Sanyo Carbon Monoxide Alarm MCO 19AIC User Guide

INSTRUCTION MANUAL

CO₂Incubator

MCO-19AIC㩿UV㪀

MCO-19AIC

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INTRODUCTION

Ŷ Read this manual carefully before using the Product and follow the instructions for safety operation.

Ŷ Sanyo disavows any responsibility for safety if the Product is used for other than the intended use or

used with any procedures other than those given in this manual.

Ŷ Keep this manual in a suitable place so that it can be referred to as necessary.

Ŷ The contents of this manual are subject to change without notice for improvement of performance or

functions.

Ŷ Contact a Sanyo sales representative or agent if any page of the manual is lost or the page order is

incorrect.

Ŷ Contact a Sanyo sales representative or agent if any point in this manual is unclear or if there are any

inaccuracies.

Ŷ No part of this manual may be reproduced in any form without the expressed written permission of

Sanyo.

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PRECAUTIONS FOR SAFE OPERATION

It is imperative that the user complies with this manual as it contains

important safety advice.

Items and procedures are described so that you can use this unit correctly and safely.

If the precautions advised are followed, this will prevent possible injury to the user and

any other person.

Precautions are illustrated in the following way:

WARNING

Failure to observe WARNING signs could result in a hazard to personnel

possibly resulting in serious injury or death.

CAUTION

Failure to observe CAUTION signs could result in injury to personnel and

damage to the unit and associated property.

Symbol shows;

ꢀ

this symbol means caution.

this symbol means an action is prohibited.

this symbol means an instruction must be followed.

Be sure to keep this manual in a place accessible to users of this unit.

< Label on the unit >

This mark is labeled on the cover in which the electrical components of high voltage

are enclosed to prevent the electric shock.

The cover should be removed by a qualified engineer or a service personnel only.

WARNING

As with any equipment that uses CO₂gas, there is a likelihood of oxygen depletion in the vicinity

of the equipment. It is important that you assess the work site to ensure there is suitable and

sufficient ventilation. If restricted ventilation is suspected, then other methods of ensuring a

safe environment must be considered. These may include atmosphere monitoring and

warning devices.

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WARNING

Do not use the unit outdoors. Current leakage or electric shock may result if the unit is exposed to

rain water.

Only qualified engineers or service personnel should install the unit. The installation by

unqualified personnel may cause electric shock or fire.

Install the unit on a sturdy floor and take an adequate precaution to prevent the unit from

turning over. If the floor is not strong enough or the installation site is not adequate, this may result

in injury from the unit falling or tipping over.

Never install the unit in a humid place or a place where it is likely to be splashed by water.

Deterioration of the insulation may result which could cause current leakage or electric shock.

Never install the unit in a flammable or volatile location. This may cause explosion or fire.

Never install the unit where acid or corrosive gases are present as current leakage or electric

shock may result due to corrosion.

Always ground (earth) the unit to prevent electric shock. If the power supply outlet is not

grounded, it will be necessary to install a ground by qualified engineers.

Never ground the unit through a gas pipe, water main, telephone line or lightning rod. Such

grounding may cause electric shock in the case of an incomplete circuit.

Connect the unit to a power source as indicated on the rating label attached to the unit. Use

of any other voltage or frequency other than that on the rating label may cause fire or electric shock.

Never store volatile or flammable substances in this unit if the container cannot be sealed. These

may cause explosion or fire.

Do not insert metal objects such as a pin or a wire into any vent, gap or any outlet on the unit.

This may cause electric shock or injury by accidental contact with moving parts.

Use this unit in safe area when treating the poison, harmful or radiate articles. Improper use

may cause bad effect on your health or environment.

Turn off the power switch (if provided) and disconnect the power supply to the unit prior to any

repair or maintenance of the unit in order to prevent electric shock or injury.

Do not touch any electrical parts (such as power supply plug) or operate switches with a wet

hand. This may cause electric shock.

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WARNING

Ensure you do not inhale or consume medication or aerosols from around the unit at the time of

maintenance. These may be harmful to your health.

Never splash water directly onto the unit as this may cause electric shock or short circuit.

Never put containers with liquid on the unit as this may cause electric shock or short circuit when

the liquid is spilled.

Never bind, process, or step on the power supply cord, or never damage or break the power

supply plug. A broken supply cord or plug may cause fire or electric shock.

Do not use the supply cord if its plug is loose. Such supply cord may cause fire or electric shock.

Never disassemble, repair, or modify the unit yourself. Any such work carried out by an

unauthorized person may result in fire, or electric shock or injury due to a malfunction.

Disconnect the power supply plug if there is something wrong with the unit. Continued

abnormal operation may cause electric shock or fire.

When removing the plug from the power supply outlet, grip the power supply plug, not the cord.

Pulling the cord may result in electric shock or fire by short circuit.

Disconnect the power supply plug before moving the unit. Take care not to damage the power

cord. A damaged cord may cause electric shock or fire.

Disconnect the power plug when the unit is not used for long periods. Keeping the connection

may cause electric shock, current leakage, or fire due to the deterioration of insulation.

If the unit is to be stored unused in an unsupervised area for an extended period, ensure that

children do not have access and that doors cannot be closed completely.

The disposal of the unit should be accomplished by appropriate personnel. Remove doors to

prevent accidents such as suffocation.

Do not put the packing plastic bag within reach of children as suffocation may result.

Use the reagent specified by Sanyo for H₂O₂decontamination. Using a different H₂O₂solution

may result in explosion or damage to the Incubator.

When performing H₂O₂decontamination, securely close the internal and external doors. Failure to

do so may be harmful to health due to leakage of H₂O₂gas.

During H₂O₂decontamination, plug the access hole with the silicon cap that is provided. Failure

to do so may be harmful to health due to leakage of H₂O₂gas.

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CAUTION

Use a dedicated power source (a dedicated circuit with a breaker) as indicated on the rating label

attached to the unit. A branched circuit may cause fire resulting from abnormal heating.

Connect the power supply plug to the power source firmly after removing the dust on the plug.

A dusty plug or improper insertion may cause a heat or ignition.

Never store corrosive substances such as acid or alkali in this unit if the container cannot be

sealed. These may cause corrosion of inner components or electric parts.

Check the setting when starting up of operation after power failure or turning off of power

switch. The stored items may be damaged due to the change of setting.

Be careful not to tip over the unit during movement to prevent damage or injury.

Prepare a safety check sheet when you request any repair or maintenance for the safety of service

personnel.

Wear rubber gloves when handling the H₂O₂reagent. Direct contact with the H₂O₂reagent may

result in inflammation of the skin.

H₂O₂decontamination can be performed only for the chamber and chamber attachments with

standard specifications, and not for any other objects.

Perform H₂O₂decontamination with the chamber attachments arranged as specified by Sanyo.

Arranging them in a different way may result in insufficient decontamination.

After H₂O₂decontamination has been completed, wear rubber gloves and use a non-woven cloth

to wipe off the residual H₂O₂fluid from the bottom of the chamber, any objects that were

decontaminated, and the bottoms of ducts.

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LABELS ON THE INCUBATOR

Warning and caution labels are attached to the Incubator. The following table describes the labels.

This label is attached to covers that access high-voltage electrical components to

prevent electric shock. Only a qualified engineer or service personnel should be

allowed to open these covers.

This symbol indicates an ultraviolet light (UV) caution.

This symbol indicates that caution is required. Refer to product documentation for

details.

This symbol indicates a hot surface.

This symbol indicates an earth.

This symbol means “ON” for a power switch.

This symbol means “OFF” for a power switch.

EMVIRONMENTAL CONDITIONS

The Indicator is designed to be safe if used under the following conditions (based on the IEC 61010-1):

Ŷ Indoors

Ŷ Altitudes up to 2000 m

Ŷ Ambient temperature from 5 to 40^oC

Ŷ Maximum humidity of 80% for temperatures up to 31^oC decreasing linearly to maximum humidity of

50% at 40^oC

Ŷ Main power supply voltage fluctuations of r10% of the nominal voltage

Ŷ Other power supply voltage fluctuations as stated by the manufacturer

Ŷ Transient overvoltages according to Installation Category II

For the main power supply, the minimum and normal category is II.

Ŷ Pollution degree 2 according to IEC 60664.

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INCUBATOR COMPONENTS

9 (inside)

15, 16 (inside)

For MCO-19AIC(UV) or

when MCO-19UVS is installed.

13 (inside) 14

For MCO-19AIC(UV) or

when MCO-19UVS is installed.

Rear Right Side

Rear Left Side

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1. Outer door: The outer door is held to the frame with a magnetic seal. A door heater is installed in the

door panel. The door opening is reversible. Contact a Sanyo representative or agent to change the door

hinge from left to right or vice versa.

2. Inner door: The inner door is made of tempered glass. However do not subject the glass to excessive

impacts.

3. Leveling feet: The leveling feet can be turned to adjust the height. Adjust the feet so that the Incubator

is level.

4. Trays: The trays can be pulled forward.

5. Tray supports: The tray supports can be removed by lifting the front side and pulling toward you.

6. Side supports: The right and left side supports can be removed for disinfection. Refer to page 36 and

37.

7. Fan cover: The fan cover serves as the inlet for circulating air. It is removable.

8. Duct: The duct for the path for circulating air. It is removable.

9. Fan (inside the duct): The fan is made from polypropylene resin. It can be disinfected in an autoclave.

10. Sample air outlet: The sample air outlet also functions as an internal gas outlet. Normally, cover this

outlet with the sample air outlet cap.

11. Connecting port A/B for CO₂gas pipe: When the optional MCO-21GC Automatic CO₂Cylinder

Changeover System is installed, both ports A and B are available. If the MCO-21GC is not installed, only

port A is available. Refer to page 14 for gas cylinder connection. Ensure that the gas pressure is set at

0.03 MPaG (0.3 kgf/cm²G, 4.3 psiG). Refer to page 57 for automatic CO₂cylinder changeover system.

12. Door switch: The door switch detects when the door is open and stops the circulating fan and

electromagnetic valve for CO₂and UV lamp.*

13. Humidifying pan: Fill the humidifying pan with sterile distilled water. Install the pan properly so that it

can be covered with the pan cover.

14. Humidifying pan cover: The cover prevents UV light entering the chamber. When filling the pan, lift

the front side and take out the pan. Refer to page 39 for details.

15. UV lamp*: This Sanyo UV lamp does not generate ozone. Never look directly at the UV light. For

replacement, contact a Sanyo representative or agent.

16. Water level sensor for humidifying pan: This sensor detects the water level in the humidifying pan.

Refer to page 35 for details.

17. Remote alarm terminals: Refer to page 11.

18. Access port: Place the silicon caps on the port both outside and inside when the port is not being

used.

19. Power switch: This is the main switch for the Incubator. It also functions as an overcurrent breaker.

20. Glow starter*: The glow started if for the UV lamp (model FG-7P)

21. Attachment location for electric key: This is the attachment location for the electric key included in

the optional Component H₂O₂Decontamination Kit (MCO-HL). This kit must be attached to perform H₂O₂

decontamination. Refer to the installation procedure for MCO-HL for details.

22. Sample air outlet cap: Always attach this cap except when using the sample air outlet.

* MCO-19AIC(UV) or when an optional UV System Kit (MCO-19UVS) is installed.

100

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Control panel and keypad

1. LCD panel

7. CE Key:

Press this key to clear the entered value

when entering a setting.

2. Upper limit regulator:

This regulator is used to set the upper

temperature limit.

8. Cursor Keys (Up, Down, Left, and Right):

Use these keys to move the cursor on the

LCD panel.

3. OVERHEAT indicator:

This indicator lights when the chamber

temperature reaches the upper limit.

9. ENTER Key:

Press this key to select a menu command.

When entering a setting, pressing this key to

move to the next parameter.㩷

4. MENU Key:

Press this key to access the menu.

5. Contrast knob:

10. Numeric Keys

Turn this knob to adjust the contrast of the

LCD.

11. H₂O₂Key:

Press this key to start H₂O₂decontamination.

Refer to page 40 for details.

6. BUZZER Key:

Press this key to silence the buzzer.

Note: It is not possible to silence the

buzzer for an upper limit temperature

alarm.

㶎 The following optional components must be installed to perform decontamination.

䊶UV Lamp Add-on Kit (MCO-19UVS) (This is provided as standard equipment for the MCO-19AIC(UV).)

