ instrument must  
be connected to properly grounded electrical  
outlets. Obtain assistance from a qualified  
electrician to verify that your electrical outlets  
are properly grounded.  
Before connecting the power cable, be sure  
that the components have been connected to  
each other as outlined in the previous section.  
2. Push the instrument power switch once. After a series of self-  
tests, the software version is displayed momentarily  
Opsys MW. v1.0  
before the main menu is displayed:  
1: - - - - - - - -  
SETUP PROGRAM  
Note: The self-test results indicate whether  
the system is operating properly. If a self-test  
failure is reported, it is recommended that you  
call Technical Service.  
Dashes are initially displayed after the wash  
protocol number (i.e., 1: - - - - - - - - in the  
above example) if a wash protocol has not yet  
been named.  
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The Keypad  
Chapter 3 Configuration  
The Keypad  
The keypad is used to access the menus and commands for setting up the  
Opsys MW™ entering new wash protocols and running the wash protocols.  
The keypad contains scroll keys, function keys, and alphanumeric keys.  
Scroll Keys  
Display Panel Function Keys  
Scroll Keys  
Alphanumeric  
Keys  
3
1
2
ENTER  
START  
DEL  
DEF  
GHI  
ABC  
6
5
4
+-*/=  
JKL  
PQR  
MNO  
8
9
0
7
CANCEL  
YZ  
<>_()  
STU  
VWX  
Figure 4. Keys on the Keypad  
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The Keypad  
The use of each of the keys is summarized below:  
Key  
Purpose  
Scroll keys  
The functionality of the scroll keys depends upon  
what is being displayed.  
pressing the up or down scroll keys will display  
successive wash protocol numbers and names (or  
dashes, if a protocol is not yet named).  
Horizontal scrolls will scroll through selections for  
the selected menu. Repeatedly pressing the  
horizontal scroll button will show the next two  
items in a list. A list with one item will have a  
blank entry.  
If you are entering or editing information, a  
scroll will move the cursor through edit fields.  
When editing an existing plate or protocol name,  
you may either type over the existing name, or  
press the DEL key to delete the characters from  
the existing name. (NOTE:To delete the entire  
name, either type over it or press the right arrow  
key and DEL to delete all characters to the right of  
the cursor. The first character of an existing name  
cannot be deleted, but it can be overwritten.)  
Function keys  
“Del” key  
Two function keys select the item or execute the  
command shown directly above each key on the  
second line of the display.  
When a modifiable entry is displayed, selecting  
Delete will allow the entry to be modified. While  
editing a field, selecting Delete removes the  
character at the cursor location and all characters  
to the right of the cursor.  
“Enter” key  
Completes an entry of characters, accepts default  
prompt data, and displays the next prompt in a  
series of commands  
“Start” key  
Starts a wash protocol. This can only be done  
while the main menu is being displayed.  
“Cancel” key  
Returns to the previous menu within the function  
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The Keypad  
Key  
Purpose  
The numeral shown on the key is displayed by  
pressing the key once. If the key is pressed two to  
seven times rapidly, one of the letters on the key  
will be displayed. The letter that is displayed  
depends upon the number of times the key is  
pressed.  
Alphanumeric  
Keys  
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Menus  
Menus  
The Opsys MW™ menus are hierarchical and are organised by function.  
There are two main menus: Setup and Program.  
Menu  
Purpose  
SETUP  
Configure the Instrument  
PROGRAM  
Create or edit plate washing protocols *  
* A protocol includes general information (e.g. the type of microplate to be  
washed) as well as a listing of the various steps to be used in the washing  
process.  
Once the instrument is switched on and the self-test is successfully  
completed, the main menu is displayed. It contains SETUP and PROGRAM  
menu headers:  
1: --------  
SETUP  
PROGRAM  
Note: Dashes are initially displayed after the  
wash protocol number (i.e., 1: - - - - - - - - in  
the above example) if a wash protocol has not  
yet been named. Once a wash protocol is  
named, the name is displayed after the  
protocol number. Refer to page 41 for the  
procedure to create and name a wash  
protocol.  
Display a different wash protocol number and name (there can be up to 40)  
by pressing the up or down scroll keys or by entering the protocol number  
using the keypad.  
Access a menu by pressing the function key beneath the menu header.  
Cancel a menu selection and display the previous (higher level) menu by  
pressing the CANCEL key.  
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Menus  
The SETUP Menu  
Press the function key under SETUP to display the SETUP menu  
1: TESTNAME  
SETUP  
PROGRAM  
Select Option  
HEAD  
PLATE  
CAL.  
Select Option  
VERSION  
Figure 5. SETUP Menu  
The PROGRAM Menu  
Press the function key under PROGRAM to display the PROGRAM menu  
new wash protocol or to edit an existing wash protocol.  
1:TESTNAME  
SETUP  
PROGRAM  
Enter name  
– – – – – – – – –  
(If selected protocol has  
not been named)  
or:  
(If selected protocol has  
been named and has no  
password)  
Edit name  
4: WASHNAME  
or:  
(If selected protocol has  
been named and has a  
password)  
Enter password  
Figure 6. PROGRAM Menu  
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Configuring the System  
Configuring the System  
The Opsys MW™ must be configured before it is operated for the first time.  
Use ARROW keys to scroll through the menus.  
1: TESTNAME  
SETUP  
PROGRAM  
Select Option  
HEAD  
PLATE  
CAL.  
Select Option  
VERSION  
Figure 7. SETUP Menu  
The purpose of each command on the SETUP menu is summarised below:  
Command  
Purpose  
HEAD  
Specify the type of head that is fitted  
PLATE  
Displays the PLATE menu. The commands on this  
menu allow parameters (such as well top height,  
dispense height and wash height) for various plate  
types to be specified.  
VERSION  
CAL.  
Displays the version number of the software  
Displays the CAL. menu. The commands on this  
menu allow the pump parameters to be specified.  
CLEAN  
The CLEAN command enables the washer to be  
cleaned automatically at a specified interval. This  
feature assists in assuring that tubing and dispense  
pins are free from potential salt build up.  
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Configuring the System  
Specifying the Wash Head  
The HEAD command allows the installed wash head to be specified:  
1. Select SETUP. The display will read:  
Select option  
HEAD  
PLATE ꢀ  
2. Select HEAD. The current wash head selection is displayed in  
the upper line:  
Wash head?  
8
8
If needed, scroll right to view the remaining selection:  
Wash head?  
12  
8
3. Select the desired option using the function key. After the  
selection is made, the display will again read:  
Select option  
HEAD  
PLATE ꢀ  
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Configuring the System  
Specifying Plate Parameters  
The PLATE command allows plate parameters to be specified for up to 10  
plate types.  
When setting plate parameters, a default value is displayed for each of the  
wash head positions. The default values are factory-set and represent  
approximate starting values for each wash head position.  
Note: The wash head positions are described  
Increasing the value of a wash head position by one unit lowers the head  
0.1 mm and decreasing the numerical value of a wash head position by one  
unit raises the head 0.1 mm. (e.g. an increment of 20 steps equals one  
millimeter of vertical pin travel (+20 = 1mm up, -20 = 1mm down)).  
