Leica Microscope Magnifier DM500 B User Manual

Leica DM500, DM500 B  
Manual  
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Safety regulations  
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Manual  
Leica DM 500, DM500 B  
4
 
Safety concept  
The individual modules of the Leica DM micros-  
copy series include an interactive CD-ROM with  
all relevant user manuals several languages.  
Keep it in a safe place, and readily accessible  
to the user. User manuals and updates are also  
available for you to download and print from  
our web site at  
You can combine individual system articles with  
articles from external suppliers. Please read the  
user manual and the safety requirements of the  
supplier.  
Before installing, operating or using the instru-  
ments, read the user manuals listed above. In  
particular, please observe all safety instruc-  
tions.  
This operating manual describes the special  
functions of the individual modules of the Leica  
DM microscopy series and contains important  
instructions for their operational safety, main-  
tenance, and accessories.  
To maintain the unit in its original condition  
and to ensure safe operation, the user must  
follow the instructions and warnings contained  
in these user manuals.  
The "Safety concept" booklet contains addi-  
tional safety information regarding the service  
work, requirements and the handling of micro-  
scope, accessories and electrical accessories as  
well as general safety instructions.  
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Manual  
Leica DM 500, DM500 B  
5
 
Symbols used in this operating manual  
Danger due to hot surface.  
Warning of a danger  
This symbol indicates especially impor-  
tant information that must be read and  
This symbol warns against touching  
accessible hot surfaces, e.g. those of  
light bulbs.  
complied with.  
Failure to comply can cause the following:  
For Model DM500 B (Battery Version)  
Dispose of old batteries and defective  
appliances in accordance with the stat-  
utory regulations.  
Ϙ
Hazards to personnel  
Ϙ
Functional disturbances or damaged instru-  
ments  
Warning of hazardous electrical voltage  
This symbol indicates especially impor-  
tant information that is mandatory to  
read and observe.  
Important information  
This symbol indicates additional infor-  
mation or explanations that intend to  
provide clarity.  
Failure to comply can cause the following:  
Ϙ
Hazards to personnel  
Explanatory notes  
ϐ
This symbol within the text points to addi-  
tional information and explanations.  
Ϙ
Functional disturbances or damaged  
instruments  
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Manual  
Leica DM 500, DM500 B  
6
 
Important Notes  
Description  
User Manual  
Accessories from third-party suppliers  
You can combine individual system articles with  
articles from external suppliers. Please read the  
user manual and the safety requirements of the  
supplier.  
The Leica DM500 and DM500 B microscope meets  
today's state of the art of technology. Neverthe-  
less, hazards may still arise during operation.  
The potential risks are described below.  
This user manual includes important instruc-  
tions related to operating safety, maintenance  
and accessories,  
Your Leica DM500 or DM500 B micro-  
scope comes with an interactive CD-ROM  
with all relevant user manuals. Keep it in a safe  
place, and readily accessible to the user. User  
manuals and updates are also available for you  
to download and print from our web site at  
Before installing, operating or using the  
instruments, it is required to read this  
user manual. In particular, please observe all  
safety instructions.  
Original condition  
To maintain the unit in its original condition  
and to ensure safe operation, the user must  
follow the instructions and warnings contained  
in these user manuals.  
Legal requirements  
Contact address  
Adhere to general and local regulations relat-  
ing to accident prevention and environmental  
protection.  
Leica Microsystems (Switzerland) Ltd.  
Industry Division  
Max Schmidheiny Strasse 201  
CH-9435 Heerbrugg (Switzerland)  
EC Declaration of Conformity  
Electricallyoperatedaccessoriesareconstructed  
based on the state of the art of technology and  
are provided with an EC Declaration of Confor-  
mity.  
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Leica DM 500, DM500 B  
7
 