䊶H₂O₂Decontamination Kit (MCO-HL)

䊶H₂O₂Generator (MCO-HP)

101

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Remote alarm terminals

The remote alarm terminals are located at the rear right side.

Remote alarm

terminals

The remote alarm terminals are a contact output.

Normal:

Alarm:

Open

Closed

Contact capacity: 30 VDC, 2 A

Note:

• When the power switch is OFF or power is interrupted, the contact output will be closed.

• The remote alarm cannot be silenced by pressing the BUZZER Key since the remote alarm is not

conjunct with the BUZZER Key.

REMOTE ALARM

MAX DC30V 2A

Remote alarm terminals

N.O.

102

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INSTALLATION SITE

For correct operation of the Incubator, install it in a location with the following conditions.

WARNING

When using CO₂gas for control, make sure that there is adequate ventilation. Using CO₂gas in a

small room without adequate ventilation may cause gas poisoning or oxygen deprivation. In addition,

when opening the Incubator doors, do not directly inhale the air in the chamber.

Si l’appareil est utilisé dans un evdroit restreint, le niveau de la densite CO₂de l’air peut s’élever et peut

être nocif aux humains. Evitez d’aspirer l’air provenant de l’inérieur de l’appareil quand vous ouverz la

porte.

z Normal air environment

Install the Incubator in an environment with normal air.

z Do not expose to direct sunlight

Do not install the Incubator in a location where it will be exposed to direct sunlight. If the Incubator is

operated in direct sunlight, performance will be adversely affected.

z Separate from heat sources

Do not install the Incubator near significant heat sources, such as heaters, boilers, ovens, or autoclaves.

Heat will adversely affect the performance of the Incubator.

z Ambient temperature at least 5qC lower than set temperature

The control temperature of the Incubator is at least 5qC higher than the ambient temperature. For

example, if the chamber is controlled at 37qC, the ambient temperature must normally be no more than

32qC. Do not allow the ambient temperature to become too high.

z Strong and level floor

Select a site with a strong and level floor. If the floor is uneven or the installation is not level, the Incubator

will be unstable and this may cause accident or injury. To avoid vibration and noise, always make sure

that the installation is stable. An unstable surface may result in vibration or noise.

WARNING

Install the Incubator at a location that can support the weight. If the floor is not strong enough or if the

installation is insufficient, the Incubator may fall over and cause injury.

Always make sure that the floor is strong, even, and level, and that the Incubator will not tip over.

An insufficient installation may result in injury due to water leakage or the Incubator falling over.

z Low humidity

Select a site with a relative humidity of 80% or lower. Using the Incubator in high humidity may result in

current leakage or electric shock.

WARNING

Do not use the Incubator outdoors. If the Incubator is exposed to rain water, it may result in current

leakage or electric shock.

Never install the Incubator in a moist location, such as near a sink or water line, or where it is

likely to be exposed to water. In addition, do not install it near water or steam pipes. Moisture can

_causethe insulation to deteriorate, which may result in current leakage or electric shock.

z No inflammable or corrosive gas

Never install the Incubator in a location where it will be exposed to inflammable or corrosive gas. Doing

so may result in explosion or fire. In addition, insulation may deteriorate due to corrosion of protective

casing, resulting in current leakage or electric shock.

z No falling objects

Do not install the Incubator in a location where there is the possibility of objects falling from above. Doing

so may result in damage or accident.

103

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INSTALLATION

1. Remove the packing tape and clean up.

Remove all the tape that is securing the doors and inner attachments. Open the doors for ventilation. If

the outer panels are dirty, dampen a cloth with a diluted neutral detergent and wipe them. (Using an

undiluted solution may damage the plastic. Follow the diluting instructions for the detergent that is used.)

Wipe off the residual detergent with a wet cloth and then wipe off any moisture.

2. Adjust the leveling feet.

Adjust the leveling feet by turning them counterclockwise to

level the

Incubator. (See the illustration on the right.)

3. Ground the Incubator.

Ground the Incubator during installation to prevent electric

shock in case the insulation is not sufficient. If there is no

ground wire at the location, consult with qualified service

personnel.

Leveling feet

z When a ground must be installed

If a grounded 3-pole outlet is not available, then a ground must be installed. Consult with qualified service

personnel.

WARNING

To prevent electric shock, always ground the Incubator. If grounding is not possible, then have a ground

installed by qualified personnel. If the Incubator is not grounded, it may result in electric shock.

Never connect the ground wire to a gas pipe, water pipe, lightning rod, or telephone ground wire.

Doing so may cause electric shock_.

z Installing a ground fault circuit breaker

If using the Incubator in a location with moisture or humidity cannot be avoided, then it is recommended

that a ground fault circuit breaker be installed in the power supply circuit (i.e., the power supply at the

Incubator). Have the circuit breaker installed by qualified service personnel.

CAUTION

Do not climb on the Incubator or place objects on top of it. Doing so may damage it or cause it to fall

over, resulting in injury. If it is to be stacked, refer to page 62 and stack it securely.

z When the Incubator is not in use

Empty the water from the humidifying pan and remove moisture from the chamber. Make sure that the

chamber is completely dry before closing the doors. Failure to do so may result in damage.

z Before moving the Incubator

Before moving the Incubator, empty the water from the humidifying pan, disconnect the power supply plug

from the outlet, and make sure that the cord will not be damaged. Failure to do so may result in electric

shock or fire.

104

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Connecting a CO₂gas cylinder

WARNING

When connecting a gas cylinder to the Incubator, confirm the gas type. Confirm that the connections

are secure and that no gas will escape. Be sure to use the specified pressure. Using an incorrect

gas or pressure may result in explosion or fire, or in gas poisoning or oxygen deprivation due to escaping

gas.

Install the Incubator in a location with adequate ventilation. If adequate ventilation cannot be

provided, then install an alarm system using CO₂and O₂densitometers.

1. Use a liquefied CO₂gas cylinder (at least 99.5% pure). The siphon (dip tube) type cannot be used.

2. Install the Gas Pressure Regulator (MCO-100L, purchased separately) specified by Sanyo on the CO₂

gas cylinder. Otherwise, use a CO₂gas pressure regulator rated at 25 MPaG (250 kgf/cm²G, 3600 psiG)

for the primary pressure, and 0.2 MPaG (2 kgf/cm²G, 30 psiG) for the secondary pressure.

3. Using the gas supply pipe that is provided, connect the CO₂gas pressure regulator to the CO₂gas pipe

inlet (located at the lower left-hand side on the rear panel of the Incubator).

Note:

If CO₂is supplied to multiple CO₂Incubators from a single gas cylinder, a CO₂solid will be formed in the

gas pressure regulator. The gas pressure regulator safety valve will operate, and there may an explosive

sound.

When the MCO-21GC is not mounted

Using the gas supply pipe that is provided, connect the CO₂gas pressure regulator for the CO₂gas

cylinder to CO₂gas pipe connection port A on the Incubator. After connecting the pipe, check to make

sure that no gas is leaking.

When the MCO-21GC is mounted

Connect a pair of CO₂gas cylinders with CO₂gas pressure regulators independently. The gas supply line

will be switched automatically. Connect the main cylinder to port A and the reserve cylinder to port B. After

connecting the cylinders, check to make sure that no gas is leaking.

z For details on installing the optional automatic CO₂Cylinder Changeover Kit (MCO-21GC), refer to the

MCO-21GC installation guide. For details on using the MCO-21GC, refer to page 57.

4. Set the CO₂gas on the secondary side to 0.03 MPaG (0.3 kgf/cm²G, 4.3 psiG) for gas injection. As the

pressure increases, the CO₂gas concentration control range will increase. Excessive pressure may

cause gas supply lines inside the Incubator to come loose, which may result in gas poisoning or oxygen

deprivation due to the escaping of gas. If gas lines come loose, the Incubator must be repaired.

z The gas lines connected to the Incubator will degrade over time. If any deterioration or abnormalities

are found during inspection, replace the lines immediately.

105

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PREVENTING CONTAMINATION

To prevent contamination of the chamber, select a suitable installation site.

z Avoid locations with high temperatures or humidity.

Avoid locations with high temperatures or humidity, because of a greater presence of microorganisms in

the air.

z Avoid locations with passers-by or drafts.

Avoid locations near doors, air conditioners, fans, etc., where passers-by or drafts can facilitate the entry

of microorganisms into the chamber.

z If possible, use a cleanroom.

To achieve a better culture, it is recommended that a cleanroom be used if one is available.

z Use clean containers.

The greatest cause of contamination is dirty containers for cultures. Be careful not to get containers or

trays dirty when taking them in and out.

z Keep the chamber clean.

Wipe off any fingerprints. If water spills from the humidifying pan, or if the doors are left open for a long

time, condensation may form on the inside of the doors. If that occurs, wipe off the condensation with a

dry sterile gauze. In particular, clean and disinfect the chamber if the culture medium is spilled. For details,

refer to Routine Maintenance on page 36.

z Use sterile distilled water in the humidifying pan.

Always use sterile distilled water in the humidifying pan. Do not use ultrapure water, because it may

contain red rust-like suspended particles.

A water level alarm is displayed in the status display area on the control panel. Quickly refill the water in

the pan when the water level alarm is displayed. Adding low-temperature water will significantly lower the

temperature in the chamber. Clean the humidifying pan once a month.

z Keep the Incubator out of direct airflows from air conditioners or fans.

Cool airflow from an air conditioner may cause condensation and lead to possible contamination.

106

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PRECAUTIONS FOR CULTURES

z Leave space between culture containers.

Always leave space for ventilation between culture containers (Petri dishes, flasks, etc.). Inadequate

spacing may result in uneven temperature distribution and CO₂gas concentration.

z Do not place harmful materials in the chamber.

Never place samples that release acidic, alkali, or corrosive gas in the chamber. Doing so may cause

damage resulting from discoloration or corrosion.

z Close the inner door.

Always close the inner door before closing the outer door. Failure to close the inner door will adversely

affect performance even if the outer door is closed.

z Open and close the doors gently.

Always open and close the doors gently. Closing the doors forcefully may cause spillage of the culture

medium, incomplete closing, or damage to the gasket. Before opening the inner door, check through the

glass to confirm that the UV lamp is OFF (if the MCO-19AIC(UV) or the optional MCO-19UVS is

installed).

z Be careful when closing the outer door.

Use the handle when closing the outer door. Holding the door in other places may cause injury by getting

fingers caught in the door. Do not lean on the outer door. Doing so may result in injury from the outer door

coming loose or the Incubator falling over, or it may cause current leakage or electric shock.

z Be careful of the inside of the outer door.

The inside of the outer door may become hot.

z Avoid using excessive force on the inner door.

Do not put your hand on the glass, poke it with sharp objects, or apply strong force. Doing so may result

in injury from breaking the glass.

z Check the cause of any alarm buzzer.

If an alarm buzzer sounds while the Incubator is in use, immediately check the cause of the alarm. For

details on what may cause an alarm buzzer to sound, refer to page 46.

107

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Using the Unlock Key

z Unlocking when power is interrupted

If power is interrupted to the MCO-19AIC(UV)/19AIC with an MCO-HL installed, the outer door is

electrically locked. To unlock the outer door while the power is interrupted, use the unlock key that is

provided. To lock the outer door again, turn the unlock key to the lock direction while the door is open.

After the door has been locked condition manually, then close the door.

Note:

The outer door cannot be locked by using the unlock key while the door is closed. Lock the door while it is

open. Attempting to turn the unlock key while the door is closed may damage the electric lock system.

WARNING

For the MCO-19AIC(UV)/19AIC with an MCO-HL installed, the outer door is electrically locked during

decontamination. The chamber is filled with H₂O₂gas during decontamination. Do not unlock the door

during decontamination.

㩷

CORRECT OPERATION

Use the following procedure to start trial operation or actual operation of the Incubator.

1. Install the Incubator correctly, referring to Installation on page 13.

2. Remove the packing materials from the chamber and inner attachments. Clean and disinfect the

chamber and inner attachments, referring to Routine Maintenance on page 36.

3. Add approximately 1.5 L of sterile distilled water to the humidifying pan. (Refer to page 39.)

4. Turn ON the power supply switch on the left side of the rear panel of the Incubator.

Note:

The humidity in the Incubator chamber is adjusted to the optimum setting. To prevent condensation on

the surface inside chamber and the inner door, there is a low-temperature area under the humidifying pan

in the bottom of chamber to recondense evaporated moisture. Condensation may occur around the

humidifying pan at the bottom of the chamber (on the inside of the humidifying pan cover), but this does

not indicate a problem.