The SHOW command is available when setting each wash head position.  
Select SHOW to move the wash head to the indicated position so that the  
height can be observed.  
For example, when setting dispense height, the default value is 180:  
DISP. HEIGHT? 180  
SHOW  
If, after selecting SHOW, the dispense pins are found to be too low by  
approximately 1.0 mm, the dispense height position can be raised by 1.0  
mm by entering 170 for the dispense height.  
When using the SHOW function when defining the aspirate height, if the  
aspirate pins touch the well bottom, increase the aspirate height. If you  
cannot move the plate slightly side-to-side and up-down without immediate  
interference from the aspirate pins, increase the aspirate height.  
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Configuring the System  
To specify parameters for a plate type:  
1. Obtain an empty plate of the plate type being specified and place  
it in the plate holder.  
2. Select SETUP (if needed). The display will read:  
Select option  
HEAD  
PLATE ꢀ  
3. Select PLATE. The name of the first plate is displayed in the  
lower line:  
Select plate  
1: - - - - - - - - - -  
Note: Dashes will be displayed if the plate has  
not yet been named.  
4. If needed, display a different plate number using the up or down  
scroll keys (or use the keypad to enter the desired plate number).  
When the desired plate number is displayed, press ENTER. The  
display will read:  
Enter name  
- - - - - - - - - -  
5. Use the keypad to enter the plate name (up to  
10 characters).  
Note: Refer to page 23 for instructions on  
entering alphabetic characters.  
6. Press ENTER. The display will read:  
DISP. HEIGHT? 180  
SHOW  
Note: The default value is displayed. Select  
SHOW to move the wash head to the default  
dispense position so that the height can be  
checked. Then, determine the correct value as  
needed.  
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Configuring the System  
7. Enter the dispense height using the keypad and press ENTER.  
The display will read:  
Well top? 160  
SHOW  
Note: The default value is displayed. Select  
SHOW to move the wash head to the default  
well top position so that the height can be  
checked. Then, determine the correct value as  
needed.  
8. Enter the desired value using the keypad and press ENTER. The  
display will read:  
ASP. HEIGHT? 380  
SHOW  
Note: The default value is displayed. Select  
SHOW to move the wash head to the default  
aspiration position so that the height can be  
checked. Then, determine the correct value as  
needed.  
9. Enter the desired value using the keypad and press ENTER. The  
display will read:  
Sweep?  
YES  
NO  
NO  
10. Select YES to specify a sweep. The display will read:  
All cycles?  
YES  
NO  
NO  
Note: If a sweep is specified, the wash pins  
are moved up and down and across the  
bottom of the well during aspiration to better  
remove all droplets of liquid. If NO is selected,  
the Wash height? prompt (Step 14) will be  
displayed.  
Note: Use the sweep function with Flat or “C”  
bottom plates only. Sweeping aspirates fluid  
more completely from the wells than a regular  
aspiration. Sweep all cycles to get the most  
thorough well aspiration when using multiple  
aspiration cycles, but remember that each  
sweep adds seconds to the protocol.  
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Configuring the System  
Remember if your protocol requires sweeping,  
that must first be specified in the plate  
definition used in the protocol.  
11. Select YES to specify a sweep on all aspiration cycles. The  
display will read:  
Sweep height? 370  
SHOW  
Note: The default value is displayed. Select  
SHOW to move the wash head to the default  
sweep position so that the height can be  
checked. Then, determine the correct value as  
needed.  
12. Enter the desired value using the keypad and press ENTER. The  
display will read:  
Sweep stroke? 100  
Note: There isn’t a SHOW function for the  
sweep stroke. Determine optimum sweep  
stroke setting by starting with a small number  
and increase it if the residual aspirate volume  
is not acceptable. If you run a protocol and  
the wash pins strike the sides or bottom of the  
well, reduce the sweep stroke and review your  
plate setup parameters.  
13. Enter the desired value using the keypad and press ENTER. The  
display will read:  
Bottom wash?  
YES  
NO  
NO  
14. Select YES if a bottom wash is to be included. The display will  
read:  
Wash height? 368  
SHOW  
Note: The default value for bottom wash  
height is displayed. Select SHOW to move the  
wash head to the default bottom wash position  
so that the height can be checked. Then,  
determine the correct value as outlined on  
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Configuring the System  
15. Enter the desired value using the keypad and press ENTER. The  
display will read:  
Select option  
HEAD  
PLATE ꢀ  
Note: If NO is selected for Bottom wash?  
(Step 13), the display will read as shown  
above.  
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Configuring the System  
Displaying the Software Version  
The VERSION command displays the software version that is currently  
installed on the Opsys MW™ instrument:  
1. Select SETUP (if needed). The display will read:  
Select option  
HEAD  
PLATE ꢀ  
2. Scroll right to display the next selection:  
Select option  
VERSION  
3. Select VERSION. The display will momentarily read:  
Opsys MW. v1.0  
CAL. ꢀ  
Note: The software version that is displayed  
will depend on the version installed in the  
instrument.  
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Configuring the System  
Specifying the Pump Parameters  
The CAL. command allows the calibration values for each pump to be  
specified:  
1. Select SETUP (if needed). The display will read:  
Select option  
HEAD  
PLATE ꢀ  
2. Scroll right to display the next selection:  
Select option  
VERSION  
3. Select the pump pressure (high or low). The display will read:  
Pump speed? 190  
CAL. ꢀ  
4. Enter the desired value using the keypad and press ENTER. The  
display will read:  
Slope?  
2.1  
Note: The Slope and Intercept values should  
only be changed by DYNEX Technologies  
Service Personnel.  
5. Press ENTER to keep the existing value. The display will read:  
Intercept? 24  
6. Press ENTER to keep the existing value. The display will again  
read:  
Select option  
VERSION  
CAL. ꢀ  
Note: Refer to page 74 for additional pump  
calibration instructions.  
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Configuring the System  
Selecting Clean  
The CLEAN command enables the washer to be cleaned automatically at a  
specified interval.  
1. Scroll right to display the next section:  
Select option  
CLEAN  
2. Select CLEAN. The display will read:  
Auto Clean?  
YES  
NO  
3. Select YES if you want periodic automatic cleaning. The display will  
read:  
Interval (Hrs)  
1
4. Enter the desired interval using the up/down scroll keys and press  
ENTER.  
Note: The minimum interval is 1 hour, and the  
maximum interval is 9 hours.  
The display will read:  
Fluid Volume?  
2500  
5. Enter the desired volume of fluid to be dispensed.  
Note: A cleaning volume ranging from 50 to  
9,999 microliters can be entered. Follow the  
instructions on page Error! Bookmark not  
6. Press ENTER. The display will read:  
Select option  
CLEAN  
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Configuring the System  
This page is intentionally left blank  
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Wash Protocol Operations  
Chapter 4 Wash Protocols  
Wash Protocol Operations  
A wash protocol consists of a series of Purge, Move, Soak, Aspirate,  
Dispense and Fill operations.  
