Leica VT1000A
Vibratome
Operating Manual
Leica VT1000A
V1.1 English - 01/2010
Always keep this manual with the instrument.
Read carefully before working with the instrument.
Download from Www.Somanuals.com. All Manuals Search And Download.
NOTE
The information, numerical data, notes and val-
These are determined only by the contract pro-
visions agreed between ourselves and our cus-
tomers.
ue judgments contained in this manual repre-
sent the current state of scientific knowledge
and state-of-the-art technology as we under-
stand it following thorough investigation in this
field.
We are under no obligation to update the pres-
ent manual periodically and on an ongoing basis
according to the latest technical developments,
nor to provide our customers with additional
copies, updates etc. of this manual.
To the extent permitted in accordance with the
national legal system as applicable in each indi-
vidual case, we shall not be held liable for erro-
neous statements, drawings, technical illustra-
tions etc. contained in this manual. In particular,
no liability whatsoever shall be accepted for any
financial loss or consequential damage caused
by or related to compliance with statements, in-
structions or other information in this manual.
Statements, drawings, illustrations and other in-
formation regarding the contents or technical
details of the present Operating Manual are not
to be considered warranted characteristics of
our products.
Leica reserves the right to change technical
specifications as well as manufacturing pro-
cesses without prior notice. Only in this way is
it possible to continuously improve the technol-
ogy and manufacturing techniques used in our
products.
This document is protected under copyright
laws. All copyrights to this documentation are
held by Leica Biosystems Richmond LLC.
Any reproduction of text and illustrations (or
of any parts thereof) by means of print, photo-
copy, microfiche, web cam or other methods—
including any electronic systems and media—
requires express prior permission in writing by
Leica Biosystems Richmond LLC.
For the instrument serial number and year of
manufacture, please refer to the nameplate at
the rear side of the instrument.
© Leica Biosystems Richmond LLC
Published by:
Leica Biosystems Richmond, LLC
5205 Route 12P.O. Box
528 Richmond, IL 60071
United States of Amerika
Telefon: +1 800 225-3035
Fax:
+1 815 678-2216
Leica VT1000 A
3
Download from Www.Somanuals.com. All Manuals Search And Download.
Contents
1.
2.
Important Notes ........................................................................................................................................................ 5
Safety.....................................................................................................................................................................................6
2.1
Safety notes...................................................................................................................................................................... 6
2.2
Warnings........................................................................................................................................................................... 6
3.
4.
Instrument Components and Specifications ....................................................................................................... 9
3.1
3.2
Overview — instrument components.......................................................................................................................... 9
Technical data ............................................................................................................................................................... 10
Setup the Instrument.............................................................................................................................................. 12
4.1
4.2
4.3
4.4
Standard delivery .......................................................................................................................................................... 11
Installation site requirements...................................................................................................................................... 12
Unpacking and setting up the instrument ................................................................................................................. 13
Connection to power supply system.......................................................................................................................... 14
4.4.1 Checking the performance requirements ................................................................................................................. 14
4.4.2 Connecting to the power supply................................................................................................................................. 15
4.5
4.6
4.7
Connecting the foot switch.......................................................................................................................................... 16
Assembling the "deluxe specimen tray"..................................................................................................................... 17
Assembling the specimen clamp................................................................................................................................ 19
4.7.1 Inserting the round specimen tray ............................................................................................................................. 20
4.7.2 Assembling the directional specimen holder fixture............................................................................................... 21
4.8
Assembling the magnifier ............................................................................................................................................ 22
5.
Operation.................................................................................................................................................................. 23
5.1
Operating elements and their functions.................................................................................................................... 23
5.1.1 The control panel...............................................................................................................................................................
5.1.2 The operating elements................................................................................................................................................ 23
5.2
Adjustment options on the instrument....................................................................................................................... 28
5.2.1 Adjusting the blade presentation angle..................................................................................................................... 28
5.2.2 Adjusting the specimen and blade............................................................................................................................. 29
5.2.3 Adjustable sectioning window.................................................................................................................................... 31
5.2.4 Adjusting the specimen height.................................................................................................................................... 33
5.3
Sectioning....................................................................................................................................................................... 34
5.3.1 General instructions...................................................................................................................................................... 36
5.3.2 Inserting the specimens............................................................................................................................................... 37
5.3.3 Selecting and inserting the blade............................................................................................................................... 39
5.4
5.5
5.6
5.7
Specimen preparation.................................................................................................................................................. 41
Preparing the specimen bath...................................................................................................................................... 42
Conventional sectioning............................................................................................................................................... 43
Removing the sections ................................................................................................................................................. 44
6.
7.
Cleaning and Maintenance .................................................................................................................................. 45
6.1
6.2
6.3
Cleaning the instrument ............................................................................................................................................... 45
Maintenance instructions............................................................................................................................................ 47
Replacing components................................................................................................................................................. 48
6.3.1 Fuse.................................................................................................................................................................................. 48
6.3.2 LED Light ......................................................................................................................................................................... 48
Optional Accessories ............................................................................................................................................ 49
7.1
7.2
Ordering information..................................................................................................................................................... 49
Accessories.................................................................................................................................................................... 50
8.
Warranty and Service............................................................................................................................................ 55
Operating Manual V 1.1 – 01/2010
4
Download from Www.Somanuals.com. All Manuals Search And Download.
1. Important Notes
Symbols in the text and their meanings
Intended use
The Leica VT1000 A is used for sectioning in the
fields of medicine, biology and industry, and is
especially designed for sectioning fixed or un-
fixed fresh tissue in a buffer solution.
Dangers, warnings and cautions
appear in a gray box and are marked
by a warning triangle.
The instrument must be installed according to
the directions in this Operating Manual.
Notes,
i.e. important information for the user,
are highlighted in gray and marked by
Any other use of the instrument is considered
improper!
the
symbol.
Numbers in parentheses refer to item
numbers in illustrations or to the illus-
trations themselves.
Instrument Type
(5)
All information contained in this Operating Man-
ual applies solely to the instrument type listed
on the cover page.
(Fig.3)
A nameplate indicating the instrument serial
number is attached to the rear side of the in-
strument.
Qualification of personnel
• The Leica VT1000 A may be operated by
For all inquiries please specify
Instrument Type
trained laboratory personnel only.
• All laboratory personnel designated to op-
erate the Leica instrument must read this
Operating Manual carefully and must be
familiar with all technical features of the in-
strument before attempting to operate it.
Serial Number
Fig. 1
Leica VT1000 A
5
Download from Www.Somanuals.com. All Manuals Search And Download.
2. Safety
The safety and caution notes in this chapter must be observed at all times.
Be sure to read these notes even if you are already familiar with the operation and use of other
Leica products.
2.1 Safety notes
This instrument has been built and inspected
in accordance with the safety regulations for
electrical measuring, control, regulating and
laboratory devices.
This Operating Manual contains important in-
structions and information regarding the oper-
ational safety and maintenance of the instru-
ment.
To maintain this condition and ensure safe op-
eration, the user must observe all notes and
warnings contained in this Operating Manual.
The Operating Manual is an important part of
the product, and must be read carefully prior to
startup and use and must always be kept near
the instrument.
The safety precautions listed below are intend-
ed to prevent injuries of the operating person-
nel, damage of the instrument or impairment of
the instrument's performance.
For current information on applicable
standards, please refer to the instru-
ment’s CE declaration and visit:
If additional requirements on accident
prevention and environmental protec-
tion exceeding the scope of this Op-
erating Manual are imposed by laws/
regulations of the country of opera-
tion, this Operating Manual must be
supplemented by appropriate instruc-
tions to ensure compliance with such
requirements.
The protective devices located on the instrument and the accessories must not be removed or
modified. The instrument must only be opened and repaired by service technicians authorized
by Leica.