WARNING

Do not leave plastic wrapping bags within reach of children. If a bag is placed over a child’s head, it

can block the mouth and nose and cause suffocation.

108

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LCD PANEL㩷

㩷

The following display (called the Top Display) will appear when the power switch is turned ON. The

default temperature is 37.0^oC and the default CO₂density is 0%. The date and time are preset at the

factory. Refer to page 30 to change the date and time.㩷㩷

㩷

T e m p

3 7 .

C O 2

5 . 0 %

ꢁꢁ㧡ꢀ㧜ꢁ

A N

㧟㧣ꢀ㧜ꢁ

S t a t u s

U V

R H

D o o r : O p e n

O K

1. Display of set value of temperature

The set value of chamber temperature is displayed.

2. Display of current temperature

The current chamber temperature is displayed.

3. Display of set value of CO₂density

The set value of the chamber CO₂density is displayed.

4. Display of current CO₂density

The current chamber CO₂density is displayed.

5. Display of current CO₂cylinder

A and B will be displayed if the optional Automatic CO₂Cylinder Changeover System (MCO-21GC) is

installed. The port of the CO₂gas pipe that is currently supplying CO₂will be displayed in reverse video.

Nothing will be displayed if the MCO-21GC is not installed.

6. Status display field

Various status or alarms are displayed.

䊶When UV lamp is lit: “UV” is displayed in reverse video.

䊶When humidifying water is low: “RH PAN” is displayed in reverse video.

䊶When the door is open: Door: Open” is displayed in reverse video.

“

7. Message display field

A message is displayed when fault occurs. The message is displayed alternately in normal characters

and reverse video. Refer to pages 46 and 47 for alarm details. OK is displayed during normal

“

”

operation. 㩷

㩷

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BASIC OPERATIONS ON CONTROL PANEL㩷

㩷

The following operation are possible through control panel:㩷

㩷

1. Setting the temperature䊶䊶䊶The chamber temperature can be set (page 20).

2. Setting the CO₂density䊶䊶䊶The chamber CO₂density can be set (page 20).

3. Setting the key lock䊶䊶䊶Changing the temperature and CO₂density can be disabled (page 21).

4. Setting alarms䊶䊶䊶The temperature alarm, CO₂density alarm (page 25), and upper limit alarm

temperature (page 24) can be set.

5. Setting UV parameters䊶䊶䊶ON/OFF parameters and the ON period of the UV lamp can be set for the

MCO-19AIC(UV) or when the Incubator is equipped with the optional MCO-19UVS (page 26).

6. Setting other parameters䊶䊶䊶The initial settings of the date, time, and log cycle (page 30), and the

LCD display and baud rate (page 32) can be set.

7. Displaying the operation log and transferring data䊶䊶䊶A graph of past operation data can be

displayed (page 33) and operation data can be transferred to a PC (page 34).

8. H₂O₂decontamination䊶䊶䊶H₂O₂decontamination of the chamber and internal attachments can be

performed when the optional H₂O₂Generator (MCO-HP) is installed (page 40).

9. Automatic CO₂cylinder changeover䊶䊶䊶The cylinder can be switched when the optional automatic

CO₂Cylinder Changeover System (MCO-21GC) is installed (page 57).

10. Setting the CO₂standard gas density䊶䊶䊶The CO₂standard gas density can be set when the

optional Automatic CO₂Standard Gas Calibration Kit (MCO-SG) is installed. (page 59)

㩷

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BASIC PARAMETERS

㩷

㩷 Setting the chamber temperature and CO₂density㩷

㩷

The setting procedure for the chamber temperature and CO₂density are given below. (Default settings:㩷

Chamber temperature: 37^oC, CO₂density: 0%)㩷

The Incubator automatically starts operation using these settings after the power is turned ON.

㩷

1. From the Top Display, press the MENU Key to access the menu. Select Set and press the ENTER

Key.

㩷

T e m p

3 7 .

C O 2

5 . 0 %

㩷

M E N U

S e t

ꢁꢁ㧡ꢀ㧜ꢁ

㧟㧣ꢀ㧜ꢁ

L o g

T o o l

S t a t u s

O K

㩷

2. The Stand-By Setting Display will be displayed. Set the parameters.㩷

㩷

S t a n d - b y

S e t

n g

M E N U

C )

㩷

T e m p e r a t u r e

3 7 .

(

0 .

C - 5 0 .

O K

C O 2

D e n s i

L o c k

L i m i

5 . 0 %

0 . 0 %

2 0 . 0 % )

C a n c e l

K e y

(

0 . U n l o c k

1 . L o c k )

H i g h

5 2 .

㩷

The setting ranges of the parameters are as follows:

ƔTemperature: 0 to 50^oC. To 37.0^oC, enter 370.

ƔCO₂density: 0 to 20%. To set 5.0%, enter 050.㩷

㩷

3. Press the MENU Key to display the menu after setting the parameters. Select OK and press the

ENTER Key. The settings will be saved.

㩷

Note:

When starting the Incubator for the first time or after not using it for an extended period of time, allow at

least 4 hours for the chamber temperature, humidity, and CO₂sensor to become stable after setting the

desired chamber temperature at a 0% CO₂density. Then change the setting to the desired CO₂density.

㩷

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㩷

㩷㩷 Setting the key lock㩷

㩷

1. To set the key lock, change the value of the key lock parameter from 0 to 1 on the Stand-by Setting

Display and press the ENTER Key. The buzzer will sound briefly and the keys will be locked.

㩷

S t a n d - b y

S e t

n g

㩷

M E N U

C )

T e m p e r a t u r e

3 7 .

(

0 .

C - 5 0 .

O K

C O 2

D e n s i

L o c k

L i m i

5 . 0 %

0 . 0 %

2 0 . 0 % )

C a n c e l

K e y

(

0 . U n l o c k

1 . L o c k )

H i g h

5 2 .

2. “Key Lock” will be displayed at the right side of “Stand-by Setting”, and changing the temperature and

CO₂density settings will be disabled.

㩷

㩷㩷 Removing the key lock㩷

㩷

1. To remove the key lock, change the value of the key lock parameter from 1 to 0 on the Stand-by

Setting Display and press the ENTER Key.

㩷

S t a n d - b y

S e t

n g

K e y

L o c k

㩷

T e m p e r a t u r e

3 7 .

(

0 .

C - 5 0 .

C )

C O 2

D e n s i

L o c k

L i m i

5 . 0 %

0 . 0 %

2 0 . 0 % )

K e y

(

0 . U n l o c k

1 . L o c k )

H i g h

5 2 .

2. The cursor will move to the Password Field. Input the 4-digit password (default: 0000) and press the

ENTER Key. When key lock is release, the buzzer will sound briefly, and “Key Lock” will disappear from

the Stand-by Setting Display.

㩷

S t a n d - b y

S e t

n g

K e y

L o c k

T e m p e r a t u r e

3 7 .

(

0 - 5 0 .

0 . 0 %

C )

C O 2

D e n s i

L o c k

L i m i

5 . 0 %

2 0 . 0 % )

K e y

P a s s w o r d

H i g h

5 2 .

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㩷

Note: The buzzer will sound for a long time if the wrong password is entered. Enter the correct password.

The password for releasing the key lock must be shared and administered among all users of the

Incubator. The password is set to 0000 when the Incubator is shipped from the factory. The procedure for

changing the password is given next.

㩷㩷 Setting the key lock password

㩷

1. From the Top Display, press the MENU Key to display the menu, select Tools, and press the ENTER

Key.

㩷

T e m p

3 7 .

C O 2

5 . 0 %

㩷

M E N U

S e t

ꢁꢁ㧡ꢀ㧜ꢁ

㧟㧣ꢀ㧜ꢁ

L o g

T o o l

S t a t u s

O K

2. Select Key Lock PW Setting from the Select Tools Display, press the MENU Key to display the menu,

select OK, and press the ENTER Key.

㩷

S e l e c t

T o o l

C a l

n g

(

1 / 2 )

㩷

T e m p / C O 2

b r a t o n

M E N U

O K

A l a r m

S e t

L C D / D A Q

S e t

n g

S v C

U V

S e t

L o c k

T i m e

C a n c e l

K e y

D a t

P W

S e t

n g

3. Input the current user password (4 digits), select OK, and press the ENTER Key. (The default user

password is 0000 when the Incubator is shipped from the factory.)

㩷

K e

L o c k

P W

S e t

n g

㩷

C u r r e n t

U s e r

P a s s w o r d

㧖㧖㧖㧖

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㩷

4. Enter the new user password (4 digits), select OK, and press the ENTER Key.

㩷

K e

L o c k

U s e r

P W

S e t

n g

㩷

N e w

P a s s w o r d

㧖㧖㧖㧖

5. Enter the new user password again, select OK, and press the ENTER Key.

㩷

K e

L o c k

P W

S e t

n g

㩷

N e w

R e

U s e r

P a s s w o r d

㧖㧖㧖㧖

E n t e r

U s e r

P a s s w o r d

㩷

Note:

Be careful not to forget the key lock password. If you have forgotten the password and cannot release the

key lock, contact a Sanyo sales representative or agent.㩷

㩷

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㩷

Setting the upper limit alarm temperature

㩷

An upper limit temperature alarm is provided with the Incubator. The alarm temperature can be changed

by using the following procedure.

㩷

1. In the Stand-by Setting Display, turn the upper limit regulator on the control panel using a small

screwdriver to set the desired upper limit alarm temperature. The upper limit temperature can be set

between 34 and 52^oC.

㩷

S t a n d - b y

S e t

n g

㩷

M E N U

C )

T e m p e r a t u r e

3 7 .

(

0 .

C - 5 0 .

O K

C O 2

D e n s i

L o c k

L i m i

5 . 0 %

0 . 0 %

2 0 . 0 % )

C a n c e l

K e y

(

0 . U n l o c k

1 . L o c k )

H i g h

5 2 .

㩷

Note:

䊶Set the upper limit alarm temperature (High Limit) to at least 5^oC higher than the chamber set

temperature.

䊶Set the upper limit alarm temperature to at least 50^oC when H₂O₂decontamination is performed.

2. After setting the parameter, press the MENU Key to display the menu, select OK, and press the

ENTER Key. The alarm temperature will be saved.

Refer to the tables of alarms and safety functions on page 46 for details.

Note:

䊶The alarm temperature will be changed at any time by turning the upper limit regulator even if the

Stand-by Setting Display is not displayed.

䊶Set the upper limit alarm temperature after the chamber reaches the set temperature for operation.

㩷

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ALARM PARAMETERS㩷

㩷

1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTER

Key.

㩷

T e m p

3 7 .

C O 2

5 . 0 %

㩷

M E N U

S e t

ꢁꢁ㧡ꢀ㧜ꢁ

㧟㧣ꢀ㧜ꢁ

L o g

T o o l

S t a t u s

O K

2. Select Alarm Setting from the Select Tools Display, press the MENU Key to display the menu, select

OK, and press the ENTER Key.

㩷

S e l e c t

T o o l

C a l

n g

(

1 / 2 )

㩷

M E N U

O K

T e m p / C O 2

b r a t o n

A l a r m

S e t

L C D / D A Q

S e t

n g

S v c

U V

S e t

L o c k

T i m e

C a n c e l

K e y

D a t

P W

S e t

n g

3. The Alarm Setting Display will appear. The temperature alarm, CO₂alarm, alarm delay, ring back time,

and door delay can be set on this display. The alarm buzzer can be silenced by pressing the BUZZER

Key. The buzzer will sound again after the specified ring back time if the condition that caused the alarm

continues. The ring back time can be set.

㩷

A l a r m

T e m p

S e t

n g

M E N U

A l a r m

1 .

0 .

(

1 . 0 ° C

0 . 5 %

㧙

5 . 0 ° C 㧕

O K

C O 2

A l a r m

㧙

5 . 0 % 㧕

C a n c e l

A l a r m

D e l a y

B a c k

D e l a y

1 5 m i

3 0 m i n

2 m i

(

1 5 m i n )

0 . O F F 1 㧙 9 9 m i n )

3 0 m i n )

R i

D o o r

䊶Temperature alarm: 1.0 to 5.0^oC (Default: 1.0^oC)

䊶CO₂alarm: 0.5 to 5.0% (Default: 1.0%)

䊶Alarm delay: 0 to 15 minutes (Default: 15 minutes)

䊶Ring back time: 1 to 99 minutes, or OFF (Default: 30 minutes)

䊶Door alarm delay: 1 to 30 minutes (Default: 2 minutes)

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UV LAMP PARAMETERS

㩷

A UV lamp is used with the MCO-19AIC(UV) or the MCO-19AIC with an MCO-19UVS UV Lamp

Expansion Kit installed. Use the following procedures to make the settings.