Purge, Move and Soak can be carried out in any sequence. Aspirate,  
Dispense and Fill can only be carried out within a Move operation, and  
there cannot be a Move within a Move.  
Each of these operations is described below:  
Operation  
Purge  
Description  
Dispenses fluid from the dispense wash pins while  
the wash head is positioned over the purge tray  
Note: Purge is usually  
carried out at the beginning  
of a wash protocol to rinse  
the dispense wash pins and  
remove air bubbles.  
Move  
Performs Aspirate, Dispense, Fill and/or Soak  
operations on specified strips of the Microplate  
Removes the contents of a well by positioning the  
wash pins at the aspiration height in the well and  
aspirating the liquid from the wells  
Aspirate  
A sweep may also be performed during Aspirate.  
Note: The aspirate height  
and sweep parameters for a  
particular plate type are  
specified during system  
If a sweep is specified during  
configuration, the wash pins  
are moved back and forth  
during the aspiration cycle.  
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Wash Protocol Operations  
Operation  
Dispense  
Description  
Dispenses a specified amount of fluid into the wells  
after aspirating the contents of the wells (i.e.  
wherever a DISPENSE step is added to a protocol,  
the OPSYS MW will automatically aspirate the  
wells before dispensing more fluid in order to  
prevent a well overflow). If a bottom wash is  
specified, the wash head is then lowered to the  
bottom wash position so that fluid will be aspirated  
from the bottom of the wells while fluid is being  
dispensed.  
Note: The dispense height  
for a particular plate type is  
specified during system  
The contents of the wells are allowed to equilibrate  
for the specified number of seconds.  
Soak  
Fill  
The wells are filled with a specified amount of fluid.  
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Creating a New Wash Protocol  
Creating a New Wash Protocol  
Specifying the Header  
NOTE: Pressing CANCEL while creating or  
editing a protocol will bring you back to the  
main menu without saving your changes.  
or ꢃ  
Select one of the 40 protocol numbers.  
(or use keypad)  
PROGRAM  
Select PROGRAM from the main menu.  
Enter a protocol name (up to 9 characters).  
Enter an optional password.  
(keypad)  
(keypad)  
NOTE: If the password for a protocol is lost, the ability  
to access the method and the method position are also  
lost.  
or ꢃ  
Select the plate type.  
(or use keypad)  
Specify whether the operator will be prompted at run  
time to enter the number of strips to be washed (avoids  
reprogramming the number of strips).  
Variable strips?  
(If Variable strips? is YES). Specify whether the time  
for washing any number of strips on a plate is constant.  
Constant timing?  
Figure 8. Specifying the Wash Protocol Header  
After the wash protocol header information is defined, the specific steps in  
the wash protocol are specified.  
The procedures for defining the wash protocol header are described in the  
following pages. The procedures for specifying the steps in a wash protocol  
are described beginning on page 45.  
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Creating a New Wash Protocol  
To create a new wash protocol header:  
1. Display the main menu.  
1: - - - - - - - - - -  
SETUP  
PROGRAM  
2. Use the up or down scroll keys to display the protocol number (or  
use the keypad to enter the protocol number) that is to be  
created:  
6: - - - - - - - - - -  
SETUP  
PROGRAM  
Note: Dashes will be displayed if a protocol  
has not yet been named.  
3. Select PROGRAM. The name of the wash protocol is displayed  
in the lower line:  
Enter name  
- - - - - - - - - -  
4. Use the keypad to enter the protocol name (up to 9 characters)  
and press ENTER. The display will read:  
Password?  
YES  
NO  
Note: Refer to page 24 for instructions on  
entering alphanumeric characters.  
5. Select YES if the protocol is to be password protected. The  
display will read:  
Enter password  
********  
Note: If NO is selected, the Select plate  
prompt (Step 7) will be displayed.  
6. Enter the password, (up to eight numeric characters) and press  
ENTER. The display will read:  
Confirm password  
********  
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Creating a New Wash Protocol  
7. Re-enter the password and press ENTER. The display will read:  
Select plate  
2: DYNEX_1  
Note: The plate name that is shown is an  
example. The actual name will be the plate  
name that is entered for that plate type during  
8. Use the up and down scroll keys to select the plate type (or enter  
the number using the keypad) to be used and press ENTER. The  
display will read:  
Variable strips?  
YES  
NO  
Note: “Variable Strips” is used to run an  
established protocol on fewer strips than  
originally specified in the “MOVE” cycle(s) of  
an established protocol.  
If YES is selected, the operator will be  
prompted at run time to specify the number of  
strips that are to be washed. Processing will  
begin on the first strip selected in the “MOVE”  
cycle and will end on the total number of strips  
entered at run time (e.g. the protocol states:  
“Move 3-12 1 cycle(s)” and at run time, 2  
strips are entered, only strips 3 and 4 will be  
processed).  
9. If YES is selected, the display will read:  
Constant timing?  
YES  
NO  
Note: If NO is selected for Variable strips?,  
the Select option prompt will be displayed  
(see below).  
Note: The Constant Timing prompt only  
appears if you are using Variable Strips.  
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Creating a New Wash Protocol  
10. Select YES if the total elapsed time for washing any number of  
strips within a plate is to be constant. The display will read:  
Select option  
MOVE  
PURGE ꢀ  
The sequential steps in the wash protocol can now be specified,  
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Creating a New Wash Protocol  
Specifying Wash Cycle Operations  
The final step in creating a wash protocol is to specify individual operations  
within the wash protocol.  
dispense  
pin  
wash  
s are purged, and then a Dispense and Aspirate are carried out  
twice on each of the 12 strips (columns) of a 96-well Microtiter® plate.  
Finally, after all 12 strips have been washed twice, the contents of the  
Microtiter plate are allowed to soak (equilibrate) for another 600 seconds.  
PURGE  
Purge the dispense pins.  
MOVE  
(2 Cycles)  
Specify the starting strip and ending strip for each cycle  
and the number of cycles to be performed.  
DISPENSE  
(3 Times)  
For each strip, perform dispense three times.  
For each strip, perform one aspiration cycle.  
End the cycle.  
ASPIRATE  
END LOOP  
After the two Move cycles have been completed, allow  
the contents to equilibrate for 600 seconds.  
SOAK  
END  
End the wash protocol.  
Figure 9. Example of a Wash Protocol  
The procedures for specifying the steps in this and other wash protocols are  
described on the following pages.  
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Creating a New Wash Protocol  
To specify a Purge:  
Note: A purge must be performed outside of a  
Move cycle.  
1. Display the header information for the selected wash protocol, as  
MOVE  
2. Select PURGE. The display will read:  
Fluid volume? 350  
PURGE ꢀ  
3. Use the keypad to enter the volume of fluid to be dispensed.  
Note: A purge volume ranging from 50 to  
9,999 microliters can be entered.  
4. Press ENTER. The display will read:  
Select option  
MOVE  
PURGE ꢀ  
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Creating a New Wash Protocol  
To specify a Move cycle:  
1. Display the header information for the selected wash protocol, as  
MOVE  
2. Select MOVE. The display will read:  
Start strip?  
