Leica MZ16 F
User manual
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Operating controls
10
9
8
7
6
4
5
1
2
3
Fig. 1 Leica MZ16 F with trinocular video/phototube and Leica digital camera
7 Trinocular video/phototube
1 Zoom magnification changer
8 Adjustable tubes for adjusting the
interpupillary distance from 52mm–76mm
9 Knurled rings for adjusting the diopter settings
from +5 to –5
2 Coarse/fine focusing drive
3 Double iris diaphragm
4 Rapid filter changer for four selectable filter sets
5 Filter holder for an individually selectable filter
10 Adjustable and attachable eyecups
6 Switch for activating/deactivating the
UV shutter
3
Leica MZ16 F – User manual
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Contents
Page
Page
1. Safety concept . . . . . . . . . . . . . . . . . . . . . . . . . .5 4. Operation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
1.1 The user manual . . . . . . . . . . . . . . . . . . . . . . . . .5 4.1 Description . . . . . . . . . . . . . . . . . . . . . . . . . . . . 18
1.1.1 Diagrams . . . . . . . . . . . . . . . . . . . . . . . . . . .5
1.1.2 Symbols used . . . . . . . . . . . . . . . . . . . . . .6
1.2 Safety instructions . . . . . . . . . . . . . . . . . . . . . . .6
4.1.1 Fluorescence technology . . . . . . . . . . .18
4.1.2 Functional principle . . . . . . . . . . . . . . . .18
1.3 Light source . . . . . . . . . . . . . . . . . . . . . . . . . . . . .9 5. Functions . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .18
5.1 Stereomicroscope settings
2. Overview of the instrument . . . . . . . . . . . . . .10
and adjustments . . . . . . . . . . . . . . . . . . . . . . . .18
5.1.1 Observation without fluorescence . . . .19
5.1.2 Double iris diaphragm . . . . . . . . . . . . . .19
3. Assembly . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .12
3.1 Assembly sequence . . . . . . . . . . . . . . . . . . . .12 5.2 Commissioning the fluorescence system . .20
3.1.1 Expansion diagram . . . . . . . . . . . . . . . . .13 5.3 Rapid filter changer . . . . . . . . . . . . . . . . . . . . .20
3.2 Assembly of stereomicroscope with stand . .14 6. Work flow . . . . . . . . . . . . . . . . . . . . . . . . . . . . .21
3.3 Additional components . . . . . . . . . . . . . . . . . .15
3.3.1 Video/phototube . . . . . . . . . . . . . . . . . . .15 7. Appendix . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
7.1 Filter sets . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .22
3.4 Light source . . . . . . . . . . . . . . . . . . . . . . . . . . . .15 7.2 Technical data . . . . . . . . . . . . . . . . . . . . . . . . .23
3.4.1 Lamp housing 106 z . . . . . . . . . . . . . . . . .15 7.3 Optical data . . . . . . . . . . . . . . . . . . . . . . . . . . .27
3.4.2 Supply units . . . . . . . . . . . . . . . . . . . . . . .15 7.4 Dimensions . . . . . . . . . . . . . . . . . . . . . . . . . . . .28
3.5 UV protection screen . . . . . . . . . . . . . . . . . . . .16
3.6 Equipping the filter changer . . . . . . . . . . . . . .16
3.6.1 Filter sets for fluorescence . . . . . . . . . .16
3.6.2 Simple filter holders . . . . . . . . . . . . . . . .17
3.6.3 Inserting the filter . . . . . . . . . . . . . . . . . .17
3.6.4 Slot for filter slide . . . . . . . . . . . . . . . . . .17
4
Leica MZ16 F – User manual
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1. Safety concept
1.1 The user manual
Before installing, operating or using the
instruments, read the user manuals listed above.
In particular, please follow all safety instructions.
To maintain the unit in its original condition and to
ensure safe operation, the user must follow the
instructions and warnings contained in these user
manuals.
Included with your Leica MZ16 F fluorescence
stereomicroscope is an interactive CD-ROM with
all pertinent user instructions in German, English,
French, Spanish, Italian, Portuguese, Dutch, Dan-
ish, Swedish, Finnish and Greek. Keep it in a safe
place, and readily accessible to the user. User
manuals and updates are also available for you to
download and print from our web site at
1.1.1 Diagrams
(1.2) Numbers in parentheses within the descrip-
tions refer to diagrams and the items in these
diagrams. Example (1.2):
The Leica MZ16 F is the manual fluorescence
stereomicroscope of the Leica M stereomicro-
scope series. This operating manual describes
the special functions of the Leica MZ16 F and
important instructions for its operational safety,
maintenance, and accessories. Like all instru-
ments of the Leica M stereomicroscope series,
the Leica MZ16 F consists of modules, which
allow an almost unlimited variety of custom
equipment configurations. The identical modules,
such as stands, binocular tubes, accessories, etc.
are described in User Manual M2-105-0 for
Leica M stereomicroscopes, which also contains
additional safety instructions pertaining to the
stereomicroscope, accessories and electrical
accessories, as well as care instructions.
Figure 1 is located on page 3, and item 2 is the
focusing drive.
Special manuals are provided for a number of
accessories:
– User Manual M2-216-1 for lamp housing with
high-pressure mercury burner.
– User Manual for ebq 100 isolated
supply unit for 100W high-pressure
mercury burners.
– User Manual M2-267-1
for the motorized focus system.
– User Manual M2-166-2 for
TM
FluoCombi III
5
Leica MZ16 F – Safety concept
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1.1.2 Symbols used
1.2 Safety instructions
Description
Warning of a danger
The Leica MZ16 F is a manual fluorescence stere-
omicroscope with 16:1 zoom for fluorescence
applications. The optics system consists of
two observation beam paths, a patented Triple-
This symbol indicates especially important infor-
mation that, if not observed,
– can cause hazards to personnel,
– can lead to functional disturbances and dam-
aged instruments.