㩷

㩷㩷 Using the UV lamp㩷

㩷

A UV lamp is located inside the duct to sterilize the water in the humidifying pan and the air circulating in

the chamber. Observe the following points to use the UV lamp correctly.

z When all chamber attachments are installed correctly, only the inside of the duct and the inside of the

humidifying pan cover are exposed to UV light.

z Correctly install all of the chamber attachments when starting a cell culture, and never turn ON the UV

lamp when the humidifying pan cover is removed.

z Always install the humidifying pan cover even when using the Incubator without turning ON the UV

lamp. Leaving the cover uninstalled will affect the chamber temperature distribution and the humidity

recovery performance.

z The UV lamp stays lit for a preset period of time after the outer door is closed. The default setting is for

five minutes.

z If the outer door is not opened for at least 12 consecutive hours, the UV lamp will light for the preset

time period every 12 hours.

z The recommended replacement time for the UV lamp (i.e., when the UV output ratio drops to 60% to

70% of its initial value) is when the accumulated ON time reaches 1,000 hours. When the accumulated

ON time reaches approximately 1,000 hours, the “UV” will flash in the status display field on the LCD

panel (when the UV lamp is not lit). It is recommended that the UV lamp be quickly replaced at this point.

Consult a Sanyo sales representative or agent for information on replacing the UV lamp.

z If the UV lamp burns out, Err18:UV Lamp Abnormal will be displayed in the message display field. If

this occurs, replace the UV lamp. When replacing the UV lamp, replace the glow starter (type FG-7P) at

the same time. Consult a Sanyo sales representative or agent for information on replacing the UV lamp.

z If the UV lamp burns out (Err18:UV Lamp Abnormal will be displayed in the message display field), it

will not be possible to perform H₂O₂decontamination. Replace the UV lamp and the glow starter.

㩷

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㩷

㩷㩷 Precautions when using the UV lamp

㩷

WARNING

Do not look directly at UV light. UV light is harmful to the eyes.

z Always use the humidifying pan and humidifying pan cover.

The humidifying pan and cover prevent UV light from escaping. Always use them even when not

humidifying. To check whether the UV lamp is lit, open the outer door and then press the door switch with

the inner door still closed. Visible blue light can be confirmed from the front of the humidifying pan cover.

UV light is harmful to the eyes, so do not light the UV lamp when the inner door or humidifying pan cover

is open.

z Be careful when handling the UV lamp.

There is a UV lamp inside of the chamber duct. Be careful not to damage it when installing or removing

chamber attachments, the humidifying pan, or the H₂O₂Generator.

㩷

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㩷

Setting the UV lamp ON period

㩷

Use the following procedure to change the setting of the UV lamp ON period.㩷

㩷

1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTER

Key.

㩷

T e m p

3 7 .

C O 2

5 . 0 %

㩷

M E N U

S e t

ꢁꢁ㧡ꢀ㧜ꢁ

㧟㧣ꢀ㧜ꢁ

L o g

T o o l

S t a t u s

O K

2. Select UV Setting from the Select Tools Display, press the MENU Key to display the menu, select OK,

and press the ENTER Key.

㩷

S e l e c t

T o o l

C a l

n g

(

1 / 2 )

㩷

T e m p / C O 2

b r a t o n

㩷

M E N U

O K

A l a r m

S e t

L C D / D A Q

S e t

n g

S v c

U V

S e t

L o c k

T i m e

C a n c e l

K e y

D a t

P W

S e t

n g

3. The UV Setting Display will appear. Set the UV Timer for the UV lamp ON period.

㩷

U V

S e t

T i m e r

L i

T i m e r

n g

㩷

M E N U

3 0 m i n )

U V

m i

(

O K

C a n c e l

E x t

U V

2 4 h

M o d e

(

0 . O F F

1 . O N )

4. Press the MENU Key to display the menu, select OK, and press the ENTER Key. The parameter will

be saved.

㩷

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㩷

x The UV timer can be set from 0 to 30 min. The default setting is for 5 min. When the UV timer is set to

zero, the UV lamp will not light.

x If the outer door is opened while the UV lamp is lit, the lamp will turn OFF. Then, when the door is

closed, the lamp will light for the preset period of time.

x If the UV lamp ON time is set for longer than 10 minutes or if only the outer door is repeated opened

and closed, condensation may occur in the chamber and may affect temperature distribution. It will also

shorten the service life of the UV lamp.

x When replacing the UV lamp, consult with a Sanyo representative or agent.

Note: To compensate for the drop in UV ray output along with increased accumulated ON time of UV

lamp, the Incubator automatically extends the UV lamp ON time according to the accumulated ON time.

The automatically extended time is displayed as a percentage under UV Timer Ext. in the UV Setting

Screen.

㩷

Lighting the UV lamp for 24 hours

If the chamber has been contaminated by dirt or by spilling the medium, use the following procedure to

decontaminate the chamber by lighting the UV lamp for 24 hours.

㩷

1. Remove all attachments from the chamber, including the trays, tray supports, side supports, fan cover,

duct, fan, humidifying pan, and humidifying pan cover. Clean all the attachments in an autoclave or with

disinfectant alcohol.

2. Clean and wipe off inside the chamber with disinfectant alcohol.

3. Set the CO₂density to 0% and set the UV 24h Mode to 1 on the UV Setting Display.

㩷

U V

S e t

T i m e r

L i

T i m e r

n g

㩷

M E N U

3 0 m i n )

U V

m i

(

O K

C a n c e l

E x t

U V

2 4 h

M o d e

(

0 . O F F

1 . O N )

4. Press the MENU Key to display the menu, select OK, and press the ENTER Key. The parameter will

be saved.

䊶This procedure must be preformed only with the outer door closed and the UV lamp turned OFF.

䊶The UV lamp will light continuously for 24 hours after this parameter is set. The setting is canceled if the

outer door is opened. Perform the above procedure to set the 24-hours lighting mode again if you open

the door.

䊶Install the attachments after completion of the 24-hour lighting mode.

Note: Set the upper limit alarm temperature to at least 10^oC higher than the chamber set temperature

when using the 24-hour lighting mode of UV lamp. The 24-hour lighting mode may cause an automatic

chamber temperature alarm because the temperature of the chamber will increase.㩷

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OTHER PARAMETERS㩷

㩷

Setting the date, time, and log interval

㩷

1. Press the MENU Key from the Top Display to display the menu, select Tools and press the ENTER Key.

㩷

T e m p

3 7 .

C O 2

5 . 0 %

㩷

M E N U

S e t

ꢁꢁ㧡ꢀ㧜ꢁ

㧟㧣ꢀ㧜ꢁ

L o g

T o o l

S t a t u s

O K

2. Select Date Time from the Select Tools Display, press the MENU Key to display the menu, select OK,

and press the ENTER Key.

㩷

S e l e c t

T o o l

C a l

n g

(

1 / 2 )

㩷

T e m p / C O 2

b r a t o n

㩷

M E N U

O K

A l a r m

S e t

L C D / D A Q

S e t

n g

S v c

U V

S e t

L o c k

T i m e

C a n c e l

K e y

D a t

P W

S e t

n g

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㩷

3. The Date Time Display will appear. Set the date, time and log interval.

㩷

D a t

T i m e

M E N U

O K

C a n c e l

㩷

D a t

0 9 / 0 3 / 0 1

1 0 : 0 5 : 0 0

(

Y Y / M M / D D )

T i m e

L o g

h h 㧦 m m 㧦 s s )

n t e r v a l

6 m i

(

2 - 3 0 m i n )

䊶Entering the date

Example for March 1, 2009: Enter 090301 in the Date Field.㩷

䊶Entering the time

Example for 10:05:00: Enter 100500 in the Time Field.

䊶Entering the log interval

Example for 6 minutes: Enter 6 in Log Interval Field.

Note:

Ŷ The default is 6 minutes.

Ŷ The log interval can be set to between 2 and 30 minutes.

Ŷ Relation between the log interval and the period that can be saved

1: Log interval of 2 minutes -- About 5 days

2: Log interval of 6 minutes -- About 14 days

3: Log interval of 30 minutes -- About 70 days

For the data beyond the above period, the older data is deleted to save the newer data.

㩷

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㩷

㩷㩷 Initial settings (LCD/DAQ parameters)

㩷

1. Select LCD/DAQ Setting from the Select Tools Display, press the MENU Key to display the menu,

select OK, and press the ENTER Key.

㩷

S e l e c t

T o o l

C a l

n g

(

1 / 2 )

㩷

T e m p / C O 2

b r a t o n

M E N U

O K

A l a r m

S e t

L C D / D A Q

S e t

n g

S v c

U V

S e t

L o c k

T i m e

C a n c e l

K e y

D a t

P W

S e t

n g

2. The LCD/DAQ Setting Display will appear. Set the initial setting for each parameter as necessary.

㩷

L C D / D A Q

S e t

n g

㩷

M E N U

L C D

D A Q

B a c k

C o l o r

(

1 . B l u e

2 . W h i

O K

e )

S p e e d

0 . 2 4

2 . 9 6

3 . 3 5 0 )

C a n c e l

1 - 2 5 5 )

0 . O F F

0 . L o c a l

M o d e

A l a r m

1 . R e m o t e )

1 . A c t v e )

R e m o t

0 . O F F

䊶LCD Back Color: Setting of the background color (1: Blue, 2: White)

㩷

Note:

DAQ is an external monitoring system of the chamber status. It is necessary to set the DAQ speed, DAQ

ID, and DAQ mode to use the optional communications software. A special order is required for

communications software. Contact a Sanyo sales representative or agent for details.

㩷

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DISPLAYING THE LOG㩷

㩷

A log of the past chamber temperatures and CO₂densities can be displayed on a graph.

㩷

1. Press the MENU Key from the Top Display to display the menu, select Log and press the ENTER Key.

㩷

T e m p

3 7 .

C O 2

5 . 0 %

㩷

M E N U

S e t

ꢁꢁ㧡ꢀ㧜ꢁ

㧟㧣ꢀ㧜ꢁ

L o g

T o o l

S t a t u s

O K

2. The log will be displayed with dots. Press the Up Cursor Key and Down Cursor Key to switch between

the temperature and CO₂density displays. Press the Left Cursor Key and Right Cursor Key to scroll the

displayed data (Left Cursor Key: older data, Right Cursor Key: newer data).

㩷

Temperature

㩷

5 0 ° C

2 0 0 9 / 0 3 / 0 1

T e m p

㩷

1 2

2 4

㪪㫎㫀㫋㪺㪿㪼㪻㩷㫎㫀㫋㪿㩷㪬㫇㩷㪸㫅㪻㩷㪛㫆㫎㫅㩷㪚㫌㫉㫊㫆㫉㩷㪢㪼㫐㫊

CO₂density

2 0 %

2 0 0 9 / 0 3 / 0 1

C O 2

1 2

2 4

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㩷

Transferring data

㩷

Use the following procedure to transfer the log data to a PC.㩷㩷

㩷

1. To transfer the log data for one day, press the MENU Key to display the menu, select PC 1D, and

press the ENTER Key. To transfer all of the log data, select PC All and press the ENTER Key.

㩷

5 0 ° C

2 0 0 9 / 0 3 / 0 1

T e m p

M E N U

㩷

P C

1 D

A l

C l e a r

C a n c e l

1 2

2 4

2. A Progress Display will appear. On a HyperTerminal on PC, specify a transfer, text capture, and the

name of the file to save. Use TXT or CSY as the file name extension. Press the MENU Key to display the

menu, select Start, and press the ENTER Key.

The transfer will be started. “Finished” will be displayed when the transfer has been completed. Select

Cancel from the menu after the transfer has been completed and press the ENTER Key.

㩷

P r o g r e s s

M E N U

S t a r t

C a n c e l

㩷

S e n d

L o g

o g

d a t

P C .

D a t

2 0 0 9 / 0 3 / 0 1

F i n i s h e d .

㩷

Setting in PC side for transmission of log data (For Windows 2000 and Windows XP)

1. From the Windows Start Button, select Program㩷㪄 Accessories㩷㪄 Communications㩷㪄

HyperTerminal to start the HyperTerminal. (If the HyperTerminal is not available from the Start Menu,

execute the following file: C:¥Program Files¥Windows NT¥hypertrm.exe.)