Instructions on use  
Intended use  
Place of use  
Non-intended use  
Using the instrument in any way  
contrary to the specifications in the user  
manual can lead to bodily harm and damage to  
objects. Doing so can impair the functions of  
the protective measures provided.  
The Leica DM500 and DM500 B microscopes are  
optical instrument which use magnification and  
illumination to improve the visibility of objects.  
It is used for observing and documenting.  
Only use the instruments in closed, dust free  
rooms and between +10 °C and +40 °C. Protect  
the devices from oil, chemicals and extreme  
humidity. If using the devices outdoors, protect  
them from dust and moisture. Never use electri-  
cal devices outdoors. Install electrical devices at  
least 10 cm from the wall and away from flam-  
mable substances.  
The Leica DM500 and DM500 B microscopes  
may be used only in closed rooms and must be  
placed on a solid floor.  
Never install any other plug (NEMA  
5-15P) or unscrew any mechanical  
components unless expressly instructed to do  
so in the instructions.  
The Leica DM500 or DM500 B microscopes can  
be used in clean rooms without any problems.  
Avoid large temperature fluctuations, direct  
sunlight and vibrations. These conditions  
can distort measurements and micrographic  
images.  
CAUTION: Risk of explosion if battery is  
replaced by an incorrect type. Dispose  
of used batteries according to the instructions.  
Position the Leica DM500 or  
DM500 B microscope so that it can be  
disconnected from the power supply at any  
time. The power cord must remain accessible at  
all times, because the power supply is foreseen  
as mains disconnecting device.  
In warm and warm-damp climatic zones,  
the individual components require  
special care in order to prevent the build-up of  
fungus.  
The devices and accessories described in  
this operating manual have been tested  
for safety and potential hazards.  
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Leica DM 500, DM500 B  
8
 
Instructions on use (continued)  
The responsible Leica affiliate must be  
consulted whenever the instrument is  
altered, modified or used in conjunction with  
non-Leica components that are outside of the  
scope of this manual.  
Transport  
Integration in third-party products  
When installing Leica products into third-party  
products, the manufacturer of the complete  
system or its dealer is responsible for follow-  
ing all applicable safety instructions, laws and  
guidelines.  
If at all possible, use the original packaging for  
shipping or transporting individual modules.  
In order to prevent damage from vibrations,  
disassemble all moving parts that (according to  
the user manual) can be assembled and disas-  
sembled by the customer and pack them sepa-  
rately.  
Unauthorized alterations to the device  
or noncompliant use shall void all rights  
to any warranty claims.  
Disposal  
Disposal must comply with locally applicable  
laws and regulations.  
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Leica DM 500, DM500 B  
9
Health risks and dangers of use  
Health risks  
The ergonomic design and construction of the  
Leica microscopy series are intended to reduce  
the exertion of the user to a minimum.  
Dangers during use  
Workplaces with microscopes facilitate  
and improve the viewing task, but they  
also impose high demands on the eyes and  
holding muscles of the user. Depending on the  
duration of uninterrupted work, asthenopia  
and musculoskeletal problems may occur. For  
this reason, appropriate measures for reduction  
of the workload must be taken:  
Ϙ
The Leica DM500 or DM500 B microscope  
may only be connected to a grounded  
socket.  
Danger of infection  
Direct contact with eyepieces is  
potential transmission method for viral  
infections of the eye.  
a
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The Leica DM500 or DM500 B microscope  
may not be operated unless it is in proper  
functioning condition.  
The risk can be kept to a minimum by  
using personal eyepieces for each indi-  
vidual or detachable eyecups.  
LED Klasse 1 /Class 1 LED Product  
The illumination meets laser class 1 require-  
ments in accordance with IEC 60825-1 "Safety  
of LED products".  
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Ϙ
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Optimum workplace layout  
Frequent changes of activity  
Thorough training of the personnel, giving  
consideration to ergonomic and organiza-  
tional aspects  
Caution: Use of controls or adjustments or  
performance of procedures other than those  
specified herein may result in hazardous radia-  
tion exposure!  
Class 1  
LED class 1  
LED Product  
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Leica DM 500, DM500 B  
10  
 