®
Beam third beam path specifically for fluores-
®
cence illumination and the patented FLUOIII filter
system. The entire imaging system, including the
®
16:1 zoom, objective and ErgoTube is apochro-
Warning of hazardous electrical voltage
This symbol indicates especially important infor-
mation that, if not observed,
– can cause hazards to personnel,
– can lead to functional disturbances and
damaged instruments.
matically corrected.
The Leica MZ16 F consists of these modules:
– Stereomicroscope
– Stand, binocular tube, objective, eyepieces and
(if applicable) accessories for photography,
etc.
– Power supply
– Filter sets with barrier and excitation filters on
one filter holder
– Three simple filter holders for transmitted light
observation and protection from UV light
– Filter holder for individual filters
– UV protection screen
Danger due to hot surface
This symbol warns against touching hot surfaces,
e.g. those of light bulbs.
Important information
This symbol indicates additional information or
explanations that intend to provide clarity.
– 106 z lamp housing with 50W or 100W high-
pressure mercury burner with stray-light
protection
Action
– Supply unit
̈ This symbol refers to actions described in the
text that are to be carried out.
Additional notes
•
This symbol indicates additional notes and
explanations provided in the text.
6
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Permitted uses
Place of use
With the Leica MZ16 F fluorescence stereomicro-
scope, entire fluorescent specimens can be
examined three-dimensionally and unprepared,
manipulated, sorted and documented. The sepa-
rate beam path for the fluorescence illumination
in the Leica MZ16 F as well as the very bright
high-pressure mercury burner and specially
selected filter sets enable you to distinguish the
finest structures and enhance the quantity of
information for incident light fluorescence. Spe-
cial filter holders also enable observation without
fluorescence in transmitted light or incident light.
– The Leica MZ16 F may only be operated in a
closed, dust-free room, which is free of oil
vapors and other chemical vapors, as well as
extreme humidity, and within a temperature
range of +10 to +40.
– Electrical components must be assembled at
least 10 cm away from the wall and from flam-
mable substances.
– Large temperature fluctuations, direct sunlight
and vibrations should be avoided. These condi-
tions can distort measurements and micro-
graphic images.
– Stereomicroscopes in warm and warm-damp
climatic zones require special care in order to
prevent the build up of fungus.
Non-intended use
If the Leica MZ16 F, its components and acces-
sories are used other than as described in the
user manual, injury or property damage may
occur.
Responsibilities of the person(s) in charge of
instrument
Never:
Ensure that
– install other plugs or cables.
– change, rebuild or take apart parts, if not
specifically instructed to in this manual.
– allow unauthorized persons to open parts.
– use the MZ16 F for examining, or operating on
the human eye.
– the Leica MZ16 F and accessories are oper-
ated, maintained and repaired only by autho-
rized and trained personnel.
– the operating personnel has read, understands,
and is observing these instructions as well as
those listed on page 5, and, in particular, the
safety instructions.
The devices and accessories described in this
User manual have been tested for safety and
potential hazards. The responsible Leica affiliate
or the main plant in Wetzlar must be consulted
whenever the device is altered, modified or used
in conjunction with non-Leica components that
are outside of the scope of this manual.
Unauthorized alterations to the device or noncom-
pliant use shall void all rights to any warranty
claims!
Transport
– Use the original packaging for shipping or
transporting the stereomicroscope and its
accessory components.
– In order to prevent damage from vibrations,
disassemble all moving parts that (according to
the user manual) can be assembled by the cus-
tomer and pack them separately. These include
the objective, binocular tube, eyepieces, lamp
housing, burner in the 106 z lamp housing, etc.
Leica MZ16 F – Safety concept
7
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Repairs and servicing
Health risks
Workplaces with stereomicroscopes both
improve and make visual tasks easier, but they
also place high demands on the human visual
apparatus and the muscular "holding apparatus"
of the user. Depending on the duration of uninter-
rupted work, asthenopia and musculoskeletal
problems may occur. For this reason, appropriate
measures for reduction of the workload must be
taken:
– Only Leica Microsystems-trained service tech-
nicians, or technical specialists assigned by
the person in charge of the instrument, are
permitted to carry out repairs.
– Only original Leica Microsystems spare parts
may be used.
– Disconnect the power cable before opening
voltage-carrying parts such as the power
supply or the supply unit.
– optimal arrangement of workplace, work
assignments and work flow (changing tasks
frequently).
– thorough training of the personnel, giving
consideration to ergonomic and organization
aspects.
Touching the live circuit can cause injury.
Installation in third-party products
When installing Leica products into third-party
products, note the following:
The manufacturer of the complete system or its
dealer is responsible for following all applicable
safety instructions, laws and guidelines.
The ergonomic design and construction of
Leica M series stereomicroscopes are intended
to reduce the exertion of the user to a minimum.
Disposal
– The product must be disposed of in accordance
with locally applicable laws and regulations.
– Dispose of used high-pressure mercury burn-
ers as hazardous waste.
Direct contact with eyepieces is a potential
transmission method for bacterial and viral infec-
tions of the eye.
The risk can be kept to a minimum by using per-
sonal eyepieces for each individual or detachable
eyecups.
Legal requirements
Adhere to general and local regulations relating to
accident prevention and environmental protec-
tion.
Conformity with European Community directive
The Leica MZ16 F and its accessories are con-
structed in accordance with the latest technolo-
gies and are provided with a statement of confor-
mity with EC requirements.
8
Leica MZ16 F – Safety concept
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1.3 Light source: safety instructions
Lamp housing
Precautions by the manufacturer
The Leica MZ16 F may be used only together with
the Leica 106 z lamp housing for the 100W or 50W
mercury burner, supply unit and stray-light protec-
tion.
– UV protection screen in front of the specimen
plane prevents the user from looking directly
into the UV rays.