2. In the HyperTerminal Window, set a new connection, the name (for example, Sanyo), connection

settings, method of connection, COM1, properties of COM1, and port.

Baud rate: 9,600, Data bits: 8, Parity: None, Stop bits: 1, Flow control: Xon/Xoff.

(The communications condition of the Incubator will be set automatically to the above settings when the

Progress Display appears.)

Note: An optional communication terminal, MTR-480(RS-232C/RS-485) or MTR-L03(LAN/RS-485) is

required to transfer data.

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WATER LEVEL SENSOR

This Incubator is equipped with a water level sensor for the humidifying pan. The sensor is set

automatically when the humidifying pan is installed. Take care not to damage the sensor when removing

or installing the humidifying pan.

When the humidifying pan is removed (side view)

Sensor

Pan

When the humidifying pan is installed (side view)

Sensor

Pan

Note: Make sure that the humidifying pan is all the way to the back and that the sensor comes down

when you install the humidifying pan.

Note:

• Lift the sensor before installing the humidifying pan if the sensor is in the lower position after

maintenance.

• When installing the humidifying pan, make sure that the pan is set properly and that sensor comes down

into the pan. “RH PAN” will be displayed in reverse video in the status display field on LCD panel if the

sensor does not come down completely. If necessary, set the pan again in the proper location.

• The sensor detects the water level every 30 minutes and just after the outer door is closed. It takes

several seconds to detect the water level. Therefore, “RH PAN” may displayed in reverse video several

times in the status display field on LCD panel after the outer door is closed even when the humidifying pan

is full.

CAUTION

Foreign particles on the water surface can adhere to the water level sensor and fittings by capillary action

because the sensor is always in the water. The adhered foreign particles degrade sensor performance

and “RH PAN” may displayed in reverse video in the status display field on LCD panel even though there

is sufficient water in the humidifying pan. Be sure to wipe OFF any dirt on the water level sensor with

disinfectant alcohol whenever you change the humidifying water. When cleaning the sensor, take care

not to apply excessive force to the lead wires.

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ROUTINE MAINTENANCE

Cleaning the chamber and Inner attachments

When using the Incubator for the first time or when performing H₂O₂decontamination, refer to Removing

Chamber Attachments on the following page and remove and clean the inner attachments.

WARNING

Before performing any repairs or maintenance, turn OFF the power supply switch and unplug the

Incubator. Failure to do say may result in electric shock or injury.

Be careful not to inhale chemicals, vapors or aerosols when cleaning the Incubator. Doing so may

be harmful to health.

CAUTION

Wear rubber gloves when performing maintenance on the chamber. Failure to wear gloves may

result in cuts or abrasions from sharp edges or corners.

Note:

Be careful not to damage the humidifying pan water level sensor or the UV lamp in the chamber duct (if

the MCO-19AIC(UV) or the optional MCO-19UVS is installed).

Do not use detergents or antiseptic solutions with acid, alkali, or chlorine. Doing so may cause

discoloration, corrosion, or rusting.

1. Turn OFF the power to the Incubator.

2. Open the outer and inner doors and pull out the trays. (See Fig. 1.)

3. Lift the front of the tray supports and pull them out. (See Fig. 2.)

4. Lift the humidifying pan cover off from the pins on the rear side. (See Fig. 3.)

Fig. 1

Fig. 2

Fig. 3

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5. Pull out the humidifying pan. (See Fig. 4.)

6. Loosen the two screws securing the fan cover and take off the fan cover. (See Fig. 5.)

7. Lift the duct and remove it from the pins on the rear side. (See Fig. 6.)

Fig. 4

Fig. 5

Fig. 6

8. Remove the chamber circulation fan by pulling out the central spring and then by pulling out the fan.

(See Fig. 7.)

9. Remove the screw securing the clamp for the tray brace. (See Fig. 8.)

10. Remove the clamp. (See Fig. 9.)

11. Lift the tray brace off of the pins. (See Fig. 10.)

Fig. 7

Fig. 8

Fig. 9

128

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12. Clean all the attachments with a diluted neutral detergent, and then rinse them thoroughly with

distilled water.

13. Wipe the trays, the inner attachments such as the chamber circulation fan, and the chamber sides

with sterilizing alcohol. Be careful not to leave any residual alcohol.

14. Wipe the water level sensor with sterilizing alcohol. Be careful not to leave any residual alcohol. When

cleaning the sensor, take care not to apply excessive force to the lead wires.

15. To reinstall all the attachments, perform the procedure in reverse order from step 14.

Note:

• When installing the chamber circulation fan, insert

it securely on the motor shaft. Lightly turn the fan

manually to make sure that it does not strike the rear

panel. Improper insertion may cause poor

performance.

• As shown in Fig. 11, set the tray with only the front

edge bent down. If the tray is set in the wrong

direction, it may not be level and may become

Bent down

unstable.

Fig. 11

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Filling the humidifying pan

Use the following procedure to fill the humidifying pan or to replace the water.

1. Lift the humidifying pan cover. (See Fig. 1.)

2. Pull the humidifying pan forward. (See Fig. 2.)

3. Dispose of the remaining water in the pan and

clean the pan with a neutral dishwashing detergent.

Then rinse it thoroughly with distilled water and wipe

it with sterilizing alcohol.

4. Wipe all moisture from the bottom of the chamber.

Fig. 1

5. Return the pan to the chamber and add sterile

distilled water (approx. 1.5 L, preheated to 37qC).

(See Fig. 3.)

6. Set the pan with the inner side flush against the

back, and replace the cover. Close the inner and

outer doors, and confirm that RH PAN is not

displayed in reverse video in the status display area.

Note:

Fig. 2

• Preheat to 37qC the water to be added to the

humidifying pan. Adding low-temperature water will

lower the temperature and humidity in the chamber.

• If the water level alarm indication “RH PAN” is

displayed, use the above procedure to replenish the

water.

CAUTION

When refilling the water in the humidifying pan,

always wipe off any dirt from the water level

sensor with sterilizing alcohol. While doing that,

be careful not to apply excessive force to the

sensor lead wire.

Fig. 3

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H₂O₂DECONTAMINATION

㩷

When the chamber has been contaminated, or when cleaning the chamber prior to starting a culture,

H₂O₂decontamination can be performed using the H₂O₂Generator.

z The following products must be purchased separately in order to perform H₂O₂decontamination. Before

performing H₂O₂decontamination, check to make sure that these are correctly installed.

x UV Lamp Expansion Kit (MCO-19UVS) (Not required for the MCO-19AIC(UV).)

x H₂O₂Decontamination Kit (MCO-HL)

x H₂O₂Generator (MCO-HP)

z The H₂O₂decontamination function decontaminates the chamber and the inner attachments. Do not

place any objects other than the specified attachments in the chamber during decontamination.

z Before performing H₂O₂decontamination, clean the chamber and the inner attachments with sterilizing

alcohol.

z The chamber temperature during H₂O₂decontamination reaches 45qC. Before performing H₂O₂

decontamination, set the upper limit temperature alarm to 50qC or higher.

z For the H₂O₂reagent, use one bottle of the H₂O₂Decontamination Reagent specified by Sanyo

(MCO-H202).

z After decontamination, dilute the residual H₂O₂Decontamination Reagent in the H₂O₂Generator with a

large quantity of water and dispose of it, and rinse with distilled water. (Do not wash either the inside or

outside of the H₂O₂Generator with alcohol.)

WARNING

Use the reagent specified by Sanyo for H₂O₂decontamination. Using a different H₂O₂solution may

cause explosion or damage to the Incubator, or insufficient decontamination.

Do not use chemicals other than the H₂O₂reagent, such as alcohol. Doing so may result in damage

to the H₂O₂Generator.

WARNING

When performing H₂O₂decontamination, make sure that the outer and inner doors are securely

closed. During H₂O₂decontamination, plug the access hole with the silicon cap that is provided. Failure to

do so may be harmful to health due to leakage of H₂O₂gas.

CAUTION

H₂O₂decontamination can be performed only for the chamber and chamber attachments with standard

specifications, and not for any other objects.

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CAUTION

Perform H₂O₂decontamination with the chamber attachments arranged as specified by Sanyo.

Arranging them in a different way may result in insufficient decontamination.

CAUTION

Wear rubber gloves when handling the H₂O₂reagent. Direct contact with the H₂O₂reagent may result

in inflammation of the skin.

CAUTION

After H₂O₂decontamination has been completed, residual H₂O₂solution will remain on the bottom of the

chamber, the H₂O₂Generator, and the bottom of the duct. Wearing protective glasses and rubber

gloves, wipe it off with a non-woven cloth. Failure to do so may result in a deficient culture.

㩷

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H₂O₂decontamination

Use the following procedure to perform H₂O₂decontamination using the H₂O₂Generator (MCO-HP).

1. Take out all trays, tray supports, side supports, the humidifying pan cover, the humidifying pan, the fan

cover, and the duct from the chamber. Dispose of the water in the humidifying pan, wipe the inside walls

of the chamber with a gauze containing water or alcohol for sterilization.

2. Attach the duct and side supports, and set the tray supports in the 2nd, 5th, 7th, 9th holes from the top

of the side supports. Then set the trays.

3. Pour one bottle of H₂O₂Decontamination Reagent (MCO-H2O2) into the H₂O₂Generator. Set the two

pins on the H₂O₂Generator in the 2 holes on the lower left side of the duct (Fig. 1).

4. Connect the H₂O₂Generator and connector on the bottom right of the far side of the chamber with the

enclosed cable. Be sure to keep the connector cap (Fig. 1).

5. Set the humidifying pan cover, humidifying pan, and fan cover as shown in Fig. 2, and close the inner

door and outer door (Fig. 2).

Note: The trays included as accessory are designed to be appropriate for decontamination. If Half Trays

(MCO-25ST, optional) or Trays for previous models are used, decontamination may not be sufficiently

effective.

Front

UV lamp

Connector

H₂O₂Generator

Ultrasound oscillator

Fig. 1

Humidifying pan cover

Front

Fan cover

Humidifying pan

H₂O₂

Generator

Fig. 2

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6. Press the H₂O₂Key for 5 seconds. The system check will start.

H 2 O 2

D e c o n t a m i n a t

o n

M E N U

O K

C a n c e l

H 2 O 2

D e c o n

S y s t e m

C h e c k

࡮

7. If the system is normal, the following display will appear. (If the system is not normal, refer to Table 2

on page 47.) Select OK and press the ENTER Key to start H₂O₂decontamination. Decontamination will

be performed automatically to step 10. The buzzer will sound when decontamination has been completed

(It takes about 1 hour 40 minutes after warming-up).

Note: The outer door will be locked with an electric lock for safety until the decontamination has been

completed.

H 2 O 2

D e c o n t a m i n a t

o n

M E N U

O K

C a n c e l

R e a d y

S t a r t

8. During the H₂O₂mist generation, “H2O2 Decon” will flash at the top left corner of the screen.

Note: H₂O₂mist generates from the H₂O₂Generator and then quickly changes to a gas.

H 2 O 2

D e c o

U V

R e s o l v e

S t e p :

ꢀ㧥㧜OKPꢀ

㧣OKPꢀ

T e m p : 4 5 . 0 㷄㩷

D o o r : L o c k e d

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9. After completion of H₂O₂mist generation, “UV Resolve” will flash at the top of the screen, and H₂O₂gas

resolution by UV light will start.

H 2 O 2

D e c o

U V

R e s o l v e

S t e p :

ꢀ㧤㧥OKPꢀ

ꢀꢀ㧜OKPꢀ

T e m p : 4 5 . 0 㷄㩷

D o o r : L o c k e d

10. After H₂O₂gas resolution, H₂O₂decontamination has been completed. The following display will

appear. Open the outer door and disconnect the cable of H₂O₂Generator from the connector in the

chamber. Then take out the H₂O₂Generator.

Note: Always put on protection glasses and rubber gloves when performing decontamination.

H 2 O 2

D e c o n t a m i n a t

o n

S t e p :

D e c o n

F i n i s h e d .

T e m p : 4 5 . 0 㷄㩷

D o o r : U n l o c k

P l e a s e

R e m o v e

H 2 O 2

U n

11. The following display will appear. Press the MENU Key to display the menu, select OK, and press the

ENTER Key to return to the Top Display.