2.2 Warnings
The safety devices installed in this instrument by the manufacturer only constitute the basis
for accident prevention. Operating the instrument safely is, above all, the responsibility of the
owner, as well as the designated personnel who operate, service or clean the instrument.
To ensure trouble-free operation of the instrument, make sure to comply with the following
instructions and warnings.
Operating Manual V 1.1 – 01/2010
6
Download from Www.Somanuals.com. All Manuals Search And Download.
2. Safety
Warnings – Markings on the instrument itself
•
Warning labels on the instrument marked with a warning triangle indicate that the correct
operating instructions (as defined in this Operating Manual) must be followed when operating
or replacing the item marked. Failure to adhere to these instructions may result in an accident,
personal injury, damage to the instrument or accessory equipment.
•
Observe the warning messages on the rear side of the instrument (Fig. 2).
Only the components described in the section on "Replacing components" may be replaced by
the operator.
15
4
Fig. 2
Warnings – Transport and installation
•
•
Once unpacked, the instrument may be transported only in an upright position.
Never lift or transport the instrument by holding it by the blade holder or setting wheel for the
section thickness.
•
Theinstrumentmustbesetupsothatthemainpowerswitchontheleftrearsideoftheinstrument
(item 14 in Fig. 2) is easily accessible at any time.
Warnings – Maintenance and cleaning
•
•
The instrument may be opened by authorized service personnel only.
Always disconnect the power plug before opening the instrument!
•
Turn the instrument off using the power switch and disconnect the power plug before replacing
the fuses. The use of fuses other than those provided is not permitted.
•
•
Before each cleaning, remove the disposable blade or knife!
Do not use any solvents containing acetone or xylene for cleaning! Ensure that liquids do not
enter the interior of the instrument during cleaning.
•
•
Do not clean the magnifier using cleaners that contain solvents, as the lens is made of acrylic.
When using detergents, please comply with the safety precautions of the manufacturer and the
laboratory regulations!
Leica VT1000 A
7
Download from Www.Somanuals.com. All Manuals Search And Download.
2.
Safety
Warnings – Operating the instrument
•
Take care when handling disposable blades and sapphire blades. The cutting edge is extremely
sharp and can cause serious injuries!
Always wear work safety shoes and safety gloves!
•
•
Always clamp the specimen block BEFORE clamping the blade/knife.
If making adjustments or configuring settings in the specimen area, take the blade out of the
holder in order to prevent cutting injuries from accidental contact with the blade.
Caution! Risk of infection when working with fresh tissue or with material where an infection
cannot be excluded!
•
•
There is a fire hazard from an uncovered magnifier! Cover or remove the magnifier when the
instrument is unsupervised.
•
The cyanoacrylate adhesive in the accessories package included for the tissue sample adheres
very quickly to human skin. Avoid contact with fingers when using the adhesive.
Proper handling
•
•
Always be exceptionally careful when handling theblades or knife! Always make sure tohandle
a blade in a way that cannot cause you injury. If possible, grasp the blade using a pincers or
hold it by its blunt ends only.
Do not leave open blades lying around after removal. When disposing of used blades, apply
common "Scotch" tape over the cutting edge or wrap the entire blade with paper.
All appropriate safety precautions must be met to avoid the risk of infection!
Wearing safety gloves, a mask and safety goggles—in accordance with the 'Working with
Substances that Pose a Health Risk' guidelines—is absolutely mandatory.
Caution! Risk of infection when working with fresh tissue or with material where an infection
cannot be excluded!
If, despite this, you come into direct contact with the cyanoacrylate adhesive provided, first
allow the adhesive to air dry, then wipe it off with a towel soaked in acetone. When uninten-
tional bonding of skin occurs, separate by a "peeling" (rather than pulling) action after applying
acetone to the bond area.
•
•
•
•
Operating Manual V 1.1 – 01/2010
8
Download from Www.Somanuals.com. All Manuals Search And Download.
3. Instrument Components and Specifications
3.1 Overview — instrument components
LED Light Assembly
Magnifier
Specimen Bath
Vibratome tray
Section Thickness Knob
Adjustment knobs
for the sectioning
window
Control panel
Hazard warning
Rear view of the instrument
Nameplate
Fuses
Connection for
footswitch
Power switch
Connection
Power cable
Drainage valve
for liquid from the
Vibratome tray
Instrument feet
Foot switch
ON/OFF
Fig. 3
Leica VT1000 A
9
Download from Www.Somanuals.com. All Manuals Search And Download.
3.
Instrument Components and Specifications
3.2 Technical data
General
Approvals:
The instrument-specific approval marks are
located on the identification label.
+10°C to +35°C
Operating temperature range:
Relative humidity:
max. 80% non-condensing
Operating temperature range during storage: + 5°C to +55°C
Storage humidity:
< 80%
Microtome
Max. section travel length:
Sectioning Speed :
Cutting head retraction:
Blade amplitude:
Total specimen stroke:
Maximum specimen size:
Total angle adjustment:
Tilt, specimen holder:
0 - 40 mm
0 - 2.0 mm/s continuously adjustable
c 5-7 mm/s non-adjustable
0 - 3mm, +/- 1.5mm continuously adjustable
15 mm
33 x 40 mm
0° to 50° continuously adjustable
-5° - +5° in one axis
Electrical data
The instrument is supplied for operation with alternating current in two voltage variants and
for two different frequencies (50 Hz and 60 Hz). For the corresponding product number, refer to
the table on page 14.
Voltage:
110V AC +/- 15 % 220V AC +/- 15%
Max. power consumption: 1A
0.5A
Frequency:
50 Hz or 60 Hz (for both voltage variants)
Dimensions and weights
Width:
Depth:
Height:
280mm
460mm
310mm
Weight (without accessories):
15.5kg
Operating Manual V 1.1 – 01/2010
10
Download from Www.Somanuals.com. All Manuals Search And Download.
4. Setup the Instrument
4.1 Standard delivery
The standard equipment of the Leica VT1000 A is available in 3 variants
and includes the following parts:
1. STARTER PACKAGE
1 Specimen Tray (includes V-block adapter)................................... 39053744
1 Specimen Blocks, (3 blocks)............................................................ 39053747
2 Specimen Adhesive........................................................................... 39053986
2 Feather Blades, (20 blades).............................................................. 39053234
1 Instrument Cover ............................................................................... 39053746
1 Blade Angle Indicator..................................................................... 392043401
1 2x Magnifier....................................................................392000200MAGREPL
1 Power Cord (110V)*......................................................................... 392000101
1 Plug Connector, Quick Connect (bath drain) .............................. 393104023
2 Replacement Fuse........................................................................... 392000109
1 5/32", Short Arm Hex Key.............................................................. 3997000002
1 Manual and DVD............................................................................ 3996000107
2. PROFESSIONAL PACKAGE
1 Deluxe Specimen Bath ..................................................................... 39053745
1 Rotating Stage Assembly ................................................................. 39053760
1 Specimen Blocks, (3 blocks)............................................................ 39053747
1 Foot Pedal ........................................................................................... 39053300
1 Sapphire Knife.................................................................................... 39053237
3 Specimen Adhesive........................................................................... 39053986
3 Feather Blades (30 blades)............................................................... 39053234
1 Instrument Cover ............................................................................... 39053746
1 Blade Angle Indicator..................................................................... 392043401
1 2x Magnifier....................................................................392000200MAGREPL
1 Power cable (110V)*........................................................................ 392000101
1 Plug Connector, Quick Connect (bath drain) .............................. 393104023
2 Replacement Fuse........................................................................... 392000109
1 5/32", Short Arm Hex Key.............................................................. 3997000002
1 Manual and DVD............................................................................ 3996000107
Leica VT1000 A
11
Download from Www.Somanuals.com. All Manuals Search And Download.