PURGE ꢀ  
1
3. Using the keypad, enter the strip number where the move cycle  
should start.  
4. Press ENTER. The display will read:  
End strip?  
12  
5. Using the keypad, enter the strip number where the move cycle  
should end.  
6. Press ENTER. The display will read:  
Cycles?  
1
7. Using the keypad, enter the number of the specified move cycles  
that are to be sequentially performed.  
Note: Between 1 and 9 Move cycles can be  
specified.  
8. Press ENTER. The display will read:  
Select option  
ASPIRATE DISPENSE  
9. Specify Aspirate, Dispense, Soak and Fill operations in the  
sequence as they are to be carried out, as described in the  
following sections.  
10. When all of the operations for the Move cycle have been  
sequentially specified, specify End Loop to designate the end of  
the Move cycle.  
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Creating a New Wash Protocol  
To specify an Aspiration (within a Move):  
Note: An Aspirate or Dispense must be  
performed within a Move cycle.  
1. Select ASPIRATE. The display will read:  
Cycles?  
1
2. Using the keypad, enter the number of aspiration cycles that are  
to be to be performed within the MOVE cycle.  
3. Press ENTER. The display will read:  
Select option  
ASPIRATE DISPENSE  
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Creating a New Wash Protocol  
To specify a Dispense (within a Move):  
Note: A Dispense or Aspirate must be  
performed within a Move cycle.  
1. Select DISPENSE. The display will read:  
Fluid volume? 350  
2. Use the keypad to enter the volume of fluid to be dispensed.  
Note: A volume ranging from 50 to 999  
microliters can be entered.  
3. Press ENTER. If a bottom wash is specified for the plate being  
used, the display will read:  
Bottom Wash?  
YES  
NO  
NO  
Note: If the protocol requires bottom washing,  
that must first be specified in the plate  
definition used in the protocol.  
If a bottom wash is not specified for the plate being used or if NO  
is selected for the Bottom Wash? prompt, the display will read:  
Select option  
ASPIRATE DISPENSE  
4. Select YES if a bottom wash is to be included. The display will  
read:  
Fluid volume?  
350  
5. Use the keypad to enter the volume of fluid to be dispensed  
during the bottom wash.  
Note: A volume ranging from 50 to 999  
microliters can be entered.  
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Creating a New Wash Protocol  
6. Press ENTER. The display will read:  
Cycles?  
1
7. Use the keypad to enter the number of bottom wash cycles to be  
performed and press ENTER. The display will read:  
Select option  
ASPIRATE DISPENSE  
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Creating a New Wash Protocol  
To specify a Soak (within a Move):  
Note: A Soak can be performed either within  
or outside of a Move cycle. The procedure  
below describes the procedure for specifying a  
soak within a Move cycle.  
The procedure for specifying a Soak outside  
of a Move cycle is described on page 50.  
1. Scroll right to display the next selection. The display will read:  
Select option  
SOAK  
2. Select SOAK. The display will read:  
Duration?  
FILL ꢀ  
5
3. Enter the desired soak duration using the keypad.  
Note: A soak duration ranging from 1 to 999  
seconds can be entered.  
4. Press ENTER The display will read:  
Select option  
SOAK  
FILL ꢀ  
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Creating a New Wash Protocol  
To specify a Fill (within a Move):  
Note: A Fill must be performed within a Move  
cycle.  
1. Scroll right to display the next selection. The display will read:  
Select option  
SOAK  
FILL ꢀ  
2. Select FILL. The display will read:  
Fluid volume?  
50  
3. Use the keypad to enter the volume of fluid to be dispensed  
during well filling.  
Note: A volume ranging from 50 to 300  
microliters can be entered.  
4. Press ENTER. The display will read:  
Select option  
SOAK  
FILL ꢀ  
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Creating a New Wash Protocol  
Ending a MOVE Cycle  
1. Scroll right to display the next selection. The display will read:  
Select option  
SOAK  
FILL ꢀ  
2. Scroll right to display the next selection. The display will read:  
Select option  
END LOOP  
3. Select END LOOP. The display will read:  
Select option  
MOVE  
PURGE ꢀ  
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Creating a New Wash Protocol  
To specify a Soak (outside of a Move):  
Note: A Soak can be performed either within  
or outside of a Move cycle. The procedure  
below describes the procedure for specifying a  
soak outside of a Move cycle.  
The procedure for specifying a Soak within a  
Move cycle is described on page 47.  
1. Scroll right to display the next selection. The display will read:  
Select option  
SOAK  
2. Select SOAK. The display will read:  
Duration?  
END  
5
3. Enter the desired soak duration (1 to 999 seconds) using the  
keypad.  
Note: A soak duration ranging from 1 to 999  
seconds can be entered.  
4. Press ENTER The display will read:  
Select option  
SOAK  
END  
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Creating a New Wash Protocol  
To specify the End of the wash protocol:  
1. Scroll right to display the next selection. The display will read:  
Select option  
SOAK  
END  
2. Select END. The display will read:  
1: NAME_01  
SETUP  
PROGRAM  
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Modifying a Wash Protocol  
Modifying a Wash Protocol  
An existing wash protocol can be modified by using many of the same  
commands that are used to create a wash protocol.  
To modify a wash protocol:  
1. Display the main menu:  
1: NAME_01  
SETUP  
PROGRAM  
2. Use the up and down scroll keys to select the wash protocol to  
be modified (or enter the number using the keypad). The display  
will read:  
4: NAME_04  
SETUP  
PROGRAM  
3. Select PROGRAM. If the wash protocol is password protected,  
the display will read:  
Enter password  
Note: If a Password is not specified for the  
selected wash protocol, the display will read  
as shown in Step 5.  
4. Enter the Password using the keypad and press ENTER.  
Note: If the Password is entered incorrectly an  
error message is displayed. The operator is  
allowed three attempts to enter the password.  
After the third attempt, the main menu  
(Step 1) will again be displayed.  
Note: If the password for a protocol is lost, the  
ability to access the method and the method  
position are also lost.  
5. After a password is successfully entered (or if a password was  
not specified for the selected protocol), the display will read:  
Edit name  
4: NAME_04  
COPY  
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Modifying a Wash Protocol  
6. The cursor is positioned under the first character of the name. If  
the name is to be changed, enter the name using the keypad and  
press ENTER. The first step of the wash protocol will be  
displayed:  
MOVE 1-12 1 Cycles  
DELETE  
EDIT ꢀ  
Note: The example above shows the MOVE  
operation as the first step in the protocol. The  
actual operation that is shown will depend  
upon the protocol that is being modified.  
Note: For each step that is displayed, the  
operation being carried out and the value for  
that operation are displayed. In the MOVE  
example above, the Start Strip is 1, the End  
Strip is 12, and the number of cycles is 1.  
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Modifying a Wash Protocol  
To modify a wash operation:  
1. Select EDIT while the operation is displayed. The first step in the  
operation is displayed:  
Start strip?  