Information for the person responsible for the instrument  
Information for the person responsible for  
the instrument  
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Ϙ
Only original Leica replacement parts may  
be used in servicing the product.  
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Ϙ
Do not use the Leica DM500 or DM500 B  
microscope unless it is in perfect condition.  
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Ϙ
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Ensure that the Leica DM500 or DM500 B  
microscope is used only by qualified person-  
nel.  
After service work or technical modifica-  
tions, the unit must be reconfigured with  
observance to our technical requirements.  
Inform your Leica representative or Leica  
Microsystems (Schweiz) AG, Industry Divi-  
sion, 9435 Heerbrugg, Switzerland, imme-  
diately of any product defect that could  
potentially cause injury or harm.  
Ensure that this user manual is always avail-  
able at the Leica DM500 or DM500 B micro-  
scope.  
Ϙ
If the unit is modified or serviced by unau-  
thorized persons, is improperly maintained  
(as long as maintenance was not carried  
out by us), or is handled improperly, Leica  
will not accept any liability.  
Carry out regular inspections to make  
certain that the authorized users are adher-  
ing to safety requirements.  
Ϙ
If you use accessories made by third-party  
manufacturers with the Leica DM500 or  
DM500 B microscope, be sure that each  
such manufacturer confirms that the  
combination is safe to use and observe the  
product's user manual.  
Ϙ
The electric installation in the building  
must conform to the national standard, e.g.  
current-operated ground leakage protec-  
tion (fault-current protection) is suggested.  
When instructing new users, do so thor-  
oughly and explain the meanings of the  
warning signs and messages.  
Ϙ
Modifications and maintenance of the Leica  
DM500 or DM500 B microscope may only  
be performed by professionals expressly  
authorized by Leica.  
Assign individual responsibilities for start-  
ing, operating and servicing the instru-  
ment and monitor the observance of these  
responsibilities.  
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Leica DM 500, DM500 B  
11  
 
Care instructions  
General instructions  
Cleaning coated parts and plastic parts  
Cleaning glass surfaces  
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Protect the Leica DM500 or DM500 B micro-  
scope against damp, vapors, acids, alkalis,  
and corrosive substances. Do not store  
chemicals in the vicinity.  
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Dust and dirt particles should be removed  
with a soft brush or lint-free cotton cloth.  
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Remove dust using a fine, dry and grease-  
free brush made from hair, by blowing with  
a squeeze blower, or by using a vacuum.  
Remove coarse debris with a moistened  
disposable cloth.  
Ϙ
Ϙ
Remove dirt using a clean cloth that has  
been moistened with distilled water.  
Ϙ
Protect the Leica DM500 or DM500 B micro-  
scope from oil and grease. Never grease or  
oil mechanical parts or sliding surfaces.  
Clinging dirt can be removed using petro-  
leum ether or alcohol  
Remove tough dirt using alcohol, chloro-  
form or petroleum ether.  
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Follow the instructions of the disinfectant  
manufacturer.  
Acetone, xylene or nitro-containing thin-  
ners must NOT be used.  
It is advisable to enter a service agreement  
with Leica Service.  
Never use chemicals to clean colored  
surfaces or accessories with rubberized  
parts. This could damage the surfaces,  
and specimens could be contaminated by  
abraded particles.  
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Leica DM 500, DM500 B  
12  
 
Accessories, maintenance and repair  
Accessories  
Maintenance  
Repairs and service work  
Only the following accessories may be used  
with the Leica DM500 or DM500 B microscope:  
Ϙ
The Leica DM500 and DM500 B micro-  
scopes are basically maintenance-free. To  
ensure that they always operate safely and  
reliably, we recommend that you take the  
precaution of contacting the responsible  
service organization.  
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Only original Leica Microsystems spare  
parts may be used.  
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The Leica accessories described in this user  
manual.  
Before opening the instruments, switch off  
the power and unplug the power cable.  
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Other accessories, provided that these have  
been expressly approved by Leica as being  
technically safe in this context.  
Avoid contact with powered electrical  
circuits, which can lead to injury.  
You can arrange for periodic inspections  
or, if appropriate, conclude a mainte-  
nance contract with them.  
Service address  
In case of problems, please contact us as  
follows:  
Ϙ
It is advisable to enter a service agreement  
with Leica Service.  
Leica Microsystems (Switzerland) Ltd.  
Industry Division  
Ϙ
For maintenance and repair, only OEM spare  
parts may be used.  
Max Schmidheiny Strasse 201  
CH-9435 Heerbrugg (Switzerland)  
Phone +41 71 726 33 28  
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Leica DM 500, DM500 B  
13  
 