4. Setup the Instrument
3. PROFESSIONAL GOLD PACKAGE
1 Rotating Stage Assembly ................................................................. 39053760
1 Foot Pedal ........................................................................................... 39053300
1 Sapphire Knife.................................................................................... 39053237
4 Specimen Adhesive........................................................................... 39053986
5 50 Feather Blades.............................................................................. 39053234
1 Instrument Cover ............................................................................... 39053746
1 Blade Angle Indicator..................................................................... 392043401
1 2x Magnifier....................................................................392000200MAGREPL
1 Power Cord (110V) *........................................................................ 392000101
1 Plug Connector, Quick Connect (bath drain) .............................. 393104023
2 Replacement Fuse........................................................................... 392000109
1 5/32", Short Arm Hex Key.............................................................. 3997000002
1 Manual and DVD............................................................................ 3996000107
* only one cord included; determined at time of order
1 Power Cord (220V)........................................................................... 392130701
1 Power Cord (AU).............................................................................. 392000122
1 Power Cord (UK) .............................................................................. 392000120
The accessories ordered are included in a separate box.
Carefully check the delivery against the packing list and the deliv-
ery note. Should you find any discrepancies, please contact your
Leica sales office without delay.
4.2 Installation site requirements
• Stable, vibration-free laboratory bench with horizontal, even stage
plate; practically vibration-free floor.
• No other instruments nearby which might cause vibrations.
• Room temperature consistently between + 10°C and + 35°C.
• The instrument is suitable for operation in enclosed rooms only.
Operating Manual V 1.1 – 01/2010
12
Download from Www.Somanuals.com. All Manuals Search And Download.
4. Setup the Instrument
4.3 Unpacking and setting up the instrument
•
•
First check the shipment for external damages upon arrival.
If it is evident that the shipment was damaged during transport, please make a claim to the
carrier immediately.
•
Ensure that the instrument is standing on a work surface that is as free of vibrations as possible.
The instrument must be set up the instrument so that the left rear side of the instrument (item
15 in Fig. 7) is easily accessible at any time.
1. Remove the lid (1) of the transport crate by
unscrewing the 4 Phillips head screws (2).
2. Take out the accessory box (3) and remove
the white packing material (5).
1
2
3. Remove the other accessory cartons.
4. To lift the instrument from the box, hold it on
the left and right of the housing (Fig. 6), lift it
out of the foam cushion of the package and
place it on a stable lab table.
Never lift the instrument by holding
it by its blade holder (6), the setting
wheel (7) for the section thickness or
adjustment knobs (8) for section cutting
window.
Fig. 4
6
7
5
3
8
Fig. 5
Fig. 6
Leica VT1000 A
13
Download from Www.Somanuals.com. All Manuals Search And Download.
4.
Setup the Instrument
4.4 Connection to power supply system
4.4.1 Checking the performance requirements
The Vibratome VT1000 A is available in different versions for operation
with different supply voltage.
Before connecting the instrument to the power supply, check to make
sure that the product number (39 072XXX-X) and the marking for the input
voltage on the rear side of the instrument match in order to ensure that
the correct version for the supply voltage available at the location has
been delivered.
For the assignment of the product number to the respective version, refer
to the following table.
Product number Instrument version
39072018
Leica VT1000 A Starter
LEICA VT1000 A Starter
LEICA VT1000 A Starter,
LEICA VT1000 A Starter
LEICA VT1000 A Professional
LEICA VT1000 A Professional
LEICA VT1000 A Professional
LEICA VT1000 A Professional
LEICA VT1000 A Pro Gold
LEICA VT1000 A Pro Gold
LEICA VT1000 A Pro Gold
LEICA VT1000 A Pro Gold
110V, 60Hz
110V, 50Hz
220V, 50Hz
220V, 60Hz
110V, 60Hz
110V, 50Hz
220V, 50Hz
220V, 60Hz
110V, 60Hz
110V, 50Hz
220V, 50Hz
220V, 60Hz
39072018-1
39072018-2
39072018-3
39072020
39072020-1
39072020-2
39072020-3
39072022
39072022-1
39072022-2
39072022-3
110 V instruments have a max. amperage of 1.0A
220 V instruments have a max. amperage of 0.5A
The product number and all corresponding connection values are
located on the nameplate on the rear side of the instrument.
See Fig. 1, page 5
Operating Manual V 1.1 – 01/2010
14
Download from Www.Somanuals.com. All Manuals Search And Download.
4. Setup the Instrument
4.4.2 Connecting to the power supply
All electrical connections are on the left rear
side of the instrument.
The Leica VT1000A MUST be connect-
ed to a grounded power socket.
The instrument is supplied with a
power cable that is suitable for the
local power supply (socket). Only this
cable may be used to operate the in-
strument!
Do not use an extension cable!
15
16
Fig. 7
Connecting to power supply
Severe damage may occur if the in-
strument is connected to a power sup-
ply voltage other than that to which it
was originally set.
The power supply voltage for the in-
strument is factory preset and CAN-
NOT be altered by the user.
• Make sure that the Vibratome is switched
off:
The main switch (15) on the rear side must
be in the "0"= OFF position.
• Plug the power cable provided into the in-
put socket (16) of the power supply on the
instrument, then into a socket.
Leica VT1000 A
15
Download from Www.Somanuals.com. All Manuals Search And Download.
4. Setup the Instrument
4.5 Connecting the foot switch
The standard scope of delivery of the "Profes-
sional" and "Professional Gold" instrument ver-
sions include a foot switch (18, Fig. 8) that can
be connected to the rear side of the instrument.
18
In SINGLE and AUTO modes, the sectioning pro-
cess can be started and stopped with the foot
switch instead of the DIRECTION switch.
To connect the foot switch, follow these steps:
17
• Route the cable of the foot switch so that the
switch on the floor can be reached easily
with one foot.
20
• Insert the plug (17) of the foot switch into
the socket (19) provided on the rear side of
the instrument. This is possible in one posi-
tion only; it is not possible to connect it the
wrong way.
19
• Then screw the plug and the retaining ring
(20) securely onto the thread of the socket
(19).
Rear side of the
instrument
• Before the foot switch can be used, the small
switch (21) must be moved into the "ON" po-
sition, (Fig. 13).
If the foot pedal switch in the “ON”
position, the movement of the blade
can not be initiated by the “DIREC-
TION” switch.
21
Fig. 8
Operating Manual V 1.1 – 01/2010
16
Download from Www.Somanuals.com. All Manuals Search And Download.
4. Setup the Instrument
4.6 Assembling the "deluxe specimen tray"
The deluxe specimen bath (22, Fig. 11) is assem-
bled inside the standard black specimen bath
(23, Fig. 10). It enables separation of buffer so-
lution and ice preventing dilution of the buffer
while sectioning.
The deluxe specimen bath can be sterilized and
has round specimen discs on which the speci-
mens can be fastened very easily (Fig. 11, page
17)
24
25
The surface of the deluxe specimen bath and
the specimen disks (32) both have an angle
scale so that a repeatable position of the speci-
mens relative to the blade is possible.
26
Fig. 9
• Fasten the u-frame bracket (24, Fig. 9) for the
deluxe specimen bath in the main specimen
bath with the corresponding countersunk
hexagon screw (26) on the round specimen
stage (27).
32
22
23
27
Fig. 10
Fig. 11
Leica VT1000 A
17
Download from Www.Somanuals.com. All Manuals Search And Download.
4.
Setup the Instrument
Assembly of the "deluxe specimen tray" (continued)
• As shown in Fig. 12, attach the u-frame
bracket to the main specimen bath (27, Fig.
10).
• Insert the countersunk screw (26) into the
bore and tighten it using a size 3 Allen key
(25). When tightening, ensure that the fix-
ture (24) is parallel to the front edge of the
Vibratome tray (Fig. 12).
25
26
30
Fig. 12
30
Enlarged detail:
Knurled screw
and clamping
piece for fasten-
ing the deluxe
specimen tray in
the fixture.