1 Cycles  
Note: The actual step that is displayed  
depends upon the operation being modified.  
2. Modify the operation, using the same procedures as when  
creating a new wash protocol.  
MOVE 1-10  
DELETE  
1 Cycles  
EDIT  
Note: In the example above, the End Strip was  
changed from 12 to 10. The actual values that  
are displayed will depend upon the operation  
being modified and the new value(s) entered.  
Note: Once the move loop is modified, the  
display will no longer read as shown above.  
Instead, it will display the first command in the  
move loop or End of Move.  
3. Press ENTER to display the next step on the wash protocol.  
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Modifying a Wash Protocol  
To delete a wash operation:  
1. Select DELETE while the operation is displayed. The operation is  
removed from the wash protocol.  
Note: If a MOVE operation is deleted, all  
operations within the MOVE (i.e., Aspirate,  
Dispense, Soak and Fill) are also removed.  
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Modifying a Wash Protocol  
To insert a wash operation:  
1. Scroll right while the operation that the new operation is to  
precede is displayed:  
MOVE 1-10 1 Cycles  
INSERT  
Note: The inserted operation will take place  
immediately before the operation that is  
displayed.  
2. Select INSERT. The display will read:  
Select option  
MOVE  
PURGE ꢀ  
3. Select the option that is to be inserted, using the same procedure  
as when creating a new wash protocol.  
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Copying a Wash Protocol  
Copying a Wash Protocol  
An existing wash protocol can be copied to another program number. If a  
wash protocol is already in that location, it will be overwritten.  
To copy a wash protocol:  
1. Display the main menu:  
1: NAME_01  
SETUP  
PROGRAM  
2. Use the up and down scroll keys to select the wash protocol to  
be modified (or enter the protocol number using the keypad). The  
display will read:  
4: NAME_04  
SETUP  
PROGRAM  
3. Select PROGRAM. If the wash protocol is password protected,  
the display will read:  
Enter password  
4. Enter the Password using the keypad and press ENTER.  
Note: If the Password is entered incorrectly an  
error message is displayed. The operator is  
allowed three attempts to enter the password.  
After the third attempt, the main menu will be  
displayed.  
5. After a password is successfully entered, the display will read:  
Edit name  
4: NAME_04  
6. Select COPY. The display will read:  
Copy to?  
COPY  
7
Note: The protocol number that is displayed  
represents the first free protocol number, if any  
are available.  
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Copying a Wash Protocol  
7. Using the keypad, enter the number of the wash protocol that the  
commands are to be copied to. If a wash protocol already exists  
at that location the display will read:  
Overwrite test? NO  
YES  
NO  
8. Select YES. The display will read:  
7: NAME_04  
SETUP  
PROGRAM  
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Preparation  
Chapter 5 Washing a Plate  
Once the parameters for at least one plate type and one wash protocol have  
been set, the system can be used to wash a plate.  
Preparation  
The only preparation that is required is to check the fluid levels in the Wash  
Container and the Waste Container. Service the containers if necessary.  
To empty the waste container:  
1. Unscrew the cap of the Waste Container. Lay the cap and level  
sensor assembly on a paper towel.  
2. Discard the waste in accordance with local regulations.  
3. Rinse the waste container with deionized water. Discard the rinse  
water.  
Note: If desired, the waste container can be  
disinfected with a 10% (v/v) solution of  
household bleach in water.  
4. Place the level sensor and tube back into the Waste Container  
and screw the cap on.  
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Preparation  
To refill the wash container:  
1. Separate the dispense line quick-connector in the cap of the  
Wash Container.  
2. Unscrew the cap of the Wash Container and lay it and the Pick-  
Up Filter on a clean paper towel.  
3. Fill the Wash Container with the wash fluid that is being used.  
Note: If the wash solution is being changed,  
discard the contents of the Wash Container  
and thoroughly clean it before filling it with the  
new wash solution.  
4. Place the Pick-Up Filter into the Wash Container and screw the  
cap on. Re-connect the dispense line quick-connector.  
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Washing a Plate  
Washing a Plate  
A plate is washed by positioning it on the carrier block, selecting the wash  
protocol to be run, and pressing the START key.  
To wash a plate:  
1. (If needed) Turn on the instrument. The display will read:  
1: TESTNAME  
SETUP  
PROGRAM  
2. Place the microplate on the plate carrier.  
Note: Be sure that the plate position on the  
carrier plate corresponds to the wash head  
configuration that is being used. For example,  
if a 1 x 8 head is used, be sure that the plate is  
positioned with the columns aligned with the  
wash head rather than perpendicular to the  
wash head.  
3. Select the desired wash program using the up or down scroll  
keys or by entering the wash protocol number using the keypad.  
Note: Before starting the wash protocol, verify  
that the correct wash protocol name is  
displayed.  
4. Press the START key. The wash program will be automatically  
carried out.  
Note: If variable strips was specified for the  
wash protocol, you will be prompted to enter  
the strips that are to be washed before the  
wash will commence.  
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Washing a Plate  
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Routine Maintenance Procedures  
Chapter 6 Routine Maintenance  
Routine Maintenance Procedures  
The Opsys MW™ Microplate Washer is constructed from materials that  
resist chemical attack. If a spill occurs, it should be cleaned up as soon as  
possible using a cloth moistened with a mild laboratory detergent (If needed,  
dilute the laboratory detergent according to the manufacturer’s directions).  
The following periodic maintenance procedures are required for the  
Opsys MW:  
Daily maintenance:  
Verify that the self-test passes, by observing Arm movement and the  
absence of error messages.  
Clean the plate carrier, using a moist towel.  
Clean the external surfaces, using a moist towel.  
Weekly maintenance:  
Empty the Wash Container and clean it.  
The wash solution should be replaced at least once a week. The  
Wash Bottle should be cleaned whenever fresh wash solution is  
required.  
Note: If particulate matter is observed in the  
Wash Bottle, the wash solution should be  
discarded at once.  
To replace the wash solution:  
1. Discard any wash solution that is left in the Wash Bottle.  
2. Rinse the Wash Bottle with fresh solution.  
3. Fill the Wash Bottle with wash solution.  
4. Purge the system to ensure that all of the old solution is removed  
from the tubing.  
Empty the Waste Container and clean it.  
Inspect the tubing between the Wash Bottle and the Wash Head.  
Inspect the tubing between the Wash Head and the Waste  
Bottle.  
The tubing should be replaced if it has cracks, is cloudy,  
remains pinched, or is otherwise defective.  
Six month maintenance:  
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Routine Maintenance Procedures  
Replace the dispense (Wash) tubing.  
Replace the aspirate (Waste) tubing.  
Note: The dispense tubing and aspiration  
tubing may need to be replaced more  
frequently than every six months, depending  
upon the frequency of use and the severity of  
operating conditions.  
Contact DYNEX Technologies for information on  
replacement tubing.  
Replace the in-line hydrophobic filter.  
If the system will not be used for a period of time, the system should  
be flushed with de-ionized water so that buffers and/or reagents  
used to wash the plates are not deposited on the Wash Head or  
other components of the system.  