Electrical data and ambient conditions  
Fuse Replacement  
Environment  
Unplug the instrument before changing any fuses. The  
Leica DM500 has two fuses, which are located behind the  
power cord receptacle.  
Temperature for use  
+10 °C … +40 °C  
-20 °C … +52 °C  
+50 °F … +104 °F  
25 mm on 50 mm hard wood  
100 g / 6 ms  
Storage temperature  
Manipulation shock  
Use only fuse type: 5×20 mm, 1 Amp/250V, Quick acting  
Transport shock (unpacked)  
Transport shock (packed)  
Transport vibrations (unpacked)  
(# 13RFAG30003)  
800 mm free fall  
5–200 Hz / 1.5 g  
Electrical data  
DM500  
Atmospheric pressure "in use" and for storage: 500–1060 mBar  
Input: 100-240 VAC, 50/60 Hz, 5 W (3 W LED)  
Humidity “in use” and for storage:  
Installation Category II (Overvoltage Category)  
Pollution degree 2  
20–90%  
DM500 B  
Input: 100-240 VAC, 50/60 Hz, 0.5-0.2 A OR  
4× 1.2 V AA rechargeable batteries  
Use only 1.2 Volt NiMH type AA rechargeable batteries  
with a maximum of 2700 mAh. Do not use non-recharge-  
able AA batteries.  
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Leica DM 500, DM500 B  
Manual  
14  
 
The Leica DM500, DM500 B  
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Leica DM 500, DM500 B  
Manual  
15  
 
Congratulations!  
Thank you for purchasing the Leica DM500 or  
DM500 B Compound Microscope from Leica  
Microsystems. This model’s exclusive design  
features and full range of accessories make it  
a truly versatile, high-quality instrument for  
educational and routine clinical applications.  
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Leica DM 500, DM500 B  
16  
 
Unpacking  
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Carefully remove the microscope and any  
components from the packing carton.  
Check the components against the planned  
configuration.  
Optional items such as contrast accessories,  
camera adapters, cameras, and carrying  
cases are not shipped as part of the stan-  
dard equipment. These items are delivered  
in separate packages.  
Ϙ
Please do not discard any of the packing  
materials. They should be used for safely  
storing and transporting the instrument  
should the need arise.  
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Leica DM 500, DM500 B  
17  
 
Get Ready!  
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Leica DM 500, DM500 B  
18  
 
Attaching the viewing Tubes  
Tools used  
There are two types of viewing tube.  
Identify which viewing tube you have:  
1. Slightly unscrew the set screw (located on  
top of the stand) with the Allen key tool  
provided.  
Ϙ
Allen key  
2. Insert the dovetail into the stand support  
and tighten the set screw firmly without  
forcing it. This precisely positions the view-  
ing tube onto the optical axis of the micro-  
scope, regardless of the selected rotation.  
Type 1: Leica EZ viewing tube with integrated  
eyepieces.  
Type 2: Standard viewing tube with separate  
eyepieces  
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Leica DM 500, DM500 B  
19  
 
Leica EZ Viewing Tube – integrated eyepieces  
1. To use the captive thumbscrew, completely  
2. Position the Leica EZ viewing tube back in  
place on the stand.  
remove the set screw that comes with the  
stand.  
Leica EZ viewing tube with integrated eyepieces.  
To rotate the Leica EZ viewing tube,  
either loosen the set screw on the stand  
or replace the set screw with the (optional)  
captive thumbscrew.  
3. Attach the captive thumbscrew using the  
wrench tool that comes with the captive  
thumbscrew.  
Be sure the thumbscrew portion of the  
captive thumbscrew is fully loosened  
before attaching it to the stand:  
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Leica DM 500, DM500 B  
20  
 