30
28
29
Fig. 13
• Insert the deluxe specimen tray into the in-
stalled u-frame bracket so that the slotted
cam screw (28) for fastening the specimen
disks is at the front left (Fig. 13).
• Then screw the specimen bath securely in
place using the clamping pieces (29) and
knurled screws (30) on both sides of the u-
frame bracket.
28
32
• Insert the specimen disc (32) on the surface
provided and clamp it in place by tightening
the slotted cam screw with a screw driver
(28) (Fig. 14).
Fig. 14
Operating Manual V 1.1 – 01/2010
18
Download from Www.Somanuals.com. All Manuals Search And Download.
4. Setup the Instrument
4.7 Assembling the specimen vice assembly
Always remove the blade BEFORE in-
stalling or removing accessories!
• The specimen vice assembly (34, Fig. 16) is
assembled inside the main specimen bath
(23, Fig. 15).
• Insert the cheese head screw (35) into the
bore of the specimen stage (27) and tighten
it using a size 5 Allen key (37, included in the
scope of delivery).
23
27
• When tightening, ensure that the fixture (34)
is parallel to the front edge of the Vibratome
tray (Fig. 17).
Fig. 15
• Insert the specimen block (36) into the ten-
sion clamp and clamp it into place using the
knurled screw (39) (Fig. 18).
36
34
• The specimen to be sectioned is affixed to
the specimen block using the cyanoacrylate
adhesive provided.
• The anterior knurled screw, allows for orien-
tation of the specimen vice assembly, +/- 8
degrees. To change orientation, loosen the
screw and press on either side of the ten-
sion clamp. (refer also to Fig. 22).
35
37
Fig. 16
39
36
38
34
37
Fig. 17
Fig. 18
Leica VT1000 A
19
Download from Www.Somanuals.com. All Manuals Search And Download.
4.
Setup the Instrument
4.7.1 Inserting the round specimen tray
The round specimen tray (45) can be installed
in place of the specimen block. It enables easy
separation of buffer solution and ice that can
accumulate in the main specimen bath.
45
46
Always remove the blade BEFORE in-
stalling or removing accessories!
Fig. 19
To insert it, follow these steps:
• First, insert the V-block adapter (46) into the
tensioning clamp (46) as shown in Fig. 20.
• Position the lower post at the bottom of the
round specimen tray into the V-block adapt-
er (46) so that it can be clamped into place
using the knurled screw (37) (Fig. 21).
34
46
• Hold the specimen tray horizontally and
clamp it into place using the knurled screw
(Fig. 22).
Fig. 20
39
45
46
46
Fig. 21
Fig. 22
Operating Manual V 1.1 – 01/2010
20
Download from Www.Somanuals.com. All Manuals Search And Download.
4. Setup the Instrument
4.7.2 Assembling the rotating stage assembly
The rotating stage assembly allows for simple
position correction of the specimen surface
when the specimen is glued in place.
40
• To do so, first remove the specimen vice as-
sembly (34, Fig. 16) by following the reverse
order of steps as described in Chap. 4.7.
• Unscrew the knurled screw (38) completely.
You can now take the specimen clamp (40)
off of the base plate (39) (Fig. 23).
38
39
• Attach the rotating stage assembly (42) to
the base plate so that the pin (41) fits into the
bore (45) provided.
Fig. 23
• Then, screw the knurled screw (38) into the
thread and tighten it, thus fastening the ro-
tating stage assembly (41) to the base plate
(39).
45
42
38
44
• Install it in the main specimen bath (23) as
described in Chap. 4.7 (Fig. 25).
39
41
43
43
44
Fig. 24
• The specimen to be sectioned (44) is glued
to the round specimen plate using the cy-
anoacrylate adhesive provided.
• To orient the specimen, slightly loosen the
knurled screw (43) on the right side, adjust to
the desired position and retighten the screw.
Fig. 25
Leica VT1000 A
21
Download from Www.Somanuals.com. All Manuals Search And Download.
4.
Setup the Instrument
4.8 Assembling the magnifier
The standard scope of delivery of the instru-
ment includes a 2x magnifier that can be as-
sembled on the lamp carrier.
• Move the lamp carrier (10, in Fig. 27) on the
instrument out by pulling it forwards.
• The bottom of the lamp carrier has two
notches (12), one each on the left and right.
• Install the magnifier (13) so that the two pins
(11) on the left and right fit into the corre-
sponding groove. Push the pins upwards into
the groove as far as they will go as shown in
the enlarged section of Fig. 27.
11
• The magnifier is now securely fastened and
can be moved along with the lamp carrier.
11
10
13
14
Fig. 26
• For precision viewing of the
sectioning process, the
magnifier can be moved
and adjusted on both
arms (14).
Fig. 27
• To remove the magnifier,
follow the reverse order of
steps.
12
Operating Manual V 1.1 – 01/2010
22
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
5.1 Operating elements and their functions
5.1.1 The control panel
DIRECTIONAL SWITCH
Direction of cutting head
Rotary knob
SPEED
Display
Light switch
PAUSE
Switch
Rotary knob
AMPLITUDE
Height adjustment of Section thickness Number of
the specimen stage sections
Sectioning switch
Automatic/Single/
Manual
Fig. 28
5.1.2 The operating elements
PAUSE switch
With this switch, you can temporarily stop the feed movement of the
blade at any place.
The PAUSE switch is useful particularly if you want to adjust the height
of the specimens or remove specimens during a cycle.
Switch position: Upwards ––> Sectioning is interrupted.
Downwards ––> Sectioning is possible.
Fig. 29
Leica VT1000 A
23
Download from Www.Somanuals.com. All Manuals Search And Download.
5.
Operation
The operating elements (continued)
DIRECTION switch
This switch and its three switch positions (upper, center, lower) con-
trol the movement direction of the cutting head.
• Push the switch upwards to start the sectioning cycle. The sec-
tioning blade will advance forward at maximum speed when the
switch is held at its top position.
• As soon as the switch is released, it returns to the center position.
• The blade continues the feed movement with the default values for
SPEED and AMPLITUDE.
• At the end of the sectioning window, the movement direction of
the blade is reversed and the cutting head moves back. Reversing
stops when the rearmost "ready" position is reached.
• During the feed movement, the movement direction can be
reversed at any time by moving the switch to the lower position.
• Likewise, during the retraction movement, you can switch direction
by pushing the switch upwards.
Fig. 30
Switch position:
Upwards --> forwards
Downwards --> backwards
Rotary knob
SPEED
Using this rotary knob, you can change the relative speed of the cut-
ting head (feed movement) if the DIRECTION switch is in the FWD/
AUTO REV position.
• In the zero position (‘0‘), the blade does not move; in the ‘10‘ posi-
tion, it is moved at maximum speed (2 mm/s).
• For the positions in between, the speed is changed proportionally.
Only the feed rate can be changed; this is also possible during
the sectioning process.
The retraction speed is always the same.
Fig. 31
Operating Manual V 1.1 – 01/2010
24
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
The operating elements (continued)
This knob controls the relative amplitude of the lateral excursion of
the sectioning blade vibratory movements.
AMPLITUDE rotary knob
• In the zero position (‘0‘) or during the retraction movement of the
cutting head, the blade does not vibrate.
• The maximum amplitude (2.0 mm) is reached when the rotary knob
is in the ‘10‘ position. At intermediate settings, the amplitude will
vary proportionally.
All configured values are active during the feed movement of
the cutting head only.
The amplitude can also be changed during the sectioning pro-
cess.
Fig. 32
STAGE switch
Using this switch, you can move the specimen stage with the specimen
upwards or downwards quickly.
The movement continues as long as the switch is held in the respective
position. When the switch is released (mode switch must be in auto or
single position), it springs back into the center position and the move-
ment is interrupted.
Switch position:
Upwards --> Specimen stage moves upwards.
Downwards --> Specimen stage moves downwards.