H 2 O 2

D e c o n t a m i n a t

o n

S t e p :

D e c o n

F i n i s h e d .

T e m p : 4 5 . 0 㷄㩷

D o o r : U n l o c k

P U S H

M E N U

O K

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12. Dilute the remaining H₂O₂reagent in the H₂O₂Generator with a large volume of water and dispose of

it. Rinse and wash the H₂O₂Generator with distilled water. Then keep the H₂O₂Generator in a clean

environment outside of the chamber.

Note: After H₂O₂decontamination, cover the connector on the chamber side with the connector cap.

13. After H₂O₂decontamination, surplus H₂O₂liquid will remain at the bottom of the chamber and in the

bottom part of the H₂O₂Generator duct. This solution contains H₂O₂at a low concentration (about 1% or

less), so put on protective glasses and rubber gloves and wipe it up with a non-woven cloth.

14. Ventilate the chamber sufficiently and place all the attachments back into the chamber.

Note: Be sure to attach the connector cap.

_㩷CAUTION

The electric lock will remained locked if power fails during H₂O₂decontamination. After the power comes

back ON, the H₂O₂gas resolution process will start execution and finish automatically. Execute the

decontamination again because the decontamination will be incomplete.

WARNING

Never open the door by unlocking it with the unlock key during H₂O₂decontamination or H₂O₂gas

resolution with the UV lamp. H₂O₂gas leakage is potentially harmful to health.

Precautions when handling H₂O₂decontamination reagent

Observe the following precautions when handling the H₂O₂Decontamination Reagent (MCO-H2O2).㩷

㩷

z Handling Precautions

x Wear protective equipment, such as protective glasses and rubber gloves.

x Do not use fire in the area where the reagent is being handled.

x Do not leave any reagent in the container after it has been used or while it is being used.

x Do not place inflammable or combustible materials near the area where the reagent is handled.

z Precautions for Storage

x Always close the container cover securely to prevent impurities from becoming mixed in the reagent.

x Check the container to make sure that there is no damage, corrosion, or cracking.

x Store the container with the inlet facing upwards, and make sure that the container will not tip or be

knocked over.

x If possible, store the reagent in a location where water is available and where the floor can be easily

washed.

z Precautions for Disposal

x Dispose according to the rule in your country.

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ALARMS, SAFETY, AND SELF-DIAGNOSIS

The Incubator supports the following alarms, safety functions, and self-diagnostic functions.

Table 1: Alarms and Safety Functions for Culture Operations

Alarm or safety

Conditions

Display

Buzzer

Safety operation

function

Upper limit

temperature

alarm

Automatic set

temperature

alarm

The chamber temperature exceeds the Err13: Main Heater Abnormal or Err16: Continuous tone

upper limit alarm temperature set value. CO₂S Box Heater Abnormal is

displayed in the message display field.

Heater OFF

Remote alarm

operates

The chamber temperature is out of the Warning :High Temp or

Intermittent tone

after set alarm delay operates after delay

time (0 to 15 min)

has elapsed

Intermittent tone

Remote alarm

automatic set temperature alarm setting Warning :Low Temp

range ( 1.0 to 5.0^oC).

is displayed in the message display

field.

The chamber CO₂concentration is out Warning :High CO₂Density or

of 0 to 15 min.

Automatic set

CO₂

Remote alarm

of the automatic set CO₂concentration Warning :Low CO₂Density is displayed after set alarm delay operates after delay

alarm setting range ( 0.5% to 5.0%). in the message display field.

concentration

alarm

time (0 to 15 min)

has elapsed

-----

of 0 to 15 min.

Auto-return

There are no key operations for approx. Top screen is displayed.

90 s in a setting mode.

The setting mode is

canceled.

Key lock

The key lock is ON.

Key Lock is displayed on the standby

operation setting screen.

-----

Setting changes are

prohibited.

Door alarm

The outer door is open.

Door䋺Open is displayed in the

message display field.

Intermittent tone

after set alarm delay closed.

The CO₂valve is

time (1 to 30 min)

has elapsed

Err01: CO₂_㩷Gas Empty is displayed in Intermittent tone

The heater turns

OFF after 1 min.

Remote alarm

operates.

CO₂gas

The CO₂concentration does not

cylinder empty increase when the CO₂valve is

opened.

the message display field.

CO₂line

The CO₂gas supply is switched

automatically to the reserve cylinder

(only when the MCO-21GC is installed). The text flashes for the empty CO₂gas

supply line.

The chamber temperature sensor is

disconnected.

Err01: CO₂_㩷Gas Empty is displayed in

the message display field.

䇰

The CO₂gas supply

is switched.

Remote alarm

operates.

The heater turns

OFF.

Remote alarm

operates.

The heater turns

OFF.

changeover

Chamber

temperature

sensor error

Err05䋺Temp Sensor Open

is displayed in the message display

field.

The chamber temperature sensor is

short-circuited.

Err06䋺Temp Sensor Short

is displayed in the message display

field.

Remote alarm

operates.

Sensor box

temperature

sensor error

The sensor box temperature sensor is Err07: CO₂S Box Temp Sensor Open

䇰

CO₂valve is closed.

Remote alarm

operates.

CO₂valve is closed.

Remote alarm

operates.

disconnected.

is displayed in the message display

field.

The sensor box temperature sensor is Err08䋺CO₂S Box Temp Sensor Short is

short-circuited.

displayed in the message display field.

Ambient

The ambient temperature sensor is

disconnected.

Err09: At Sensor Open is displayed in

the message display field.

䇰

Remote alarm

operates.

temperature

sensor error

The ambient temperature sensor is

short-circuited.

Err10: At Sensor Short is displayed in

the message display field.

Remote alarm

operates.

CO₂sensor

error

The Vref or Vgas output voltage for the Err11: CO₂_㩷Sensor Vref Abnormal or

CO₂sensor is abnormal.

CO₂valve is closed.

Remote alarm

operates.

Error12: CO₂_㩷Sensor Gas Abnormal is

displayed in the message display field.

Main heater

error

Main heater burnout occurs or the main Err13: Main Heater Abnormal is

heater SSR is short-circuited when the displayed in the message display field.

upper limit temperature alarm operates.

䇰

Remote alarm

operates.

Bottom heater

error

Bottom heater burnout occurs or the

bottom heater SSR is short-circuited.

Err14: Humidity Heater Abnormal

is displayed in the message display

field.

Remote alarm

operates.

Door heater

error

Door heater burnout occurs or the door Err15: Door Heater Abnormal is

䇰

Remote alarm

operates.

heater SSR is short-circuited.

displayed in the message display field.

Sensor box

heater error

Sensor box heater burnout occurs or

the sensor box heater SSR is

short-circuited.

Err16: CO₂S Box Heater Abnormal is

displayed in the message display field.

Remote alarm

operates.

Heater SSR

burnout

Main, bottom, door, or sensor box

heater SSR burnout occurs.

Err17: SSR Open is displayed in the

message display field.

䇰

Remote alarm

operates.

-----

Low humidifying The water in the humidifying pan drops RH PAN flashes in reverse video in the

water to approx. 600 ml. status display field.

UV lamp failure The UV lamp burns out (only for the Err18: UV Lamp Abnormal is displayed Intermittent tone

MCO-19AIC(UV) or when the optional in the message display field.

-----

Remote alarm

operates.

MCO-19UVS is installed).

New UV lamp

The accumulated ON time reaches

UV flashes (when the UV lamp is not

lit) in the status display field.

-----

recommended approx. 1,000 hours (only for the

MCO-19AIC(UV) or when the optional

MCO-19UVS is installed).

137

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㩷

ꢀ The buzzer can be stopped by pressing the BUZZER Key, but remote alarms will not stop. In addition, a

buzzer that sounds because of the upper limit temperature alarm cannot be stopped by pressing the

BUZZER Key.

ꢀ Err01 recovery is achieved automatically by correctly connecting the gas and pressing the BUZZER Key

to stop the buzzer. In addition, when the MCO-21GC is installed and the gas supply is switched to the

reserve gas cylinder, press the BUZZER Key and replace the gas cylinder.

ꢀ If an error from Err05 to Err18 occurs (except for the upper limit temperature alarm in Err13 to Err16),

then consult a Sanyo sales representative or agent.

Table 2: Alarm and Safety Functions for H₂O₂Decontamination

Alarm and safety

function

Condition

Display

Buzzer

Safety operation

System check error The H₂O₂Generator is not

H2O2 Decon System Error is displayed

in the screen center.

Err31:H2O2 Unit No Connect is

displayed in the message display field.

H2O2 Decon System Error is displayed

in the screen center.

-----

Decontamination mode is

cancelled.

at start of H₂O₂

decontamination㩷

connected.

There is no H₂O₂solution or

the H₂O₂level sensor has

failed (or is disconnected).

-----

Decontamination mode is

cancelled.

Err32: H2O2 Level is Low is displayed in

the message display field.

A door is not closed.

H2O2 Decon System Error displayed in

the screen center.

Decontamination mode is

cancelled.

Err33: Door is Open is displayed in the

message display field.

Failure during H₂O₂The volume of H₂O₂generated Err34:H2O2 Volume is displayed in the Intermittent Moves to UV analysis.

decontamination㩷 is abnormal. message display field. tone Remote alarm operates.

Power was interrupted during After power is restored, Err35: Power Intermittent Electrically locked during power

H₂O₂decontamination.

Failed is displayed in the screen

message display field.

After completion, Decon Failed is

displayed in the screen center.

tone

interruption. After power is

restored, moves to UV analysis.

Remote alarm operates.

Failure during H₂O₂The UV lamp failed during

gas analysis by UV H₂O₂gas analysis by UV.

Err36: UV Lamp Failure is displayed in Intermittent UV analysis time is extended.

the message display field.

tone

Remote alarm operates.

Power was interrupted during After power is restored, Err37: Power

-----

Electrically locked during power

interruption. After power is

restored, UV analysis is

repeated.

H₂O₂gas analysis by UV.

Failed is displayed in the message

display field.

Table 3: Alarm and Safety Functions for Automatic Standard Gas Calibration

Alarm and safety

function

Condition

Display

Buzzer

Safety operation

System check error The gas pressure for the

Std Gas Calib Error is displayed in the

screen center.

Err41: CO2 Std Gas Empty is displayed

in the message display field.

Std Gas Calib Error is displayed in the

screen center.

Intermittent The standard gas calibration

tone

at start of automatic standard gas calibration CO₂

mode is cancelled.

Remote alarm operates.

standard gas

calibration

Failure during

gas line is less than the

specified pressure.

The gas pressure for the

Intermittent Automatic calibration is

tone

automatic standard standard gas calibration CO₂

cancelled.

Remote alarm operates.

gas calibration㩷

gas line is less than the

specified pressure.

Err42: CO2 Std Gas Empty is displayed

in the message display field.

Note䋺

For models with electric locking, an unlock key is provided in order to unlock the door during a power

interruption or in case the electric lock fails. Always store this key in a safe place. It is recommended that

you make a note of the key symbol and number in case the key is lost.

WARNING

Do not use the unlock key to unlock the door during H₂O₂decontamination or during H₂O₂gas

analysis by UV. Doing so may cause harm to health from H₂O₂gas leakage.

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ꢀ CALIBRATION

Temperature/CO₂calibration

1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTER

Key.

㩷

T e m p

3 7 .

C O 2

5 . 0 %

㩷

M E N U

S e t

ꢀꢀ㧡ꢁ㧜ꢀ

㧟㧣ꢁ㧜ꢀ

L o g

T o o l

S t a t u s

O K

2. Select Temp/CO2 Calibration from the Select Tools Display, press the MENU Key to display the

menu, select OK, and press the ENTER Key.

㩷

S e l e c t

T o o l

C a l

n g

(

1 / 2 )

㩷

M E N U

O K

T e m p / C O 2

b r a t o n

A l a r m

S e t

L C D / D A Q

S e t

n g

S v c

U V

S e t

L o c k

T i m e

C a n c e l

K e y

D a t

P W

S e t

n g

3. The Temp/CO2 Calibration Display will appear.

㩷

Example

Enter the value shown below if the displayed chamber temperature is 37.0^oC but the actual temperature

is 36.5^oC.

ƔTemperature span: Enter 365.

㩷

T e m p / C O 2

C a l

b r a t

o n

P V

V o l

T e m p

C O 2

S p a n

Z e r o

S p a n

R e f

3 6 .

3 7 .