Simple filter holders (that have only two open-
ings for the observation beam paths) in the avail-
able positions of the rapid filter changer prevent
direct UV radiation from reaching the eyes.
– UV shutter (which can be activated/deacti-
vated) prevents direct UV radiation from reach-
ing the eyes.
–
Leica Microsystems accepts no liability for
damages resulting from the use of lamp housings
from other manufacturers.
– Never open the lamp housing while the lamp is
switched on. Unplug the supply unit from the
power supply:
– UV filters in the observation beam path protect
the eyes.
– The stray-light protection on the lamp housing
prevents irradiation of the hands.
Risk of explosion, UV exposure, blinding!
– Before opening the lamp housing, allow it to
cool off for at least 15 minutes.
Warning
Danger of explosion, danger of burns.
UV radiation can harm the eyes.
– Never cover the air duct on the lamp housing.
Danger of fire!
Therefore:
– Never look into the light spot on the specimen
plane without a UV protection screen.
– Always fill all of the filter positions with filter
holders.
– Use the stray-light protection.
Danger to eyes from UV radiation.
– Fill empty filter positions with the simple filter
holders (which have only two openings for the
observation beam paths).
– Do not select a white, strongly reflective
background for the stage.
– Never open the lamp housing while the lamp is
switched on.
Risk of explosion, UV exposure, blinding!
Supply unit
Always unplug the supply unit from the power
supply:
– when installing and disassembling the lamp
housing
– before opening the lamp housing
– when replacing the high-pressure mercury
burner and other parts, such as the heat-
absorbing filter or the collector
– during maintenance work on the supply unit.
Leica MZ16 F – Safety concept
9
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2. Overview of the instrument
6
5
4
12
2
3
9
10
13
11
1
®
Fig. 2 Left side of the Leica MZ16 F with ErgoTube from the user’s perspective
1 Stand (transmitted or incident light stand with
corresponding illumination). Pictured here is
the HL RC™ transmitted-light base for best
relief contrast.
2 Focusing drive: (pictured) manual coarse/fine
drive with column (see User Manual M1-105-0)
or motorized focus system (see User Manual
M2-267-1)
4 The Leica MZ16 F optics carrier with two
®
observation beam paths and a TripleBeam
®
third beam path, motorized FLUOIII filter sys-
tem and adapter for 106 z lamp housing
®
5 ErgoTube with variable viewing angle
of 10° to 50° (pictured) or trinocular tube
(in Figure 3.5)
6 Wide-field eyepieces for persons wearing
glasses
3 Microscope carrier or FluoCombi™ (changer
for one planapochromatic and one HR micro-
scope objective)
10
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6
5
12
8
7
4
3
10
9
2
13
11
1
Fig. 3 Right side of the Leica MZ16 F from the user’s perspective with trinocular tube, video objective
and Leica DFC300 FX digital camera for fluorescence
7 Filter sets: the scope of delivery includes three
simple filter holders (which have only two
openings for the observation beam paths) for
empty filter positions and transmitted or inci-
dent light observation without fluorescence.
Additionally, various filter sets of your choice
for fluorescence (excitation and barrier filter on
one filter holder) are available.
8 Filter holder for an individually selectable filter,
such as a gray filter
9 Interchangeable objective planachromatic or
planapochromatic
10 UV protection screen with arm
11 Gliding stage (optional)
12 Leica 106 z lamp housing for 50W or 100W mer-
cury burner with stray-light protection (not pic-
tured)
13 Supply unit for lamp housing 106 z
Leica MZ16 F – Overview of the instrument
11
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3. Assembly
Basic components such as the stand, column,
microscope carrier, binocular tubes and optical
accessories are, thanks to their modular design,
identical for all Leica M series stereomicro-
scopes. Therefore, for assembly instructions,
please refer to the User Manual for Leica M
stereomicroscopes, M2-105-0. The assembly of
the UV aperture protection is described in this
User Manual. The lamp housing, supply unit and
adjustment of the high-pressure mercury burner
are described in User Manual M2-216-1.
3.1 Assembly sequence
The microscope components are best assembled
in this order:
– Stand: base and column with focusing drive
(manual or motorized) and focusing stop (for
motorized focus and FluoCombi III™)
– Microscope carrier or FluoCombi III™
– Leica MZ16 F optics carrier
– Binocular or trinocular tube
– Eyepieces and eyecups
– Stage plate or special stage (gliding stage,
heating stage)
– Objective
– UV protection screen with arm
– Lamps: high-pressure mercury burner in lamp
housing; for the HL RC™ transmitted-light
stand, fiber-optic light guide with light source
– Supply unit for lamp housing
– Electrical connections (for motorized focus and
illuminators)
– Equipment of filter changer
– Equipment of the filter insert with an individual
filter, where applicable
Either the integrated clamping screws or an Allen
key is necessary for assembly; these are included
in delivery.
12
Leica MZ16 F – Assembly
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10 447 160
10 445 301
10 445 302
10 445 303
3.1.1 Expansion diagram
106z
11 504 066
11 504 069
10 446 229
10 445 822
HD-50
10 446 197
HD F 10 446 308
HD V 10 446 309
10 446 271
ICA 10 446 237
10 446 238
Filter Sets
10 447 220
10 447 221
10 447 217
10 447 222
10 447 218
10 447 223
10 447 219
10 447 224
10 447 225
10 447 226
10 447 227
10 447 215
10 447 216
10 446 260
RS 232
10 447 080
10 446 182
MZ16 F
10 447 064
MZ16 FA
10 447 063
10 447 157
10 447 051
10 447 050
10 446 157
10 445 819
10 447 101
10 447 075
Plan/Planapo
Motor focus
10 446 176 / 10 447 041
Leica MATS
10 447 165
10 446 227
TM
FluoCombi III
10 447 324
10 446 301
Leica MATS
10 447 164
10 447 106 / 10 447 185
10 445 631
10 445 363
10 445 387
10 445 367 HL
TM
10 446 359 HL-RC
Leica MZ16 F – Assembly
13
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Microscope carrier ➔ focusing drive
̈ Fasten the microscope carrier (4.3) to the
Leica MZ16 F according to User Manual
M2-105-0 on the focusing drive (4.2).