Fig. 33
LED display
The LED display shows the values defined for section thickness in
single mode and the number of sections and section thickness in au-
tomatic mode.
• The LED (53, 54) in the THICKNESS (section thickness) switch or in
the SECTION (number of sections) switch is illuminated when the
corresponding parameter is being displayed (see Fig. 37).
Fig. 34
Leica VT1000 A
25
Download from Www.Somanuals.com. All Manuals Search And Download.
5.
Operation
The operating elements (continued)
The THICKNESS switch defines the desired section thickness for au-
THICKNESS switch
tomatic mode in microns.
• The parameter selected for this value is indicated in the LED dis-
play above the switch, the displayed values are specified in µm.
The LED (53) in the switch is then illuminated.
53
Switch position:
Upwards --> Values for the section thickness are increased.
Downwards --> Values for the section thickness are decreased.
Fig. 35
SECTION switch
Using the SECTION switch, the number of sections required for auto-
matic mode (AUTO) are defined.
• The value selected for this parameter is indicated in the LED display
54
above the switch; the LED (54) in the switch is then illuminated.
Switch position:
Upwards --> Number of sections is increased.
Downwards --> Number of sections is decreased.
• If this parameter is set to the value ‘000‘ manually, the instrument
switches to single sectioning mode until automatic mode is deacti-
vated via the switch.
Fig. 36
Additional control operations available in automatic mode
• After the automatic sectioning process starts, after each section,
the LED display shows the number of sections remaining, based on
the value defined for the number of sections.
• If both switches, THICKNESS and SECTION, are pushed upwards
or downwards simultaneously, the specimen stage is moved up or
down to the maximum or minimum height position. When this posi-
tion is reached, the LED (54) in the THICKNESS switch flashes.
Fig. 37
Operating Manual V 1.1 – 01/2010
26
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
The operating elements (continued)
The MODE switch serves to toggle between the manual, single and
MODE switch
automatic modes.
Switch position: MANUAL
• The instrument works in manual mode only; the LED display is not
illuminated. The section thickness must be adjusted using the sec-
tion thickness setting wheel (Fig. 3).
Switch position: SINGLE
• Only one section can be created at a time.
Fig. 38
• Using the THICKNESS switch, you can adjust the desired section
thickness; the value is shown in the LED display.
• The user must press up on the directional switch to start another
section. The instrument will retain the desired section thickness.
Switch position: AUTO
• Using this switch position, you can activate the automatic section-
ing mode of the instrument. In automatic sectioning mode, the oper-
ator can set the desired section thickness and number of sections.
The Vibratome carries out the task automatically. This is a great
benefit for serial sectioning.
• The desired section thickness can be configured using the THICK-
NESS switch and the number of sections can be configured using
the SECTION switch. The respective value appears in the display.
The automatic switch also serves as the reset button in auto-
matic mode. If you want to reset the instrument in serial sec-
tioning mode to the default parameters for sectioning opera-
tions and section thicknesses, the counters can be reset using
the automatic switch.
LIGHT switch
This simple switch switches on the fluorescent lamp and thus im-
proves the illumination of the specimen tray. Switch position:
ON --> Light is on.
OFF --> Light is off.
Fig. 39
Leica VT1000 A
27
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
The presentation angle of the sectioning blade
relative to the plane of the section is adjust-
able to suit operating conditions and specimen
types.
5.2 Adjustment options on the instrument
5.2.1 Adjusting the blade presentation
angle
The adjustment can be made by rotating the
blade holder (50) on the mounting bar.
• The accessories include a blade angle indi-
46
cator (46) for this purpose.
• To read the blade angle using this indicator,
hold the angle indicator against the left side
of the blade holder. The notch (47) must be
touching the mounting bar (49) of the blade
holder (50) and the lower edge must be
touching the specimen tray. (Fig. 40/41).
47
• For the adjustment, first unscrew the knurled
screw (48) on the blade holder with a screw
driver. (Fig. 41).
Fig. 40
Enlarged detail:
Scale of the blade angle
indicator and indicator on
the blade holder.
• During the adjustment, it is important that
the bottom edge of the blade angle indica-
tor is touching the main specimen bath. (23)
(arrow in Fig. 41). For this purpose, it may be
necessary to move the cutting head forward.
51
• Now, rotate the blade holder until the small
indicator (51) specifies the desired value on
the scale of the blade angle indicator.
48
• Retighten the knurled screw so that it cannot
49
come loose during sectioning.
50
• For additional information about adjusting
the blade holder, refer to the following sec-
tion.
23
Fig. 41
Operating Manual V 1.1 – 01/2010
28
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
Positioning the specimen in the specimen
holder
5.2.2 Adjusting the specimen and blade
You can adjust the lateral position of the speci-
men in order to attain an approximate centering
of the specimen in the specimen tray. Since one
of the jaws of the specimen holder is stationary,
the use of specimen mounting blocks of vary-
ing widths will allow for adjustable specimen
mounting.
Fig. 42
Adjust the tilt of the specimen holder.
You can adjust the tilt of the specimen holder in
one axis in order to make the specimen surface
approximately horizontal.
• To do so, first unscrew the locking screw
with a plastic head between the front side of
the holder and the wall of the specimen tray.
• You can now tilt the entire specimen holder
to one side.
Fig. 43
Leica VT1000 A
29
Download from Www.Somanuals.com. All Manuals Search And Download.
5.
Operation
Adjusting the tilt of the specimen
If the specimen is still not completely horizon-
tal, you can tilt the specimen along another axis
(from side to side).
To do so, tilt the specimen mounting block
slightly when it is clamped in the jaws of the
specimen holder.
Complete control of the specimen over
all three axes is possible with the ro-
tating stage assembly (see Chap. 7.2,
Optional Accessories).
Fig. 44
Moving the blade holder relative to the specimen
In some cases, e.g. for large specimens, it is
necessary to move the blade holder (50) on the
mounting bar.
49
48
When the width of a large specimen is approxi-
mately the same as the sectioning blade edge
length, the lateral positioning of the sectioning
blade may have to be adjusted to ensure com-
plete sections.
50
Fig. 45
• By loosening the knurled screw (48), you can
move the blade holder sideways along its
axis (49) and thus position it more accurately
relative to the specimen (Fig. 45).
Because the knurled screw (48) is also
used to adjust the blade presentation
angle, the angle may have changed.
Therefore, recheck the blade angle af-
ter this adjustment.
Operating Manual V 1.1 – 01/2010
30
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
5.2.3 Adjustable sectioning window
The VT1000A features an adjustable sectioning window that you can use
you speed up the process of making series sections.
Fig. 46
This "window" is the distance the blade travels
If the front and rear edge of the "window" are
positioned as close as possible to the front and
rear end of the specimen, it is only necessary to
cover distance "B" for a section.
from a resting retracted position to the front of
the bath when it automatically ends the forward
stroke and reverses.
This corresponds to distance "A" in Fig. 46.
This reduces the time for a complete cycle or
section significantly.
Leica VT1000 A
31
Download from Www.Somanuals.com. All Manuals Search And Download.
5.
Operation
Adjustable sectioning window (continued)
To make this adjustment, the instrument should
be in manual mode.
To adjust the front and rear edge of the section-
ing window, you have to move the internal limit
switch using two rotary knobs (57), which are
on the side of the top plastic cover of the Vi-
bratome. The two rotary knobs can be adjusted
independently of one another.
57.2
57.1
Fig. 47
• First, adjust the front limit switch that is on
• To adjust the rear limit switch, turn the knob
(57.2) counterclockwise to disengage the
switch.
the front side of the instrument.
• To do so, release the knob (57.1) with the
blade fully retracted and move it into the de-
sired position.
• Then, start the sectioning operation using
the switch for the feed movement of the Vi-
bratome.
• As soon as the switch is in the desired posi-
tion, tighten the knob again so that it cannot
be disengaged during sectioning.