As required maintenance:  
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Cleaning and Decontamination  
Cleaning and Decontamination  
The Opsys MW™ is constructed from materials that resist chemical attack.  
Spills should be cleaned as soon as possible. If you need to decontaminate  
the Opsys MW™ instrument (for example, before servicing the instrument),  
clean the system and then decontaminate it as described below.  
CAUTION: Always disconnect the power cable  
before cleaning the instrument.  
To clean the system:  
1. Clean external surfaces with a cloth moistened with mild  
laboratory detergent.  
Note: If needed, dilute the laboratory  
detergent according to the manufacturer’s  
instructions before using.  
To decontaminate the system:  
1. Wipe the surfaces with a cloth moistened with 10% bleach or  
70% alcohol.  
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Cleaning the Containers  
Cleaning the Containers  
Procedures for emptying, cleaning and refilling the Wash Container and the  
Waste Container are described below.  
To empty and clean the waste container:  
1. Unscrew the cap of the Waste Container. Lay the cap and level  
sensor assembly on a paper towel.  
2. Discard the waste in accordance with local regulations.  
3. Rinse the waste container with deionized water. Discard the rinse  
water.  
Note: If desired, the waste container can be  
disinfected with a 10% (v/v) solution of  
household bleach in water.  
4. Place the level sensor and tube back into the Waste Container  
and screw the cap on.  
To refill the wash container:  
1. Separate the dispense line quick-connector in the cap of the  
Wash Container. Unscrew the cap and lay it and the Pick-Up  
Filter on a clean paper towel.  
2. Fill the Wash Container with the wash fluid that is being used.  
Note: If the wash solution is being changed,  
discard the contents of the Wash Container  
and thoroughly clean it before filling it with the  
new wash solution.  
3. Place the Pick-Up Filter into the Wash Container and screw the  
cap on. Reconnect the dispense line quick-connector.  
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Replacing the Tubing  
Replacing the Tubing  
To replace the dispense tubing:  
1. Separate the dispense line quick-connector in the cap of the  
Wash Container. Unscrew the cap, lay it and the Pick-Up Filter  
on a clean paper towel.  
2. Discard the contents of the Wash Container. Thoroughly rinse  
the container with deionized water and let it dry.  
3. Disconnect and discard the Wash Container Internal Tube and  
pick-up filter that is attached to the barb on the Quick Connect  
Fitting in the Cap.  
4. Replace the Wash Container Internal Tube and pick-up filter.  
5. Remove the Quick Connect Fitting from the Pump Tube that is  
attached to the Pump Inlet. Disconnect the tube from the pump  
and discard.  
6. Reattach the Quick Connect Fitting to a new length of Pump  
Tube and attach the free end of the tube to the Pump Inlet.  
7. Disconnect both ends of the Dispense Tube that is attached to  
the Pump Outlet and the Wash Head, remove the tubing from the  
dispense valve and discard.  
8. Attach the short length of larger diameter tube to the Pump  
Outlet and to the other end fit the larger barb of the Reducer  
Fitting. To the small end of the Reducer fit the long length of  
small diameter dispense tube and attach the free end to the  
Wash Head  
9. Route the dispense tubing through the upper slot in the front  
corner of the instrument and through the routing clips at the front  
of the instrument. Fit the dispense tubing in the dispense valve.  
10. Fill the Wash Container with fresh wash fluid. Place the Wash  
Container Internal Tube and pick-up filter back into the Wash  
Container and screw the Cap on. Reconnect the dispense line  
quick-connector.  
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Replacing the Tubing  
To replace the vacuum tubing:  
Note: This procedure describes replacement  
of the vacuum tubing connecting the Waste  
Container to the Foam/Aerosol Trap. The  
vacuum tubing connecting the Foam/Aerosol  
Trap to the rear of the instrument should not  
require changing.  
1. Unscrew the Cap of the Waste Container, lay it and the level  
sensor assembly on a clean paper towel.  
2. Discard the contents of the Waste Container in accordance with  
local regulations. Thoroughly rinse the container with deionized  
water and let it dry.  
3. Disconnect the vacuum tubing from the connector in the Cap and  
from the connector in the Foam/Aerosol Trap. Discard the old  
tube.  
4. Obtain a new length of vacuum tubing.  
5. Connect one end of the vacuum tubing to the connector in the  
Waste Container Cap and the other end to the connector in the  
Foam/Aerosol Trap.  
6. Place the level sensor assembly back into the Waste Container  
and screw the Cap on.  
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Replacing the Tubing  
To replace the waste tubing:  
1. Unscrew the Cap of the Waste Container and lay it with the level  
sensor assembly on a clean paper towel.  
2. Discard the contents of the Waste Container in accordance with  
local regulations. Thoroughly rinse the container with deionized  
water and let it dry.  
3. Disconnect the waste tubing from the connector on the Waste  
Container cap and from the waste tubing connector on the wash  
head.  
4. Remove the waste tubing from the instrument.  
5. Obtain a new length of waste tubing.  
6. Route the waste tubing through the lower slot at the front corner  
of the instrument and through the routing clips at the front of the  
instrument.  
7. Connect one end of the waste tubing to the waste tubing  
connector on the wash head and connect the other end to the  
connector in the Waste Container cap.  
8. Place the level sensor assembly back into the Waste Container  
and screw the Cap on.  
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Pump Calibration  
Pump Calibration  
Each Opsys MW™ washer manifold, or head, (e.g., 1 x 8, 1 x 12) has three  
calibration values stored in memory. These values are calculated and stored  
during manufacture at DYNEX Technologies. When the Opsys MW  
configuration is changed from one manifold to another, the corresponding  
calibration settings will be recalled by correctly setting the head size using  
the SETUP/HEAD feature (refer to page 28 for Specifying the Wash Head).  
This calibration protocol may be used at any time to verify the performance  
of the Opsys MW washer.  
IMPORTANT! It is recommended that this protocol be completed if any  
fluid-carrying component of the Opsys MW is replaced. This protocol may  
also be used to recalibrate the Opsys MW if a new wash buffer with different  
viscosity is used. Customize the protocol to emulate your assay, using the  
appropriate dispense volume, pump speed and fluid density.  
Calibration Protocol:  
1. Define a flat bottom plate profile using the SETUP/PLATE  
2. Program an assay to dispense 200µl/well into columns 1-12  
3. Obtain a clean, dry flat bottom 96-well styrene plate.  
4. Weigh the plate and record the empty plate weight in grams.  
5. Run the assay programmed in Step 2. (This assay will dispense  
200µl to all 96 wells in the plate.)  
6. Weigh the plate and record the filled plate weight in grams.  
7. Calculate the actual dispensed volume and % error using the  
following formulas:  
A.  
Actual Volume = [(Full weight – Empty weight) x 1000mg/g] /  
(96 wells x fluid density)  
B.  