0 . 0 ° C

5 0 0

0 . 1 %

0 . 0 %

5 . 0 %

4 . 0 2

4 . 0 1

4 . 0 2

4 . 0 1

C O 2

5 0 0

C O 2

G a s

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ꢀ

Example

Enter the value shown below if the displayed chamber CO₂density is 5.0% but the actual CO₂density is

4.5%.

ƔCO₂density span: Enter 045.㩷

T e m p / C O 2

C a l

b r a t

o n

P V

7 .

V o l

T e m p

C O 2

S p a n

Z e r o

S p a n

R e f

0 .

0 . 0 ° C

5 0 0

0 . 0 %

4 . 5 %

4 . 0 2

4 . 0 1

4 . 0 2

4 . 0 1

C O 2

5 . 0 %

5 0 0

C O 2

G a s

4. Press the MENU Key to display the menu, select OK, and press the ENTER Key.

5. After the Select Tools Display is displayed, press MENU Key to display the menu, select Cancel, and

press the ENTER Key to return to the Top Display.

Note:

It is important to accurately measure the temperature inside the Incubator when calibrating the

temperature. Particularly, the temperature gauge used must have an accuracy of JIS 0.5 Class or better.

The temperature must be measured at several points.

The temperature setting must not change by more than r1.0^oC, and the CO₂density must not change

by more than r1.0% during calibration. If these values are exceeded, an error buzzer will sound, the

input data will be ignored, and the Incubator will return to the display mode. If it is necessary to change

the temperature by more than 1.0^oC or the CO₂density by more than 1.0%, perform calibration in several

stages over a period of time.

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ꢀ TROUBLESHOOTING

If the Incubator does not seem to be working properly, check the following items before calling for service.

Symptom

Items to check and countermeasures

x Is the Incubator plugged in?

The Incubator does not

operate at all.

x Is there a power outage, or has a circuit breaker interrupted the

power?

x Is the key lock function turned OFF?

The key is not working.

An alarm has occurred.

When starting operation:

x Does the chamber temperature match the set value?

x Does the CO₂gas concentration in the chamber match the set value?

(1) Is the secondary pressure for the CO₂gas pressure regulator at the

specified value of 0.03 MPaG (0.3 kgf/cm²G)?

(2) Is the CO₂gas supply pipe properly connected?

During operation:

x Is the upper limit alarm temperature set at least 1qC higher than the

chamber set temperature?

x Has the temperature setting been changed? Has the door been left

open for a long time? Has a low-temperature object been placed in the

chamber? If any of these is the case, the alarm will be automatically

cleared if you wait.

x Has the CO₂gas supply pipe come loose, or is there a gas leak?

x Has the CO₂gas concentration setting been changed?

x Is the gas cylinder empty? Check the primary pressure of the gas

cylinder once a week. (When the primary pressure is 3.8 MPa (38

kgf/cm²G) or lower, it is a sign that there is little gas remaining.

Replace the cylinder soon.)

x Is the ambient temperature less than 5qC different from the set value

for the chamber temperature?

The chamber temperature

does not match the set value.

x Is the outer door closed with the inner door left open?

x Is the secondary pressure for the CO₂gas pressure regulator at the

The CO₂gas concentration

does not match the set value.

specified value of 0.03 MPaG (0.3 kgf/cm²G)?

x Is the CO₂gas supply pipe blocked?

x Is there enough water in the humidifying pan? (Be sure to use sterile

distilled water.)

The chamber temperature

does not rise.

x Are the doors being frequently opened and closed?

A large quantity of CO₂gas is

being consumed.

x Check whether gas is leaking from connectors due to deterioration of

the CO₂gas supply pipe, or whether there may be any pinhole leaks.

The CO₂gas supply pipe is a replaceable part, and it is recommended

that it be replaced once a year.

x Is the packing seal for the inner door defective?

x Is the access hole open?

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ꢀ

Symptom

Items to check and countermeasures

x Is the ambient air environment around the Incubator normal? Is

there a source of polluted gas in the vicinity?

Normal cultures are not possible,

and the CO₂gas concentration

is suspect.

x A HEPA filter is used for the Incubator CO₂gas piping. If gas

concentration is slow to recover when the CO₂gas concentration is

normal, it is possible that the HEPA filter may be clogged. Consult a

Sanyo representative or agent.

The CO₂gas concentration is

slow to recover.

x Is there little gas remaining in the CO₂gas cylinder?

x Is the secondary pressure for the CO₂gas pressure regulator at the

specified value of 0.03 MPaG (0.3 kgf/cm²G)?

x Is the CO₂gas supply pipe blocked?

x The CO₂control method for the Incubator is the ON-OFF method.

CO₂gas is intermittently injected as the gas concentration in the

chamber approaches the set value. Injections may be stopped for

periods of approximately 15 seconds, but that is not an error.

CO₂gas is not being injected.

The outer door does not open.

When the optional MCO-HL is installed:

x When the power is OFF, the electric lock is locked and the outer

door does not open. Either turn ON the power or use the accessory

unlock key to override the electric lock.

x During decontamination the outer door is electrically locked and

does not open.

x If the MCO-19AIC(UV)/19AIC is being used, is the optional

MCO-HL installed?

H₂O₂decontamination cannot be

performed.

x If the MCO-19AIC is being used, are the optional MCO-HL and

MCO-19UVS installed?

x Is the UV lamp burned out? If the UV lamp is burned out, H₂O₂

decontamination will not be possible.

x Is the H₂O₂Generator cable properly connected?

x Has the entire bottle of H₂O₂Decontamination Reagent been used?

x Has the upper limit temperature alarm been set to 50qC or higher?

Note: If the problem still has not been solved after trying the above checks and countermeasures, or for

any problems not covered here, contact a Sanyo representative or agent.

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DISPOSING OF THE CO₂INCUBATOR

WARNING

If the Incubator is to be stored unused for a period of time, ensure that children do not have access to

the Incubator and that the Incubator doors cannot be completely closed. If the doors can be closed,

it may be possible for children to become locked inside.

The Incubator must be dismantled and disposed of by qualified personnel only. If the doors are not

removed, it may be possible for children to become locked inside.

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Note:

This symbol mark and recycle system apply only to EU countries. They

do not applied to the countries in other areas of the world.

Waste Electrical and Electronic Equipment (WEEE) Directive 2002/96/EC

(English)

This SANYO product is designed and manufactured with high-quality materials and components that can

be recycled and reused.

This symbol means that the electrical and electronic equipment, at their end-of-life, should be disposed of

separately from household wastes.

Dispose of this product at your local community waste collection/recycling center.

In the European Union there are separate collection systems for used electrical and electronic products.

Please help us to conserve the environment we live in.

(German)

Ihr SANYO Produkt wurde entworfen und hergestellt mit qualitativ hochwertigen Materialien und

Komponenten, die recycelt und wiederverwendet werden können.

Dieses Symbol bedeutet, daß elektrische und elektronische Geräte am Ende ihrer Nutzungsdauer von

Hausmüll getrennt entsorgt werden sollen.

Bitte entsorgen Sie dieses Gerät bei Ihrer örtlichen kommunalen Sammelstelle oder im Recycling Centre.

In der Europäischen Union gibt es unterschiedliche Sammelsysteme für Elektrik- und Elektronikgeräte.

Helfen Sie uns bitte, die Umwelt zu erhalten, in der wir leben!

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(French)

Votre produit Sanyo est conçu et fabriqué avec des matèriels et des composants de qualité supérieure qui

peuvent être recyclés et réutilisés.

Ce symbole signifie que les équipements électriques et électroniques en fin de vie doivent être éliminés

séparément des ordures ménagères.

Nous vous prions donc de confier cet équipement à votre centre local de collecte/recyclage.

Dans l’Union Européenne, il existe des systèmes sélectifs de collecte pour les produits électriques et

électroniques usagés.

Aidez-nous à conserver l’environnement dans lequel nous vivons !

Les machines ou appareils électriques et électroniques contiennent fréquemment des matières qui, si elles

sont traitées ou éliminées de manière inappropriée, peuvent s’avérer potentiellement dangereuses pour la

santé humaine et pour l’environnement.

Cependant, ces matières sont nécessaires au bon fonctionnement de votre appareil ou de votre machine.

Pour cette raison, il vous est demandé de ne pas vous débarrasser de votre appareil ou machine usagé

avec vos ordures ménagères.

(Spanish)

Los productos SANYO están diseñados y fabricados con materiales y componentes de alta calidad, que

pueden ser reciclados y reutilizados.

Este símbolo significa que el equipo eléctrico y electrónico, al final de su ciclo de vida, no se debe

desechar con el resto de residuos domésticos.

Por favor, deposite su viejo “televisor” en el punto de recogida de residuos o contacte con su

administración local.

En la Unión Europea existen sistemas de recogida específicos para residuos de aparatos eléctricos y

electrónicos.

Por favor, ayúdenos a conservar el medio ambiente!

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(Portuguese)

O seu produto SANYO foi concebido e produzido com materiais e componentes de alta qualidade que

podem ser reciclados e reutilizados.

Este símbolo significa que o equipamento eléctrico e electrónico no final da sua vida útil deverá ser

descartado separadamente do seu lixo doméstico.

Por favor, entregue este equipamento no seu ponto local de recolha/reciclagem.

Na União Europeia existem sistemas de recolha separados para produtos eléctricos e electrónicos

usados.

Por favor, ajude-nos a conservar o ambiente em que vivemos!

(Italian)

Il vostro prodotto SANYO è stato costruito da materiali e componenti di alta qualità, che sono riutilizzabili o

riciclabili.

Prodotti elettrici ed elettronici portando questo simbolo alla fine dell’uso devono essere smaltiti

separatamente dai rifiuti casalinghi.

Vi preghiamo di smaltire questo apparecchio al deposito comunale.

Nell’Unione Europea esistono sistemi di raccolta differenziata per prodotti elettrici ed elettronici.

Aiutateci a conservare l’ambiente in cui viviamo!

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(Dutch)

Sanyo producten zijn ontwikkeld en gefabriceerd uit eerste kwaliteit materialen, de onderdelen kunnen

worden gerecycled en weer worden gebruikt.

Het symbool betekent dat de elektrische en elektronische onderdelen wanneer deze vernietigd gaan

worden , dit separaat gebeurt van het normale huisafval.

Zorg ervoor dat het verwijderen van de apparatuur bij de lokaal erkende instanties gaat gebeuren.

In de Europese Unie wordt de gebruikte elektrische en elektronische apparatuur bij de daarvoor wettelijke

instanties aangeboden.

Alstublieft help allen mee om het milieu te beschermen.

(Swedish)

Din SANYO produkt är designad och tillverkad av material och komponenter med hög kvalitet som kan

återvinnas och återanvändas.

Denna symbol betyder att elektriska och elektroniska produkter, efter slutanvändande, skall sorteras och

lämnas separat från Ditt hushållsavfall.

Vänligen, lämna denna produkt hos Din lokala mottagningstation för avfall/återvinningsstation.

Inom den Europeiska Unionen finns det separata återvinningssystem för begagnade elektriska och

elektroniska produkter.

Vänligen, hjälp oss att bevara miljön vi lever i!

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AUTOMATIC CO₂CYLINDER CHANGEOVER

An automatic switcher for the CO₂gas supply lines (MCO-21GC) is available as an optional accessory.

This kit switches the gas supply lines when one CO₂gas cylinder becomes empty.

Note: The installation of the MCO-21GC must be performed only by qualified service personnel.

Install the MCO-21GC and then perform the following procedure

㩷

1. Connect the CO₂gas pipes to ports A and B. Connecting ports for CO₂gas pipes are located on the

left back of the Incubator. (Refer to page 8).㩷㩷

Note: Only port A can be used when the MCO-21GC is not installed. There will be 2 ports, ports A and B,

after the MCO-21GC is installed.㩷

㩷

2. Connect a CO₂gas cylinder equipped with a gas pressure regulator to each gas pipe. Refer to page 14

for information on connecting the gas cylinders. (“Gas cylinder A” is the CO2 gas cylinder connected to

the CO₂gas pipe connected to port A, and “gas cylinder B” is the CO2 gas cylinder connected to the CO₂

gas pipe connected to port B.)

㩷

3. Open the valves of both gas cylinders.

㩷

4. The current CO₂gas supply line is displayed on the Top Display.

(“A” and “B” will be displayed only when the MCO-21GC is installed. Nothing will be displayed if

MCO-21GC is not installed.)