Tool: Allen key.
3.2 Assembly of stereomicroscope with
stand
Do not yet connect the peripherals to the
power supply!
Or, if available:
Focusing drive ➔ stand base
̈ Fasten the FluoCombi III™ to the focusing drive
(4.2) as described in User Manual M2-166-2.
Tool: Allen key.
̈ Fasten the manual or motorized focusing drive
(4.2) to the column according to User Manual
M2-105-0 (manual) or M2-267-1 (motorized) on
the base (4.1).
Optics carrier ➔ microscope carrier
Tool: Allen key.
̈ For motorized focus and FluoCombi III™, fasten
the focusing stop to the stand column under the
focusing drive using a clamping screw.
The Leica MZ16 F optics carrier and the
®
FLUOIII filter system make up a single unit,
which is factory-adjusted. They may not be taken
apart.
When using the motorized focus, be certain
to read the corresponding User Manual M2 -267-1,
in particular the safety instructions.
̈ Insert the Leica MZ16 F optics carrier (4.4)
according to User Manual M2-105-0 into the
microscope carrier (4.3) or into the FluoCombi
III™ and fasten it using a clamping screw.
6
5
8
2
4
3
1 Base
2 Focusing drive
3 Microscope carrier
4 Optics carrier
5 Video/photo tube
6 Eyepiece
9
7
1
7 Objective
8 Lamp housing 106 z
9 Supply unit
Fig. 4 Right side of the Leica MZ16 F
14
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3.3 Additional components
3.4 Light source
̈ Fasten the other components and other individ- The Leica 106 z lamp housing is the light source
ual accessories according to User Manual
M2-105-0:
for the Leica MZ16 FA and is used with 100W or
50W mercury lamps, corresponding supply units
and stray-light protection.
– Insert the binocular tube (2.5) or the video/
phototube into the dovetail ring on the
®
FLUOIII filter system and fasten it using a
clamping screw.
Please read
– Insert the eyepieces (4.6) in the tubes and
fasten them using the clamping screws.
– If necessary, place soft eyecups for prevent-
ing eye infections over the built-in eyecups
(see User Manual M2-105-0).
– Screw the objective (4.7) into the bottom of
the optics carrier (4.4) counterclockwise.
– the separate User Manual M2-216-1 for the
Leica lamp housing
– the user manual and safety instructions for the
ebq 100 supply unit
– the user manual and safety instructions pro-
vided by the lamp manufacturer, and, in partic-
ular, the section on how to proceed if a lamp
breaks and releases mercury.
3.3.1 Video/photo tube
Do not switch on the supply unit until you
have read and familiarized yourself with the
functions of the Leica MZ16 F, provided in the
"Operation" chapter.
We recommend using the trinocular video/photo-
tube (4.5) or the HD-V video/phototube with 100%
light in the video/photo output, in order to achieve
shorter exposure times at low fluorescence levels.
̈ Insert the video/photo tube (4.5) into the ring
After it is switched on, the burner must be
adjusted immediately.
®
connector on the FLUOIII filter system accord-
ing to User Manual M2-105-0 and fasten it
using the clamping screw.
̈ Fasten a binocular tube to the HD-V
3.4.1 Lamp housing 106 z
video/photo tube using a clamping screw.
Fasten the 106 z lamp housing (4.8) to the adapter
part as described in User Manual M2-216-1.
3.4.2 Supply units
The supply unit for 50W mercury lamps is
described in User Manual M2-216-1.
The 100W mercury lamps are powered by the ebq
100 (4.9) supply unit.
Please read the separate instruction manual
provided by the manufacturer.
Leica MZ16 F – Assembly
15
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3.5 UV protection screen ➔ microscope 3.6 Equipping the filter changer
carrier
®
The patented FLUOIII filter system consists
̈ Fasten the UV protection screen (5.1) using a
hexagon-head screw (5.3) to the left or right
side of the microscope carrier.
of a filter changer for barrier and excitation filters,
a UV shutter that can be activated/deactivated,
and a filter insert for a filter holder with your
choice of filter.
Tool: Allen key.
The filter changer can hold a total of four filter
sets. The filter sets for fluorescence are labeled
(see page 22). All filter sets are equipped with a
transponder for automatic filter detection and can
also be used with the motorized Leica MZ16 FA.
Always position the UV protection screen
so that the operator can never look directly at the
light spot (see page 9):
̈ Set the UV protection screen (5.1) laterally
using the arm (5.2).
̈ Loosen the hexagon-head screw (5.3).
̈ Move the UV protection screen up or down
using the arm.
̈ Tighten the hexagon-head screw (5.3).
The scope of delivery of the Leica MZ16 F includes
three filter holders without fluorescence for empty
filter positions and transmitted or incident light
observation without fluorescence. These filter
holders have two empty openings for the observa-
tion beam paths. The third opening to the illumina-
tion beam path is closed.
If you are using fewer than four filter hold-
ers with fluorescence filters, always insert the
included simple filter holders with closed illumi-
nation beam path into the available positions.
Otherwise, there is a risk of danger to the eyes
from direct UV radiation from the third beam path.
Also, they protect the system from dust.
3
3.6.1 Filter sets for fluorescence
2
The filter sets for fluorescence contain, on one
filter holder, two barrier filters to the visual beam
paths and one excitation filter to the illumination
beam path. The fluorescence filters have a
transponder with the specific filter data for use
with the motorized Leica MZ16 FA. The filter sets
are labeled (see page 22).