Leica EZ Viewing Tube – integrated eyepieces (cont.)  
You can now safely rotate the Leica EZ  
viewing tube by loosening the captive  
thumbscrew, rotating the viewing tube, and  
tightening the captive thumbscrew again.  
The eyepieces are already integrated  
with the Leica EZ viewing tube and are  
pre-adjusted for corrected vision; therefore no  
eyepiece adjustment or insertion is necessary.  
Go on to the section "Eyeguards" on page 23.  
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Leica DM 500, DM500 B  
21  
Leica Standard Viewing Tube – separated eyepieces  
1. Insert the eyepieces into the eyetubes.  
2. Lock the eyepieces into the eyetubes by  
tightening the silver screws on the under-  
side of the eyetubes with a standard Cross  
type (Phillips) screwdriver (not provided).  
Standard viewing tube; eyetubes do not yet  
contain the eyepieces  
The standard viewing tube has a rotat-  
able dovetail. Therefore, you can now  
rotate the standard viewing tube freely in any  
orientation.  
The eyepieces will still rotate, but they  
will be captive in the eyetubes.  
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Leica DM 500, DM500 B  
22  
 
Eyeguards  
If you wear eyeglasses for microscope  
If you have purchased a standard micro-  
scope configuration, you will notice that  
viewing, keep the rubber eyeguards  
folded down. If you do not wear eyeglasses,  
you may find it useful to unfold the rubber  
eyeguards in order to help block out ambient  
room light.  
the objectives are already installed on the nose-  
piece, and the substage condenser is already  
installed on the stand. In this case, go to section  
"Operation" on page 26. If you purchased your  
Leica DM500 or DM500 B by components and  
not by standard configuration, please continue  
to section "Installing Objectives" on page 24.  
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Leica DM 500, DM500 B  
23  
 
Installing Objectives and Substage Condenser  
Installing Objectives  
Always rotate the nosepiece by using  
the knurled nosepiece ring.  
Substage Condenser  
The Leica DM500’s and DM500 B's  
substage condenser is pre-installed, pre-  
focused, and pre-centered. No additional instal-  
lation or alignment is required.  
1. As you rotate the nosepiece clockwise,  
attach the objectives by screwing them  
into the nosepiece holes starting with the  
lowest magnification and advancing to the  
highest magnification.  
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Leica DM 500, DM500 B  
24  
 
Get Set!  
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Leica DM 500, DM500 B  
25  
 
Turning on the microscope  
As a safety precaution, the power cord  
has been grounded to the microscope  
base. Never use an adapter between the power  
cord and the power source; it will render the  
grounding feature ineffective.  
If you have a DM500 B with a rechargeable  
base:  
1. Insert the batteries in the underside of the  
base  
Setting the Illumination Intensity  
Set the illumination control knob  
(located on the bottom left of the  
microscope stand) to the lowest setting to  
start. The illumination control knob allows you  
to adjust the intensity of light produced by the  
illumination system.  
2. If the power cord is not already attached,  
attach it securely to the power supply, if  
you use a DM500 B with a rechargeable  
base.  
Work Surface  
Always use your microscope on a hard,  
stable surface.  
3. Plug in the power supply or the optional  
solar panel in order to charge the batteries  
(allow 4 hours minimum to fully charge the  
batteries)  
Power Cord  
If the power cord is not already  
attached, attach it securely to the back  
After being fully charged the batteries  
will provide 6-8 hours of operation.  
of the microscop.  
If necessary, the microscope can func-  
tion without batteries assuming either  
the power supply or optional solar panel is  
plugged in.  
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Leica DM 500, DM500 B  
26  
 
Using the Condenser  
Plug in and Turn on the Microscope  
1. Plug the microscope power cord into a suit-  
able grounded electrical outlet.  
Using the Condenser  
The condenser is furnished with an iris  
diaphragm, which can be adjusted to  
match the effective numerical aperture of each  
objective.  
2. To start, fully open the iris diaphragm of  
the aperture condenser by rotating the  
condenser ring to the extreme right.  
A grounded 3-wire cord is provided.  
1. To open and close the iris diaphragm,  
simply rotate the knurled condenser ring to  
the right or to the left so that the line on  
the rotating ring aligns with the objective  
magnification in use.  
2. Turn the microscope on using the switch on  
the bottom right-hand side of the micro-  
scope stand.  
On/Off switch is located on the bottom right  
hand side of the stand  
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Leica DM 500, DM500 B  
27  
 