㩷

Current CO₂gas supply line

㩷

T e m p

3 7 .

C O 2

5 . 0 %

㩷

ꢁꢁ㧡ꢀ㧜ꢁ

㧟㧣ꢀ㧜ꢁ

S t a t u s

O K

5. The MCO-21GC detects that there is no more CO₂gas in a cylinder when the CO₂density in the

chamber does not increase for a while even after opening the CO₂gas valve in the Incubator. It switches

the gas supply line when it detects that there is no more gas. At that time, the following indications of

switching the gas cylinder are provided.㩷

㩷

Message display field

Display of gas supply

The character for gas supply line

that is empty will flash

When CO₂gas supply line switches

automatically (Only when MCO-21GC

is installed)

Err01: CO₂Gas Empty is

㩷

The gas supply line that is

currently being used is displayed

in reverse video.

displayed.

䃂To silence the buzzer, press the BUZZER Key. The alarm condition is cleared and normal operation

continues.

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6. When gas cylinder A is switched to B, remove gas cylinder A and replace it with new one.

Note: Be careful when handling the empty CO₂gas cylinder. Some gas may still be left in the cylinder.

ƔWhen gas cylinder B becomes empty, the line is switched to gas cylinder A again.

The MCO-21GC detects that there is no more CO₂gas in a cylinder when the CO₂density in the chamber

does not increase for a while even after opening the CO₂gas valve in the Incubator. The supply line may

also be switched for other reasons even though there is gas left in the cylinder: blocking or restricting of

gas tube, reduction of CO₂gas pressure, or improper opening of CO₂gas cylinder. Always check the gas

quantity in the cylinder before disconnecting it.

㩷

Ɣ Use the following procedure to manual switch the line between CO₂cylinders A and B (only when

MCO-21GC is installed). This procedure shows switching the line from㩷 CO₂cylinder B to A as an

example.㩷

㩷

1. From the Top Display, press the Left Cursor Key for 5 seconds.

㩷

T e m p

3 7 .

C O 2

5 . 0 %

㩷

ꢁꢁ㧡ꢀ㧜ꢁ

㧟㧣ꢀ㧜ꢁ

S t a t u s

O K

2. The CO₂gas supply line will be switched from cylinder B to A㩷

㩷

T e m p

3 7 .

C O 2

5 . 0 %

㩷

ꢁꢁ㧡ꢀ㧜ꢁ

㧟㧣ꢀ㧜ꢁ

S t a t u s

O K

(When the line needs to be switched from cylinder A to B, press the Right Cursor Key for 5 seconds.) 㩷

㩷

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AUTOMATIC CO₂DENSITY CALIBRATION

㩷

To calibrate the CO₂density, an Automatic CO₂Standard Gas Calibration Kit (MCO-SG) is available. By

connecting a CO₂standard gas cylinder that has a density that is the same as the set CO₂density (usually

5% CO₂), the CO₂sensor can be calibrated to maintain the accuracy of the CO₂density display as

necessary.

㩷

Ɣ Setting connected standard CO₂gas density

1. Press the MENU Key from the Top Display to display the menu, select Tools, and press the ENTER

Key.

㩷

T e m p

3 7 .

C O 2

5 . 0 %

㩷

M E N U

S e t

ꢁꢁ㧡ꢀ㧜ꢁ

㧟㧣ꢀ㧜ꢁ

L o g

T o o l

S t a t u s

O K

2. Press the Down Cursor Key from the Select Tools (1/2) Display to display the Select Tools (2/2) Display,

and then select Standard Gas Setting, press the MENU Key to display the menu, select OK, and press

the ENTER Key.

㩷

S e l e c t

T o o l

C a l

n g

(

1 / 2 )

㩷

T e m p / C O 2

b r a t o n

㩷

M E N U

O K

A l a r m

S e t

L C D / D A Q

S e t

n g

S v c

U V

S e t

L o c k

T i m e

C a n c e l

K e y

D a t

P W

S e t

n g

S e l e c t

T o o l

(

2 / 2 )

S t a n d a r d

G a s

S e t

C a l

n g

M E N U

O K

b r a t

o n

S v c

C a n c e l

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㩷

3. Input the CO₂density of standard gas to be used, press the MENU Key to display the menu, select OK,

and press the ENTER Key.

㩷

S t a n d a r d

G a s

S e t

n g

㩷

M E N U

O K

C O 2

S T D

G a s

D e n s i

5 .

C a n c e l

Ɣ Calibration method for CO₂gas density

1. Select Standard Gas Calibration from the Select Tools (2/2) Display, press the MENU Key to display

the menu, select OK, and press the ENTER Key.

㩷

S e l e c t

T o o l

(

2 / 2 )

㩷

S t a n d a r d

G a s

S e t

C a l

n g

㩷

b r a t

o n

M E N U

O K

S v c

C a n c e l

2. The system check will start.

㩷

S t a n d a r d

G a s

C a l

b r a t

o n

S t e p 㧦

㩷

S t

G a s

C a l

S y s t e m

C h e c k

࡮

C O 2 㧦 0 . 0 %

S T G 㧦 5 . 0 %

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㩷

3. If the system is normal, the following display will appear. Select OK and press the ENTER Key to start

calibrating the CO₂density. After calibration, return to normal cultivating operation.

㩷

S t a n d a r d

G a s

C a l

b r a t i o n

㩷

M E N U

O K

R e a d y

S t a r t

C a n c e l

C O 2 㧦 0 . 0 %

S T G 㧦 5 . 0 %

S t a n d a r d

G a s

C a l

b r a t

o n

S t e p 㧦

S t

G a s

C a l

b r a t

n g

࡮

C O 2 㧦 4 . 8 %

S T G 㧦 5 . 0 %

T e m p

3 7 .

C O 2

5 . 0 %

ꢁꢁ㧡ꢀ㧜ꢁ

㧟㧣ꢀ㧜ꢁ

S t a t u s

O K

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STACKING INCUBATORS

Use the following procedure to stack Incubators. This work is potentially dangerous, so contact a Sanyo

representative or agent.

1.㩷 Take out the stacking brackets (A and B), the protective stickers, and the four screws from the

accessory bag.

CAUTION

When stacking two Incubators, use two stacking brackets (A and B) for each one. Only one pair of

stacking brackets is provided per Incubator, so take out the brackets (A and B) from both Incubators.

2. Unplug both of the Incubators that are to be stacked.

3. Attach the protective stickers to the four corners on the top surface of the Incubator that is to be on the

bottom. (See Fig. A.)

4. Remove the caps at the two places in the front of the top surface of the Incubator that is to be on the

bottom, and secure stacking bracket A with the screws that are provided.

5. Remove the power supply panel (4 screws, wiring connectors in 3 places, ground wire, and gas pipe)

from the Incubator that is to be on the top.

6. Place the upper Incubator on top of the lower one, being careful not to pinch your fingers.

7. Make the lower Incubator level by adjusting the legs, and then level the upper Incubator in the same

way.

8. Secure stacking bracket A to the upper and lower Incubators with the accessory screws.

9. Remove from the back of the lower Incubator the two stabilizing brackets (4 screws) and remove from

the upper Incubator the 2 screws on the lower rear panel.

10. Use the 6 screws that were removed in step 9 to secure stacking bracket B to the rear panels of the

upper and lower Incubators.

11. Reconnect the power supply panel (4 screws, wiring connectors in 3 places, ground wire, and gas

pipe) to the upper Incubator.

12. Use the stabilizing bracket on the back of the upper Incubator to prevent the Incubators from falling

over.

CAUTION

x Select a floor that is strong enough to support the stacked Incubators.

x Never stack three or more Incubators. Doing so is dangerous.

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Front panel

Stacking plate B

Front

Stacking plate A

Protective sticker

Hook

Fig.A

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SPECIFICATIONS

Name

CO₂Incubator

Models

MCO-19AIC

MCO-19AIC(UV)

External dimensions

Internal dimensions

Interior volume

Exterior

620 x 710 x 900 mm (W x D x H)

490 x 523x 665 mm (W x D x H)

170 L

Painted steel

Interior

Stainless steel containing copper

Painted steel

Outer door

Inner door

Tempered glass

Trays

4 trays made of stainless steel containing copper

450 x 450 x 12 mm (W x D x H), Maximum load: 7 kg/tray

Inner diameter: 30 mm, On the back side

Access port

Insulation

Rigid polyurethane foamed-in place

DHA system (heater jacket + air jacket system)

314 W

Heating system

Heater

Humidifying system

Temperature controller

Temperature display

CO₂controller

CO₂density display

Air circulation

Air filter

Natural evaporation with humidifying pan

PID control system

Digital display

PID control system

Digital display

Fan assisted

0.3 Pm, Efficiency: 99.97% or higher

-----

4W X 1 (ozone-free emission)

UV lamp

Optical

Water level sensor

Alarms

Automatic set temperature alarm, Automatic set CO2 density alarm,

Upper limit temperature alarm, Door alarm, Low humidifying water alarm,

UV lamp failure alarm (for UV equipped model only)

Remote alarm contacts

CO₂inlet connection

CO₂inlet pressure

Accessories

Allowable contact capacity: 30 VDC, 2 A

4 to 6 mm diameter tube

0.03 MPaG (0.3 kgf/cm²G, 4.3 psiG)

4 trays, 4 sets of tray supports, 1 gas tube, 1 humidifying pan,

stacking plates A and B, 4 protective stickers, 2 tube bands

93 kg

Weight

UV Lamp Add-on Kit (MCO-19UVS)

Standard equipment

H₂O₂Generator (MCO-HP), H₂O₂Decontamination Kit (MCO-HL),

H₂O₂Decontamination Reagent (MCO-H2O2),

Automatic CO₂Standard Gas Calibration Kit (MCO-SG),

Gas Pressure Regulator (MCO-100L),

Extra Trays (MCO-47ST, includes 2 tray supports),

Optional accessories

Half Trays (MCO-25ST), Automatic CO₂Cylinder Changeover System (MCO-21GC),

Roller Base (MCO-18RB), Communications Terminal (MTR-480/MTR-L03)

Note: Designs and specifications are subject to change without notice.

The ballast is not user replaceable. Please contact qualified service personnel.

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PERFORMANCE

Ambient temperature+5 to 50^oC (ambient temperature: 5 to 35^oC)

0.25^oC* (ambient temperature: 25^oC, setting: 37^oC, CO₂: 5%, no load)

0.1^oC (ambient temperature: 25^oC, setting: 37^oC, CO₂: 5%, no load)

Temperature control range

Temperature distribution

Temperature variation

CO₂control range

0 to 20%

0.15% (ambient temperature: 25^oC, setting: 37^oC, CO₂: 5%, no load)

CO₂variation

95% 5%

1,120 kJ/h

Chamber humidity

Maximum heat emission

Temperature: 5 to 35^oC, Humidity: 80% max.

(The designed performance may not be obtained if

the ambient temperature is equal or less than 15^oC)

Applicable environment

condition

30 dB (A scale)

Noise level

350 W

110 to 120 V, 60 Hz

2.8 A

310 W

220 to 240 V, 50 Hz

1.4 A

Maximum power consumption

Total maximum

current

Note: 㪫㪿㪼㩷㫌㫅㫀㫋㩷㫎㫀㫋㪿㩷㪚㪜㩷㫄㪸㫉㫂㩷㪺㫆㫄㫇㫃㫀㪼㫊㩷㫎㫀㫋㪿㩷㪜㪚㩷㪻㫀㫉㪼㪺㫋㫀㫍㪼㫊㪅㩷

* Based on our measuring method.

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CAUTION

Please fill in this form before servicing.

Hand over this form to the service engineer to keep for his and your safety.

Safety check sheet

1. Unit contents :

Yes

Risk of infection:

Risk of toxicity:

Risk from radioactive sources:

(List all potentially hazardous materials that have been stored in this unit.)

Notes :

2. Contamination of the unit

Unit interior

Yes

No contamination

Decontaminated

Contaminated

Others:

3. Instructions for safe repair/maintenance of the unit

a) The unit is safe to work on

Yes

b) There is some danger (see below)

Yes

Procedure to be adhered to in order to reduce safety risk indicated in b) below.

Date :

Signature :

Address, Division :

Telephone :

Product name :

CO₂incubator

Model :

MCO-19AIC(UV)

MCO-19AIC

Serial number :

Date of Installation :

Please decontaminate the unit yourself before calling the service engineer.

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SANYO Electric Co., Ltd

Printed in Japan

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