1
Fig. 5 UV protection screen
1 UV protection screen
2 Arm
3 Hexagon-head screw
16
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3.6.2 Simple filter holders
3.6.3 Inserting the filter
Three simple filter holders are included in the
scope of delivery of the Leica MZ16 F. These filter
When inserting the filter sets, avoid touch-
holders have two empty openings for the observa- ing the filters if at all possible in order to avoid
tion beam paths. The third opening, to the illumi-
nation beam path, is closed. Use these filter
holders
fingerprints. If you touch them, clean the filters
using a soft, lint-free cloth and pure alcohol.
̈ Insert the filter holder (6.1) into the filter
changer such that the label (e.g. GFP1) is
upright and legible on the right side of the
slide slot.
– if you want to work briefly (no more than
15 seconds) without fluorescence illumination.
•
Ensure that the contour of the filter holder
matches the contour of the microscope.
To avoid overheating the filter holder, do
not use the filter holder to block the mercury light
for more than 15 seconds.
̈ Rotate the filter changer by hand until it latches
into place.
•
If the filter holder has not been inserted
correctly, the filter changer does not rotate.
– If you want to block the mercury light for longer
than that, activate the UV shutter (see page 19). Insert a total of four filter holders.
– if you want to change to transmitted light or
incident light observation without fluorescence.
– at all times in empty filter changer positions, in
3.6.4 Slot for filter slide
order to protect yourself from UV radiation and
protect the system from dust.
There is a slot (6.2) in the filter changer system for
an individually selectable filter, for example a gray
filter.
If you are using fewer than four filter hold-
ers with fluorescence filters, always insert the
included simple filter holders with closed illumi-
nation beam path into the available positions.
Otherwise, there is a risk of danger to the eyes
from direct UV radiation from the third beam path.
2
1
Fig. 6 Filter changer, equipped with filter holders
1 Filter changer with filter sets
2 Filter slide for filter holder with individual filters
Leica MZ16 F – Assembly
17
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4. Operation
5. Functions
4.1 Description
5.1 Stereomicroscope settings and
adjustments
4.1.1 Fluorescence technology
Fluorescent substances light up (fluoresce) when
irradiated with short-wave excitation light. Fluo-
rescence technology makes use of this property
by coloring or marking certain object structures
and details that do not have their own fluores-
cence with a fluorescent dye. One such dye is
green fluorescent protein (GFP), which is used in
molecular biology.
We recommend that you first familiarize
yourself with the controls of the Leica MZ16 F in
transmitted light without the presence of fluores-
cence illumination.
After switching on the high-pressure
mercury lamp, you need to adjust the burner
immediately see User Manual M2-216-1).
4.1.2 Functional principle
With the Leica MZ16 F fluorescence stereomicro-
scope, entire fluorescent specimens can be
examined three-dimensionally and unprepared,
manipulated, sorted and documented. With a fully
apochromatic optics system, manual 16:1 zoom
and built-in double iris aperture, the Leica MZ16 F
is the high-performance stereomicroscope for
Familiarize yourself with the functional elements
of the Leica MZ16 F such as the binocular tube,
eyepieces, zoom magnification changer, focusing
drive, stands etc. by referring to User Manual
M2-105-0 for Leica M stereomicroscopes. This
manual describes all settings of the stereomicro-
fluorescence applications. The patented, separate scope in detail. The following describes only the
®
TripleBeam light path* for fluorescence illumina-
functions that are specific to the Leica MZ16 F
with fluorescence system.
®
tion and the patented FLUOIII filter system* pro-
vide highest-quality fluorescence images. The very
bright high-pressure mercury burner and specially
selected filter sets enable you to distinguish the
finest structures and enhance the quantity of infor-
mation for incident light fluorescence.
®
The FLUOIII filter system consists of a rapid filter
changer for barrier and excitation filters and a fil-
ter insert for a filter holder with your choice of fil-
ter. A total of four filter sets can be used in the
rapid filter changer. The filter sets are labeled (see
table 22). An empty filter cartridge is also available
for individual filter combinations. Each filter set
includes two barrier filters to the visual beam
paths and an excitation filter to the illumination
beam path.
* Patented in EP (CH, DE, FR, GB),
other patents applied for.
18
Leica MZ16 F – Operation
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5.1.1 Observation without fluorescence
5.1.2 Double iris diaphragm
®
You can use the built-in double iris diaphragm to
adjust the depth of field individually. When doing
so, however, note that when you increase the
depth of field,
– the brightness decreases, and therefore the
exposure times for photography increase,
– the resolution decreases.
The FLUOIII filter system includes a UV shutter
that can be used to close the illumination beam
path. This feature protects the mercury lamp,
which should not be switched on and off too fre-
quently. If, for instance, you need to interrupt your
work, you do not have to switch off the high-pres-
sure mercury burner to preserve sensitive speci-
mens from being burned out or bleached out.
Switching the high-pressure mercury burner on
and off frequently decreases its life and causes
delays, as the lamp can only be switched back on
after it has cooled. Instead, you can close the
UV shutter and reopen it when you return to your
workplace.
̈ Adjust the double iris diaphragm as necessary
using the knurled ring (8.1).
Close the UV shutter:
– if you do not want to view the specimen with
fluorescence illumination, but with transmitted
light only.
– if you are taking a short break from work.
̈ Close the illumination beam path using the
switch (7.1).
1
1
Fig. 7
Fig. 8
1 On/off switch for UV shutter
1 Double iris diaphragm
Leica MZ16 F – Functions
19
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5.2 Commissioning the fluorescence
system
5.3 Rapid filter changer
The rapid filter changer must be equipped with
four filter holders at all times.
After switching on the high-pressure mer-
cury lamp, you must adjust the burner immedi-
ately (see User Manual M2-216-1). Do not switch
on the supply unit until you can safely operate the
Leica MZ16 F.