Prepare to View a Specimen Slide  
Prepare to View a Specimen Slide  
1. Place a specimen slide on the stage by slid-  
ing the specimen slide forward into the  
slide grips.  
2. Use the X/Y stage control to position the  
slide so a portion of the specimen is under  
the objective in use.  
Slide grips hold the slide in place.  
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Leica DM 500, DM500 B  
28  
 
Focusing  
Focusing  
3. Look into the eyepieces and adjust the illu-  
mination intensity so that it is comfortable  
for viewing.  
1. Rotate the nosepiece (using the knurled  
nosepiece ring) to move the lowest magni-  
fication objective into working position.  
2. Raise the stage by rotating the coarse  
adjustment knob to its positive stop at the  
highest position.  
The Leica DM500 or DM500 B microscope  
stand has been factory-adjusted so that  
the stage focus can be found within 1.5 rotations  
of the fine focus adjustment from this position.  
4. Using the fine adjustment knob, bring the  
specimen into sharp focus.  
Coarse adjustment knob  
Fine adjustment knob  
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Leica DM 500, DM500 B  
29  
 
Viewing Tube Adjustment  
1. Adjust the eyetubes for your interpupillary  
distance. Fold or unfold the eyetubes to  
decrease or increase the distance between  
the eyepieces until you see one illuminated  
circle.  
Leica EZ Viewing Tube  
Standard Tube with One or Two Focusing  
Eyepieces  
If you are using a standard viewing tube with  
one or two focusing eyepieces, you need to  
make some adjustments:  
If you are using a Leica EZ viewing tube, which  
already has the eyepieces integrated with  
the eyetubes, there are no additional adjust-  
ments necessary. Be sure you are wearing your  
eyeglasses or contact lenses. Go on to section  
"Oil Immersion" on page 32.  
1. Set the focusing eyepieces to the "0" diopter  
setting.  
Standard Viewing Tube with Two Fixed  
Eyepieces  
If you are using a standard viewing tube with  
two fixed eyepieces (no focusing eyepieces),  
there are no additional adjustments neces-  
sary. Be sure you are wearing your eyeglasses  
or contact lenses. Go on to section "Oil Immer-  
sion" on page 32.  
The viewing tubes maintain a constant  
tube length for all interpupillary settings.  
This means that a change of interpupillary  
distance does not affect parfocality, magnifica-  
tion, or calibrations that depend on magnifica-  
tion.  
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Leica DM 500, DM500 B  
30  
 
Viewing Tube Adjustment (cont.)  
If you are comfortable wearing your  
corrective lenses (contact lenses or  
eyeglasses) for microscope viewing, leave them  
on and your adjustments will be minimal.  
3. Now switch to look only through the other  
5. Now switch to a high magnification objec-  
tive (not an oil objective) and focus the  
microscope while viewing with both eyes.  
eyepiece (focusing eyepiece) with your  
other eye. This time, focus the specimen by  
using the focusing capability in the focus-  
ing eyepiece.  
The higher magnifications have a shal-  
lower depth of field. Therefore, after  
focusing with a high magnification, you will  
find that when you change to lower magnifi-  
cations, you only have to adjust the fine focus  
slightly, if at all.  
2. Focus on the specimen using the fine  
adjustment knob of the microscope while  
looking only through one of the eyepieces  
(if you have one focusable and one non-  
focusable eyepiece, look through the non-  
focusable eyepiece at this time). To help  
focus, cover or close the other eye.  
Do not raise or lower the stage to focus.  
4. Grip the knurl on the focusing eyepiece  
with one hand and rotate the top of the  
eyepiece with the other hand until the  
specimen is in focus with this eye and this  
focusing eyepiece. This corrects for any  
vision differences between your right eye  
and left eye.  
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Leica DM 500, DM500 B  
31  
Oil Immersion Technique  
Oil Immersion Technique  
1. Find the field of view on the slide that you  
would like to inspect.  
3. Place a drop of Leica Immersion Oil on the  
specimen slide on the area of the specimen  
that you were inspecting.  
5. Raise the stage slowly using the coarse  
focus knob until the drop of oil on the spec-  
imen slide just comes in contact with the  
front of the oil immersion objective lens.  
2. Lower the stage to its lowest position using  
the coarse focus knob.  
4. Swing the oil immersion objective in place  
(the objective will be labeled "OIL")  
6. Grip the knurled nosepiece ring and rock  
the objective back and forth to eliminate  
any air bubbles, then settle the oil objec-  
tive into position with the oil between the  
front lens of the objective and the speci-  
men slide.  
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Leica DM 500, DM500 B  
32  
 
