Novocastra™ Liquid
Mouse Monoclonal Antibody
CD33
Product Code: NCL-L-CD33
FOR RESEARCH USE ONLY. NOT FOR USE IN DIAGNOSTIC PROCEDURES.
Intended Use
Specificity
Clone
Human CD33 antigen, both isoforms; CD33m (31 kD) and CD33M (67 kD).
PWS44
IgG2b
Ig Class
Prokaryotic recombinant protein corresponding to a region of the C2 domain of the human CD33
molecule.
Antigen Used for
Immunizations
Mouse myeloma (p3-NS1-Ag4-1).
Hybridoma Partner
Liquid tissue culture supernatant containing sodium azide.
Volume as indicated on vial label.
Preparation
Not evaluated.
Yes
Effective on Frozen Tissue
Effective on Paraffin Wax
Embedded Tissue
Immunohistochemistry on paraffin sections.
Recommendations on Use
Heat Induced Epitope Retrieval (HIER): Please follow the instructions for use in Novocastra
Epitope Retrieval Solution pH 9.
Suggested dilution: 1:100 for 30 minutes at 25 °C. This is provided as a guide and users should
determine their own optimal working dilutions.
Visualization: Please follow the instructions for use in the Novolink™ Polymer Detection Systems.
For further product information or support, contact your local distributor or regional office of Leica
The performance of this antibody should be validated when utilized with other manual staining
systems or automated platforms.
Western Blotting: Typical working dilution 1:100–1:500.
Immunohistochemistry: Bone marrow or granulocytic sarcoma.
Western Blotting: U937 cell line.
Positive Controls
Cytoplasmic and Membrane.
Staining Pattern
Store at 2–8 °C. Do not freeze. Return to 2–8 °C immediately after use. Do not use after
expiration date indicated on the vial label. Storage conditions other than those specified above
must be verified by the user.
Storage and Stability
This reagent has been prepared from the supernatant of cell culture. As it is a biological product,
reasonable care should be taken when handling it.
Warnings and Precautions
This reagent contains sodium azide. A Material Safety Data Sheet is available upon request or
Leica Biosystems Newcastle Ltd
Balliol Business Park West
Benton Lane
Newcastle Upon Tyne NE12 8EW
United Kingdom
(
+44 191 215 4242
CD33-L 13/05/2014
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© Leica Biosystems Newcastle Ltd. NCL-L Rev D
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