If you are using fewer than four filter hold-
ers with fluorescence filters, always insert the
included simple filter holders into the available
positions. Otherwise there is a risk of danger to
the eyes from direct UV radiation (see page 16).
Follow all safety instructions when
handling mercury lamps and the lamp housing!
̈ Switch on the supply unit (see User Manual
M2-216-1).
If at all possible, avoid touching the filters
in the filter sets in order to avoid fingerprints.
Wait for 2 to 3 minutes.
̈ Adjust the burner in accordance with User
If you touch them, clean the filters using a soft,
lint-free cloth and pure alcohol.
Manual M2-216-1.
̈ Insert a filter set into the rapid filter changer
such that the label (e.g. GFP) is upright and leg-
ible on the right side.
For observation with fluorescence light,
always position the aperture protection correctly
to protect the observer (page 16).
•
Ensure that the contour of the filter set matches
the contour of the microscope.
̈ Rotate the filter set by hand until it latches into
place and insert the next filter set.
Insert a total of four filter sets or the included sim-
ple filter holders into the empty positions.
2
1
Fig. 9 Filter changer, equipped with filter holders
1 Filter sets
2 Filter slide for filter holder with individual filters
20
Leica MZ16 F – Functions
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6. Work flow
̈ Switch on the supply unit (page 15).
Wait for 2 to 3 minutes.
̈ Close the illumination beam path using the
switch (10.1).
̈ Correctly set the binocular tube, eyepieces and
working distance in accordance with the User
Manual M2-105-0.
̈ Observe the specimen at the lowest magnifica-
tion level first.
This provides a better overview and allows you
to find areas that are of interest more easily.
•
̈ Refocus as necessary.
̈ Observe the details at a higher magnification
level.
̈ Adjust the depth of field as necessary using the
knurled ring (11.1).
̈ Open the illumination beam path using the
switch (10.1).
̈ Rotate the desired filter set into the beam path.
̈ Observe the specimen at the desired level of
magnification.
1
1
Fig. 10
Fig. 11
Leica MZ16 F – Work flow
21
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7. Appendix
7.1 Filter sets
Filter sets
GFP
Excitation filter
Barrier filter
Designation
GFP1
425/60 nm (395–455 nm) 480 LP
480/40 nm (460–500 nm) 510 LP
GFP Plus
GFP Plants
UV
GFP2
470/40 nm (450–490 nm) 525/50 nm (500–550 nm) GFP3
360/40 nm (340–380 nm) 420 LP
425/40 nm (405–445 nm) 460 LP
470/40 nm (450–490 nm) 515 LP
546/10 nm (541–551 nm) 590 LP
UV
V
Violet
Blue
B
Green
CFI
G
436/20 nm (426–446 nm) 480/40 nm (460–500 nm) CFI
510/20 nm (500–520 nm) 560/40 nm (540–580 nm) YFP
YFP
Texas Red
DsRED
CY5
560/40 nm (540–580 nm) 610 LP
TXR
545/30 nm (510–560 nm) 620/60 nm (590–650 nm) DSR
620/60 nm (590–650 nm) 700/75 nm (663–737 nm) CY5
555/60 nm (530–580 nm) 610/75 nm (573–648 nm) CY3
CY3
22
Leica MZ16 F – Appendix
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7.2 Technical data
Optics carrier
Leica MZ16 F
Microscope type
stereo fluorescence microscope with apochromatic
®
optics system, patented TripleBeam third beam path, patented
®
FLUOIII fluorescence filter system, and manual functions
100% apochromatic
optics system,
Specific surface
CMO (Common Main Objective) design with two parallel
beam paths plus a third beam path for fluorescence, lead-free
11
2×10 ohms/square, discharge time <2 seconds
Resistance (housing)
Motorized functions
Manual functions
from 1000V to 100V, antistatic
motorized focus (optional)
zoom, filter changer, UV shutter, double iris diaphragm
manual
Control system
Double iris aperture for balancing
the depth of field
installed, manual
Fluorescence system
Illumination beam path
Leica MZ16 F
2 observation / 1 illumination beam path for intensive
fluorescence illumination, coupling to the zoom optics
horizontally integrated quick changer for four filter sets,
insert for individual filters, UV shutter can be activated/deacti-
vated
®
TripleBeam
®
FLUOIII filter system
Fluorescence filter
Filter sets
excitation filter and barrier filter on one filter holder
GFP, GFP Plus, GFP Plants, UV, violet, blue, green, CFI, YFP, TXR,
DSR, CY3, CY5, filter holder for empty filter positions for protec-
tion from UV radiation
Filter holder without transponder
Light source
for transmitted light or incident light observation without
fluorescence
high-intensity mercury lamp 50 or 100W, lamp housing 106z,
chromatically corrected collector, focusable, centerable lamp
mount
UV aperture protection, UV barrier filter, UV seal, stray-light
protection for lamp housing, filter holder for empty filter posi-
tions
Safety measures
23
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Leica MZ16 F
Zoom
Optical Data
16:1 manual, apochromatic
Selectable zoom positions
for repetitive tasks
Performance with standard optics
(1× objective/10× eyepieces)
– Zoom range
Click-stops at 0.71 | 1 | 1.6 | 2 | 2.5 | 3.2 | 4 | 5 | 6.3 | 8 | 10 | 11.5
7.1× – 115×
420 Lp/mm
0.14
– Resolution
– Numerical aperture
– Field of view
Maximum values
∅ 29.6mm – 1.8mm
(based on optics combination)
– Magnification
920×
– Resolution
840 Lp/mm
– Visible structural width
– Numerical aperture
– Field of view
Optical accessories
Objectives, lead-free
0.6 micron
0.28
∅ 59mm
Leica MZ16 F
Planapochromatic: 1× (NA = 0.141), 2× (NA = 0.282), 1.6×, 0.63×
Planachromatic: 1×, 0.5×, 0.8× with long focal length
55mm (Planapo 1×), 19mm (Planapo 1.6×), 15mm
(Planapo 2×), 97mm (Planapo 0.63×), 60mm (Plan 1×),
112mm (Plan 0.8×), 135mm (Plan 0.5×)
Working distances
®
Binocular tube, ergonomic
Apochromatic ErgoTube 10° – 50° with synchronous interpupil-
®
lary distance adjustment, various ErgoModules (optional)
®
®
ErgoTube and ErgoModule are registered with the "United
States Patent and Trademark Office".