• During the feed movement of the blade,
push the switch forward until it reaches the
desired position.
• The rear switch can be moved only while the
instrument is in operation.
• Finally, tighten the knob to fix the switch in
place.
• You can readjust the sectioning window
whenever new specimen sizes are used.
Operating Manual V 1.1 – 01/2010
32
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
5.2.4 Adjusting the specimen height
The thickness of sections generated is controlled
by the raising of the specimen incrementally.
Since the sectioning blade remains stationary
along the vertical axis, the increment by which
the specimen is raised correlates to the section
thickness.
The height of the specimen is adjusted using a
micrometer feed system that is located below
the specimen tray.
27
The rotational movement of the setting wheel (58)
for the section thickness is converted into a ver-
tical movement by the micrometer feed system.
Turning the wheel clockwise raises the specimen
stage (27) on which the specimen holder is fas-
tened, while turning it counterclockwise lowers it
.
When turned, you can feel the setting wheel en-
gage in notch increments of 5 µm.
58
59
60
The scale (59) is divided into µm increments
(10-6 meter), where the scale values are se-
lected randomly, i.e. it is not important where
the black pointer (60) of the scaled adjusting
knob is. To create a section with a thickness of
60 µm, the setting must be increased by 60 scale
increments.
Fig. 48
Important!
The respective setting is always consistent in one direction of rotation only.
Example:
If the setting wheel has been turned clockwise to the setting "30", then turned back counter-
clockwise to "25" immediately afterwards, this does not lower the specimen by 5 µm. To actu-
ally lower the specimen by 5 µm, you have to turn the wheel approx. one quarter turn counter-
clockwise PAST the setting "25", then back to "25" again.
This means that to compensate for the small amount of play in the micrometer feed system, be-
fore each adjustment in the direction opposite to the last one, you first have to turn the setting
wheel one-quarter turn past the new value. Only then can you make the desired adjustment.
Leica VT1000 A
33
Download from Www.Somanuals.com. All Manuals Search And Download.
5.
Operation
5.3 Sectioning
The Leica VT1000 A offers a wide variety of options for sectioning fixed and unfixed tissue
samples of plant or animal origin. It employs a vibrating blade principle, which allows sec-
tioning without freezing or embedding. This makes it possible to prevent the unwanted effects
that occur during freezing or embedding, such as the formation of artifacts, change of the
morphology, the impairment of enzyme activities and other harmful processes.
The patented vibrating blade principle moves the sectioning blade edge in a reciprocating
arcuate path as it penetrates the specimen. The reduced effective edge angle from the trans-
verse movements, together with the lateral distribution of the cutting edge penetrating pres-
sure, minimize elastic deformation of soft tissue specimens that are simply held or encapsu-
lated in position during the sectioning operation. Uniform sections, as such, can be made of
delicate soft tissue specimens. Sections made are free of observable compressive distortion
in the direction of cut, as would be typical with conventional microtomes. Because the tissue
cells on the surface of the sections are not damaged, the ultrastructure in the sections is also
kept intact.
The sectioning process takes place in a specimen bath. The liquid serves as a lubricant for
the blade; it also keeps the specimen temperature constant and maintains or reinforces the
desired features of the specimens. It also serves to facilitate the easy retrieval of the sec-
tions from the bath.
The instrument works semi-automatically; this means that the operator only has to initiate
the sectioning operation. After finishing a section, the instrument remains in standby mode
until the section is removed or another sectioning operation is started. The amplitude of the
blade vibration as well as the feed rate and presentation angle of the blade can be adjusted
individually by the operator, making it possible to attain optimum results for a wide variety of
specimens.
There are essentially three operating states for the Electronics Assembly:
A. Sectioning blade advancing FORWARD with vibratory movement ON.
B. RETRACTION of the blade with vibratory movement OFF.
C. Sectioning blade in a rearmost "READY" position with vibratory movement OFF.
Operating Manual V 1.1 – 01/2010
34
Download from Www.Somanuals.com. All Manuals Search And Download.
Sectioning (continued)
When the instrument is switched on for the first time, the blade is automatically moved back
in operating state "B" and stopped in state "C".
Each sectioning cycle needs only to be initiated by the operator. Once state "A" is activated,
the sectioning blade will advance at the operator-selected forward speed and amplitude
until it reaches the forward end-of-travel. The blade then switches into operating state "B"
automatically until state "C" is reached. It will then remain in state "C" until another cycle is
activated by the operator.
When in state "A", a momentary override of the preselected forward speed is available.
In this way, you can select the maximum feed rate for the movement between the rearmost
"ready" position and the specimen.
The retraction movement of the blade in operating state "B" always takes place at the maxi-
mum speed, regardless of the preset feed rate.
At any time during the sectioning cycle, operator override of the semi-automatic operation is
available. Thus during feed in operating state "A", the blade can be moved back in state "B"
and vice versa.
Leica VT1000 A
35
Download from Www.Somanuals.com. All Manuals Search And Download.
5.
Operation
5.3.1 General instructions
As a result of the great variety of specimen types, sizes, shapes, states and preparations,
etc. that can be encountered while sectioning with the VT1000 A, it is not possible to provide
specific recommendations for parameter settings. The optimal settings for the individual ap-
plications can be determined only empirically using specimens that are no longer needed and
are as similar as possible to the actual specimens to be sectioned.
The most important function of the instrument is producing tissue sections without prior
freezing or embedding. This is made possible by a lateral movement of the blade while it pen-
etrates the specimen. Therefore, the ratio between the lateral speed (which is proportional
to the amplitude adjustment) and the feed rate is one of the central parameters for attaining
high-quality sections for the widest variety of specimen types.
Generally, solid specimens can be sectioned
with a small ratio of amplitude to feed rate (i.e. a
high feed rate).
For soft specimens, on the other hand, we rec-
ommend a large ratio of amplitude to feed rate
(i.e. a large amplitude).
If the section breaks up due to excessive agita-
tion, the amplitude setting should be reduced.
In normal sectioning, the specimen is lifted up-
wards elastically as the blade advances. This is
illustrated in Fig. 49.
Fig. 49
A small clearance angle to the specimen will exist when the blade reverses after a section.
This lifting phenomenon varies with parameter settings and is more pronounced with softer
specimens, higher advance speeds, higher blade presentation angles, and particularly with
thicker sections being generated.
In general, it does not materially affect the section performance provided that parameter set-
tings are not changed during a section and gradual transitions are made in section thickness
changes (especially going from thick sections to thin sections) during serial sectioning.
Operating Manual V 1.1 – 01/2010
36
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
Direct mounting
5.3.2 Inserting the specimens
Specimens rigid enough to be held firmly in
the specimen holder without damage may be
clamped directly.
Keep in mind that excessive clamping forces on
the jaws of the specimen holder can cause ten-
sion in the specimen.
Softer specimens that do not have enough
strength on their own (such as a leaf) can be
placed between carrier strips or two layers of
soft material, e.g. balsa wood or low melt aga-
rose, then inserted into the specimen holder.
The supporting material should cut easily and
may be separated from the tissue section in the
bath area after sectioning.
To ensure sufficient strength, the specimen
should, where possible, be clamped in the hold-
ing jaws directly or with the least amount of in-
lay material possible.
Fig. 50
Adhesive mounting
Fragile specimens that are difficult to align correctly can be adhered directly or indirectly to
the specimen mounting blocks, which are included in the accessories.
Use only the cyanoacrylate adhesive provided for adhering the specimens. Using this adhe-
sive, the widest variety of materials (e.g. unfixed liver tissue, heart tissue, fixed brain and kid-
ney tissue or fragile plant specimens) can be fixed on a specimen holder quickly and reliably.
For specimens that are insufficiently rigid in unfixed condition for adhesive mounting, prior
fixation may be performed if not detrimental to the phenomenon or process for which speci-
mens are being studied.