%Error = 100 x (Actual Volume – Target Volume)/Target  
Volume  
Example:  
Empty plate weight: 48.38g  
Full plate weight: 67.42g  
Density of water at 20°C = 0.998mg/µl  
Actual Volume = [(67.42 – 48.38) x 1000]/(96 x 0.998) =  
198.7µl/well  
%Error = 100 x (198.7 – 200)/200 = -0.6%  
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Pump Calibration  
8. If the dispensed volume is too high, decrease the pump speed  
A pump speed change of -10 units roughly corresponds to a  
well volume change of -10µl.  
9. If the dispensed volume is too low, increase the pump speed  
A pump speed change of +10 units roughly corresponds to a  
well volume change of +10µl.  
10. Repeat Steps 3-9 until fluid is dispensed with the accuracy  
required. (e.g., Error less than or equal to ±5%)  
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Pump Calibration  
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Operational Problems  
Chapter 7 Troubleshooting  
Operational Problems  
Problems that may occur during normal operation of the Opsys MW™ are  
described below. For each problem, the symptom, probable cause and the  
means for resolving the problem are shown.  
Symptom  
Probable Cause Resolution  
The instrument  
fails to power up.  
The cord is not  
connected.  
Check that the cord is  
plugged into the  
instrument and into the  
electrical outlet.  
Verify that the electrical  
outlet is not controlled by  
a switch or timer.  
Verify that there is power  
at the electrical outlet by  
plugging in and checking  
another device.  
The powerstrip is Replace the powerstrip.  
faulty.  
The fuse is  
blown.  
Service is required.  
The breaker is  
tripped.  
Service is required.  
The power supply Reset the breaker.  
is defective.  
Liquid is not  
The dispense  
Remove the wash head  
dispensed from the pins are clogged. assembly and clean it  
dispense pins.  
using the manifold head  
cleaning wire. Then run a  
purge. Service is required  
if problem persists.  
The Wash  
Container is  
empty.  
Refill the Wash Container.  
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Operational Problems  
Symptom  
Probable Cause Resolution  
The wash Check the system  
Liquid is not  
dispensed from the protocol is  
configuration and wash  
protocol for the number  
fluids and fluid volume.  
dispense pins  
(continued).  
incorrectly  
defined.  
The dispense  
tubing or the  
dispense pump  
power supply is  
not connected.  
Verify that the dispense  
tubing and the dispense  
power supply are  
The dispense  
tubing is sealed  
shut at the  
Remove the tubing from  
the dispense valve and  
massage the tubing to  
release it. Replace the  
tubing into the dispense  
valve at a different  
dispense valve.  
location.  
Purge the tubing more  
frequently.  
Replace the tubing.  
Service is required.  
The dispense  
valve does not  
open.  
The wash head  
Remove the wash head  
manifold tubing is assembly and clean it  
blocked.  
using the manifold head  
cleaning wire. Then run a  
purge. Service is required  
if problem persists.  
There is a  
pressure leak.  
Check the seals and  
tubing for leaks.  
Check for air leaks.  
Reseat the tubing in the  
dispense valve.  
The dispense  
pump is faulty.  
Service is required.  
Liquid is dispensed The dispense  
Insert the dispense tubing  
into the dispense valve  
continuously from  
the dispense pins  
tubing is not  
inserted in the  
dispense valve.  
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Operational Problems  
Probable Cause Resolution  
Symptom  
Dispense volumes The dispense  
Check the pins, seals and  
tubing for leaks.  
are incorrect.  
pins are leaking.  
The dispense  
pressure is low.  
Check the seals and  
tubing for leaks.  
Check for air leaks.  
Reseat the tubing in the  
dispense valve.  
The dispense  
Remove the wash head  
pins are clogged. assembly and clean it  
using the manifold head  
cleaning wire. Then run a  
purge. Service is required  
if problem persists.  
Incorrect  
Recalibrate Pump  
Calibration  
The aspiration  
pins are clogged. assembly and clean it  
using the manifold head  
cleaning wire. Service is  
required if problem  
Aspiration is not  
complete.  
Remove the wash head  
persists.  
The aspiration pin Repeat the plate setup  
height is set too  
high.  
The seal on the  
Check the seal and waste  
waste container is tubing for leaks.  
leaking.  
The wash  
If the bottoms of the plate  
protocol is not  
optimized.  
wells are flat, use a  
sweep.  
Add additional aspiration  
cycles to the wash  
protocol.  
Aspiration tubing  
may be blocked  
or kinked.  
Check the aspiration  
tubing and remove any  
blockages or kinks.  
The vacuum  
exhaust may be  
blocked.  
Remove any obstructions  
The vacuum  
pump may be  
contaminated by  
overflow.  
Service is required.  
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Operational Problems  
Symptom  
Probable Cause Resolution  
Aspiration is not  
complete.  
Hydrophobic filter  
may be  
Replace filter.  
(continued)  
obstructed.  
The Waste  
Empty the Waste  
High Waste Level  
Container is full.  
is displayed.  
The waste level  
sensor cable is  
not plugged in.  
Connect the waste level  
sensor cable (see  
The waste level  
sensor connector  
is defective.  
Service is required.  
The waste level  
sensor detector is  
reversed.  
Service is required.  
The waste  
container is  
deformed.  
The waste tubing Remove the waste tubing  
is blocked.  
from the rear of the  
Check the waste tubing  
(and aspiration tubing)  
and remove any  
blockages or kinks.  
The waste  
container is  
damaged or  
defective.  
Replace the waste  
container.  
Motion of the  
wash head is  
being blocked.  
Check the wash head  
vicinity and remove any  
obstacles.  
X Motor Home  
Error is displayed.  
The motor is  
defective.  
Service is required.  
Motion of the  
wash head is  
being blocked.  
Check the wash head  
vicinity and remove any  
obstacles.  
Z Motor Home  
Error is displayed.  
The motor is  
defective.  
Service is required.  
The wash  
Reduce the number of  
Test Too Large is  
protocol exceeds steps in the wash protocol  
the memory limit. and try again.  
displayed.  
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Service Procedures  
Chapter 8 Service  
Service Procedures  
The only user service procedures that can be carried out for the Opsys  
MW™ are listed below:  
Removal and replacement of the wash head assembly  
Cleaning of the wash head assembly  
To remove the wash head assembly:  
1. Loosen the two thumbscrews at the front of the Arm/Head  
assembly.  
2. Grasp the wash head assembly and lift it up from the retaining  
pins on the Arm.  
3. Lift the wash head and tubing clear of the instrument. If  
necessary, remove the wash tubing and waste tubing from the  
retainer clips at the front of the instrument.  
To clean the wash head assembly:  
1. Pass the Cleaning Wire through the inside of each wash pin and  
waste pin on the wash head.  
2. Run a PURGE to rinse any material from the wash pins.  
To replace the wash head assembly:  
1. Position the wash head assembly back on the Arm pins.  
2. If necessary, replace the wash tubing and waste tubing into the  
retainer clips at the front of the instrument.  