55mm – 75mm
Eyebase (interpupillary distance)
Ergonomic wide-field eyepieces
for persons wearing glasses
High field number, 10×/21, 16×/14, 25×/9.5, 40×/6×,
distortion-free, attachable eyepieces for protection
from infections
24
Leica MZ16 F – Appendix
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Stands, illuminators
Leica MZ16 F
Transmitted light stand
HL RC™ high-performance base with innovative contrast meth-
ods, stands for bright field and bright/dark field
With stage plate, black/white
Incident light stand
Stand for large objects
Motor focus
Swinging arm stand, stage clamp stand, universal stand
With 300mm or 500mm column, control via hand switch, foot
switch or PC
Manual focusing drive
Stages
Coarse/fine focus, adjustable ease of movement, with 300mm or
500mm column
Leica MATS heating stage thermocontrol system, gliding stage,
polarization
Large selection of cold light sources
Illuminators
Various accessories
Video system
Leica IC A
Photography
– Digital high-performance camera systems for fluorescence
– Leica MPS60 modular photomicrographic system with data
back
– SLR camera system with data back
Software
For image grabbing, archiving, processing and analysis: Leica
Image Manager with Image Overlay and Multifocus modules,
FW4000, QWin
Measurement
Various measurement graticules (measurement, counting)
Leica MZ16 F – Appendix
25
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Leica MZ16 F
Weights
Technical Data
– Leica MZ16 F optics carrier
– Lamp housing
– Filter set
– Planapochromatic objective 1×
2.5 kg
20 g
0.74 kg
–
Focusing drive, coarse/fine, with column 1.15 kg
– Microscope carrier
For indoor use only.
– Supply voltage
– Frequency
0.495 kg
ebq 100 supply unit
90–250 V~
50–60 Hz
– Power consumption
– Fuses
– Ambient temperature
– Relative humidity
155 VA max.
2×T2A (IEC 127)
15 – 35°C
maximum 80%
up to 30°C
II
– Overvoltage category
– Degree of pollution
(see instructions provided)
– Resolution
– Input voltage
– Input frequency
– Input current
Column with motorized focus
on incident light or transmitted light
basis, type MST31
2 microns
85 to 264 VAC
47 to 440 Hz
max. 0.80 A at 115 VAC
max. 0.50 A at 230 VAC
30V
– Operating voltage
– Operating temperature
– Storage temperature
– Maximum mechanical loading
maximum load
+10°C to +40°C
–20°C to +55°C
12 kg
Focusing drive, coarse/fine
<15 kg
– 1 micron resolution at 5 kg load
– 2 micron resolution at 10 kg load
26
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7.3 Optical data
1× Plan
1× Planapo
0.5× Plan
0.63× Planapo
0.8× Plan
1.6× Planapo
2× Planapo
Objectives
Working distances in mm
60 Plan
55 Planapo
135 Plan
97 Planapo
112 Plan
19 Planapo
15 Planapo
Eyepieces
0.71
0.8
1
7.1
8
29.6
26.3
21
3.5
4
59.1
52.5
42
4.5
5.1
47
5.7
6.4
8
37
11.4
12.8
16
18.5
16.4
13.1
10.5
8.2
14.2
16
14.8
13.1
10.5
8.4
6.6
5.3
4.2
3.3
2.6
2.1
1.7
1.3
1.1
0.9
41.2
32.8
26.3
20.6
16.4
13.1
10.2
8.2
32.8
26.3
21
10
5
6.4
20
1.25
1.6
2
12.5
16
16.8
13.1
10.5
8.4
6.3
8
33.3
26.3
21
8
10
20
25
10.2
12.8
16
12.8
16
16.4
13.1
10.5
8.2
25.6
32
32
20
10
6.6
40
2.5
25
12.5
16
16.8
13.1
10.5
8.4
20
40
5.3
50
10×/21B
3.2
32
6.6
20.5
25.6
32
25.6
32
51.2
64
4.1
64
4
5
40
5.3
20
6.6
3.3
80
50
4.2
25
6.6
40
5.3
80
2.6
100
126
160
200
230
6.3
8
63
3.3
31.5
40
6.7
40.3
51.2
64
5.2
50.4
64
4.2
100.8
128
160
184
2.1
80
2.6
5.3
4.1
3.3
1.6
10
11.5
100
115
2.1
50
4.2
3.3
80
2.6
1.3
1.8
57.5
3.7
72.5
2.9
92
2.2
1.1
0.71
0.8
1
11.4
12.8
16
19.7
17.5
14
5.7
6.4
8
39.4
35
7.2
8.2
31.3
27.3
22
9.1
10.2
12.8
16
24.6
22
18.2
20.5
25.6
32
12.3
10.9
8.8
7
5.5
4.4
3.5
2.7
2.2
1.8
1.4
1.1
0.9
0.8
22.8
25.6
32
9.8
8.8
7
28
10.2
12.8
16.4
20.5
25.6
32.8
41
17.5
14
1.25
1.6
2
20
11.2
8.8
7
10
22.4
17.5
14
11.2
8.8
7
5.6
4.4
3.5
2.8