Alternately, the specimens may be encapsulated in a support medium such as agar or gela-
tin. The block of support medium, together with the specimen, may be trimmed to size and
adhesive mounted upon solidification.
Leica VT1000 A
37
Download from Www.Somanuals.com. All Manuals Search And Download.
5.
Operation
Adhesive Mounting (continued)
Note that in all cases requiring adhesive mounting, thickness of the specimen should be kept
as small as practical to maximize rigidity.
Before sectioning with the VT1000 A, create a uniform, level surface along the cutting edge of
the tissue; this will reduce the time needed for cutting the specimen to size using the blade.
Be very careful when using the adhesive, as it very easily sticks to human skin. Avoid
direct skin contact with the adhesive under any circumstances.
If, despite this, you come into direct contact with the cyanoacrylate adhesive provided,
first allow the adhesive to air dry, then wipe it off with a towel soaked in acetone. When
unintentional bonding of skin occurs, separate by a "peeling" (rather than pulling) action
after applying acetone to bond area.
Instructions for a clean bond
The surfaces onto which the specimens are attached must be clean, dry and free of adhesive
residue from previous applications.
The specimen mounting blocks specimen tray, and deluxe specimen bath have a hard sur-
face coating that should not be impaired, even by repeated sectioning operations with razor
blades.
• Carefully wipe the surface of the specimens to be attached using absorbent paper to
remove fluid residue that could impair the adhesion.
• Apply a sufficient quantity of adhesive to the contact surface so that the entire specimen
is fixed.
• Experience has shown that most deviations in the quality of the sections is due to partial
detachment of the specimens.
• Because the adhesive effect sets in very quickly, the specimen should be applied to the
adhesive surface carefully and accurately the first time.
• If the specimen permits, carefully press on it to increase the adhesive effect.
Operating Manual V 1.1 – 01/2010
38
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
Adhesive Mounting (continued)
Specimens small enough to fit on a surface
of a specimen mounting block can be affixed
directly.
The specimen mounting block is then clamped
into the jaws of the specimen holder for
sectioning.
If the specimen requires a larger surface, you
can use specimen tray (39053744) or the deluxe
specimen bath (39053745).
Fig. 51
For specimens that are insufficiently rigid in unfixed condition for adhesive mounting, prior
fixation may be performed if not detrimental to the phenomenon or process for which speci-
mens are being studied. Alternately, the specimens may be encapsulated in a support medi-
um such as agar, gelatin, or paraffin. The block of support medium, together with the speci-
men, may be trimmed to size and adhesive mounted upon solidification.
Note that in all cases requiring adhesive mounting, thickness of the specimen should be
kept as small as practical to maximize rigidity. Create a uniform, level surface with a cursory
manual processing; this will reduce the time needed for cutting the specimen to size using
the blade.
5.3.3 Selecting and inserting the blade
Depending on the type of specimens sectioned, the type and brand of blades may affect
the instrument performance.
Before either type of blade is used for sections, it must be cleansed thoroughly of oil
or silicone residue. To do so, we recommend placing the blade in a xylene bath for
approximately 10 minutes, then flushing it with acetone and allowing it to air dry.
Leica VT1000 A
39
Download from Www.Somanuals.com. All Manuals Search And Download.
5.
Operation
Inserting the blade
Be careful when handling the dispos-
able blades or sapphire knife.
The cutting edge is extremely sharp
and can cause serious injuries.
55
Before inserting a blade, the specimen
to be section should be attached to the
specimen plate (clamped into place or
affixed) to prevent injuries.
53
54
Fig. 52
The blade is held in place by a spring clip (53) in
the blade holder that presses against the bot-
tom of the blade holder.
55
• First, push the spring clip (53) upwards using
your left thumb (Fig. 52).
• To insert the blade, hold it (54) by its dull
ends using your thumb and index finger and
insert it into the blade holder (55) (Fig. 43),
then carefully release the spring clip - Fig. 44
shows the correctly inserted blade.
Fig. 53
For high-quality sections, a sapphire knife is available (39 053237). This knife fits in the
standard blade holder and provides a superior means for sectioning tissue.
When handling the blades, exercise particular caution to ensure that the blade edge
does not contact any object. Resulting microscopic damage to the blade edge may
cause localized tearing of the specimen. Caution must also be exercised when han-
dling the blades to prevent operator injury from accidental cuts.
Operating Manual V 1.1 – 01/2010
40
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
5.4 Specimen preparation
Prior to sectioning, gross trimming of the specimen top surface with the sectioning blade to
achieve flatness is required.
After the specimen and mounting block have
been clamped into the specimen holder in the
desired orientation relative to the blade feed
direction, the top surface of the specimen
should be kept approximately horizontal.
Two means of adjustment are available: the tilt
of the specimen holder and the tilt of the speci-
men mounting block within the specimen holder
jaws.
Fig. 54
The speed and amplitude should be set to "0" initially. Then, advance the blade until it almost
touches the specimen.
For this purpose, briefly push the direction switch upwards. With the sectioning blade close
to the specimen, their relative heights may be gauged approximately. The specimen should
then be raised (or lowered) to a position just below the blade edge.
The specimen may then be trimmed by serial sectioning until complete sections can be made
of the area of interest on the specimen. With unfamiliar specimens, to minimize the chance
of specimen damage, the advance speed should be at a low setting while the amplitude
should be at a medium setting. The section thickness should be increased at suggested in-
tervals of 50 µm.
If the specimen permits, the speed and section thickness may be gradually increased to re-
duce trimming time. However, be careful so that the specimen does not twist or fall out of the
specimen holder.
Leica VT1000 A
41
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
5.5 Preparing the specimen bath
The purpose of the specimen bath is four-fold: to lubricate the blade dur-
ing the sectioning operation, to prevent the specimen from heating up or
drying out, to increase or maintain desired features of the specimen and
to allow effortless removal of the fragile sections.
Any fluid, compatible with polyproylene and nitrile rubber, that would best
preserve or maintain the phenomenon or process for which the specimen
is being studied may be used. A wide variety of fluids have been used
successfully ranging from a balanced saline solution, buffered phosphate
solutions, alcohols, hydrocarbon, distilled or deionized water, glycerols,
mineral oils and formalin.
The fluid chosen should not cause the rapid swelling of the specimen. In
case such a fluid must be used, the specimen should be allowed to swell
in the fluid before being mounted in the specimen holder. Normal saline
solution is recommended for unfixed animal tissue. Distilled or deion-
ized water may be used for fixed animal tissues and botanical specimens.
Caution must be exercised when using toxic or inflammable fluids, as they
may pose a risk of injury to the operator.
Any bath fluid may be externally cooled (or heated) to achieve maximum
sectioning consistency or to preserve enzymatic activity. In most appli-
cations, the specimen bath is maintained at just above freezing using the
immersion probe of a separate cooling unit. For information on possible
sources of supply, refer to the section entitled "Accessories". Alternately,
if the specimen bath is aqueous, ice can be added.
After setting the desired blade presentation angle, fill the bath until the
blade edge is about 3 to 4 mm below the surface. At this fill level, the
section will either lie directly on the bent front of the blade holder or float
directly in front of the blade.
Operating Manual V 1.1 – 01/2010
42
Download from Www.Somanuals.com. All Manuals Search And Download.
5. Operation
5.6 Conventional sectioning
After the specimen has been trimmed to create a flat specimen surface,
the preparations for actual sectioning can be carried out. The procedure
consists of generating a few sections at the final parameter settings and
section thickness. This is intended to compensate for the effect of lifting
the specimen described in the section entitled "General instructions". This
effect can be observed in that the first, and possibly the next few, sections
will be either nonexistent or too thin. Later, when changing from thick
sections to much thinner sections, the procedure should be repeated.