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Spare Parts  
Spare Parts  
Accessories  
13001580  
13001610  
13001630  
352104000  
352101800  
91000051  
91000070  
23501800  
Plate Holder Assembly  
8-Way Head Assembly  
12-Way Head Assembly  
Cleaning Wire, 0.040”  
Cleaning Wire, 0.018”  
User Manual  
OPSYS MW Quick Set Up Instructions  
Bottle Wide Mouth (2120-0010, Nalgene, Wide Mouth  
Bottle w/Cap)  
029006501  
029027400  
200074300  
394000400  
32000170  
43000200  
43000390  
43000491  
43000540  
816400500  
816400600  
816400700  
62503160065  
43000540  
Jar, Aerosol Trap  
Bottle, Waste, 4L w/ Modified Top  
Rack, Bottle  
Sponge, Round, 60mm Diameter  
Stainless Steel Washer  
Fitting, Reducer, 3/16" X 3/32"  
Hydrophobic Filter  
Quick Connect Fitting (Dispense)  
Pick Up Filter  
Silicon, 5/64” ID x 1/8” OD (Dispense)  
Tubing, Silicon, 4 x 7.2 MM (Aspirate)  
Tubing, Silicon, 3 x 5 MM (Dispense)  
Quick Connect Fitting (Aspirate)  
Pick Up Filter  
Major Assemblies  
13001541  
13001555  
13001572  
43000320  
582050204  
582051001  
14000410  
14000420  
14000430  
Bottle Assembly  
Chassis Assembly  
X/Z Drive Assembly  
Dispense Pump  
PCB, Washer Main Board  
PCB, Washer Daughter Board  
Micro, Programmed, OPSYS MW Main PCB  
PLD, Programmed, OPSYS MW Main PCB  
PLD, Programmed, OPSYS MW Daughter PCB  
Hardware  
43000530  
528300700  
50500140  
564300100  
Pump, Vacuum, 12 VDC  
Stepper Motor  
LPS113 Power Supply, 12V/6.7A  
Float Switch, 0.5 AMP  
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Returning a Washer for Service  
Returning a Washer for Service  
If the instrument must be returned for service, it must be cleaned and  
decontaminated if it has been in contact with potentially infectious body  
fluids (including human blood), pathological samples, or toxic or radioactive  
materials.  
Note: Refer to page 69 for cleaning and  
decontamination instructions.  
To return a Washer for service:  
1. Contact the nearest DYNEX Technologies technical service facility  
for return authorization.  
2. Clean and decontaminate the Washer.  
3. Fill out an Equipment in Transit form (Figure 10)  
4. Pack the Washer and the Equipment in Transit form for  
shipment.  
5. After you receive a return authorization, ship the Washer to the  
nearest DYNEX Technologies facility (see page 85).  
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Returning a Washer for Service  
EQUIPMENT IN TRANSIT  
IMPORTANT: Please include a copy of this form with each instrument. If your  
instrument contains a hard drive, please retain back-up copies of any stored files.  
Failure to do so may result in the loss of those files  
.
Return Authorization Number:  
___________  
Contact Technical Service, DYNEX Technologies  
phone: (800) 288-2354 or (703)631-7800 ext. 1440  
fax: (703) 631-7816  
Equipment:  
__________  
Serial Number:  
_____________  
__________________________________________________________________  
EQUIPMENT DECLARATION  
Clearly indicate fault condition or reason for return  
.
________________________________________________________  
CERTIFICATE OF DECONTAMINATION  
I certify that the equipment described above has been disinfected/decontaminated*  
and is clean, dry and fit for transport.  
Signed:  
Title:  
Date:  
_
________________  
(DYNEX Technologies reserves the right to refuse improperly cleaned equipment)  
Shipping Address: DYNEX Technologies, Inc.  
Attn.: (Above return number)  
14340 Sullyfield Circle  
Chantilly, VA 20151-1683  
*Suggested decontamination methods:  
Readers- Wash all surfaces with a 10% Hypochlorite solution, Follow that with  
a mild detergent solution.  
Washers- Please follow the “Decontamination Procedure” found in the  
back of the manual.  
Figure 10. Equipment in Transit Form  
84  
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Contact DYNEX Technologies  
Contact DYNEX Technologies  
DYNEX Technologies  
14340 Sullyfield Circle  
Chantilly, VA 20151  
USA  
Tel: 703-631-7800  
Toll Free: 800-288-2354  
Fax: 703-803-1441  
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Index  
Index  
description, 1  
features, 7  
as required maintenance, 68  
ASPIRATE, 39  
aspiration height  
entering, 32  
illustration, 1  
overview, 1  
, 4  
programming, 2  
specifications, 9  
bottom wash  
specifying, 34  
bottom wash height  
, 4  
parity, 9  
CAL. command, 36  
password, 41, 56  
plate carrier, 15  
plate number  
calibration prototcol  
, 74  
cleaning  
, 69  
Wash Container, 70  
Waste Container, 70  
configuration, 28  
connections, 17  
displaying, 31  
plate parameters, 30  
specifying, 31  
positioning, 18  
power ON, 22  
Printer, 9  
daily maintenance, 67  
data bits, 9  
decontamination, 69  
DISPENSE, 39  
problems, 77  
, 41  
Program menu  
PROGRAM  
dispense height  
entering, 32  
, 4  
, 26, 27  
protocol number  
displaying, 42  
dispense pump, 20  
display panel, 15  
emptying  
Waste, 70  
End Loop, 47  
pump parameters, 36  
PURGE, 39, 45, 46  
replacing tubing, 71  
return of a washer, 83  
RS232, 9, 16  
error messages, 77  
FILL, 39  
scroll keys, 24  
fluid levels, 63  
foam/aerosol trap, 21  
footprint, 18  
function keys, 24  
hardware components, 15  
HEAD command, 29  
keypad, 16, 23  
Keypad, 9  
Setup menu, 26, 28  
Setup Menu, 27  
SHOW command, 30  
six month maintenance, 68  
SOAK, 39  
software version, 35  
spare parts, 82  
stop bits, 9  
main menu, 22  
maintenance, 67  
as required, 67  
daily, 67  
sweep  
entering, 32  
sweep height  
DYNEX Technologies Contacts,  
85  
timing, 41  
, 4  
six-month, 67  
weekly, 67  
MOVE, 39, 47  
name  
troubleshooting, 77  
tubing  
, 71  
usage area, 18  
vacuum tubing  
changing, 57  
Opsys MW  
, 72  
86  
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Index  
variable strips, 41  
warning labels, 11  
Wash Container, 5, 16  
cleaning, 64  
wash protocol  
copying, 61  
deleting, 59  
inserting, 60  
modifying, 56  
operations, 39, 45  
installing, 20  
refilling, 64  
wash fluid, 5  
wash head, 3, 15  
cleaning, 81  
installing, 19  
positions, 4  
wash protocols  
programming, 2  
washing a plate, 63, 65  
waste, 5  
Waste Container, 16  
removal, 81  
replacement, 81  
types, 3  
cleaning, 63  
emptying, 63  
waste tubing  
, 73  
weekly maintenance, 67  
well top  
wash pins, 3  
wash pins, 3  
, 4  
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