2.4
17.5
13.7
10.9
8.8
40
5.6
4.4
3.5
2.8
2.2
1.8
1.4
1.1
0.9
0.7
0.6
25.6
32
12.8
16
20.5
25.6
32
10.9
8.8
7
5.5
4.4
3.5
2.8
2.2
1.8
1.5
41
51.2
64
80
102.4
128
160
201.6
256
320
368
51.2
64
2.5
40
5.6
4.4
3.5
2.8
2.2
1.8
1.4
1.2
20
16×/14B
3.2
51.2
64
25.6
32
6.8
41
51.2
64
80.6
102.4
128
147.2
81.9
102.4
128
4
5
5.5
80
40
51.2
64.5
81.9
102.4
115.9
4.4
6.3
8
100.8
128
160
184
50.4
64
3.5
161.3
204.8
256
2.7
10
11.5
80
2.2
92
1.9
294.4
0.71
0.8
1
17.7
20
13.4
11.9
9.5
7.6
5.9
4.8
3.8
3
8.9
10
26.8
23.8
19
11.1
12.8
16
21.2
18.6
14.8
11.9
9.3
7.4
5.9
4.6
3.7
3
2.4
1.9
1.5
1.3
14.2
16
16.7
14.8
11.9
9.5
7.4
5.9
4.8
3.7
3
2.4
1.9
1.5
1.2
1
28.3
32
8.4
7.4
5.9
4.8
3.7
3
2.4
1.9
1.5
1.2
0.9
0.7
0.6
0.5
35.4
40
6.7
5.9
4.8
3.8
3
2.4
1.9
1.5
1.2
1
0.8
0.6
0.5
0.4
25
12.5
15.6
20
20
40
50
1.25
1.6
2
31.3
40
15.2
11.9
9.5
7.6
5.9
4.8
3.8
3
20
25
50
62.5
80
25.6
32
32
64
50
25
40
80
100
125
160
200
250
315
400
500
575
2.5
62.5
80
31.3
40
40
50
100
128
160
200
252
320
400
460
25×/9.5B
3.2
51.2
64
64
4
5
100
125
157.5
200
250
287.5
2.4
1.9
1.5
1.2
1
50
80
62.5
78.8
100
125
143.8
80
100
126
160
200
230
6.3
8
100.8
128
160
181.1
2.4
1.9
1.7
10
11.5
0.8
0.71
0.8
1
28.4
32
8.4
7.5
6
14.2
16
16.9
15
12
9.6
7.5
6
17.9
20.5
25.6
32
13.4
11.7
9.4
7.5
5.9
4.7
3.8
2.9
2.3
1.9
1.5
1.2
0.9
0.8
22.7
25.6
32
10.5
9.4
7.5
6
45.4
51.2
64
5.3
4.7
3.8
3
2.3
1.9
1.5
1.2
0.9
0.8
0.6
0.5
0.4
0.3
56.8
64
4.2
3.8
3
40
20
80
1.25
1.6
2
50
4.8
3.8
3
25
40
80
100
128
160
200
256
320
400
504
640
800
920
2.4
1.9
1.5
1.2
0.9
0.8
0.6
0.5
0.4
0.3
0.3
64
32
41
51.2
64
80
102.4
128
160
201.6
256
320
368
4.7
3.8
3
102.4
128
80
40
51.2
64
2.5
100
128
160
200
252
320
400
460
2.4
1.9
1.5
1.2
1
0.8
0.6
0.5
50
4.8
3.8
3
160
40×/6B
3.2
4
64
81.9
102.4
128
2.3
1.9
1.5
1.2
0.9
0.8
0.6
204.8
256
80
5
100
126
160
200
230
2.4
1.9
1.5
1.2
1
320
6.3
8
161.3
204.8
256
403.2
512
10
11.5
640
289.8
736
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27
7.4 Dimensions
Dimensions in mm
28
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Tel. +33 1 4732 8585
Tel. +49 6251 1360
Tel. +39 02 57 486 1
Fax +33 1 4732 8586
Fax +49 6251 136 155
Fax +39 02 5740 3273
Germany:
Italy:
Japan:
Korea:
Tokyo
Seoul
Tel. +81 3 543 596 09 Fax +81 3 543 596 15
Tel. +82 2 514 6543 Fax +82 2 514 6548
Netherlands:
Portugal:
Singapore:
Spain:
Sweden:
Switzerland:
Rijswijk
Lisbon
Tel. +31 70 41 32 130 Fax +31 70 41 32 109
Tel. +35 1 213 814 766 Fax +35 1 213 854 668
●
Medical Equipment
Tel. +65 6 77 97 823
Tel. +34 93 494 9530
Tel. +46 8 625 45 45
Fax +65 6 77 30 628
Fax +34 93 494 9532
Fax +46 8 625 45 10
Innovative technologies in our surgical micro-
scopes offer new therapeutic approaches in
microsurgery.
Barcelona
Sollentuna
Glattbrugg
Tel. +41 44 809 34 34 Fax +41 44 809 34 44
Tel. +44 1908 246 246 Fax +44 1908 609 992
United Kingdom: Milton Keynes
USA:
●
Semiconductor Equipment
Bannockburn/Illinois Tel. +1 800 248 0123
Fax +1 847 405 0164
Our automated, leading-edge measurement and
inspection systems and our E-beam lithography
systems make us the first choice supplier for
semiconductor manufacturers all over the world.
and representatives of Leica Microsystems
in more than 100 countries.
In accordance with the ISO 9001 certificate, Leica Microsystems (Switzerland) Ltd,
Business Unit Stereo & Macroscope Systems has at its disposal a management system
that meets the requirements of the international standard for quality management. In
addition, production meets the requirements of the international standard ISO 14001 for
environmental management.
Leica Microsystems (Switzerland) Ltd Telephone +41 71 726 33 33
Stereo & Macroscope Systems
CH-9435 Heerbrugg
Fax +41 71 726 33 99
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