Oil Immersion Technique (cont.)  
7. Look through the microscope and slowly  
rotate the fine focus knob to raise the stage  
until the specimen is in focus.  
8. When finished using the oil immersion  
objective, be sure to clean the front of the  
objective, the specimen slide, and any  
other surfaces that the oil contacted using  
the instructions in section "Care of the  
Microscope" on page 37.  
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Leica DM 500, DM500 B  
33  
Go!  
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Leica DM 500, DM500 B  
34  
 
Ready! Set! Go!  
Now all you need to do is change objectives, set  
the condenser appropriately for the objective  
magnification in use, and enjoy the view!  
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Leica DM 500, DM500 B  
35  
 
Care of the Microscope  
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Leica DM 500, DM500 B  
Manual  
36  
 
General maintenance  
General  
Ϙ
The cord wrap allows you to wrap the cord  
in such a way that only the length you need  
is extended.  
Ϙ
Ϙ
Ϙ
If any optical surface becomes coated with  
dust or dirt, clean the surface by blowing it  
off with a syringe or brushing it off with a  
camel hair brush before attempting to wipe  
the surface clean.  
Always carry the microscope using two  
hands. There is a handle on the back of  
the microscope and an undercut in the front for  
this purpose.  
Optical surfaces should be cleaned with a  
lint-free cloth, lens tissue or a cotton swab  
moistened with methanol or a commer-  
cially available glass cleaner. Do not use  
alcohol.  
It is very important to avoid the excessive  
use of solvents, so use them sparingly. The  
lint-free cloth, lens tissue or cotton swab  
should be moistened with solvent, but  
not be wet enough for the solvent to seep  
around the lens.  
Ϙ
Ϙ
Keep all optical components clean. Clean-  
liness is important for maintaining good  
optical performance.  
The microscope should always be covered  
with the plastic dust cover (provided with  
the instrument) when it is not in use.  
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Leica DM 500, DM500 B  
37  
 
General maintenance (cont.)  
Ϙ
No part of the microscope is quite so vulner-  
able to collecting dirt, dust, and oil as the  
front lens of the objective. Whenever you  
encounter lack of contrast, cloudiness or  
poor definition, carefully check the condi-  
tion of the front lens with a magnifier.  
Make sure that the cotton tip contacts the  
concave lens surface. Check the objective  
with a magnifier after cleaning.  
Ϙ
If you need to remove the microscope’s  
viewing body, be careful not to accidentally  
touch the outer lens surface (located on  
the underside of the body). Fingerprints on  
this surface will reduce image clarity. This  
lens can be cleaned in the same manner as  
objectives and eyepieces.  
Ϙ
The lower magnification objectives have  
fairly large front lenses and can be cleaned  
with a cloth or lens tissue wrapped around  
a finger and moistened with methanol.  
Ϙ
Cleaning 40× and 100× objectives requires  
more care. Note: To achieve the high degree  
of flatness obtained with higher magnifica-  
tion objectives, the objective has a small  
concave front lens of fairly short radius or  
curvature. The surface of this front lens can  
be readily cleaned with a toothpick covered  
with a cotton tip, or with a small cotton  
swab. Moisten the cotton with methanol  
and squeeze it almost dry (do not use alco-  
hol). Wipe the front lens lightly without  
applying undue force or scrubbing action.  
Illumination  
The Leica DM500 and DM500 B uses LED  
Ϙ
illumination. Therefore, no lamp changing  
is required for the life of the microscope.  
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Leica DM 500, DM500 B  
38  
Dimensions  
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Leica DM 500, DM500 B  
39  
 
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Leica DM 500, DM500 B  
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