5185-5991 5 KDa MWCO 4 mL
5188-5201 30 KDa MWCO 4 mL
5188-5202 50 KDa MWCO 4 mL
5188-2798 5 KDa MWCO 15 mL
5188-2799 30 KDa MWCO 15 mL
5188-2800 50 KDa MWCO 15 mL
Agilent Spin Concentrators for Proteins
Instructions – 4 mL Concentrators
Equipment required
Centrifuge
4 mL Spin Concentrators, pack of 25
Rotor type
Swing bucket or Fixed angle
Minimum rotor angle
Rotor cavity
25°
To fit 15 mL (17 mm) conical bottom tubes
Minimum effective speed
Recommended speed
Maximum speed
Concentrate recovery
Pipette type
Recommended tip
Technical specifications
Concentrator sample volume capacity
Swing bucket rotor
Fixed angle rotor
Dimensions
2,000 g
5,000 g
7,500 g
Fixed or variable volume
Thin gel loader type
1-4 mL
1-4 mL
Total Length
Width
Active Membrane Area
Hold-up volume of membrane
Dead stop volume
pH range
122 mm
17 mm
2.0 cm2
<10 µL
20 µL
5,000 Molecular Weight Cut-Off (MWCO)
4 mL Spin Concentrator, Part No. 5185-5991
pH 1 – 9
Materials of construction
Body
Filtrate vessel
Concentrator cap
Membrane
Polycarbonate
Polypropylene
Polycarbonate
Polyethersulfone
Concentrating Proteins with 4 mL Concentrators:
Desalting/Buffer Exchange
1
2
3
Fill concentrator with sample within the working volume
capacity range noted in the table above (ensure that the hinged
lid is fully seated).
1
Concentrate sample to desired level or at least
5P.
2
3
4
Empty filtrate container.
Insert assembled concentrator into centrifuge (when fixed
angle rotors are used, angle the concentrators so that the
printed windows face upwards/outwards).
Centrifuge at recommended speeds, taking care not to exceed
the maximum g force indicated in the table above (20 minutes
at 7,500 g is recommended for 30P concentration of 4 mL at
10LC).
Refill concentrator with an appropriate buffer.
Concentrate the sample again and repeat the
process until the concentration of the
contaminating species is sufficiently reduced.
Typically three wash cycles will remove 99% of
initial salt content.
4
Once the desired concentration is achieved, remove assembly
and recover sample from the bottom of the concentrate pocket
with a pipette.
See Notes on next page.
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