As soon as the optimal parameter settings have been determined accord-
ing to the procedure recommended in the "General instructions" section,
the sectioning operation can be carried out with these settings. Note that
while a section is being generated, the parameter settings must not be
varied, otherwise the thickness may also vary within the section. Note
also that the section thickness may be increased (by turning in the clock-
wise direction) only after the sectioning blade has passed the specimen
while reversing. If the rotary knob for section thickness is turned counter-
clockwise, a subsequent compensation for the inner play is required (see
the section on "Adjusting the height of the specimens").
Because the instrument operation is semi-automatic, the operator only
has to initiate each sectioning cycle. The instrument will then complete
the section, unattended if desired, and return to the rearmost "ready"
position. With an increase in the speed setting, the blade can be moved
quickly from the "ready" position towards the specimen. In this case, a
sufficient "braking distance" should be provided to slow down the blade to
the preset feed rate before it touches the specimen.
The lamp/magnifier assembly may be used to facilitate close observation
of the specimen during sectioning.
Leica VT1000 A
43
Download from Www.Somanuals.com. All Manuals Search And Download.
5.
Operation
5.7 Removing the sections
Sections generated may be retrieved from the specimen bath by various means.
For microscopic applications, the section may be delivered to a glass slide by aspirating the
section with a small glass dropper if doing so does not damage the specimen.
In some cases, using a fine sable brush provides more satisfactory results, since the risk of
section damage is reduced.
In other cases, the section can be transferred to another specimen bath and picked up there
using a partially submerged glass specimen slide.
The sable brush or a blunt glass rod may be used to manipulate the section while it is in the
specimen bath.
Once the section is positioned properly on the glass specimen slide, it may be adhered with
albumin or other mounting media.
Staining or other treatment, then coverslipping, may be carried out as for a typical conven-
tional section for microscopy.
After you have obtained all the sections you need, the entire area of the specimen tray should
be cleaned (refer to Chapter 6, "Cleaning").
Operating Manual V 1.1 – 01/2010
44
Download from Www.Somanuals.com. All Manuals Search And Download.
6. Cleaning and Maintenance
6.1 Cleaning the instrument
• Always remove the blade before detaching the blade holder from
the instrument!
• Always keep the sapphire blades in the box when not in use!
• Never place used blades on the laboratory table - dispose of them
safely!
• When using detergents, comply with the safety instructions of the
manufacturer and observe the laboratory regulations valid in the
country of use.
• When cleaning the outer surfaces, do not use xylene, scouring
powders or solvents containing acetone or xylene. The finished
surfaces are not resistant to xylene or acetone!
• Ensure that no liquids enter the interior of the instrument when
cleaning!
Before each cleaning, carry out the following preparatory steps:
• Move the specimen vice assembly into the lower end position.
• Remove the blade from the blade holder and dispose of it safely (be-
fore disposing of used blades, apply thick "Scotch" tape to the cutting
edge or wrap the entire blade in paper) or place the sapphire blade in
the knife case.
• Remove the specimen from the specimen vice assembly.
• Remove section waste and dispose of it safely.
• Remove specimen vice assembly and clean separately.
• Do not open any covers or casing panels of the instrument, as
electrically live parts are on the inside!
Operating Manual V 1.1 – 01/2010
45
Download from Www.Somanuals.com. All Manuals Search And Download.
6.
Cleaning and Maintenance
Specimen bath area
After sectioning is completed, the specimen bath, specimen holder and
sectioning blade holder should be cleansed of any bath solution residue.
Any water-soluble solvent may be used unless it corrodes parts made
of polypropylene or nitrile rubber. The final flushes should be with clean
water.
This procedure is intended to premature corrosion of metal components
in this area, buildup of substances or contamination of subsequent baths.
Magnifier lens
The lens in the lamp and magnifier module should be cleaned if possible
using a soft cloth soaked in ethanol or conventional glass cleaner. A final
buffing with clean dry tissue should follow.
Cleaning the instrument and outer surfaces
Any liquids spilled on the instrument should be wiped off immediately.
• If necessary, the varnished outside surfaces can be cleaned with
a mild (nonabrasive) commercial household cleaner or soapy water
and then be wiped with a moist cloth.
• The instrument must be completely dry before it can be used again.
• Anodized parts (e.g. the specimen clamps) can also be cleaned with
solvents.
A vinyl instrument cover is provided in the accessory package for protec-
tion against dust or scratching between use.
Operating Manual V 1.1 – 01/2010
46
Download from Www.Somanuals.com. All Manuals Search And Download.
6. Cleaning and Maintenance
6.2 Maintenance instructions
Only authorized, qualified Leica service personnel may access the
internal components of the instrument for service or repair!
When used normally, the Vibratome VT1000 A requires only standard
maintenance.
To ensure trouble-free operation of the instrument over a long period of
time, the following is recommended by Leica:
• Thoroughly clean the instrument on a daily basis.
• From time to time, oil the specimen holder (see Fig. 4, Page 9), blade
holder and specimen clamp (e.g. after cleaning in the sterilization oven
or using solvents).
• Have the instrument inspected according to the maintenance contract
by a qualified service engineer authorized by Leica.
The intervals depend on how heavily the instrument is used.
All service repairs covered under the Warranty Policy shall be
performed at no expense to the user.
In case of a complete failure of the instrument, follow this procedure:
• First check the power supply on the power socket.
• Then, check the fuse(s) on the rear side of the instrument - see
Chapter 6.3 "Replacing components". For this purpose, it is mandatory
to disconnect the power cable from the power supply.
• If a fuse is defective, you must have identified and corrected the cause
of the burned-out fuse before plugging the power cable back in. If no
fuse is defective, please contact Leica service.
Leica VT1000 A
47
Download from Www.Somanuals.com. All Manuals Search And Download.
6.
Cleaning and Maintenance
6.3 Replacing components
6.3.1 Fuse
If the instrument fails to function completely, first check for power avail-
ability at the power outlet. The instrument fuse(s) located at the rear of
the instrument should be checked next. Before removing the fuse, pull the
POWER PLUG OUT OF THE SOCKET. The fuse and its holder are removed
by lifting the tab in the fuse drawer that is located directly above the plug
and pulling the drawer out of the socket.
Check to see if the filament within the glass tube of the fuse is intact. If
not, replace with fuse referenced below, two of which are supplied in
the accessory package. Before reconnecting the power cord, check the
instrument for any obvious cause of the burnt fuse and make the appropri-
ate correction.
Model #
Qty
1
Fuse
VT1000 A,
110V
39GMA-2A
VT1000 A,
220V
2
39F-1A
6.3.2 LED light
If the LED light fails to turn on or flickers when operating, replacement is
necessary. The LED light is located under the lamp assembly housing, di-
rectly behind the glass magnifier.
BE ABSOLUTELY CERTAIN TO UNPLUG THE POWER CABLE before
removing the lamp.
The lamp can be removed from its mounting sockets by first removing the
end cap of the lamp housing opposite to the socket. Once the socket is
removed, the lamp tube can be removed by pulling the lamp straight out of
the socket.
Operating Manual V 1.1 – 01/2010
48
Download from Www.Somanuals.com. All Manuals Search And Download.
7. Optional Accessories
7.1 Ordering information
Specimen mounting block, 3 per package.......................................... 39053747
Specimen adhesive................................................................................. 39053986
LED light .................................................................................................... 39053226
Feather Blades, 10 per package ........................................................... 39053234
Specimen Tray......................................................................................... 39053744
V-block adapter ....................................................................................... 39053750
Specimen holder, 1/2 head .................................................................... 39053753
Rotary table module (ball and socket) ................................................. 39053760
Replaceable rotating mounts ................................................................ 39053763
Sapphire knife.......................................................................................... 39053237
Specimen Retrievers............................................................................... 39053220
Section Mounting Device....................................................................... 39053225
Foot Pedal ..............................................................................................39053300A
Leica VT1000 A
49
Download from Www.Somanuals.com. All Manuals Search And Download.
|